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The map label for this gene is yaeL [H]

Identifier: 218547631

GI number: 218547631

Start: 218031

End: 219383

Strand: Direct

Name: yaeL [H]

Synonym: EFER_0198

Alternate gene names: 218547631

Gene position: 218031-219383 (Clockwise)

Preceding gene: 218547630

Following gene: 218547632

Centisome position: 4.75

GC content: 49.37

Gene sequence:

>1353_bases
ATGCTGAGTGTTCTCTGGGATTTGGCTTCATTCATCGTTGCGCTGGGTGTACTTATCACGGTGCATGAATTTGGTCATTT
CTGGGTTGCCCGGCGTTGTGGTGTTCGCGTCGAGCGCTTTTCCATTGGCTTTGGCAAAGCTCTCTGGCGCCGAACCGATC
GGTTAGGTACTGAATATGTCGTCGCCATGATCCCCCTTGGCGGCTATGTCAAAATGCTTGACGAGCGTGCTGAACCAGTC
GTTCCTGAGTTGCGCCGCCATGCCTTTAATAATAAAACTGTCGGCCAACGAGCAGCGATTATTGCAGCTGGCCCGATAGC
AAACTTCCTTTTCGCAATCTTTGCTTACTGGCTGGTTTTTATTATCGGCGTACCAGGTGTTCGCCCGGTTGTTGGTGAAA
TATCAGCCAATTCGATAGCTGCACAGGCGCAAATTGCACCAGGCACGGAACTAAAAGCGGTTGATGGTATCGAAACCCCT
GATTGGGATTCCGTGCGTTTACAACTGGTCGACAAAATCGGGGATGAGAGTACAACTCTTAGCGTAGCGCCCTTTGGTAG
CAACCAGCGAAGAGATGTAAAACTGGATTTACGTCAATGGTCATTTGAGCCAGACAAAGAAGATCCTGTATCTTCGTTGG
GTATTCGTCCACGTGGACCGCAGATTGAACCAGTACTGGTTGTTATTCAGGCAAACTCAGCAGGTATGAAGGCAGGTTTG
CAAGCAGGCGACAGGATCGTTAAAGTCGACGGTCAACCGTTAACGCAGTGGGGAACGTTTGTTACTCTGGTGCGCGACAA
TCCTGGTAAACCGTTGGCATTGGAAATTGAGCGTCAGGGAAACCCTTTATCACTGACGTTGATCCCGGAGAGTAAACCGG
GTAAAGGCAAAGCGATTGGATTTGCAGGCATTGAGCCTAAACTCATTCCTTTGCCGGAAGAGTATAAAATTGTACGCCAG
TATGGGCCGTTCAACGCCATCCTCGAAGCTACGGATAAAACGTGGCAGTTGATGAAACTGACGGTCAGTATGCTGGGAAA
ATTGATCACCGGTGATGTTAATCTGAACAACCTCAGTGGGCCGATATCTATCGCCAAAGGGGCTGGGATGACAGCGGAAC
TTGGAATTGTTTATTACCTGCCGTTTCTTGCGCTCATCAGCGTGAACTTAGGGATAATCAATCTGTTTCCGTTGCCCGTG
CTAGATGGGGGGCATCTGCTGTTCCTTGCGATCGAAAAGATCAAGGGCGGCCCGGTATCCGAGCGAGTTCAAGACTTTAG
TTATCGCATTGGCTCGATTTTGCTGGTGCTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTGTAA

Upstream 100 bases:

>100_bases
ATGGTGGTATTCTGGATCGTATTGATAGCCTGACCGCTGCTGTGCCGGTCTTTGCTTGCCTGTTGTTACTGGTATTCAGG
ACGCTTTAACGGAAGGTATT

Downstream 100 bases:

>100_bases
GAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTTAGCAGCGCCA
CCGTATACGGTGCTGAAGGG

Product: zinc metallopeptidase RseP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLSVLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRLGTEYVVAMIPLGGYVKMLDERAEPV
VPELRRHAFNNKTVGQRAAIIAAGPIANFLFAIFAYWLVFIIGVPGVRPVVGEISANSIAAQAQIAPGTELKAVDGIETP
DWDSVRLQLVDKIGDESTTLSVAPFGSNQRRDVKLDLRQWSFEPDKEDPVSSLGIRPRGPQIEPVLVVIQANSAGMKAGL
QAGDRIVKVDGQPLTQWGTFVTLVRDNPGKPLALEIERQGNPLSLTLIPESKPGKGKAIGFAGIEPKLIPLPEEYKIVRQ
YGPFNAILEATDKTWQLMKLTVSMLGKLITGDVNLNNLSGPISIAKGAGMTAELGIVYYLPFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKIKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL

Sequences:

>Translated_450_residues
MLSVLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRLGTEYVVAMIPLGGYVKMLDERAEPV
VPELRRHAFNNKTVGQRAAIIAAGPIANFLFAIFAYWLVFIIGVPGVRPVVGEISANSIAAQAQIAPGTELKAVDGIETP
DWDSVRLQLVDKIGDESTTLSVAPFGSNQRRDVKLDLRQWSFEPDKEDPVSSLGIRPRGPQIEPVLVVIQANSAGMKAGL
QAGDRIVKVDGQPLTQWGTFVTLVRDNPGKPLALEIERQGNPLSLTLIPESKPGKGKAIGFAGIEPKLIPLPEEYKIVRQ
YGPFNAILEATDKTWQLMKLTVSMLGKLITGDVNLNNLSGPISIAKGAGMTAELGIVYYLPFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKIKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL
>Mature_450_residues
MLSVLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRLGTEYVVAMIPLGGYVKMLDERAEPV
VPELRRHAFNNKTVGQRAAIIAAGPIANFLFAIFAYWLVFIIGVPGVRPVVGEISANSIAAQAQIAPGTELKAVDGIETP
DWDSVRLQLVDKIGDESTTLSVAPFGSNQRRDVKLDLRQWSFEPDKEDPVSSLGIRPRGPQIEPVLVVIQANSAGMKAGL
QAGDRIVKVDGQPLTQWGTFVTLVRDNPGKPLALEIERQGNPLSLTLIPESKPGKGKAIGFAGIEPKLIPLPEEYKIVRQ
YGPFNAILEATDKTWQLMKLTVSMLGKLITGDVNLNNLSGPISIAKGAGMTAELGIVYYLPFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKIKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL

Specific function: Cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of rseA. Acts on degS-cleaved rseA to release the cytoplasmic domain of rseA. This provides the cell with sigma E (rpoE) activity through the proteolysis of rseA. In vitr

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=450, Percent_Identity=92.2222222222222, Blast_Score=824, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 49090; Mature: 49090

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50106 PDZ ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRLGTEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEE
VAMIPLGGYVKMLDERAEPVVPELRRHAFNNKTVGQRAAIIAAGPIANFLFAIFAYWLVF
EEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHH
IIGVPGVRPVVGEISANSIAAQAQIAPGTELKAVDGIETPDWDSVRLQLVDKIGDESTTL
HHCCCCCCCHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCEE
SVAPFGSNQRRDVKLDLRQWSFEPDKEDPVSSLGIRPRGPQIEPVLVVIQANSAGMKAGL
EEEECCCCCCCEEEEEHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEEEEECCCCCCHHHH
QAGDRIVKVDGQPLTQWGTFVTLVRDNPGKPLALEIERQGNPLSLTLIPESKPGKGKAIG
CCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEEE
FAGIEPKLIPLPEEYKIVRQYGPFNAILEATDKTWQLMKLTVSMLGKLITGDVNLNNLSG
ECCCCCCEECCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PISIAKGAGMTAELGIVYYLPFLALISVNLGIINLFPLPVLDGGHLLFLAIEKIKGGPVS
CEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHCCCCHH
ERVQDFSYRIGSILLVLLMGLALFNDFSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSVLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRLGTEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEE
VAMIPLGGYVKMLDERAEPVVPELRRHAFNNKTVGQRAAIIAAGPIANFLFAIFAYWLVF
EEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHH
IIGVPGVRPVVGEISANSIAAQAQIAPGTELKAVDGIETPDWDSVRLQLVDKIGDESTTL
HHCCCCCCCHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCEE
SVAPFGSNQRRDVKLDLRQWSFEPDKEDPVSSLGIRPRGPQIEPVLVVIQANSAGMKAGL
EEEECCCCCCCEEEEEHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEEEEECCCCCCHHHH
QAGDRIVKVDGQPLTQWGTFVTLVRDNPGKPLALEIERQGNPLSLTLIPESKPGKGKAIG
CCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCEEE
FAGIEPKLIPLPEEYKIVRQYGPFNAILEATDKTWQLMKLTVSMLGKLITGDVNLNNLSG
ECCCCCCEECCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PISIAKGAGMTAELGIVYYLPFLALISVNLGIINLFPLPVLDGGHLLFLAIEKIKGGPVS
CEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHCCCCHH
ERVQDFSYRIGSILLVLLMGLALFNDFSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]