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The map label for this gene is fliA [H]

Identifier: 218548541

GI number: 218548541

Start: 1212730

End: 1213446

Strand: Direct

Name: fliA [H]

Synonym: EFER_1171

Alternate gene names: 218548541

Gene position: 1212730-1213446 (Clockwise)

Preceding gene: 218548540

Following gene: 218548542

Centisome position: 26.43

GC content: 55.09

Gene sequence:

>717_bases
GTGAATTCACTGTATACCGCTGAAGGCGTAATGGATAAACACTCGCTGTGGCAACGCTATGTGCCGCTGGTGCGACACGA
AGCACTGCGCCTACAGGTGCGGCTGCCCGCGAGCGTAGAACTGGACGACCTGCTACAGGCAGGCGGCATCGGGTTATTGA
ACGCAGTAGACCGCTATGACGCTCTGCAAGGAACGGCATTTACAACTTACGCAGTGCAGCGTATCCGAGGGGCAATGCTG
GATGAACTACGCAGCCGTGACTGGGCGCCGCGTAGTGTCCGCCGCAATGCGCGCGAAGTGGCGCAGGCAATAGGGCAACT
GGAACAAGAGTTAGGGCGCAACGCCAGCGAAACGGAAGTGGCAAAACGACTGGGGATCTCGACAGAAGAGTATCGCCAGA
TGTTGCTCGATACCAATAACAGTCAGTTGTTTTCCTATGACGAATGGCGGGAAGAGCATGGTGACAGTGCTGAACTGATC
ACCGAAGAGCATCAGCAGGCTAATCCGCTGCACCAGTTGCTGGAGAGTGATCTGCGCCAGCAAGTAATGGATGCCATCGA
ACGTTTGCCAGAGCGTGAAAAATTGGTGCTTACCCTTTACTACCAGGAAGAGCTGAATCTCAAAGAGATCGGCGCGGTAC
TGGAAGTGGGTGAGTCGCGCGTCAGTCAGTTGCATAGCCAGGCCATCAAACGATTACGCAGCAAACTGGGTAGGTGA

Upstream 100 bases:

>100_bases
CTGCAAATAACCCCCCATTTCACCCACTATTCATCCGATTAAATCCTCTACAGAAACGGATAATCATGCCGATAACACAT
CTAACGCAGGGCTGTTTATC

Downstream 100 bases:

>100_bases
ATACGGTAACCACATTGCCGTCAAATGCCGCACAACGAATTGACTACCAGGAGTTATCATGACGGTGCAGCAAAGCAAAC
GACGGCCTCTGAGCCGCTAC

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDLLQAGGIGLLNAVDRYDALQGTAFTTYAVQRIRGAML
DELRSRDWAPRSVRRNAREVAQAIGQLEQELGRNASETEVAKRLGISTEEYRQMLLDTNNSQLFSYDEWREEHGDSAELI
TEEHQQANPLHQLLESDLRQQVMDAIERLPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRSKLGR

Sequences:

>Translated_238_residues
MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDLLQAGGIGLLNAVDRYDALQGTAFTTYAVQRIRGAML
DELRSRDWAPRSVRRNAREVAQAIGQLEQELGRNASETEVAKRLGISTEEYRQMLLDTNNSQLFSYDEWREEHGDSAELI
TEEHQQANPLHQLLESDLRQQVMDAIERLPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRSKLGR
>Mature_238_residues
MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDLLQAGGIGLLNAVDRYDALQGTAFTTYAVQRIRGAML
DELRSRDWAPRSVRRNAREVAQAIGQLEQELGRNASETEVAKRLGISTEEYRQMLLDTNNSQLFSYDEWREEHGDSAELI
TEEHQQANPLHQLLESDLRQQVMDAIERLPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRSKLGR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=238, Percent_Identity=90.7563025210084, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1789448, Length=208, Percent_Identity=26.4423076923077, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27348; Mature: 27348

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDLLQAGGIGLLNAVDRYD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCCHHHHHHHHHH
ALQGTAFTTYAVQRIRGAMLDELRSRDWAPRSVRRNAREVAQAIGQLEQELGRNASETEV
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AKRLGISTEEYRQMLLDTNNSQLFSYDEWREEHGDSAELITEEHQQANPLHQLLESDLRQ
HHHHCCCHHHHHHHHHCCCCCCEECHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHH
QVMDAIERLPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRSKLGR
HHHHHHHHCCCCCCEEEEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDLLQAGGIGLLNAVDRYD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCCHHHHHHHHHH
ALQGTAFTTYAVQRIRGAMLDELRSRDWAPRSVRRNAREVAQAIGQLEQELGRNASETEV
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AKRLGISTEEYRQMLLDTNNSQLFSYDEWREEHGDSAELITEEHQQANPLHQLLESDLRQ
HHHHCCCHHHHHHHHHCCCCCCEECHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHH
QVMDAIERLPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRSKLGR
HHHHHHHHCCCCCCEEEEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]