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The map label for this gene is mutB [H]

Identifier: 218533135

GI number: 218533135

Start: 5633128

End: 5635293

Strand: Reverse

Name: mutB [H]

Synonym: Mchl_5261

Alternate gene names: 218533135

Gene position: 5635293-5633128 (Counterclockwise)

Preceding gene: 218533136

Following gene: 218533134

Centisome position: 97.53

GC content: 67.82

Gene sequence:

>2166_bases
ATGAGTTCCCGCATCCCCGATTTCGCCTCCGTCGCCTACGCGGCCGACGGCTTCAAGGCGCCGCCCCCGCCGGCCGGGGA
GCCGTGGATGACGCCGGAGGGCATTCCGGTGAAGGGCTTCTACGGCCCCGAGGACCGCGAGGGCTGCGAGGGGATCGACT
CGTTCCCCGGCCTGCCGCCCTACCTGCGCGGTCCCTATCCGGCGATGTACGTGACCCAGCCCTGGACGATCCGGCAATAT
GCCGGCTTCTCCACGGCCGAGGATTCGAACGCCTTCTACCGGCGCAACCTCGCGGCGGGCCAGAAGGGCCTCTCGGTCGC
CTTCGATCTCGCCACCCACCGAGGCTACGATTCGGACCACCCGCGCGTCTCGGGTGATGTCGGCATGGCGGGTGTCGCGA
TCGACTCGATCTACGACATGCGCACGCTGTTCTCCGGCATTCCGCTGGACGAGATGACGGTGTCGATGACGATGAACGGC
GCGGTGCTGCCGGTGCTCGCGCTCTACATCGTCGCGGCCGAGGAGCAGGGCGTGCCGCCCGAGAAGCTCGCCGGCACGAT
CCAGAACGACATCCTCAAGGAGTTCATGGTCCGCAACACCTACATCTATCCCCCCAAGGGATCGATGCGGATCATCTCGG
ACATCTTCGGCTACACCTCGAAGAACATGCCGAAGTTCAACTCGATCTCGATCTCCGGCTACCACATGCAGGAGGCCGGG
GCGACGCAGGATCTGGAGCTCGCCTACACGCTCGCCGACGGCGTCGAGTACATCAAGGCCGGCCTCGCGGCGGGCCTCAC
CATTGATCAGTTCGCCCCGCGCCTGTCCTTCTTCTGGGCGATCGGGATGAACTTCTTCATGGAGATCGCCAAGATGCGGG
CCGCGCGCCTGATCTGGGCCAAGCTCGTCAAGGAGTTCGAGCCGAAATCTGACAAGTCGCTGCCGCTGCGGACCCACTCG
CAGACGAGTGGCTGGTCGCTGACGGCGCAGGACGTGTTCAACAACGTCACCCGCACCTGCATCGAGGCGATGGCGGCGAC
GCAGGGCGGCACCCAGTCGCTCCACACCAACGCGCTCGACGAGGCTTTGGCGCTGCCGACCGACTTCTCGGCGCGCATCG
CCCGTAACACCCAGCTCTTCCTGCAGCAGGAGAGCGGCACCACGCGGATCATCGATCCGTGGGGCGGCTCCTACTATGTC
GAGCGGCTGACCCGGGACATCGCCGCGCGCGCCTGGGAGCATATCCGCGAGGTCGAGGCGCTCGGCGGCATGGCGAAAGC
CATCGAGGCCGGCATCCCGAAGCTGCGCATCGAGGAGGCCGCCGCCCGCGCCCAGGCGCGGATCGATTCCGGGCGCCAGA
CCATCGTCGGCATCAACAAGTACAAGCCTGACGACGAGATGAAGATCGACCTGCTGCGCGTCGATAACGCCGACGTGCGC
GCCAAGCAGATCGACAAGCTCAAGCGCCTGCGCGCCGAGCGCAACCAGGCCGACGTCGATGCGGCTCTGGCCGCGCTGAC
GAAGGCCGCGGATGGCGAGGGCAACCTGCTCGAACTGGCGGTGAACGCGGCGCGGGCCAAGGCCACGGTCGGCGAGATCT
CGGAGGCGCTGGAGAAGGCTTGGGGCCGTCACCGCGCCGAGATCCGCTCGATCTCGGGCGTCTACAAGCGGGAGGTGGGC
GGTATGTCGCCGGTGGTGGAGAAGGTCCGCGGCCTCGTCGAGGCCTTCGAGGAGAATGACGGGCGCCGCCCGCGCATCCT
GGTCGCCAAGATGGGCCAGGACGGGCACGACCGCGGCCAGAAGGTGATCGCCTCGGCGTTTGCCGATCTTGGCTTCGACG
TCGATATCGGGCCGCTCTTCGCCACGCCGGACGAGGCCGCGCGACAGGCGGTGGAGAACGACGTGCACATCGTCGGCGTC
TCCTCGCTGGCGGCGGGCCACCTGACGCTGGTGCCGGAACTCAAGGCCGCGCTGAAGCAGGAGGGCCGCGACGACGTGAT
GATCGTGGTCGGCGGCGTGATCCCGCCGGGCGACTACGATGCGCTCTACGCCGCGGGCGCCTCGGCGATCTTCCCGCCGG
GCACCGTGATCGCGGAAGCGGCCGTGAAGCTTCTGGGCGAACTCAACACGCGCCTGGGCTACGGCGAGCGGCAAGCGGCC
GAGTAG

Upstream 100 bases:

>100_bases
GCCTTATGGACAGTAGCCGCGTTTGGTGGCTTGCCTATAATGCTCAAAAAAAATCATCGTCTCCGCCGATGCCGAGACTG
ACGCCAGGAACAGACGCACG

Downstream 100 bases:

>100_bases
TCCGCTTTTCCAGTCTCGGCGGGGGTGATACCCCCGCCTTTCATTTGAGTTTACCGGGATTCCAAGCGGCACCATGCCTC
GCGTGGTGCCGCCTCATGGG

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MSSRIPDFASVAYAADGFKAPPPPAGEPWMTPEGIPVKGFYGPEDREGCEGIDSFPGLPPYLRGPYPAMYVTQPWTIRQY
AGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVSGDVGMAGVAIDSIYDMRTLFSGIPLDEMTVSMTMNG
AVLPVLALYIVAAEEQGVPPEKLAGTIQNDILKEFMVRNTYIYPPKGSMRIISDIFGYTSKNMPKFNSISISGYHMQEAG
ATQDLELAYTLADGVEYIKAGLAAGLTIDQFAPRLSFFWAIGMNFFMEIAKMRAARLIWAKLVKEFEPKSDKSLPLRTHS
QTSGWSLTAQDVFNNVTRTCIEAMAATQGGTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYV
ERLTRDIAARAWEHIREVEALGGMAKAIEAGIPKLRIEEAAARAQARIDSGRQTIVGINKYKPDDEMKIDLLRVDNADVR
AKQIDKLKRLRAERNQADVDAALAALTKAADGEGNLLELAVNAARAKATVGEISEALEKAWGRHRAEIRSISGVYKREVG
GMSPVVEKVRGLVEAFEENDGRRPRILVAKMGQDGHDRGQKVIASAFADLGFDVDIGPLFATPDEAARQAVENDVHIVGV
SSLAAGHLTLVPELKAALKQEGRDDVMIVVGGVIPPGDYDALYAAGASAIFPPGTVIAEAAVKLLGELNTRLGYGERQAA
E

Sequences:

>Translated_721_residues
MSSRIPDFASVAYAADGFKAPPPPAGEPWMTPEGIPVKGFYGPEDREGCEGIDSFPGLPPYLRGPYPAMYVTQPWTIRQY
AGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVSGDVGMAGVAIDSIYDMRTLFSGIPLDEMTVSMTMNG
AVLPVLALYIVAAEEQGVPPEKLAGTIQNDILKEFMVRNTYIYPPKGSMRIISDIFGYTSKNMPKFNSISISGYHMQEAG
ATQDLELAYTLADGVEYIKAGLAAGLTIDQFAPRLSFFWAIGMNFFMEIAKMRAARLIWAKLVKEFEPKSDKSLPLRTHS
QTSGWSLTAQDVFNNVTRTCIEAMAATQGGTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYV
ERLTRDIAARAWEHIREVEALGGMAKAIEAGIPKLRIEEAAARAQARIDSGRQTIVGINKYKPDDEMKIDLLRVDNADVR
AKQIDKLKRLRAERNQADVDAALAALTKAADGEGNLLELAVNAARAKATVGEISEALEKAWGRHRAEIRSISGVYKREVG
GMSPVVEKVRGLVEAFEENDGRRPRILVAKMGQDGHDRGQKVIASAFADLGFDVDIGPLFATPDEAARQAVENDVHIVGV
SSLAAGHLTLVPELKAALKQEGRDDVMIVVGGVIPPGDYDALYAAGASAIFPPGTVIAEAAVKLLGELNTRLGYGERQAA
E
>Mature_720_residues
SSRIPDFASVAYAADGFKAPPPPAGEPWMTPEGIPVKGFYGPEDREGCEGIDSFPGLPPYLRGPYPAMYVTQPWTIRQYA
GFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVSGDVGMAGVAIDSIYDMRTLFSGIPLDEMTVSMTMNGA
VLPVLALYIVAAEEQGVPPEKLAGTIQNDILKEFMVRNTYIYPPKGSMRIISDIFGYTSKNMPKFNSISISGYHMQEAGA
TQDLELAYTLADGVEYIKAGLAAGLTIDQFAPRLSFFWAIGMNFFMEIAKMRAARLIWAKLVKEFEPKSDKSLPLRTHSQ
TSGWSLTAQDVFNNVTRTCIEAMAATQGGTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVE
RLTRDIAARAWEHIREVEALGGMAKAIEAGIPKLRIEEAAARAQARIDSGRQTIVGINKYKPDDEMKIDLLRVDNADVRA
KQIDKLKRLRAERNQADVDAALAALTKAADGEGNLLELAVNAARAKATVGEISEALEKAWGRHRAEIRSISGVYKREVGG
MSPVVEKVRGLVEAFEENDGRRPRILVAKMGQDGHDRGQKVIASAFADLGFDVDIGPLFATPDEAARQAVENDVHIVGVS
SLAAGHLTLVPELKAALKQEGRDDVMIVVGGVIPPGDYDALYAAGASAIFPPGTVIAEAAVKLLGELNTRLGYGERQAAE

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=686, Percent_Identity=63.9941690962099, Blast_Score=907, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=679, Percent_Identity=62.8865979381443, Blast_Score=843, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=690, Percent_Identity=64.4927536231884, Blast_Score=917, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 78219; Mature: 78088

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRIPDFASVAYAADGFKAPPPPAGEPWMTPEGIPVKGFYGPEDREGCEGIDSFPGLPP
CCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
YLRGPYPAMYVTQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDH
CCCCCCCEEEEECCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCC
PRVSGDVGMAGVAIDSIYDMRTLFSGIPLDEMTVSMTMNGAVLPVLALYIVAAEEQGVPP
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHHHCCCCCCCH
EKLAGTIQNDILKEFMVRNTYIYPPKGSMRIISDIFGYTSKNMPKFNSISISGYHMQEAG
HHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECEEECCCC
ATQDLELAYTLADGVEYIKAGLAAGLTIDQFAPRLSFFWAIGMNFFMEIAKMRAARLIWA
CCCCHHEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVKEFEPKSDKSLPLRTHSQTSGWSLTAQDVFNNVTRTCIEAMAATQGGTQSLHTNALD
HHHHHHCCCCCCCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVERLTRDIAARAWEHIREVEA
HHHCCCCCCHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGGMAKAIEAGIPKLRIEEAAARAQARIDSGRQTIVGINKYKPDDEMKIDLLRVDNADVR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEECCCCHH
AKQIDKLKRLRAERNQADVDAALAALTKAADGEGNLLELAVNAARAKATVGEISEALEKA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
WGRHRAEIRSISGVYKREVGGMSPVVEKVRGLVEAFEENDGRRPRILVAKMGQDGHDRGQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
KVIASAFADLGFDVDIGPLFATPDEAARQAVENDVHIVGVSSLAAGHLTLVPELKAALKQ
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEHHHCCCCEEECHHHHHHHHH
EGRDDVMIVVGGVIPPGDYDALYAAGASAIFPPGTVIAEAAVKLLGELNTRLGYGERQAA
CCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCC
E
C
>Mature Secondary Structure 
SSRIPDFASVAYAADGFKAPPPPAGEPWMTPEGIPVKGFYGPEDREGCEGIDSFPGLPP
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
YLRGPYPAMYVTQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDH
CCCCCCCEEEEECCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCC
PRVSGDVGMAGVAIDSIYDMRTLFSGIPLDEMTVSMTMNGAVLPVLALYIVAAEEQGVPP
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHHHCCCCCCCH
EKLAGTIQNDILKEFMVRNTYIYPPKGSMRIISDIFGYTSKNMPKFNSISISGYHMQEAG
HHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECEEECCCC
ATQDLELAYTLADGVEYIKAGLAAGLTIDQFAPRLSFFWAIGMNFFMEIAKMRAARLIWA
CCCCHHEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVKEFEPKSDKSLPLRTHSQTSGWSLTAQDVFNNVTRTCIEAMAATQGGTQSLHTNALD
HHHHHHCCCCCCCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVERLTRDIAARAWEHIREVEA
HHHCCCCCCHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGGMAKAIEAGIPKLRIEEAAARAQARIDSGRQTIVGINKYKPDDEMKIDLLRVDNADVR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEECCCCHH
AKQIDKLKRLRAERNQADVDAALAALTKAADGEGNLLELAVNAARAKATVGEISEALEKA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
WGRHRAEIRSISGVYKREVGGMSPVVEKVRGLVEAFEENDGRRPRILVAKMGQDGHDRGQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
KVIASAFADLGFDVDIGPLFATPDEAARQAVENDVHIVGVSSLAAGHLTLVPELKAALKQ
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEHHHCCCCEEECHHHHHHHHH
EGRDDVMIVVGGVIPPGDYDALYAAGASAIFPPGTVIAEAAVKLLGELNTRLGYGERQAA
CCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]