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The map label for this gene is yahI [H]

Identifier: 218547833

GI number: 218547833

Start: 432708

End: 433658

Strand: Direct

Name: yahI [H]

Synonym: EFER_0421

Alternate gene names: 218547833

Gene position: 432708-433658 (Clockwise)

Preceding gene: 218547832

Following gene: 218547834

Centisome position: 9.43

GC content: 56.68

Gene sequence:

>951_bases
ATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGC
GGAGGCGGTGAAAGCCGTGGCCGAGACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACG
GGCCGCAGGTTGGGCTGGATCTACGCCGTGCGGAGATTGCCCACGAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAAC
TGTGTGGCAGATACGCAAGGCGGCATTGGCTACTTGATCCAACAGGCGCTGAATAACCGACTGGCGCGTCACGGCGAGAA
AAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAATGATCCGGGTTTTGCTCACCCCACCAAACCTATCGGTG
CATTCTTTAGTGAAAGCCAGCGTGACGAATTACAAAAGGCAAACCCTGACTGGCGTTTTGTTGAAGATGCCGGGCGGGGC
TATCGCCGCGTGGTCGCCTCGCCGGAACCGAAACGTATTGTCGAAGCACCTGCCATTAAGGCCCTGATCCAGCAAGGCTT
TGTGGTGATTGGCGCGGGCGGTGGCGGAATTCCGGTGGTGCGCAGCGAAGCGGGGGATTACCAAAGCGTGGACGCGGTTA
TCGACAAAGATCTCTCCACCGCGCTGCTGGCCCGCGAAATTCACGCCGACATTCTGGTGATCACCACTGGTGTCGAAAAA
GTCTGTATTCATTTTGGCAAACCGCAGCAGCAGGCGCTCGATCGGGTGGATGTTGCCACCATGACCCGCTATATGCAGGA
AGGGCATTTCCCGCCTGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGACATTTTTAGAACAGGGCGGCAAAGAAGTGA
TTATCACCACGCCGGAATGCCTGCCCGCGGCGCTGCGTGGCAAGACGGGCACCCATATTATTAAAACGTAA

Upstream 100 bases:

>100_bases
TCTGACCTGCAAAGTGCACTTTACGATCCCTTCTCCCTTCTGGGGAGAGGGGCAACCTCACTTTCATAACCTCATCACCC
TCTGTAGCGGACATAACAAC

Downstream 100 bases:

>100_bases
GGACGTAAGATGAAAGAAAACAATAGCCGTCGTGAGTTTTTGAGCCAGAGCGGTAAGATGGTCACCGCCGCCGCGCTGTT
TGGTACCTCTGTGCCGCTCG

Product: carbamate kinase

Products: ADP; carbamoyl phosphate

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVAETVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRSEAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
VCIHFGKPQQQALDRVDVATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGKTGTHIIKT

Sequences:

>Translated_316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVAETVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRSEAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
VCIHFGKPQQQALDRVDVATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGKTGTHIIKT
>Mature_316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVAETVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRSEAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
VCIHFGKPQQQALDRVDVATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGKTGTHIIKT

Specific function: Unknown

COG id: COG0549

COG function: function code E; Carbamate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbamate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786516, Length=316, Percent_Identity=97.4683544303797, Blast_Score=595, Evalue=1e-172,
Organism=Escherichia coli, GI1789238, Length=318, Percent_Identity=46.2264150943396, Blast_Score=262, Evalue=2e-71,
Organism=Escherichia coli, GI1786732, Length=313, Percent_Identity=38.0191693290735, Blast_Score=174, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR003964 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: 2.7.2.2

Molecular weight: Translated: 33998; Mature: 33998

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVAETVLEMLASDYDIVLTHGNGPQVGLD
CCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
LRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDK
HHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC
NDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRGYRRVVASPEPKRIVEAPAIK
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCEEECCCCCCHHHHHHHHHH
ALIQQGFVVIGAGGGGIPVVRSEAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
HHHHCCCEEEECCCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEEECCHHH
VCIHFGKPQQQALDRVDVATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPEC
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
LPAALRGKTGTHIIKT
HHHHHCCCCCCEEECC
>Mature Secondary Structure
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVAETVLEMLASDYDIVLTHGNGPQVGLD
CCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
LRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDK
HHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC
NDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRGYRRVVASPEPKRIVEAPAIK
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCEEECCCCCCHHHHHHHHHH
ALIQQGFVVIGAGGGGIPVVRSEAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
HHHHCCCEEEECCCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEEECCHHH
VCIHFGKPQQQALDRVDVATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPEC
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
LPAALRGKTGTHIIKT
HHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; NH3; CO2

Specific reaction: ATP + NH3 + CO2 = ADP + carbamoyl phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]