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The map label for this gene is yliE [H]

Identifier: 218548529

GI number: 218548529

Start: 1196881

End: 1199229

Strand: Direct

Name: yliE [H]

Synonym: EFER_1159

Alternate gene names: 218548529

Gene position: 1196881-1199229 (Clockwise)

Preceding gene: 218548528

Following gene: 218548530

Centisome position: 26.08

GC content: 36.1

Gene sequence:

>2349_bases
ATGTATAGTATTTCCGAAAAAGTTAACTCCAGTTTCAAATTCGCAATTACTGTCGCCATTGCTGTTTATATTTTGCTGAG
TCTTTTTACTGCCTTTGACTTTCACAAAAAAATTGCAGAAAGTTCTTCAAAGAATCACCAGGAATTATTACGGAACAATT
TACGCAATTATTTTTCAAAAGTAGAAAAAGAAGCTGATGCGCTAAAAGATGCACTTTATCTGTTACAGGATGAAGAAGAG
ATCAAACGTGCATTAATTCATCGTATGGCAAAAATAGAGGGCATAAATCTGGTTGGCCTGATGATGAATAATGGTAAATA
TTATAGCTTCATTCGCACGCCCGGTGGTGAAATAAAACTACAGGCGAAATTTGTCCCTGGCAGACCACTCACTGGCGCCG
ATGGTGAAGTAATTGATGAGAACTTTAACCCTCTATCTCGTCCGTGGAATGACATTCCTCCAGGTGCGGTTAGCAAATGG
GCATCATGGTACGATTGTTATGGTATGCCCGGTAAAAAATGCTTTACATTCTCTTTGTCTCCTTCAGAAAATGAAAAAAA
CAATTTTGCAATCAAACTTATTCATTTGGATCTGGATGAGAAATATTTGACGAATTTTTTAAATGCTCAGGGGAAACCAG
GCGATATTATCTTTTTAGAAAATAATGGACGAATTATCGGCGGGAATGAGGAATCAACGCTACATCTTATCGTTAAAGAT
ACTAATGGTGAATACAGAACCAGTAATGATACTGATGAGTATTTTGAGAAAGTCATTATATTACCTAAACTTCCTGATGT
TAAATTTCACTATTTCTATTCGCTTAACAGTAAGTTATCCTATCTGAATAAAAGTTTTGTCTTAATGTTTATGAGTGGAA
TTGTCTGCTTCTTTCTGCTCTGGTACATCATCATTGTAATTACCCGAAAAATTAGTGGTGAGTCAGGTAAACTGGTGCGT
AATCTGGAACATTTAGGCAATTCAGTCTACAAGGAAGATAAAGTAGATATTTCAACCGAAGATAGCCGAGAAATTGTTGA
ACTTAAGAAAATTATCAATACCATTGGTGAGAAATATAAAGAAAGAATTGAACGTATGTTAAATTTGGTTTCCTACGAAC
AGGGGACTAGTTTTTATATCAATACAGCGGTTATTGAAACCAATAACAAAGATTATGTTGCAGCAGGAATGATTTGTCTT
TACGGGCTTGAGTTTGCTGAAGCCATTTATGGTAGCGAGAAATACAATGCTATTATTAGTGAATTAAAGTCGAAAATCTC
TGCAAAATATTCATCTCACTGTGATATTATTAAAATATCAAACGACAGATATTTATTGCTTTGCTATACAGATTGTGAAG
AATTTATTTCTAACTTCTCGGTTCTTAATCTCCCGGAAAATATATTTTGCCTGCGGAATATTTATGTGCATAAAACAGCA
CTTTGTGAAACCTTTTCCGGGAAAGATGTGTCTATATATGAAGGTAAATTAAAATTAGCGATGACAGCTATGCGCGAAAA
TAAACAATCTGAATTTCTGTATTATGATGATATTAAACTTCACGAATTAGATCAAAAGGTATGGGTGGCAAGCAATATAA
AACATGCAATCAGCCGTGGTGAATTTTATCTGGTGTATCAGCCGATCGTTAATATCGACAGCAACGAAGTCGTGGGAGCG
GAAGCGCTGTGCCGGTGGAACTCAGCTGAGTATGGTTTTATCTCCCCGGCTGTATTTGTGTCTATCGCTGAAGATATTGG
ACTGATTAAAGAGCTAGGTGATTATATACTTGAGAATGCTATTCGCGAATTCAGCCAGTTCAGTAATGAGTATGTGATTA
GTGATGAGTTTTTATTGCATATTAATGTTTCTCCCTGGCAGTTAAATGACTTCGATTTTCATCATCGTGTTTTGCAGTTG
ATTAAAGATTTCAATATTAATGCCGGACAAATTTGTCTTGAGATCACTGAATCAGCAGTAAAAGAGATTAATGACAGCTT
TTATAACAATATTATTGTGTTAAAAGGTAACGGTATCAAAATTGCGCTGGACGATTTTGGTAGTGGGCTTGCTGACTTGA
AGAAACTCTACAAAGTTAAACCCGACAGTATCAAAATCGACTCCGAGTTTACTTCCGGGGTATTTGATGAAACCAACCGG
ATAGTATGGTTTATTTCCAGTTTTGCCCGAGAAGAAAATCTACCCGTTATCGCTGAAGGTGTGGAAACAGAACATCAGGC
AAAAGAATTACAAAAATTGGGCTTCTCACAGGTACAAGGATACCTGTATCAAAAGCCGTTACCATTCTCTGAATGGCATA
TCGATAGCAAACCACAGAAAAGAAGTTAA

Upstream 100 bases:

>100_bases
GTTCGAATTGCAGTCGCAGTACCTGCAACTTGAAGTGTGTGGGATATAAAAATTATCTTCTTAATATAGTTTCCAGCATA
TCATTTAGAACTCATCATTT

Downstream 100 bases:

>100_bases
GACAATGTTTAAGTATAAGAATAAAGCAGCAGGTATTAGTGTGCTATGTTTTGTTTTTATATCAGCGATAACATGTACGC
TATATACTGAAAATGTTAAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 782; Mature: 782

Protein sequence:

>782_residues
MYSISEKVNSSFKFAITVAIAVYILLSLFTAFDFHKKIAESSSKNHQELLRNNLRNYFSKVEKEADALKDALYLLQDEEE
IKRALIHRMAKIEGINLVGLMMNNGKYYSFIRTPGGEIKLQAKFVPGRPLTGADGEVIDENFNPLSRPWNDIPPGAVSKW
ASWYDCYGMPGKKCFTFSLSPSENEKNNFAIKLIHLDLDEKYLTNFLNAQGKPGDIIFLENNGRIIGGNEESTLHLIVKD
TNGEYRTSNDTDEYFEKVIILPKLPDVKFHYFYSLNSKLSYLNKSFVLMFMSGIVCFFLLWYIIIVITRKISGESGKLVR
NLEHLGNSVYKEDKVDISTEDSREIVELKKIINTIGEKYKERIERMLNLVSYEQGTSFYINTAVIETNNKDYVAAGMICL
YGLEFAEAIYGSEKYNAIISELKSKISAKYSSHCDIIKISNDRYLLLCYTDCEEFISNFSVLNLPENIFCLRNIYVHKTA
LCETFSGKDVSIYEGKLKLAMTAMRENKQSEFLYYDDIKLHELDQKVWVASNIKHAISRGEFYLVYQPIVNIDSNEVVGA
EALCRWNSAEYGFISPAVFVSIAEDIGLIKELGDYILENAIREFSQFSNEYVISDEFLLHINVSPWQLNDFDFHHRVLQL
IKDFNINAGQICLEITESAVKEINDSFYNNIIVLKGNGIKIALDDFGSGLADLKKLYKVKPDSIKIDSEFTSGVFDETNR
IVWFISSFAREENLPVIAEGVETEHQAKELQKLGFSQVQGYLYQKPLPFSEWHIDSKPQKRS

Sequences:

>Translated_782_residues
MYSISEKVNSSFKFAITVAIAVYILLSLFTAFDFHKKIAESSSKNHQELLRNNLRNYFSKVEKEADALKDALYLLQDEEE
IKRALIHRMAKIEGINLVGLMMNNGKYYSFIRTPGGEIKLQAKFVPGRPLTGADGEVIDENFNPLSRPWNDIPPGAVSKW
ASWYDCYGMPGKKCFTFSLSPSENEKNNFAIKLIHLDLDEKYLTNFLNAQGKPGDIIFLENNGRIIGGNEESTLHLIVKD
TNGEYRTSNDTDEYFEKVIILPKLPDVKFHYFYSLNSKLSYLNKSFVLMFMSGIVCFFLLWYIIIVITRKISGESGKLVR
NLEHLGNSVYKEDKVDISTEDSREIVELKKIINTIGEKYKERIERMLNLVSYEQGTSFYINTAVIETNNKDYVAAGMICL
YGLEFAEAIYGSEKYNAIISELKSKISAKYSSHCDIIKISNDRYLLLCYTDCEEFISNFSVLNLPENIFCLRNIYVHKTA
LCETFSGKDVSIYEGKLKLAMTAMRENKQSEFLYYDDIKLHELDQKVWVASNIKHAISRGEFYLVYQPIVNIDSNEVVGA
EALCRWNSAEYGFISPAVFVSIAEDIGLIKELGDYILENAIREFSQFSNEYVISDEFLLHINVSPWQLNDFDFHHRVLQL
IKDFNINAGQICLEITESAVKEINDSFYNNIIVLKGNGIKIALDDFGSGLADLKKLYKVKPDSIKIDSEFTSGVFDETNR
IVWFISSFAREENLPVIAEGVETEHQAKELQKLGFSQVQGYLYQKPLPFSEWHIDSKPQKRS
>Mature_782_residues
MYSISEKVNSSFKFAITVAIAVYILLSLFTAFDFHKKIAESSSKNHQELLRNNLRNYFSKVEKEADALKDALYLLQDEEE
IKRALIHRMAKIEGINLVGLMMNNGKYYSFIRTPGGEIKLQAKFVPGRPLTGADGEVIDENFNPLSRPWNDIPPGAVSKW
ASWYDCYGMPGKKCFTFSLSPSENEKNNFAIKLIHLDLDEKYLTNFLNAQGKPGDIIFLENNGRIIGGNEESTLHLIVKD
TNGEYRTSNDTDEYFEKVIILPKLPDVKFHYFYSLNSKLSYLNKSFVLMFMSGIVCFFLLWYIIIVITRKISGESGKLVR
NLEHLGNSVYKEDKVDISTEDSREIVELKKIINTIGEKYKERIERMLNLVSYEQGTSFYINTAVIETNNKDYVAAGMICL
YGLEFAEAIYGSEKYNAIISELKSKISAKYSSHCDIIKISNDRYLLLCYTDCEEFISNFSVLNLPENIFCLRNIYVHKTA
LCETFSGKDVSIYEGKLKLAMTAMRENKQSEFLYYDDIKLHELDQKVWVASNIKHAISRGEFYLVYQPIVNIDSNEVVGA
EALCRWNSAEYGFISPAVFVSIAEDIGLIKELGDYILENAIREFSQFSNEYVISDEFLLHINVSPWQLNDFDFHHRVLQL
IKDFNINAGQICLEITESAVKEINDSFYNNIIVLKGNGIKIALDDFGSGLADLKKLYKVKPDSIKIDSEFTSGVFDETNR
IVWFISSFAREENLPVIAEGVETEHQAKELQKLGFSQVQGYLYQKPLPFSEWHIDSKPQKRS

Specific function: Overexpression reduces biofilm formation. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787055, Length=773, Percent_Identity=38.8098318240621, Blast_Score=583, Evalue=1e-167,
Organism=Escherichia coli, GI87081921, Length=274, Percent_Identity=29.1970802919708, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI87081980, Length=270, Percent_Identity=35.9259259259259, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1787541, Length=296, Percent_Identity=29.7297297297297, Blast_Score=136, Evalue=6e-33,
Organism=Escherichia coli, GI226510982, Length=294, Percent_Identity=30.2721088435374, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1790496, Length=245, Percent_Identity=31.0204081632653, Blast_Score=128, Evalue=2e-30,
Organism=Escherichia coli, GI87081845, Length=253, Percent_Identity=32.0158102766798, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1788502, Length=245, Percent_Identity=31.8367346938775, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI87081743, Length=246, Percent_Identity=29.6747967479675, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI1786507, Length=246, Percent_Identity=30.8943089430894, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788849, Length=267, Percent_Identity=29.9625468164794, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87082096, Length=252, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1787410, Length=227, Percent_Identity=28.6343612334802, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633 [H]

Pfam domain/function: PF00563 EAL [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 89570; Mature: 89570

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSISEKVNSSFKFAITVAIAVYILLSLFTAFDFHKKIAESSSKNHQELLRNNLRNYFSK
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VEKEADALKDALYLLQDEEEIKRALIHRMAKIEGINLVGLMMNNGKYYSFIRTPGGEIKL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEEECCCCCEEE
QAKFVPGRPLTGADGEVIDENFNPLSRPWNDIPPGAVSKWASWYDCYGMPGKKCFTFSLS
EEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECC
PSENEKNNFAIKLIHLDLDEKYLTNFLNAQGKPGDIIFLENNGRIIGGNEESTLHLIVKD
CCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCEEEEEEEE
TNGEYRTSNDTDEYFEKVIILPKLPDVKFHYFYSLNSKLSYLNKSFVLMFMSGIVCFFLL
CCCCCCCCCCHHHHHHHEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
WYIIIVITRKISGESGKLVRNLEHLGNSVYKEDKVDISTEDSREIVELKKIINTIGEKYK
HHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ERIERMLNLVSYEQGTSFYINTAVIETNNKDYVAAGMICLYGLEFAEAIYGSEKYNAIIS
HHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ELKSKISAKYSSHCDIIKISNDRYLLLCYTDCEEFISNFSVLNLPENIFCLRNIYVHKTA
HHHHHHHHHHCCCCCEEEECCCCEEEEEECCHHHHHCCCEEECCCCHHHHHHHHHHHHHH
LCETFSGKDVSIYEGKLKLAMTAMRENKQSEFLYYDDIKLHELDQKVWVASNIKHAISRG
HHHCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEECCEEEEHHCCHHHHHHHHHHHHCCC
EFYLVYQPIVNIDSNEVVGAEALCRWNSAEYGFISPAVFVSIAEDIGLIKELGDYILENA
CEEEEEECEECCCCCCEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IREFSQFSNEYVISDEFLLHINVSPWQLNDFDFHHRVLQLIKDFNINAGQICLEITESAV
HHHHHHCCCCEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KEINDSFYNNIIVLKGNGIKIALDDFGSGLADLKKLYKVKPDSIKIDSEFTSGVFDETNR
HHHHHHHHCCEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCCEEECCHHHCCCCCCCCC
IVWFISSFAREENLPVIAEGVETEHQAKELQKLGFSQVQGYLYQKPLPFSEWHIDSKPQK
EEEEEEHHHCCCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCC
RS
CC
>Mature Secondary Structure
MYSISEKVNSSFKFAITVAIAVYILLSLFTAFDFHKKIAESSSKNHQELLRNNLRNYFSK
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VEKEADALKDALYLLQDEEEIKRALIHRMAKIEGINLVGLMMNNGKYYSFIRTPGGEIKL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEEECCCCCEEE
QAKFVPGRPLTGADGEVIDENFNPLSRPWNDIPPGAVSKWASWYDCYGMPGKKCFTFSLS
EEEECCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECC
PSENEKNNFAIKLIHLDLDEKYLTNFLNAQGKPGDIIFLENNGRIIGGNEESTLHLIVKD
CCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCEEEEEEEE
TNGEYRTSNDTDEYFEKVIILPKLPDVKFHYFYSLNSKLSYLNKSFVLMFMSGIVCFFLL
CCCCCCCCCCHHHHHHHEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
WYIIIVITRKISGESGKLVRNLEHLGNSVYKEDKVDISTEDSREIVELKKIINTIGEKYK
HHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ERIERMLNLVSYEQGTSFYINTAVIETNNKDYVAAGMICLYGLEFAEAIYGSEKYNAIIS
HHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ELKSKISAKYSSHCDIIKISNDRYLLLCYTDCEEFISNFSVLNLPENIFCLRNIYVHKTA
HHHHHHHHHHCCCCCEEEECCCCEEEEEECCHHHHHCCCEEECCCCHHHHHHHHHHHHHH
LCETFSGKDVSIYEGKLKLAMTAMRENKQSEFLYYDDIKLHELDQKVWVASNIKHAISRG
HHHCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEECCEEEEHHCCHHHHHHHHHHHHCCC
EFYLVYQPIVNIDSNEVVGAEALCRWNSAEYGFISPAVFVSIAEDIGLIKELGDYILENA
CEEEEEECEECCCCCCEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IREFSQFSNEYVISDEFLLHINVSPWQLNDFDFHHRVLQLIKDFNINAGQICLEITESAV
HHHHHHCCCCEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KEINDSFYNNIIVLKGNGIKIALDDFGSGLADLKKLYKVKPDSIKIDSEFTSGVFDETNR
HHHHHHHHCCEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCCEEECCHHHCCCCCCCCC
IVWFISSFAREENLPVIAEGVETEHQAKELQKLGFSQVQGYLYQKPLPFSEWHIDSKPQK
EEEEEEHHHCCCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCC
RS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]