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The map label for this gene is yebU [H]

Identifier: 218548605

GI number: 218548605

Start: 1276685

End: 1278124

Strand: Reverse

Name: yebU [H]

Synonym: EFER_1239

Alternate gene names: 218548605

Gene position: 1278124-1276685 (Counterclockwise)

Preceding gene: 218548606

Following gene: 218548604

Centisome position: 27.85

GC content: 51.67

Gene sequence:

>1440_bases
GTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTTTGACGCAAATGCGCGAAGCAATGCCTTCGACGCTCTCTTTTGA
TGATTTTCTTGCCGCCTGTCAGCGCCCGTTGCGCCGCAGCATTCGCGTTAATACGCTGAAAATCTCCGTTGCTGATTTCC
TGCAATTAACCGCTCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACGAT
GAAGATGCATTGCCATTGGGTAGTACAGCAGAGCATTTAAGCGGCCTGTTTTATATTCAGGAAGCCAGTTCAATGTTGCC
CGTTGCCGCCTTGTTTGCTGACGGTAATTCACCACAGCGGGTGATGGATGTCGCTGCTGCGCCAGGCTCCAAAACGACGC
AAATTGCTGCGCGGATGAATAACGAAGGGGCGATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCC
AATATCAGCCGCTGTGGCATCCGTAATGTTGCGCTCACGCATTTTGATGGCCGTGTATTTGGTGCGGCATTACCAGAAAT
GTTCGATGCCATTTTGCTGGACGCCCCCTGCTCCGGCGAAGGCGTGGTCCGTAAAGATCCCGATGCGCTGAAAAACTGGT
CACCAGAAAGCAATCAGGAAATCGCTGCCACCCAGCGTGAACTGATCGACAGTGCCTTTCATGCATTACGTCCTGGCGGT
ACGCTGGTTTACTCAACCTGTACCTTAAATCGGGAAGAAAATGAAGCCGTCTGCCTGTGGCTGAAAGAGACTTACCCCGA
CGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGCGGACAAGGCGCTGACCGAGGAAGGTTTTCTTCACGTTT
TCCCACAAATTTACGACTGTGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCTATTCCCGCATTGCCTGCGCCA
AAATACAAAGTCGGTAATTTCCCATTTATCCCGGTGAAAAATCGCGAAGCTGGACAAATTTGTCAGGCGGCTGCAAGTGT
TGGCTTAACCTGGGATGAAAACCTGCGACTCTGGCAACGCGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCT
TGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAAACACATAACAAAGGTTATCGCTGGCAGCATGAA
GCGGTTATTGCCCTTGCCTCCCCCGACAATGTGAACGCTTTTGAACTGACACCGCAGAAAGCGGAGGAGTGGTATCGCGG
GCGCGATGTTTACCCGCAAGCCGCGCCAGTTGCGGATGATGTATTGGTTACTTTCCAGCATCAACCGATTGGTTTAGCCA
AACGGATTGGTTCGCGACTGAAAAACAGCTACCCGCGTGAACTGGTGCGCGATGGGAAACTTTTTACCGGTAACGCCTGA

Upstream 100 bases:

>100_bases
GGAACTGCGCTGCCCAAATAATGTCCATTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTT
TTTGCCAGTGGTATATGCTC

Downstream 100 bases:

>100_bases
AAGCGCACAAAAAAAAGCGCACGTTTTGACTGGCGCATTCGGCTACCTCAACTAGGCTGAAAAATGGCGCGATCGGACTG
GTCGTACCACAACCGACAGC

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDD
EDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNSPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
NISRCGIRNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
TLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGADKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAP
KYKVGNFPFIPVKNREAGQICQAAASVGLTWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHE
AVIALASPDNVNAFELTPQKAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA

Sequences:

>Translated_479_residues
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDD
EDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNSPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
NISRCGIRNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
TLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGADKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAP
KYKVGNFPFIPVKNREAGQICQAAASVGLTWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHE
AVIALASPDNVNAFELTPQKAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA
>Mature_478_residues
AQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDDE
DALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNSPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHAN
ISRCGIRNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGGT
LVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGADKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPK
YKVGNFPFIPVKNREAGQICQAAASVGLTWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEA
VIALASPDNVNAFELTPQKAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=310, Percent_Identity=35.8064516129032, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI76150623, Length=310, Percent_Identity=35.8064516129032, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI11545785, Length=167, Percent_Identity=38.3233532934132, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI32698918, Length=173, Percent_Identity=35.2601156069364, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI39995082, Length=206, Percent_Identity=33.0097087378641, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI301336155, Length=206, Percent_Identity=33.0097087378641, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI40316918, Length=210, Percent_Identity=30.4761904761905, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI270288816, Length=332, Percent_Identity=29.2168674698795, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI23199998, Length=327, Percent_Identity=29.0519877675841, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI8922322, Length=327, Percent_Identity=28.7461773700306, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI270288818, Length=327, Percent_Identity=28.7461773700306, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI87081985, Length=479, Percent_Identity=96.8684759916493, Blast_Score=964, Evalue=0.0,
Organism=Escherichia coli, GI2367212, Length=199, Percent_Identity=32.6633165829146, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17536757, Length=320, Percent_Identity=33.4375, Blast_Score=166, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI25143471, Length=193, Percent_Identity=32.6424870466321, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71998419, Length=323, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=290, Percent_Identity=37.5862068965517, Blast_Score=166, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6319447, Length=206, Percent_Identity=32.0388349514563, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI22024126, Length=333, Percent_Identity=34.8348348348348, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21355201, Length=208, Percent_Identity=33.6538461538462, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24668781, Length=175, Percent_Identity=35.4285714285714, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356513, Length=177, Percent_Identity=33.8983050847458, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 53267; Mature: 53136

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPY
CCCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHCCC
GWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNSPQR
CCEECCCCCCCCCCEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCHHH
VMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGIRNVALTHFDGRVF
HHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCEEH
GAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
HHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGADKALTEEGFLHVFPQIYDC
EEEEEEEECCCCCCCEEEEEEECCCCCHHHEEECCCCCCCCCHHHCCCCCEEECCEEECC
EGFFVARLRKTQAIPALPAPKYKVGNFPFIPVKNREAGQICQAAASVGLTWDENLRLWQR
CCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEC
DKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALASPDNVNAFELTPQK
CCCEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEEEEEEEEEECCCCCCEEEECHHH
AEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCC
>Mature Secondary Structure 
AQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPY
CCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHCCC
GWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNSPQR
CCEECCCCCCCCCCEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCHHH
VMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGIRNVALTHFDGRVF
HHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCEEH
GAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
HHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGDLFPGADKALTEEGFLHVFPQIYDC
EEEEEEEECCCCCCCEEEEEEECCCCCHHHEEECCCCCCCCCHHHCCCCCEEECCEEECC
EGFFVARLRKTQAIPALPAPKYKVGNFPFIPVKNREAGQICQAAASVGLTWDENLRLWQR
CCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEC
DKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALASPDNVNAFELTPQK
CCCEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEEEEEEEEEECCCCCCEEEECHHH
AEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA