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The map label for this gene is otsB [H]

Identifier: 218548497

GI number: 218548497

Start: 1167463

End: 1168266

Strand: Direct

Name: otsB [H]

Synonym: EFER_1126

Alternate gene names: 218548497

Gene position: 1167463-1168266 (Clockwise)

Preceding gene: 218548496

Following gene: 218548498

Centisome position: 25.44

GC content: 51.49

Gene sequence:

>804_bases
GTGACCAAACCGTTAACCGAAGCCCCTGAACTTTCCGCTAAATACGCCTGGTTTTTTGATCTTGATGGCACGCTGGCTGA
GATCAAACCGCATCCCGACCAGGTGGCGATTCCTGACGCGATTCTGCAAGGTCTTCAGCAACTGGCCGTCCATAGCGACG
GTGCGCTGGCATTGATTTCAGGGCGCTCAATGGTTGAGCTTGATACGCTGGCTAAACCATATCGTTTTCCCCTTGCCGGA
GTGCATGGAGCAGAACGCCGTGACATCAATGGTAAAACGCATATTGTGCGTCTGCCTGACGCCATGGTCCGGGATATCAG
CGTGCAGCTGCATACGACGCTGGCAGGCTTAACCGGAGTGGAGATTGAAGAAAAAGGGATGGCGTTTGCCCTGCATTACC
GCCAGGCACCGCAGCATGAAGCGCTGTTATTTACTCTGGCGCAGCGTATTACACAAATCTGGCCGCAAATGGCGCTTCAG
CAAGGCAAATGTGTGGTTGAAATTAAACCACGTGGCACCTGTAAAGGTGATGCCATTGCAGAATTTATGCAGGAGACCCC
GTTTGTTGGAAGGATACCGATTTTTTGGGGGGATGATTTAACGGATGAGTCCGGCTTTGCTGTCGTTAATCAGGCGGGAG
GCATATCGGTGAAAATAGGGGCAGGTGAGACCCAGGCGAAATGGCGGCTGGCGGGCGTGCCAGATGTCTGGCGCTGGCTT
GCAGTAATAACCAACTCATTACAAGAACAAAAATTAGAAGAGAACAGGAGTGATGATTATGAGTCGTTTAGTCGTAGTAT
CTAA

Upstream 100 bases:

>100_bases
TCATCTTATGCCAATGGCGAGCCTCTTCTCACTGTCTATACTTACATGTCTGTAAGGCGCGTTCTGCGCGAAATAAAACA
AGATAAAGGAGGAGGAACGG

Downstream 100 bases:

>100_bases
CCGTATTGCCCCACCTGATGAGCATGCCGCCAGTGCTGGTGGTCTTGCCGTTGGTATACTGGGCGCATTGAAGGCTGCAG
GGGGGTTATGGTTTGGCTGG

Product: trehalose-6-phosphate phosphatase

Products: NA

Alternate protein names: TPP; Osmoregulatory trehalose synthesis protein B; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTKPLTEAPELSAKYAWFFDLDGTLAEIKPHPDQVAIPDAILQGLQQLAVHSDGALALISGRSMVELDTLAKPYRFPLAG
VHGAERRDINGKTHIVRLPDAMVRDISVQLHTTLAGLTGVEIEEKGMAFALHYRQAPQHEALLFTLAQRITQIWPQMALQ
QGKCVVEIKPRGTCKGDAIAEFMQETPFVGRIPIFWGDDLTDESGFAVVNQAGGISVKIGAGETQAKWRLAGVPDVWRWL
AVITNSLQEQKLEENRSDDYESFSRSI

Sequences:

>Translated_267_residues
MTKPLTEAPELSAKYAWFFDLDGTLAEIKPHPDQVAIPDAILQGLQQLAVHSDGALALISGRSMVELDTLAKPYRFPLAG
VHGAERRDINGKTHIVRLPDAMVRDISVQLHTTLAGLTGVEIEEKGMAFALHYRQAPQHEALLFTLAQRITQIWPQMALQ
QGKCVVEIKPRGTCKGDAIAEFMQETPFVGRIPIFWGDDLTDESGFAVVNQAGGISVKIGAGETQAKWRLAGVPDVWRWL
AVITNSLQEQKLEENRSDDYESFSRSI
>Mature_266_residues
TKPLTEAPELSAKYAWFFDLDGTLAEIKPHPDQVAIPDAILQGLQQLAVHSDGALALISGRSMVELDTLAKPYRFPLAGV
HGAERRDINGKTHIVRLPDAMVRDISVQLHTTLAGLTGVEIEEKGMAFALHYRQAPQHEALLFTLAQRITQIWPQMALQQ
GKCVVEIKPRGTCKGDAIAEFMQETPFVGRIPIFWGDDLTDESGFAVVNQAGGISVKIGAGETQAKWRLAGVPDVWRWLA
VITNSLQEQKLEENRSDDYESFSRSI

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=267, Percent_Identity=83.1460674157303, Blast_Score=458, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19920676, Length=254, Percent_Identity=26.7716535433071, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221473368, Length=248, Percent_Identity=27.8225806451613, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI20129307, Length=248, Percent_Identity=27.8225806451613, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24582490, Length=248, Percent_Identity=27.8225806451613, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 29512; Mature: 29381

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPLTEAPELSAKYAWFFDLDGTLAEIKPHPDQVAIPDAILQGLQQLAVHSDGALALIS
CCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCEECHHHHHHHHHHHHHCCCCCEEEEC
GRSMVELDTLAKPYRFPLAGVHGAERRDINGKTHIVRLPDAMVRDISVQLHTTLAGLTGV
CCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHEEEEHHHHCCCCC
EIEEKGMAFALHYRQAPQHEALLFTLAQRITQIWPQMALQQGKCVVEIKPRGTCKGDAIA
EEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHH
EFMQETPFVGRIPIFWGDDLTDESGFAVVNQAGGISVKIGAGETQAKWRLAGVPDVWRWL
HHHHHCCCCEEEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCEEEECCCHHHHHHH
AVITNSLQEQKLEENRSDDYESFSRSI
HHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure 
TKPLTEAPELSAKYAWFFDLDGTLAEIKPHPDQVAIPDAILQGLQQLAVHSDGALALIS
CCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCEECHHHHHHHHHHHHHCCCCCEEEEC
GRSMVELDTLAKPYRFPLAGVHGAERRDINGKTHIVRLPDAMVRDISVQLHTTLAGLTGV
CCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHEEEEHHHHCCCCC
EIEEKGMAFALHYRQAPQHEALLFTLAQRITQIWPQMALQQGKCVVEIKPRGTCKGDAIA
EEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHH
EFMQETPFVGRIPIFWGDDLTDESGFAVVNQAGGISVKIGAGETQAKWRLAGVPDVWRWL
HHHHHCCCCEEEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCEEEECCCHHHHHHH
AVITNSLQEQKLEENRSDDYESFSRSI
HHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045430; 9097040; 9278503 [H]