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The map label for this gene is garK [H]

Identifier: 218547697

GI number: 218547697

Start: 292506

End: 293660

Strand: Direct

Name: garK [H]

Synonym: EFER_0277

Alternate gene names: 218547697

Gene position: 292506-293660 (Clockwise)

Preceding gene: 218547696

Following gene: 218547699

Centisome position: 6.37

GC content: 51.52

Gene sequence:

>1155_bases
ATGAAAATCGTAATCGCACCGGATTCATATAAAGAAAGCCTGAACGCTTTTGCAGTGGCGACCGCCATTGAGCAGGGTTT
CCGGGAAATCTGGCCTGATGCGGATTACGTAAAACTGCCAGTAGCAGATGGTGGTGAAGGTACTGTCGAGGCGATGGTTG
AGGCGACAAGCGGTCATATTGTTCATGTTGATGTTACCGGACCGCTTGGGCAACAAGTGTCGGCATTTTATGGCCTTTCT
GGTGATGAACGTACGGCGTTTATTGAAATGGCAGCAGCCAGCGGCCTGGAACTGGTGCCCGTCAATAAACGTAATCCGCT
GATAACCACCTCCTGGGGAACAGGAGAGCTCATTCGCCATGCGCTTGATGCGGGTGTTGAGCATATCATTATTGGAATTG
GTGGCAGTGCAACCAATGATGGCGGTGCCGGAATGGTACAAGCACTGGGAGCGAAACTGCTGGATGCGCAAGGACAACAA
ATTGCGCAAGGTGGTGCAGCACTTGAATTGCTGGCAAAAATTGATATCAGTCAGTTGGATCCCCGGTTGGCAAGTTGCCG
TATTGAAGTGGCTTGTGACGTCACCAATCCTTTAACGGGAGAAGAGGGCGCATCAGTGATTTTTGGGCCGCAGAAAGGCG
CGACACCGAAAATGATTGCGCGACTGGATAATGCACTTAGCCATTATGCACAGATCATCGCAAGTGATCTGGGGCTTGAA
GTGTCAGAACTTGCAGGCGGTGGTGCTGCGGGCGGCATGGGCGTGGCGCTATATGCGTTTTGTGGTGCTCAGTTACGCCG
TGGCATTGAAATTGTGACCGACGCGTTAAACCTCGATGAGTGTGTGGCCGATGCAGATCTGGTGATTACCGGAGAAGGGC
GGATCGACAGTCAAACCATTCACGGTAAAGTACCTGTTGGTGTGGCGAAGATTGCCAAGCGCTACCATAAGCCGGTGATC
GGAATTGCAGGCAGCCTGACAGCGGATGTGGGGGTTGTTCACCAGCATGGGCTGGATGCTGTGTTTAGTGTGATTTACAG
TGTGTGTTCACTGGACGATGCACTGGAAAATGCTGCTGAAAATGTGCGCCTGACCGCGCGTAATATTGCCGCGACAATTA
AGATTGGCCAGACTGTTAATTCAGCACCATATTAA

Upstream 100 bases:

>100_bases
ATGGTGCGTTAATCATGATGCCGGTTCCCGAGGAAATCGGCATTATTCACCCACAAATTGAATGTGTTATTAACATAATT
GGCATGAATAAGGACGGCTA

Downstream 100 bases:

>100_bases
AAAATGGATATTGGATTAATTTTAATTCATTGAATTTAAAATATATTATTAGCCGAATGGAGCGTTTGCGCTGCATCCGG
CTAATAACATGTCACTCAGT

Product: glycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKIVIAPDSYKESLNAFAVATAIEQGFREIWPDADYVKLPVADGGEGTVEAMVEATSGHIVHVDVTGPLGQQVSAFYGLS
GDERTAFIEMAAASGLELVPVNKRNPLITTSWGTGELIRHALDAGVEHIIIGIGGSATNDGGAGMVQALGAKLLDAQGQQ
IAQGGAALELLAKIDISQLDPRLASCRIEVACDVTNPLTGEEGASVIFGPQKGATPKMIARLDNALSHYAQIIASDLGLE
VSELAGGGAAGGMGVALYAFCGAQLRRGIEIVTDALNLDECVADADLVITGEGRIDSQTIHGKVPVGVAKIAKRYHKPVI
GIAGSLTADVGVVHQHGLDAVFSVIYSVCSLDDALENAAENVRLTARNIAATIKIGQTVNSAPY

Sequences:

>Translated_384_residues
MKIVIAPDSYKESLNAFAVATAIEQGFREIWPDADYVKLPVADGGEGTVEAMVEATSGHIVHVDVTGPLGQQVSAFYGLS
GDERTAFIEMAAASGLELVPVNKRNPLITTSWGTGELIRHALDAGVEHIIIGIGGSATNDGGAGMVQALGAKLLDAQGQQ
IAQGGAALELLAKIDISQLDPRLASCRIEVACDVTNPLTGEEGASVIFGPQKGATPKMIARLDNALSHYAQIIASDLGLE
VSELAGGGAAGGMGVALYAFCGAQLRRGIEIVTDALNLDECVADADLVITGEGRIDSQTIHGKVPVGVAKIAKRYHKPVI
GIAGSLTADVGVVHQHGLDAVFSVIYSVCSLDDALENAAENVRLTARNIAATIKIGQTVNSAPY
>Mature_384_residues
MKIVIAPDSYKESLNAFAVATAIEQGFREIWPDADYVKLPVADGGEGTVEAMVEATSGHIVHVDVTGPLGQQVSAFYGLS
GDERTAFIEMAAASGLELVPVNKRNPLITTSWGTGELIRHALDAGVEHIIIGIGGSATNDGGAGMVQALGAKLLDAQGQQ
IAQGGAALELLAKIDISQLDPRLASCRIEVACDVTNPLTGEEGASVIFGPQKGATPKMIARLDNALSHYAQIIASDLGLE
VSELAGGGAAGGMGVALYAFCGAQLRRGIEIVTDALNLDECVADADLVITGEGRIDSQTIHGKVPVGVAKIAKRYHKPVI
GIAGSLTADVGVVHQHGLDAVFSVIYSVCSLDDALENAAENVRLTARNIAATIKIGQTVNSAPY

Specific function: Unknown

COG id: COG1929

COG function: function code G; Glycerate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycerate kinase type-1 family [H]

Homologues:

Organism=Escherichia coli, GI145693185, Length=376, Percent_Identity=69.4148936170213, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1786724, Length=381, Percent_Identity=58.005249343832, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018193
- InterPro:   IPR004381 [H]

Pfam domain/function: PF02595 Gly_kinase [H]

EC number: =2.7.1.31 [H]

Molecular weight: Translated: 39682; Mature: 39682

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVIAPDSYKESLNAFAVATAIEQGFREIWPDADYVKLPVADGGEGTVEAMVEATSGHI
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHEEEECCCCE
VHVDVTGPLGQQVSAFYGLSGDERTAFIEMAAASGLELVPVNKRNPLITTSWGTGELIRH
EEEEECCCCCHHHHHHHCCCCCCCEEEEEEHHCCCCEEEECCCCCCEEEECCCHHHHHHH
ALDAGVEHIIIGIGGSATNDGGAGMVQALGAKLLDAQGQQIAQGGAALELLAKIDISQLD
HHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCHHHCC
PRLASCRIEVACDVTNPLTGEEGASVIFGPQKGATPKMIARLDNALSHYAQIIASDLGLE
CHHHCEEEEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
VSELAGGGAAGGMGVALYAFCGAQLRRGIEIVTDALNLDECVADADLVITGEGRIDSQTI
HHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHCCCCEEEECCCCCCCCEE
HGKVPVGVAKIAKRYHKPVIGIAGSLTADVGVVHQHGLDAVFSVIYSVCSLDDALENAAE
CCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
NVRLTARNIAATIKIGQTVNSAPY
CEEEEEEHEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKIVIAPDSYKESLNAFAVATAIEQGFREIWPDADYVKLPVADGGEGTVEAMVEATSGHI
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHEEEECCCCE
VHVDVTGPLGQQVSAFYGLSGDERTAFIEMAAASGLELVPVNKRNPLITTSWGTGELIRH
EEEEECCCCCHHHHHHHCCCCCCCEEEEEEHHCCCCEEEECCCCCCEEEECCCHHHHHHH
ALDAGVEHIIIGIGGSATNDGGAGMVQALGAKLLDAQGQQIAQGGAALELLAKIDISQLD
HHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCHHHCC
PRLASCRIEVACDVTNPLTGEEGASVIFGPQKGATPKMIARLDNALSHYAQIIASDLGLE
CHHHCEEEEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
VSELAGGGAAGGMGVALYAFCGAQLRRGIEIVTDALNLDECVADADLVITGEGRIDSQTI
HHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHCCCCEEEECCCCCCCCEE
HGKVPVGVAKIAKRYHKPVIGIAGSLTADVGVVHQHGLDAVFSVIYSVCSLDDALENAAE
CCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
NVRLTARNIAATIKIGQTVNSAPY
CEEEEEEHEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1705543; 9278503 [H]