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The map label for this gene is rpoS [H]

Identifier: 218547743

GI number: 218547743

Start: 338431

End: 339306

Strand: Direct

Name: rpoS [H]

Synonym: EFER_0328

Alternate gene names: 218547743

Gene position: 338431-339306 (Clockwise)

Preceding gene: 218547742

Following gene: 218547745

Centisome position: 7.38

GC content: 53.77

Gene sequence:

>876_bases
TTGGCTGAAGAGGAGCTGTTATCGCAAGGGGCCACACAGCGTGTGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGG
TTATTCGCCACTGTTAACGGCCGAAGAAGAAGTTTATTTCGCGCGTCGCGCACTGCGTGGAGATGTCGCTTCACGTCGCC
GGATGATTGAAAGTAACCTGCGTCTGGTCGTGAAGATTGCCCGCCGTTATGGCAATCGTGGTCTGGCGTTATTGGATCTG
ATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTTGAGAAGTTTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGC
AACCTGGTGGATTCGCCAGACGATCGAGCGGGCGATTATGAACCAAACCCGTACTATTCGTTTGCCGATTCACATCGTAA
AGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCATAAGCTGGACCACGAACCGAGCGCGGAAGAGATCGCA
GAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTCGTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCT
GGGTGGAGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGATGAAAAAGAAAACGGCCCGGAAGATACCACGCAAGATG
ACGATATGAAACAAAGCATCGTCAAATGGCTGTTCGAACTGAACGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGT
TTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTGAAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCA
GGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Upstream 100 bases:

>100_bases
GAAAGTTCATGATTTAAATGAAGACGCGGAATTTGATGAGAACTGAGTTGAGGTTTTTGACGAGAAAGCCTTAGTTGAAG
AGGAACCCAGTGATAACGAT

Downstream 100 bases:

>100_bases
GTAAGCATCTGTCAGAAAGGCCAGTCTCAAGCGAGGCTGGCCTTTTTCTTTTATTTGGGGAGTCGGTGTGTTTTGTCGCC
TGGATAAGACACGTCAGCGT

Product: RNA polymerase, sigma S (sigma 38) factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDL
IEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIA
EQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFG
LLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE

Sequences:

>Translated_291_residues
MAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDL
IEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIA
EQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFG
LLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE
>Mature_290_residues
AEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLI
EEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAE
QLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGL
LGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=291, Percent_Identity=99.6563573883162, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1789448, Length=247, Percent_Identity=42.1052631578947, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1789871, Length=268, Percent_Identity=26.865671641791, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33557; Mature: 33426

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNL
CCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIM
HHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHH
NQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERI
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
TSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFG
HHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
LLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE
HHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
>Mature Secondary Structure 
AEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNL
CHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIM
HHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHH
NQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERI
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
TSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFG
HHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
LLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE
HHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]