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The map label for this gene is ecpD [H]

Identifier: 218547596

GI number: 218547596

Start: 177463

End: 178203

Strand: Reverse

Name: ecpD [H]

Synonym: EFER_0163

Alternate gene names: 218547596

Gene position: 178203-177463 (Counterclockwise)

Preceding gene: 218547597

Following gene: 218547595

Centisome position: 3.88

GC content: 45.88

Gene sequence:

>741_bases
ATGTTTTTCAACACTAAACATACAGCAGCATTATGCTTCGCAACCTGTGTAGCTTTTAGCTCATCAGCTATAGCAGATAT
CGTAATTTCCGGTACACGCGTAATCTATAAAAGCGACCAAAAAAGTGTCAGCGTTCGTCTGGAAAATAAAGGCAATAACC
CATTACTTGTTCAAAGTTGGCTGGATACCGGCGATGACAATGCTGAGCCTGGCAGTATTACCGTACCCTTTACTGCGACT
CCGCCCGTATCACGCATTGATGCAAAACGCGGCCAAACTATCAAACTGATGTACACCGCCAGCAGCGTCCTGCCAAAAGA
CAGAGAAAGTGTGTTCTGGTTTAACGTACTGGAAGTTCCTCCAAAACCAGATGCCGCAACAACTACAAATCAAAGCCTGC
TGCAGTTGGCATTTCGTACCCGTATCAAATTGTTTTATCGTCCTGAAGGGTTAAAAGGCAATCCGTCTGACGCGCCGTTA
GCACTTAAATGGACATGGGCAACTGCGGGAGGCCAAACAGCTTTGCGAGTAGCAAACCCTACGCCTTATTACGTCTCTTT
TAGCAGTGGTGATCTAGAAACCAGTGGCAAGCGCTATCCACTCGACGTGAAGATGATTGCGCCATTCAGTAACGATGTCA
TCAAGGTGAATGGTATGAGTGGCAAATCAAGCTCTGCAAAAGTTCATTTCTACGCCATTAATGATTTTGGTGGCGCAATT
GAAGGCAATGCAACCCTGTAG

Upstream 100 bases:

>100_bases
GTAAGCATTTCCATCTATATTCATGAATTGAATACATACAGGGAATAATATTGATATTTCATACTACTCCCTGTTTTTTT
AACTCTGCCAGGAATGGTTT

Downstream 100 bases:

>100_bases
TCAGGCAGGGGATATAACGCAGCAGGAAGCGAAACGTGCAAAAAATAACAACAACTCATCCTTACAACCTCACGCAGATC
GCTACGCTTTGTGCGCTGCT

Product: chaperone protein EcpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MFFNTKHTAALCFATCVAFSSSAIADIVISGTRVIYKSDQKSVSVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASSVLPKDRESVFWFNVLEVPPKPDAATTTNQSLLQLAFRTRIKLFYRPEGLKGNPSDAPL
ALKWTWATAGGQTALRVANPTPYYVSFSSGDLETSGKRYPLDVKMIAPFSNDVIKVNGMSGKSSSAKVHFYAINDFGGAI
EGNATL

Sequences:

>Translated_246_residues
MFFNTKHTAALCFATCVAFSSSAIADIVISGTRVIYKSDQKSVSVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASSVLPKDRESVFWFNVLEVPPKPDAATTTNQSLLQLAFRTRIKLFYRPEGLKGNPSDAPL
ALKWTWATAGGQTALRVANPTPYYVSFSSGDLETSGKRYPLDVKMIAPFSNDVIKVNGMSGKSSSAKVHFYAINDFGGAI
EGNATL
>Mature_246_residues
MFFNTKHTAALCFATCVAFSSSAIADIVISGTRVIYKSDQKSVSVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASSVLPKDRESVFWFNVLEVPPKPDAATTTNQSLLQLAFRTRIKLFYRPEGLKGNPSDAPL
ALKWTWATAGGQTALRVANPTPYYVSFSSGDLETSGKRYPLDVKMIAPFSNDVIKVNGMSGKSSSAKVHFYAINDFGGAI
EGNATL

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=246, Percent_Identity=86.9918699186992, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1788428, Length=241, Percent_Identity=34.4398340248963, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1789532, Length=238, Percent_Identity=34.453781512605, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788677, Length=214, Percent_Identity=37.8504672897196, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1786936, Length=209, Percent_Identity=30.1435406698565, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1790771, Length=231, Percent_Identity=29.8701298701299, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786743, Length=239, Percent_Identity=31.3807531380753, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI87081806, Length=233, Percent_Identity=32.1888412017167, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1787171, Length=227, Percent_Identity=29.5154185022026, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082203, Length=243, Percent_Identity=28.8065843621399, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26666; Mature: 26666

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFNTKHTAALCFATCVAFSSSAIADIVISGTRVIYKSDQKSVSVRLENKGNNPLLVQSW
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEECEEEEEECCCCEEEEEEECCCCCCEEEEEE
LDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASSVLPKDRESVFWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEEEEECC
PKPDAATTTNQSLLQLAFRTRIKLFYRPEGLKGNPSDAPLALKWTWATAGGQTALRVANP
CCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCC
TPYYVSFSSGDLETSGKRYPLDVKMIAPFSNDVIKVNGMSGKSSSAKVHFYAINDFGGAI
CCEEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEEECCCCEE
EGNATL
CCCCCC
>Mature Secondary Structure
MFFNTKHTAALCFATCVAFSSSAIADIVISGTRVIYKSDQKSVSVRLENKGNNPLLVQSW
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEECEEEEEECCCCEEEEEEECCCCCCEEEEEE
LDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASSVLPKDRESVFWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEEEEECC
PKPDAATTTNQSLLQLAFRTRIKLFYRPEGLKGNPSDAPLALKWTWATAGGQTALRVANP
CCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCC
TPYYVSFSSGDLETSGKRYPLDVKMIAPFSNDVIKVNGMSGKSSSAKVHFYAINDFGGAI
CCEEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEEECCCCEE
EGNATL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]