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The map label for this gene is rumA [H]

Identifier: 218547699

GI number: 218547699

Start: 296588

End: 297886

Strand: Direct

Name: rumA [H]

Synonym: EFER_0279

Alternate gene names: 218547699

Gene position: 296588-297886 (Clockwise)

Preceding gene: 218547697

Following gene: 218547700

Centisome position: 6.46

GC content: 51.66

Gene sequence:

>1299_bases
ATGGCGCAATTCTACTCTGCAAAACGACGCGTGACGACGCGTCAGATCATAACCGTTACAGCCAACGACCTCGACGCTTT
TGGTCAGGGTGTCGCTCGTCATAACGGAAAAGCGCTGTTTATTCCCGGCTTATTGCCGCAAGAAAGCGCGGAAGTCTCTG
TCACCGAAGATAAAAGGCAATTTGCCCGGGCTAAAGTTGTTCGTCGTTTGAACGATAGTCCCCAGCGTGAAGTTCCTCGC
TGCCCACATTTTGGCGTTTGCGGTGGCTGCCAGCAACAGCATGCCAGCGTCGAACTCCAGCAGCAAAGCAAAAGTGCGGC
GTTGGCGAGATTAATGAAACACGATGTTGCAGATATTATCGCTGATGCGCCGTGGGGATATCGCCGCCGTGCGCGGTTAA
GCCTGAATTATCTGGCCAAAACGCAACAATTACAGATGGGGTTTCGCAAGGCTGCCTCCAGTGACATTGTCGATGTGAAG
CAATGCCCTATTCTGGTGCCCCAAATTGAAGCATTACTGCCAAAGGTCAGAGCATGTCTGATGAGTTTGCAGACAGTGCG
CCATCTCGGACACGTCGAGCTGGTGCAAGCGACCAGCGGGACGTTAATGATTTTGCGCCACACTGCGCCGCTAAGTTCTG
CCGACAGAGAAAAACTGGAACGCTTTTCGCATTGTGAAAGACTTTCTCTGTTCCTGGCGCCCGATAGTGAGATACTCGAA
CCCCTGGTCGGTGAGACGCCCTGGTATGATTCCAACGGTCTACGGTTGATGTTTAGCCCACGCGATTTCATCCAGGTGAA
TGACGGCGTAAACCAGAAAATGGTTGCACAAGCCATTGAATGGCTGGATATCCAGCCACAGGATCGCGTGCTGGATCTGT
TCTGTGGAATGGGGAACTTTACCCTGGCGTTGGCAACACGCGCAGCCAGTGTGGTAGGTGTAGAAGGTGTTCCTGCTTTG
GTCGAAAAAGGGCGAGAAAATGCACGTGAAAATGGCTTAGCTAATGTCACGTTTTATCACGAAAATCTTGAAGACGATGT
TACGAAGCAGCCCTGGGCGAAAAACGGCTTTGATAAAGTCCTTCTTGACCCGGCGCGGGCAGGTGCAGCAGGGGTAATGC
AGCAAATTATAAAGCTGGAACCAATTCGTGTTGTTTATGTATCCTGTAACCCTGCGACGCTGGCTCGGGATAGCGAAGCG
TTAATAAATGCAGGATATACCATTGCGCGACTGGCGATGCTGGATATGTTCCCACACACGGGGCATCTGGAGTCGATGGT
TCTTTTTGAGCGCGTGTAA

Upstream 100 bases:

>100_bases
AGGATCAGTATCATCATGCGTGCGCGCAGGCTGTAGTTGGTCATGGAGTTCCGTTATGGGACAATTAAGTTCACACTGTT
AAATTGAGAAAGTCTCGCCA

Downstream 100 bases:

>100_bases
TAATTGCAGCTTGTCGTTTACGACAGGCTTTGTCCCTAAAGGAGAGGACGATGGTTGCGGTAAGAAGTGCACATATCAAT
AAAGCTGGTGAATTTGATCC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MAQFYSAKRRVTTRQIITVTANDLDAFGQGVARHNGKALFIPGLLPQESAEVSVTEDKRQFARAKVVRRLNDSPQREVPR
CPHFGVCGGCQQQHASVELQQQSKSAALARLMKHDVADIIADAPWGYRRRARLSLNYLAKTQQLQMGFRKAASSDIVDVK
QCPILVPQIEALLPKVRACLMSLQTVRHLGHVELVQATSGTLMILRHTAPLSSADREKLERFSHCERLSLFLAPDSEILE
PLVGETPWYDSNGLRLMFSPRDFIQVNDGVNQKMVAQAIEWLDIQPQDRVLDLFCGMGNFTLALATRAASVVGVEGVPAL
VEKGRENARENGLANVTFYHENLEDDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRVVYVSCNPATLARDSEA
LINAGYTIARLAMLDMFPHTGHLESMVLFERV

Sequences:

>Translated_432_residues
MAQFYSAKRRVTTRQIITVTANDLDAFGQGVARHNGKALFIPGLLPQESAEVSVTEDKRQFARAKVVRRLNDSPQREVPR
CPHFGVCGGCQQQHASVELQQQSKSAALARLMKHDVADIIADAPWGYRRRARLSLNYLAKTQQLQMGFRKAASSDIVDVK
QCPILVPQIEALLPKVRACLMSLQTVRHLGHVELVQATSGTLMILRHTAPLSSADREKLERFSHCERLSLFLAPDSEILE
PLVGETPWYDSNGLRLMFSPRDFIQVNDGVNQKMVAQAIEWLDIQPQDRVLDLFCGMGNFTLALATRAASVVGVEGVPAL
VEKGRENARENGLANVTFYHENLEDDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRVVYVSCNPATLARDSEA
LINAGYTIARLAMLDMFPHTGHLESMVLFERV
>Mature_431_residues
AQFYSAKRRVTTRQIITVTANDLDAFGQGVARHNGKALFIPGLLPQESAEVSVTEDKRQFARAKVVRRLNDSPQREVPRC
PHFGVCGGCQQQHASVELQQQSKSAALARLMKHDVADIIADAPWGYRRRARLSLNYLAKTQQLQMGFRKAASSDIVDVKQ
CPILVPQIEALLPKVRACLMSLQTVRHLGHVELVQATSGTLMILRHTAPLSSADREKLERFSHCERLSLFLAPDSEILEP
LVGETPWYDSNGLRLMFSPRDFIQVNDGVNQKMVAQAIEWLDIQPQDRVLDLFCGMGNFTLALATRAASVVGVEGVPALV
EKGRENARENGLANVTFYHENLEDDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRVVYVSCNPATLARDSEAL
INAGYTIARLAMLDMFPHTGHLESMVLFERV

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=194, Percent_Identity=35.0515463917526, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI51173878, Length=194, Percent_Identity=35.0515463917526, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI269784680, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI269784676, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI20149688, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI269784678, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789148, Length=432, Percent_Identity=88.4259259259259, Blast_Score=809, Evalue=0.0,
Organism=Escherichia coli, GI1787083, Length=383, Percent_Identity=26.8929503916449, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790403, Length=178, Percent_Identity=30.8988764044944, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24666579, Length=213, Percent_Identity=31.924882629108, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 48000; Mature: 47869

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFYSAKRRVTTRQIITVTANDLDAFGQGVARHNGKALFIPGLLPQESAEVSVTEDKRQ
CCCHHHHHHHCCEEEEEEEECCCHHHHHCHHHHCCCCEEEECCCCCCCCCCEEECHHHHH
FARAKVVRRLNDSPQREVPRCPHFGVCGGCQQQHASVELQQQSKSAALARLMKHDVADII
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ADAPWGYRRRARLSLNYLAKTQQLQMGFRKAASSDIVDVKQCPILVPQIEALLPKVRACL
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEHHHHHHHHHHHHHHH
MSLQTVRHLGHVELVQATSGTLMILRHTAPLSSADREKLERFSHCERLSLFLAPDSEILE
HHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHH
PLVGETPWYDSNGLRLMFSPRDFIQVNDGVNQKMVAQAIEWLDIQPQDRVLDLFCGMGNF
HHHCCCCCCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCH
TLALATRAASVVGVEGVPALVEKGRENARENGLANVTFYHENLEDDVTKQPWAKNGFDKV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHCCCCCCCCCCCCE
LLDPARAGAAGVMQQIIKLEPIRVVYVSCNPATLARDSEALINAGYTIARLAMLDMFPHT
EECCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHCCCCHHHHHCCHHHHHHHHHHHCCCC
GHLESMVLFERV
CCHHHHHHHHCC
>Mature Secondary Structure 
AQFYSAKRRVTTRQIITVTANDLDAFGQGVARHNGKALFIPGLLPQESAEVSVTEDKRQ
CCHHHHHHHCCEEEEEEEECCCHHHHHCHHHHCCCCEEEECCCCCCCCCCEEECHHHHH
FARAKVVRRLNDSPQREVPRCPHFGVCGGCQQQHASVELQQQSKSAALARLMKHDVADII
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ADAPWGYRRRARLSLNYLAKTQQLQMGFRKAASSDIVDVKQCPILVPQIEALLPKVRACL
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEHHHHHHHHHHHHHHH
MSLQTVRHLGHVELVQATSGTLMILRHTAPLSSADREKLERFSHCERLSLFLAPDSEILE
HHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHH
PLVGETPWYDSNGLRLMFSPRDFIQVNDGVNQKMVAQAIEWLDIQPQDRVLDLFCGMGNF
HHHCCCCCCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCH
TLALATRAASVVGVEGVPALVEKGRENARENGLANVTFYHENLEDDVTKQPWAKNGFDKV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHCCCCCCCCCCCCE
LLDPARAGAAGVMQQIIKLEPIRVVYVSCNPATLARDSEALINAGYTIARLAMLDMFPHT
EECCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHCCCCHHHHHCCHHHHHHHHHHHCCCC
GHLESMVLFERV
CCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]