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The map label for this gene is pit [H]

Identifier: 218533129

GI number: 218533129

Start: 5627227

End: 5628867

Strand: Reverse

Name: pit [H]

Synonym: Mchl_5255

Alternate gene names: 218533129

Gene position: 5628867-5627227 (Counterclockwise)

Preceding gene: 218533134

Following gene: 218533126

Centisome position: 97.42

GC content: 67.58

Gene sequence:

>1641_bases
ATGTCAGACGCGCTCAGCCTGCAGGACGGGGGTTCGGCACCGACCGAAGCCCGCTCGCCACGGGGCCCGAATCTCGATGG
CCGGATGCATCCGGCTGGAATCGGCCTCTTCTTGCTCGTTCTGGTGGGAGGGCTCGGCTACGCGCTCTTCAACCTGATCA
CGGACATGCAGGCCGCCGGCCAGCCGCCGCTGGCGATCGGTGCCTTCGCGCTGCTCGGCCTCGCGCTGCTCATCGCTCTC
GGCTTCGAGTTCGTGAACGGCTTCCACGACACCGCCAACGCCGTCGCGACAGTGATCTACACCCACAGCCTGCCTCCGGT
CGTCGCCGTGGTCTATTCCGGCGTCTTCAACTTTCTCGGCGTCCTCGTCTCGACCGGTGCCGTCGCCTACAGCGTCGTCA
CCTTGCTGCCCGTGGAACTGGTGCTGAAGTCCGGTTCGTCCGCCGGTTACGCGATGATCTTCGCGCTGCTCATCGCCGCG
ATCATCTGGAACCTGGGGACATGGGCGCTCGGCCTGCCGAACTCGTCCTCGCACGCGCTGATCGGATCGATTCTCGGCGT
GGGCCTTGCCAACCAGCTCCTCGCCGCCGAAGGCTCGGGCACCTCCGGTGTCCAGTGGAATCAGGCGTCGAGCGTGCTGC
GGGCCCTGCTGTTCAGCCCCCTCGTCGGCTTCGTTATGGCGGCCCTGCTGCTGCTGACGATGCGGTTCGTGATCCGCAAC
CGCACCCTCTACCAGGAGCCGGAGGGTGACACGCCGCCCCCGCTCTGGATCCGCGCCCTACTGATCCTCACCTGCGGCGG
CGTCTCCTTCGCCCATGGCGGCAATGACGGGCAGAAGGGCATGGGCCTGATCATGCTGATCCTGATCGGCGCCGCGCCCA
CCGCCTACGCGCTCAACCGGACCATGTCGGAATCGGCAACGCCGCATTTCGTGCAAGTCTCGGAGCGCGCCCGGACGGTG
TTCCAGAACCGGGCGGACCAAGCCCCGATGCCGAGCCCGGCCGAGGGGCGCGTGCAGATCACCGAGGCGCTAGGGAAGAA
GGACCTCGACCGGCCGGAGACCTATGCGGCCCTGGCCGCCCTGTCGGACGACATCGCCACGAAGGTGAAGGAGTACGGCG
CGATCCGCCACGTTCCAGCGGAGGCGACGCCGAACTTGCGCAACGACATGTACCTCGCGTCGGACGCGATCCACAGCCTG
AAGAAGCACGAGGGGCTCTTCGTCGGCGAGGAGGCCGAGATGCTGAAGGCCTATGAGACGAGCCTGAATGACGGCACCCG
CTACATCCCGACCTGGGTGAAAGTCTCGGTCGCCCTGGCGCTCGGCCTCGGTACGATGGTGGGCTGGAAGCGCATCGTCG
TCACGGTCGGCAGCCGCATCGGCAAGAAGCACCTGACCTACGCGCAAGGGGCGTCCGCCGAGATCACGGCCGCGGGCACG
ATCGGACTTGCGGAGGCTTACGGCCTGCCGGTCTCGACCACTCACATCCTGTCGAGCGGCGTCGCCGGCACCATGGCCGC
CAACGGCTCGGGCCTGCAATGGGCCACCGTGCGCAACCTAGCCGCCGCATGGCTGCTGACCCTGCCGGCGGCGATCACCC
TGGCCGGGCTGCTCTATGCCGGCCTGCGGTTCGTGTTCTGA

Upstream 100 bases:

>100_bases
TGGGTGGACGCCGCTGCGCAACCGGCCGCAGCAGGGTCCGAGACCGTACGGCCCCGCGCCCGGCATCAGCGAAGTCCGCA
GTCGATTCCGTCGAGGTTCA

Downstream 100 bases:

>100_bases
GGCGAAACGAGGGTCAAACGCCTGCGTACATCCGGCCGGGGTTGAAGATGCCGGCCGGATCATGCGCGGCCTTGATGCCG
GCGGTGATGCGCATCAGCGG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSDALSLQDGGSAPTEARSPRGPNLDGRMHPAGIGLFLLVLVGGLGYALFNLITDMQAAGQPPLAIGAFALLGLALLIAL
GFEFVNGFHDTANAVATVIYTHSLPPVVAVVYSGVFNFLGVLVSTGAVAYSVVTLLPVELVLKSGSSAGYAMIFALLIAA
IIWNLGTWALGLPNSSSHALIGSILGVGLANQLLAAEGSGTSGVQWNQASSVLRALLFSPLVGFVMAALLLLTMRFVIRN
RTLYQEPEGDTPPPLWIRALLILTCGGVSFAHGGNDGQKGMGLIMLILIGAAPTAYALNRTMSESATPHFVQVSERARTV
FQNRADQAPMPSPAEGRVQITEALGKKDLDRPETYAALAALSDDIATKVKEYGAIRHVPAEATPNLRNDMYLASDAIHSL
KKHEGLFVGEEAEMLKAYETSLNDGTRYIPTWVKVSVALALGLGTMVGWKRIVVTVGSRIGKKHLTYAQGASAEITAAGT
IGLAEAYGLPVSTTHILSSGVAGTMAANGSGLQWATVRNLAAAWLLTLPAAITLAGLLYAGLRFVF

Sequences:

>Translated_546_residues
MSDALSLQDGGSAPTEARSPRGPNLDGRMHPAGIGLFLLVLVGGLGYALFNLITDMQAAGQPPLAIGAFALLGLALLIAL
GFEFVNGFHDTANAVATVIYTHSLPPVVAVVYSGVFNFLGVLVSTGAVAYSVVTLLPVELVLKSGSSAGYAMIFALLIAA
IIWNLGTWALGLPNSSSHALIGSILGVGLANQLLAAEGSGTSGVQWNQASSVLRALLFSPLVGFVMAALLLLTMRFVIRN
RTLYQEPEGDTPPPLWIRALLILTCGGVSFAHGGNDGQKGMGLIMLILIGAAPTAYALNRTMSESATPHFVQVSERARTV
FQNRADQAPMPSPAEGRVQITEALGKKDLDRPETYAALAALSDDIATKVKEYGAIRHVPAEATPNLRNDMYLASDAIHSL
KKHEGLFVGEEAEMLKAYETSLNDGTRYIPTWVKVSVALALGLGTMVGWKRIVVTVGSRIGKKHLTYAQGASAEITAAGT
IGLAEAYGLPVSTTHILSSGVAGTMAANGSGLQWATVRNLAAAWLLTLPAAITLAGLLYAGLRFVF
>Mature_545_residues
SDALSLQDGGSAPTEARSPRGPNLDGRMHPAGIGLFLLVLVGGLGYALFNLITDMQAAGQPPLAIGAFALLGLALLIALG
FEFVNGFHDTANAVATVIYTHSLPPVVAVVYSGVFNFLGVLVSTGAVAYSVVTLLPVELVLKSGSSAGYAMIFALLIAAI
IWNLGTWALGLPNSSSHALIGSILGVGLANQLLAAEGSGTSGVQWNQASSVLRALLFSPLVGFVMAALLLLTMRFVIRNR
TLYQEPEGDTPPPLWIRALLILTCGGVSFAHGGNDGQKGMGLIMLILIGAAPTAYALNRTMSESATPHFVQVSERARTVF
QNRADQAPMPSPAEGRVQITEALGKKDLDRPETYAALAALSDDIATKVKEYGAIRHVPAEATPNLRNDMYLASDAIHSLK
KHEGLFVGEEAEMLKAYETSLNDGTRYIPTWVKVSVALALGLGTMVGWKRIVVTVGSRIGKKHLTYAQGASAEITAAGTI
GLAEAYGLPVSTTHILSSGVAGTMAANGSGLQWATVRNLAAAWLLTLPAAITLAGLLYAGLRFVF

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=477, Percent_Identity=42.5576519916143, Blast_Score=355, Evalue=5e-99,
Organism=Escherichia coli, GI1789360, Length=476, Percent_Identity=41.5966386554622, Blast_Score=350, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI25146401, Length=494, Percent_Identity=23.0769230769231, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 57111; Mature: 56980

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDALSLQDGGSAPTEARSPRGPNLDGRMHPAGIGLFLLVLVGGLGYALFNLITDMQAAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPPLAIGAFALLGLALLIALGFEFVNGFHDTANAVATVIYTHSLPPVVAVVYSGVFNFLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VLVSTGAVAYSVVTLLPVELVLKSGSSAGYAMIFALLIAAIIWNLGTWALGLPNSSSHAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHH
IGSILGVGLANQLLAAEGSGTSGVQWNQASSVLRALLFSPLVGFVMAALLLLTMRFVIRN
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RTLYQEPEGDTPPPLWIRALLILTCGGVSFAHGGNDGQKGMGLIMLILIGAAPTAYALNR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
TMSESATPHFVQVSERARTVFQNRADQAPMPSPAEGRVQITEALGKKDLDRPETYAALAA
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHH
LSDDIATKVKEYGAIRHVPAEATPNLRNDMYLASDAIHSLKKHEGLFVGEEAEMLKAYET
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCHHHHHHHHHH
SLNDGTRYIPTWVKVSVALALGLGTMVGWKRIVVTVGSRIGKKHLTYAQGASAEITAAGT
HCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHCCCCCEEEECCC
IGLAEAYGLPVSTTHILSSGVAGTMAANGSGLQWATVRNLAAAWLLTLPAAITLAGLLYA
HHHHHHCCCCCHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLRFVF
HHHHCC
>Mature Secondary Structure 
SDALSLQDGGSAPTEARSPRGPNLDGRMHPAGIGLFLLVLVGGLGYALFNLITDMQAAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPPLAIGAFALLGLALLIALGFEFVNGFHDTANAVATVIYTHSLPPVVAVVYSGVFNFLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VLVSTGAVAYSVVTLLPVELVLKSGSSAGYAMIFALLIAAIIWNLGTWALGLPNSSSHAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHH
IGSILGVGLANQLLAAEGSGTSGVQWNQASSVLRALLFSPLVGFVMAALLLLTMRFVIRN
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RTLYQEPEGDTPPPLWIRALLILTCGGVSFAHGGNDGQKGMGLIMLILIGAAPTAYALNR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
TMSESATPHFVQVSERARTVFQNRADQAPMPSPAEGRVQITEALGKKDLDRPETYAALAA
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHH
LSDDIATKVKEYGAIRHVPAEATPNLRNDMYLASDAIHSLKKHEGLFVGEEAEMLKAYET
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCHHHHHHHHHH
SLNDGTRYIPTWVKVSVALALGLGTMVGWKRIVVTVGSRIGKKHLTYAQGASAEITAAGT
HCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHCCCCCEEEECCC
IGLAEAYGLPVSTTHILSSGVAGTMAANGSGLQWATVRNLAAAWLLTLPAAITLAGLLYA
HHHHHHCCCCCHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLRFVF
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]