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The map label for this gene is hyaF [H]

Identifier: 218548487

GI number: 218548487

Start: 1157745

End: 1158659

Strand: Direct

Name: hyaF [H]

Synonym: EFER_1115

Alternate gene names: 218548487

Gene position: 1157745-1158659 (Clockwise)

Preceding gene: 218548486

Following gene: 218548488

Centisome position: 25.23

GC content: 54.54

Gene sequence:

>915_bases
GTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAACCGCAACAGGAGCGTGCCTCATGAGCGAAACTTTTTTCCA
TCTACTGGGACCCGGAACGCAGCCTGATGATGACAGTTTTAGCATGAATCCACTGCCGATTACCTGTCAGGTGAATGATG
AACCGAGTATGGCGGCCCTGGAGCAATGTGCTCATAGCCCGCAGGTGATTGCGCTATTAAACGAGTTACAACATCAACTA
AGCGAACGCCAGCCGCCGCTGGGTGAGGTGCTGGCAGTCGATCTGTTCAATCTCAACGCCGACGATCGTCACTTTATCAA
TACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGTTGATGGTAGTGAAAGTGAAATTCAGGAGGCGATCT
TCTGCGGATTATGGCGGGTACGCAGACGTCGCGGTGAACAGTTGCTGGAGGACAAACTGGAGGCAGGCTGCGCGCCGCTG
GCGTTGTGGCAGGCGGCAACGCAAAACGTCTTGCCGACAGACTCGTTGCTACCGCCGCCCATCGATGGCCTGATGAATGG
CCTACCGCTGGCGCATGAATTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTAC
CCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGTCGGGAAATATTCAGATTCGCACCATTGGCTATGGC
GAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGCACGGACACCTTAAAAGGCCCATTACTTGA
AAGTTATGAAATCTGTCCAATACCGGAAGTGGTACTGGCGGCACCAGAAGATTTGGCGGATTCCGCGCAGCGCCTGGCAG
AAGTAAGCCAGTGGTTACAGGAGTGTGTCGGTTAG

Upstream 100 bases:

>100_bases
AAAGCGAAGCCATCGGCGATCGCTTTGGCGTATTTCGTTTTCCCGCCACACTGGTGTTTACCGGTGGTAGCTATCGCGGC
GTGCTGAATGGTATTCACCC

Downstream 100 bases:

>100_bases
ATTTATACGCGGCTTACTTTTTGGTAAGTAGGCGATATCACGTCTGGCATGTAAGATAGACCCAGCGATTTATTTTCACT
GAGGACAAGCGCGATGAATA

Product: hydrogenase-1 nickel incorporation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MGGTDKPDARACRTATGACLMSETFFHLLGPGTQPDDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQL
SERQPPLGEVLAVDLFNLNADDRHFINTLLGEGEVSVRIQQVDGSESEIQEAIFCGLWRVRRRRGEQLLEDKLEAGCAPL
ALWQAATQNVLPTDSLLPPPIDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGSGNIQIRTIGYG
ESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLADSAQRLAEVSQWLQECVG

Sequences:

>Translated_304_residues
MGGTDKPDARACRTATGACLMSETFFHLLGPGTQPDDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQL
SERQPPLGEVLAVDLFNLNADDRHFINTLLGEGEVSVRIQQVDGSESEIQEAIFCGLWRVRRRRGEQLLEDKLEAGCAPL
ALWQAATQNVLPTDSLLPPPIDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGSGNIQIRTIGYG
ESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLADSAQRLAEVSQWLQECVG
>Mature_303_residues
GGTDKPDARACRTATGACLMSETFFHLLGPGTQPDDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQLS
ERQPPLGEVLAVDLFNLNADDRHFINTLLGEGEVSVRIQQVDGSESEIQEAIFCGLWRVRRRRGEQLLEDKLEAGCAPLA
LWQAATQNVLPTDSLLPPPIDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGSGNIQIRTIGYGE
SYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLADSAQRLAEVSQWLQECVG

Specific function: Not known. Could enhance the incorporation of nickel to the hydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupH/hyaF family [H]

Homologues:

Organism=Escherichia coli, GI1787211, Length=281, Percent_Identity=96.085409252669, Blast_Score=551, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006894 [H]

Pfam domain/function: PF04809 HupH_C [H]

EC number: NA

Molecular weight: Translated: 33305; Mature: 33174

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGTDKPDARACRTATGACLMSETFFHLLGPGTQPDDDSFSMNPLPITCQVNDEPSMAAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHH
EQCAHSPQVIALLNELQHQLSERQPPLGEVLAVDLFNLNADDRHFINTLLGEGEVSVRIQ
HHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHEEHEECCCCCHHHHHHHHHCCCCEEEEEE
QVDGSESEIQEAIFCGLWRVRRRRGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCC
IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGSGNIQIRTIGYG
HHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECC
ESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLADSAQRLAEVSQWLQ
CHHHHHHHHHHEEEEEECHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHH
ECVG
HHCC
>Mature Secondary Structure 
GGTDKPDARACRTATGACLMSETFFHLLGPGTQPDDDSFSMNPLPITCQVNDEPSMAAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHH
EQCAHSPQVIALLNELQHQLSERQPPLGEVLAVDLFNLNADDRHFINTLLGEGEVSVRIQ
HHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHEEHEECCCCCHHHHHHHHHCCCCEEEEEE
QVDGSESEIQEAIFCGLWRVRRRRGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCC
IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGSGNIQIRTIGYG
HHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECC
ESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLADSAQRLAEVSQWLQ
CHHHHHHHHHHEEEEEECHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHH
ECVG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]