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The map label for this gene is btuF [H]

Identifier: 218547614

GI number: 218547614

Start: 199387

End: 200214

Strand: Reverse

Name: btuF [H]

Synonym: EFER_0181

Alternate gene names: 218547614

Gene position: 200214-199387 (Counterclockwise)

Preceding gene: 218547615

Following gene: 218547613

Centisome position: 4.36

GC content: 48.79

Gene sequence:

>828_bases
TTGAAACGCTGGTACAGAAACTGGCACATGGCTAATCTATCATTCAGGGCGCTCGCCGCCCTGTTCTTCTTGCTGCCACT
GTGGCTTTGTGCCGCACCGCGTGTTATTTCCCTCTCCCCTGCCAATACTGAACTGGTTTTTGCCGCCGGGATCACGCCTG
TTGGCGTCAGCAGCTATTCTGATTTTCCACCAGAAGCCAAAAACATTGAGCAAGTATCTACGTGGCAAGGAATCAATCTT
GAACGCATTGTCGCTCTAAAACCAGATCTCGTGGTCGCCTGGCGTGGCGGTAATGCCGAACGGCAAGTTAATCAGCTCTC
GTCGTTAGGTATAAAAGTGATGTGGGTGGATGCCTCAAACATTGAACAGATTTCTGATGCGTTGCTCAAACTTGCACCAT
GGAGTCCACACCCGGAGCAAGCTACGCAAGCCGCAGAACACCTGCTTGCCGAGTACGAAAGCCTCAAATCCCAGTTTGCC
GGTAAAGCGAAAAAGCGTATTTTTCTGCAGTTCGGCATCAATCCTCCATTTACTAGTGGAAAAGACTCAATTCAAAGCCA
AATTATTACCCTTTGTGGCGGAGAAAATATCTTTTCCGATAGCCGGGTTCCCTGGCCACAAGTCAGCCGTGAACAGGTAC
TGGCACGTGCTCCTCAGGCAATCGTGATAGCAGGAAACTCGAGCGATATTCCTAAAATCAAACAATACTGGGGCAATCAA
CTACAAATCCCGGTTATTCCGCTAAAAAGTGACTGGTTTGAAAGAGCAAGCCCGCGTATTATCCTCGCAGCAAAACAACT
CTGTACTGCCCTTTCACAGGTTAATTAG

Upstream 100 bases:

>100_bases
TTTGTGGTAGTACGTGCAATCTCCGACGTTGCTGATCAACAATCACACATTAGTTTTGATGAGTTCCTTGCTGTAGCTGC
GAAACAGTCAAGTCTGATGG

Downstream 100 bases:

>100_bases
AGTCTGCCTCGCAGGCTCTCAGTGGGGATTCGACTATGCTTGTCTATTGGTTGGATATTGTAGGCACAATAGCATTTGCC
ATCTCCGGCGTTATATTGGC

Product: vitamin B12-transporter protein BtuF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKRWYRNWHMANLSFRALAALFFLLPLWLCAAPRVISLSPANTELVFAAGITPVGVSSYSDFPPEAKNIEQVSTWQGINL
ERIVALKPDLVVAWRGGNAERQVNQLSSLGIKVMWVDASNIEQISDALLKLAPWSPHPEQATQAAEHLLAEYESLKSQFA
GKAKKRIFLQFGINPPFTSGKDSIQSQIITLCGGENIFSDSRVPWPQVSREQVLARAPQAIVIAGNSSDIPKIKQYWGNQ
LQIPVIPLKSDWFERASPRIILAAKQLCTALSQVN

Sequences:

>Translated_275_residues
MKRWYRNWHMANLSFRALAALFFLLPLWLCAAPRVISLSPANTELVFAAGITPVGVSSYSDFPPEAKNIEQVSTWQGINL
ERIVALKPDLVVAWRGGNAERQVNQLSSLGIKVMWVDASNIEQISDALLKLAPWSPHPEQATQAAEHLLAEYESLKSQFA
GKAKKRIFLQFGINPPFTSGKDSIQSQIITLCGGENIFSDSRVPWPQVSREQVLARAPQAIVIAGNSSDIPKIKQYWGNQ
LQIPVIPLKSDWFERASPRIILAAKQLCTALSQVN
>Mature_275_residues
MKRWYRNWHMANLSFRALAALFFLLPLWLCAAPRVISLSPANTELVFAAGITPVGVSSYSDFPPEAKNIEQVSTWQGINL
ERIVALKPDLVVAWRGGNAERQVNQLSSLGIKVMWVDASNIEQISDALLKLAPWSPHPEQATQAAEHLLAEYESLKSQFA
GKAKKRIFLQFGINPPFTSGKDSIQSQIITLCGGENIFSDSRVPWPQVSREQVLARAPQAIVIAGNSSDIPKIKQYWGNQ
LQIPVIPLKSDWFERASPRIILAAKQLCTALSQVN

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=266, Percent_Identity=78.5714285714286, Blast_Score=434, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 30556; Mature: 30556

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRWYRNWHMANLSFRALAALFFLLPLWLCAAPRVISLSPANTELVFAAGITPVGVSSYS
CCCHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCCCCC
DFPPEAKNIEQVSTWQGINLERIVALKPDLVVAWRGGNAERQVNQLSSLGIKVMWVDASN
CCCCCCCCHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEECCCC
IEQISDALLKLAPWSPHPEQATQAAEHLLAEYESLKSQFAGKAKKRIFLQFGINPPFTSG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCC
KDSIQSQIITLCGGENIFSDSRVPWPQVSREQVLARAPQAIVIAGNSSDIPKIKQYWGNQ
HHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHCCCE
LQIPVIPLKSDWFERASPRIILAAKQLCTALSQVN
EEEEEEECCCHHHHHCCCEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MKRWYRNWHMANLSFRALAALFFLLPLWLCAAPRVISLSPANTELVFAAGITPVGVSSYS
CCCHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCCCCC
DFPPEAKNIEQVSTWQGINLERIVALKPDLVVAWRGGNAERQVNQLSSLGIKVMWVDASN
CCCCCCCCHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEECCCC
IEQISDALLKLAPWSPHPEQATQAAEHLLAEYESLKSQFAGKAKKRIFLQFGINPPFTSG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCC
KDSIQSQIITLCGGENIFSDSRVPWPQVSREQVLARAPQAIVIAGNSSDIPKIKQYWGNQ
HHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHCCCE
LQIPVIPLKSDWFERASPRIILAAKQLCTALSQVN
EEEEEEECCCHHHHHCCCEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]