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The map label for this gene is ynjE [H]

Identifier: 218548675

GI number: 218548675

Start: 1347434

End: 1348741

Strand: Reverse

Name: ynjE [H]

Synonym: EFER_1309

Alternate gene names: 218548675

Gene position: 1348741-1347434 (Counterclockwise)

Preceding gene: 218548676

Following gene: 218548673

Centisome position: 29.39

GC content: 53.59

Gene sequence:

>1308_bases
ATGAAACGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGC
GAAGCCTCTTACTCTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTTATAACGGCT
GGCCACAAACCTTAAATGGCCCTTCCGGTCATGAACCTGCCGCCTTAAACCTTTCTGCTAGCTGGCTCGACAAAATGAGC
ACCGAACAACTCAACGAGTGGATCAAGCAACATAACCTGAAAGCCGATGCTCCGGTGGCGCTGTACGGTAATGACAAAGA
TGTCGACGCCGTCAAAACGCGCCTGCAAAAAGCAGGCTTAACGCATATCTCCATCCTGAGTGACGCGCTAAGCGAACCTT
CCCGTCTGCAAAAACTGCCGCACTTTGAGCAGCTGGTTTACCCGCAATGGCTACATGACCTGCAACAGGGTAAAGAGGTT
ACGGCGAAACCTGCCGGTGACTGGAAAGTCATTGAAGCGGCCTGGGGCGCTCCTAAGCTTTACCTTATCAGCCATATTCC
CGGCGCGGATTACATCGATACCAATGAAGTGGAAAGCGAACCGCTGTGGAATAAAGTCTCTGATGAACAACTGAAAGCGA
TGCTGGCAAAGCATGGCATTCGTCATGACACCACGGTGATTCTATATGGTCGTGATGTATACGCAGCAGCGCGTGTAGCA
CAGATTATGCTCTATGCAGGGGTGAAAGATGTGCGCCTGCTGGACGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCC
TGTTGAGCGCGGAACGCCACCGAAAGTGAAAGCTGAACCGGATTTCGGCGTGAAGATCCCGGCGCAACCGCAGTTGATGC
TTGATATGGAACAGGCCCGTGGGCTGCTGCATCGCCAGGATGCATCGTTGGTGAGCATTCGTTCGTGGCCAGAATTTATC
GGTACGACCAGTGGTTACAGCTATATTAAGCCAAAAGGTGAAATAGCCGGAGCCCGTTGGGGACACGCTGGTAGCGACTC
GACGCATATGGAAGATTTCCATAACCCGGATGGCACCATGCGTAGCGCCGATGATATTGCCGCTATGTGGAAAGCATGGA
ATATCAAACCAGATCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTACGCACGAGCA
ATGGGCTGGAATAACGTCTCCGTTTACGACGGCGGCTGGTACGAATGGAGCAGCGATCCGAAAAATCCCGTAGCAACCGG
TGAGCGCGGCCCGGACAGCAGCAAATAA

Upstream 100 bases:

>100_bases
GCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCCCAATGTGTCAGTTCAGAATGGCGCACTCAAAA
CTACAATGTCGGGATTTTCG

Downstream 100 bases:

>100_bases
CATTGCAATTACTGGCGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAA
CGCACCAAATACCCACGCAA

Product: thiosulfate sulfur transferase / thiosulfate binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMS
TEQLNEWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGLTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAAARVA
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFI
GTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDIAAMWKAWNIKPDQQVSFYCGTGWRASETFMYARA
MGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK

Sequences:

>Translated_435_residues
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMS
TEQLNEWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGLTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAAARVA
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFI
GTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDIAAMWKAWNIKPDQQVSFYCGTGWRASETFMYARA
MGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK
>Mature_435_residues
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMS
TEQLNEWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGLTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAAARVA
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFI
GTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDIAAMWKAWNIKPDQQVSFYCGTGWRASETFMYARA
MGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK

Specific function: Unknown

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=302, Percent_Identity=26.4900662251656, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI61835204, Length=300, Percent_Identity=24.6666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI194473681, Length=300, Percent_Identity=24.6666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI194473668, Length=300, Percent_Identity=24.6666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI87081967, Length=435, Percent_Identity=98.6206896551724, Blast_Score=884, Evalue=0.0,
Organism=Escherichia coli, GI87082121, Length=249, Percent_Identity=22.8915662650602, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 48183; Mature: 48183

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCCHHHCCCCCCCCC
PSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGL
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
THISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEVTAKPAGDWKVIEAAWGAPKL
HHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCEE
YLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAAARVA
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQAR
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCEECCCCCHHEEEHHHHC
GLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTM
CHHHHCCCCEEEHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
RSADDIAAMWKAWNIKPDQQVSFYCGTGWRASETFMYARAMGWNNVSVYDGGWYEWSSDP
CCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHEEEHHHCCCCCEEEECCCEEECCCCC
KNPVATGERGPDSSK
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCCHHHCCCCCCCCC
PSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGL
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
THISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEVTAKPAGDWKVIEAAWGAPKL
HHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCEE
YLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAAARVA
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQAR
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCEECCCCCHHEEEHHHHC
GLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTM
CHHHHCCCCEEEHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
RSADDIAAMWKAWNIKPDQQVSFYCGTGWRASETFMYARAMGWNNVSVYDGGWYEWSSDP
CCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHEEEHHHCCCCCEEEECCCEEECCCCC
KNPVATGERGPDSSK
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9097039 [H]