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The map label for this gene is hyaD [H]

Identifier: 218548485

GI number: 218548485

Start: 1156826

End: 1157413

Strand: Direct

Name: hyaD [H]

Synonym: EFER_1113

Alternate gene names: 218548485

Gene position: 1156826-1157413 (Clockwise)

Preceding gene: 218548484

Following gene: 218548486

Centisome position: 25.21

GC content: 58.67

Gene sequence:

>588_bases
ATGAGTGAGCAACGCGTGGTGGTCATGGGACTGGGCAATCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGA
ACGGTTGTATGCCCATTACCACTGGCCCGATGAAGTCGAGATTGTTGATGGCGGCACTCAGGGACTGAACTTGCTGGGGT
ATGTCGAAAGCGCCAGCCACTTGCTGATTCTCGACGCCATTGATTACGGGCTGGAACCCGGAGCGCTACGAACGTATGCC
GGAGAACGCATTCCGGCCTATCTCAGCGCGAAGAAAATGAGCCTGCACCAGAACAGTTTCTCCGAAGTACTGGCGCTGGC
AGATATCCGCGGAAATCTGCCAGCACATATTGCCCTCGTCGGCCTGCAACCCGCGATGCTCGACGACTACGGCGGTAGCC
TGAGCGAACTGGCTCGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGTACAACTGGCTGTGTGGGGAATTGTGCCG
CAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAACTACGAAGGCGTTCGCTTGCGCCAGTA
CCGGATGACGCTGGAGGAGCAGGAATGA

Upstream 100 bases:

>100_bases
CGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTTAACGGCTACCGTAGCCACAAATTTGGCAAA
ATGAGTAACAAGGAGCGTTC

Downstream 100 bases:

>100_bases
GCAACGACACGCCATTTGATGCGTTGTGGCAACGTATGCTGGCGCGCGGCTGGACGCCGATCAGTGAATCCCGTCTTGAC
GACTGGCTTACGCAAGCGCC

Product: hydrogenase 1 maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGALRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGNLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAVWGIVP
QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQE

Sequences:

>Translated_195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGALRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGNLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAVWGIVP
QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQE
>Mature_194_residues
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGALRTYAG
ERIPAYLSAKKMSLHQNSFSEVLALADIRGNLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAVWGIVPQ
PANESRCLNYDCLSMENYEGVRLRQYRMTLEEQE

Specific function: Protease involved in the C-terminal processing of hyaB, the large subunit of hydrogenase 1 (Potential) [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=194, Percent_Identity=96.3917525773196, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789367, Length=157, Percent_Identity=38.8535031847134, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 21559; Mature: 21428

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDGGTQGLNLLGYVESASH
CCCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCE
LLILDAIDYGLEPGALRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGNLPAHIALV
EEEEEEHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAVWGIVPQPANESRCLNYDCLSMENYE
ECCCHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCC
GVRLRQYRMTLEEQE
CCEEEEEEEHHCCCC
>Mature Secondary Structure 
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDGGTQGLNLLGYVESASH
CCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCE
LLILDAIDYGLEPGALRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGNLPAHIALV
EEEEEEHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAVWGIVPQPANESRCLNYDCLSMENYE
ECCCHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCC
GVRLRQYRMTLEEQE
CCEEEEEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]