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The map label for this gene is fixJ [H]

Identifier: 218533234

GI number: 218533234

Start: 5749559

End: 5751490

Strand: Reverse

Name: fixJ [H]

Synonym: Mchl_5362

Alternate gene names: 218533234

Gene position: 5751490-5749559 (Counterclockwise)

Preceding gene: 218533237

Following gene: 218533232

Centisome position: 99.54

GC content: 67.24

Gene sequence:

>1932_bases
ATGAATTCCTCCGGGACGGGCCTGGACGCAAAATCCGGCCGATCGCCTGATGTCTCTGCGGCGAAGCGGATCGCGGCGAT
CCCCACCATGCTCGAGATCGTCTGTCGCTCCACGGGCCTGGGCTTTGCAGCCGTGGCGCAGGTGACGGACGAGACGTGGC
TGGCTTGCCGGGTCAGGGATGACGCCGGTTTCGGCCTTCAGGCGGACGCCGTGCTTCGGGCGGACGCGATGCCCTGCCGA
GAAGTGAGGCGGCATCGACGGCCGGTCGTCATCGACGATGCCGGACCGGATACGGCAATGCGTGGACTTTCGAGTTCCAT
TTCTCTCGGCTTTCGCAGCTACATGTCCTTTCCAATCACGATGGCAGATGGGGAGATCTTCGGAACACTTTGCGCCATCG
GAAAGGAGGCCGCCCATCTGGATCGCCCGGAGATCACGGGCCTGTTCCAGCAATTCTCCGAACTGATCGGACTTCTCCTC
GACGCGCAGCGGCAGCCCCCCGTCGCCAGCCCTCGGCCTGAGGAGGCGGGGTTCGCATCGTGGGAGCGGAACGAGCCGTT
GAGGGCGCTGATCGATCACCTGCCGGTCGGCGCTGGGCTGTTCGGGTCCGACGGTCAGGCCCTCGTCGCAAATGCCATCC
TTCAGCCTCTTCTGTCACAGGGCCGCGTGCCCGGTGCCGATCCCGATGCGGCCCCGCATTGGACCGGCTATGCGGGGGAC
GGCACCGTCCTCGATCCGCGCGATCATCCGTTTGCACGGGCTTTGCGTGGCGATCGCGTCCTAGGCACGCGCTTCCATCA
CCGCGGGGATGACGGAACCGGGCGGTGGACACGGATCAGCGGGGTGCCTCTCTCGGGGACGACCGACGACGCACGCACCC
GGAACGGAAAGGAGCCGGCCGTACTCCTCTTCGTCGAGGAAGAGCGCGAGGCGGAAGAAACCCGGCGCGCGAGCGACGAG
CGCTTCCGGCGATTTGCCAAACATTCGACCAACGTCTTGTGGCTCGCCGACTTGGAGAGCCGCCGGCTGACTTACCTCAA
TCATGCCCTCCGGCGCGTCTGGGGCGTCACGCCCGAGCGGCTGCCCTCCCTCGAAAGCTGGCTTGAAACGATCCATTCGG
ACGACCGCGACAGCGTTGAGCGCACGCTCGAACGGGCCTGGGATGGTGCGGCCGTCGTCCAAGAGTACCGCATCCTGCGC
CCGTCGGACGGAGCTGTGAGGCGGATCCGCGAGACAGTCTTCCCGATCGCCAGGGAGGATGGCCGCGTTCGCCAACTGGG
TGGCATCGCCGAAGATGTCGCGGGTCACACCGGCGCACGGATCTACCTCGTGGACGAGGATGCGCCCGCGCGACGGGCGC
TGCTCGGGCTGCTGCAACGCGCTGGCTACGAGGTGCAGGTCTTCGCGAAGGCCGCAGCGCTGGCGGAGGTCGCAAGCGCC
CTGCAGCCGGGTTGCGTCATTCTCGACATCGAGACCGCCGGACCGGAGAGCCTCACGGTAGCGAAAGCGTTCAGGGCCAG
CCGGCTCGCGCTGCCGGTGGTCGTGTTCGGGCGCAGCCATGGCGATGTCGGCTTCGGTGTGCGCGCCATGAAGGCCGGCG
CCGTCGATTACCTTGAAAAGCCGTGCGAGCCGACAGTGCTGCTCACCGCCGTTTCGGCCGCCTTGGCCGAACTGCGCACC
GACACCGAGCGCAGCCACGCACATGACGACGCCAAGCTTCGCATCGCGGCCCTATCACCCCGAGAACGGGAAGTGCTGGA
GGGGTTGCTGGCAGGCGGCACGAACAAGTCGATCGGCCGGGCACTCGGCCTCAGTCCGCGCACCGTGGAGATCCACCGAG
CCCGCGTGATGGAAGCGCTCGGGGCGCGCACCCTGCCGGAGGCGGTGCTGACGGCAGCTAGGGCCGGGGTGCAACCCACC
GAAAGTCAATGA

Upstream 100 bases:

>100_bases
CTGTGTGGATAAGGACCGAGATATCCGTCATCCGCCTCGATTTACGCATGCCTCATTCGAAGTCGCATGCCGTGATGGGA
TCGGGGCGGGAGTTCACCCA

Downstream 100 bases:

>100_bases
GGGGCAAGGGGGGAGGGACGTGGGAGCCGCCGCTTCGGCGCAGAATGCGTCAGGCGGTGAGTTTGTCGATGGCGGAGGGC
CGCAGCGGGACCGCGGCCGG

Product: LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MNSSGTGLDAKSGRSPDVSAAKRIAAIPTMLEIVCRSTGLGFAAVAQVTDETWLACRVRDDAGFGLQADAVLRADAMPCR
EVRRHRRPVVIDDAGPDTAMRGLSSSISLGFRSYMSFPITMADGEIFGTLCAIGKEAAHLDRPEITGLFQQFSELIGLLL
DAQRQPPVASPRPEEAGFASWERNEPLRALIDHLPVGAGLFGSDGQALVANAILQPLLSQGRVPGADPDAAPHWTGYAGD
GTVLDPRDHPFARALRGDRVLGTRFHHRGDDGTGRWTRISGVPLSGTTDDARTRNGKEPAVLLFVEEEREAEETRRASDE
RFRRFAKHSTNVLWLADLESRRLTYLNHALRRVWGVTPERLPSLESWLETIHSDDRDSVERTLERAWDGAAVVQEYRILR
PSDGAVRRIRETVFPIAREDGRVRQLGGIAEDVAGHTGARIYLVDEDAPARRALLGLLQRAGYEVQVFAKAAALAEVASA
LQPGCVILDIETAGPESLTVAKAFRASRLALPVVVFGRSHGDVGFGVRAMKAGAVDYLEKPCEPTVLLTAVSAALAELRT
DTERSHAHDDAKLRIAALSPREREVLEGLLAGGTNKSIGRALGLSPRTVEIHRARVMEALGARTLPEAVLTAARAGVQPT
ESQ

Sequences:

>Translated_643_residues
MNSSGTGLDAKSGRSPDVSAAKRIAAIPTMLEIVCRSTGLGFAAVAQVTDETWLACRVRDDAGFGLQADAVLRADAMPCR
EVRRHRRPVVIDDAGPDTAMRGLSSSISLGFRSYMSFPITMADGEIFGTLCAIGKEAAHLDRPEITGLFQQFSELIGLLL
DAQRQPPVASPRPEEAGFASWERNEPLRALIDHLPVGAGLFGSDGQALVANAILQPLLSQGRVPGADPDAAPHWTGYAGD
GTVLDPRDHPFARALRGDRVLGTRFHHRGDDGTGRWTRISGVPLSGTTDDARTRNGKEPAVLLFVEEEREAEETRRASDE
RFRRFAKHSTNVLWLADLESRRLTYLNHALRRVWGVTPERLPSLESWLETIHSDDRDSVERTLERAWDGAAVVQEYRILR
PSDGAVRRIRETVFPIAREDGRVRQLGGIAEDVAGHTGARIYLVDEDAPARRALLGLLQRAGYEVQVFAKAAALAEVASA
LQPGCVILDIETAGPESLTVAKAFRASRLALPVVVFGRSHGDVGFGVRAMKAGAVDYLEKPCEPTVLLTAVSAALAELRT
DTERSHAHDDAKLRIAALSPREREVLEGLLAGGTNKSIGRALGLSPRTVEIHRARVMEALGARTLPEAVLTAARAGVQPT
ESQ
>Mature_643_residues
MNSSGTGLDAKSGRSPDVSAAKRIAAIPTMLEIVCRSTGLGFAAVAQVTDETWLACRVRDDAGFGLQADAVLRADAMPCR
EVRRHRRPVVIDDAGPDTAMRGLSSSISLGFRSYMSFPITMADGEIFGTLCAIGKEAAHLDRPEITGLFQQFSELIGLLL
DAQRQPPVASPRPEEAGFASWERNEPLRALIDHLPVGAGLFGSDGQALVANAILQPLLSQGRVPGADPDAAPHWTGYAGD
GTVLDPRDHPFARALRGDRVLGTRFHHRGDDGTGRWTRISGVPLSGTTDDARTRNGKEPAVLLFVEEEREAEETRRASDE
RFRRFAKHSTNVLWLADLESRRLTYLNHALRRVWGVTPERLPSLESWLETIHSDDRDSVERTLERAWDGAAVVQEYRILR
PSDGAVRRIRETVFPIAREDGRVRQLGGIAEDVAGHTGARIYLVDEDAPARRALLGLLQRAGYEVQVFAKAAALAEVASA
LQPGCVILDIETAGPESLTVAKAFRASRLALPVVVFGRSHGDVGFGVRAMKAGAVDYLEKPCEPTVLLTAVSAALAELRT
DTERSHAHDDAKLRIAALSPREREVLEGLLAGGTNKSIGRALGLSPRTVEIHRARVMEALGARTLPEAVLTAARAGVQPT
ESQ

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 69591; Mature: 69591

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSGTGLDAKSGRSPDVSAAKRIAAIPTMLEIVCRSTGLGFAAVAQVTDETWLACRVRD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEC
DAGFGLQADAVLRADAMPCREVRRHRRPVVIDDAGPDTAMRGLSSSISLGFRSYMSFPIT
CCCCCCCCCHHHHCCCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEE
MADGEIFGTLCAIGKEAAHLDRPEITGLFQQFSELIGLLLDAQRQPPVASPRPEEAGFAS
EECCHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
WERNEPLRALIDHLPVGAGLFGSDGQALVANAILQPLLSQGRVPGADPDAAPHWTGYAGD
CCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GTVLDPRDHPFARALRGDRVLGTRFHHRGDDGTGRWTRISGVPLSGTTDDARTRNGKEPA
CCEECCCCCHHHHHHCCCCEECCHHCCCCCCCCCCEEEECCCCCCCCCCHHHCCCCCCCE
VLLFVEEEREAEETRRASDERFRRFAKHSTNVLWLADLESRRLTYLNHALRRVWGVTPER
EEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHH
LPSLESWLETIHSDDRDSVERTLERAWDGAAVVQEYRILRPSDGAVRRIRETVFPIARED
CCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
GRVRQLGGIAEDVAGHTGARIYLVDEDAPARRALLGLLQRAGYEVQVFAKAAALAEVASA
CCHHHHCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHH
LQPGCVILDIETAGPESLTVAKAFRASRLALPVVVFGRSHGDVGFGVRAMKAGAVDYLEK
CCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCHHHHHC
PCEPTVLLTAVSAALAELRTDTERSHAHDDAKLRIAALSPREREVLEGLLAGGTNKSIGR
CCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHH
ALGLSPRTVEIHRARVMEALGARTLPEAVLTAARAGVQPTESQ
HHCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNSSGTGLDAKSGRSPDVSAAKRIAAIPTMLEIVCRSTGLGFAAVAQVTDETWLACRVRD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEC
DAGFGLQADAVLRADAMPCREVRRHRRPVVIDDAGPDTAMRGLSSSISLGFRSYMSFPIT
CCCCCCCCCHHHHCCCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEE
MADGEIFGTLCAIGKEAAHLDRPEITGLFQQFSELIGLLLDAQRQPPVASPRPEEAGFAS
EECCHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
WERNEPLRALIDHLPVGAGLFGSDGQALVANAILQPLLSQGRVPGADPDAAPHWTGYAGD
CCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GTVLDPRDHPFARALRGDRVLGTRFHHRGDDGTGRWTRISGVPLSGTTDDARTRNGKEPA
CCEECCCCCHHHHHHCCCCEECCHHCCCCCCCCCCEEEECCCCCCCCCCHHHCCCCCCCE
VLLFVEEEREAEETRRASDERFRRFAKHSTNVLWLADLESRRLTYLNHALRRVWGVTPER
EEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHH
LPSLESWLETIHSDDRDSVERTLERAWDGAAVVQEYRILRPSDGAVRRIRETVFPIARED
CCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
GRVRQLGGIAEDVAGHTGARIYLVDEDAPARRALLGLLQRAGYEVQVFAKAAALAEVASA
CCHHHHCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHH
LQPGCVILDIETAGPESLTVAKAFRASRLALPVVVFGRSHGDVGFGVRAMKAGAVDYLEK
CCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCHHHHHC
PCEPTVLLTAVSAALAELRTDTERSHAHDDAKLRIAALSPREREVLEGLLAGGTNKSIGR
CCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHH
ALGLSPRTVEIHRARVMEALGARTLPEAVLTAARAGVQPTESQ
HHCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]