Click here to switch to the map view.

The map label for this gene is groL [H]

Identifier: 218533123

GI number: 218533123

Start: 5620326

End: 5621966

Strand: Reverse

Name: groL [H]

Synonym: Mchl_5249

Alternate gene names: 218533123

Gene position: 5621966-5620326 (Counterclockwise)

Preceding gene: 218533124

Following gene: 218533122

Centisome position: 97.3

GC content: 67.03

Gene sequence:

>1641_bases
ATGGCAGCGAAAGACGTACGTTTCTCCGCCGATGCTCGCGACAAGATGCTGCGCGGCGTCGACATCCTCGCGGATGCCGT
CAAGGTGACGCTCGGCCCCAAGGGCCGCAACGTCGTGATCGAGAAGAGCTTCGGCGCCCCGCGCATCACCAAGGACGGCG
TCACCGTCGCCAAGGAGATCGAGCTCGCCGACCGCTTCGAGAACATGGGCGCACAGATGGTGCGCGAAGTGGCCTCGAAG
ACCAACGACATCGCCGGTGACGGCACCACCACCGCGACCGTGCTGGCCCAGGCGATCGTCCGCGAAGGCGCCAAGTACGT
CGCCGCCGGCATCAACCCGATGGACCTGAAGCGCGGCATCGACCTCGCCACGGCCGCCGCGGTGAAGGACATCACCGCCC
GCGCCAAGAAGGTCGCCTCCTCCGAAGAGGTCGCCCAGGTCGGCACGATCTCCGCCAATGGCGACAAGGAGATCGGCGAG
ATGATCGCCCACGCCATGCAGAAGGTGGGCAACGAGGGCGTCATCACCGTCGAGGAGGCCAAGACCGCCGAGACCGAGCT
CGACGTCGTCGAGGGCATGCAGTTCGACCGCGGCTACCTCTCGCCGTACTTCGTGACCAACGCCGAGAAGATGGTCGCCG
AGCTCGAGGATCCCTACATCCTCATCCACGAGAAGAAGCTCTCCTCGCTGCAGCCGATGCTGCCGGTGCTCGAGGCCGTG
GTGCAGACCGGCAAGCCGCTCGTCATCATCGCCGAGGACATCGAGGGTGAGGCGCTCGCCACGCTCGTCGTGAACAAGCT
GCGCGGCGGCCTCAAGGTCGCGGCCGTGAAGGCTCCGGGCTTCGGTGATCGCCGCAAGGCGATGCTCGAGGACATCGCGA
TCCTCACCAAGGGCCAGACCATCTCCGAGGATCTCGGCATCAAGCTCGAGAACGTCGCCCTGCCGATGCTCGGCCGCGCC
AAGCGCGTCCGCATCGAGAAGGAGACCACCACGATCATCGACGGTCTCGGCGAGAAGGCCGACATCGAGGCCCGCGTCGG
CCAGATCAAGGCGCAGATCGAGGAGACCACCTCGGACTACGATCGTGAGAAGCTCCAGGAGCGTCTGGCCAAGCTCGCGG
GCGGCGTCGCGGTGATCCGCGTCGGCGGCGCGACCGAGGTCGAGGTCAAGGAGAAGAAGGATCGCGTGGACGACGCGCTC
AACGCCACCCGCGCTGCGGTGGAAGAGGGCATCGTCCCCGGCGGCGGCGTCGCTCTGCTCCTGGCCAAGAAGGCGGTCGC
CGAGCTGAAGTCCGACATCCCGGACGTCCAGGCCGGCATCAAGATCGTCCTCAAGGCCCTCGAAGCCCCGATCCGTCAGA
TCGCCAGCAACGCGGGTGTCGAGGGCTCCATCGTCGTCGGCAAGATCACCGACAACGGCGGCGAGACCTTCGGCTTCAAC
GCGCAGACCGAAGAGTATGTCGACATGATCCAGGCCGGCATCGTCGACCCGGCCAAGGTCGTGCGTACCGCCCTTCAGGA
CGCGGCCTCGGTCGCCGGCCTGCTGGTGACCACGGAAGCCATGGTCGCCGACGCGCCGAAGAAGGATAGCGGCGCCCCGG
CGATGCCGGGCGGCGGCGGCATGGGCGGCATGGACTTCTAA

Upstream 100 bases:

>100_bases
CCCTCTAAGGCGCGCAACCCCTCCACCGCCCTTCGAGGCCGCCAGGGCTTTTGCCCTCGGCCGGCTTCAGGGCCCCTTCC
ATCATTCGAGGTAAACTCAC

Downstream 100 bases:

>100_bases
GTCCACGCACCCGGTCGAAGAACGGAAGGGGGGTCGCCGAAAGGCGGCCCCTTTTTCGTCGGTGGCACCGCGGGGCGCCG
ACGATGAGGGACGTGCGCGG

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MAAKDVRFSADARDKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELADRFENMGAQMVREVASK
TNDIAGDGTTTATVLAQAIVREGAKYVAAGINPMDLKRGIDLATAAAVKDITARAKKVASSEEVAQVGTISANGDKEIGE
MIAHAMQKVGNEGVITVEEAKTAETELDVVEGMQFDRGYLSPYFVTNAEKMVAELEDPYILIHEKKLSSLQPMLPVLEAV
VQTGKPLVIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAILTKGQTISEDLGIKLENVALPMLGRA
KRVRIEKETTTIIDGLGEKADIEARVGQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDAL
NATRAAVEEGIVPGGGVALLLAKKAVAELKSDIPDVQAGIKIVLKALEAPIRQIASNAGVEGSIVVGKITDNGGETFGFN
AQTEEYVDMIQAGIVDPAKVVRTALQDAASVAGLLVTTEAMVADAPKKDSGAPAMPGGGGMGGMDF

Sequences:

>Translated_546_residues
MAAKDVRFSADARDKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELADRFENMGAQMVREVASK
TNDIAGDGTTTATVLAQAIVREGAKYVAAGINPMDLKRGIDLATAAAVKDITARAKKVASSEEVAQVGTISANGDKEIGE
MIAHAMQKVGNEGVITVEEAKTAETELDVVEGMQFDRGYLSPYFVTNAEKMVAELEDPYILIHEKKLSSLQPMLPVLEAV
VQTGKPLVIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAILTKGQTISEDLGIKLENVALPMLGRA
KRVRIEKETTTIIDGLGEKADIEARVGQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDAL
NATRAAVEEGIVPGGGVALLLAKKAVAELKSDIPDVQAGIKIVLKALEAPIRQIASNAGVEGSIVVGKITDNGGETFGFN
AQTEEYVDMIQAGIVDPAKVVRTALQDAASVAGLLVTTEAMVADAPKKDSGAPAMPGGGGMGGMDF
>Mature_545_residues
AAKDVRFSADARDKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELADRFENMGAQMVREVASKT
NDIAGDGTTTATVLAQAIVREGAKYVAAGINPMDLKRGIDLATAAAVKDITARAKKVASSEEVAQVGTISANGDKEIGEM
IAHAMQKVGNEGVITVEEAKTAETELDVVEGMQFDRGYLSPYFVTNAEKMVAELEDPYILIHEKKLSSLQPMLPVLEAVV
QTGKPLVIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAILTKGQTISEDLGIKLENVALPMLGRAK
RVRIEKETTTIIDGLGEKADIEARVGQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALN
ATRAAVEEGIVPGGGVALLLAKKAVAELKSDIPDVQAGIKIVLKALEAPIRQIASNAGVEGSIVVGKITDNGGETFGFNA
QTEEYVDMIQAGIVDPAKVVRTALQDAASVAGLLVTTEAMVADAPKKDSGAPAMPGGGGMGGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=528, Percent_Identity=54.3560606060606, Blast_Score=557, Evalue=1e-159,
Organism=Homo sapiens, GI31542947, Length=528, Percent_Identity=54.3560606060606, Blast_Score=557, Evalue=1e-159,
Organism=Homo sapiens, GI5453607, Length=548, Percent_Identity=24.4525547445255, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI261399877, Length=520, Percent_Identity=24.0384615384615, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI261399875, Length=481, Percent_Identity=24.3243243243243, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790586, Length=530, Percent_Identity=67.3584905660377, Blast_Score=694, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=52.930056710775, Blast_Score=550, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI193210679, Length=211, Percent_Identity=54.0284360189574, Blast_Score=222, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=55.5133079847909, Blast_Score=579, Evalue=1e-166,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=55.4924242424242, Blast_Score=571, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=55.4924242424242, Blast_Score=571, Evalue=1e-163,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=52.7619047619048, Blast_Score=538, Evalue=1e-153,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=52.7619047619048, Blast_Score=538, Evalue=1e-153,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=52.7619047619048, Blast_Score=538, Evalue=1e-153,
Organism=Drosophila melanogaster, GI17864606, Length=549, Percent_Identity=46.448087431694, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24584129, Length=537, Percent_Identity=36.4990689013035, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI19921262, Length=537, Percent_Identity=36.4990689013035, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57469; Mature: 57338

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDVRFSADARDKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCCHHHHHH
ELADRFENMGAQMVREVASKTNDIAGDGTTTATVLAQAIVREGAKYVAAGINPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHCC
DLATAAAVKDITARAKKVASSEEVAQVGTISANGDKEIGEMIAHAMQKVGNEGVITVEEA
CHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELDVVEGMQFDRGYLSPYFVTNAEKMVAELEDPYILIHEKKLSSLQPMLPVLEAV
CCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHH
VQTGKPLVIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAILTKGQT
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCC
ISEDLGIKLENVALPMLGRAKRVRIEKETTTIIDGLGEKADIEARVGQIKAQIEETTSDY
CHHHHCEEEEEECCCCCCCCHHEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALNATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LAKKAVAELKSDIPDVQAGIKIVLKALEAPIRQIASNAGVEGSIVVGKITDNGGETFGFN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCC
AQTEEYVDMIQAGIVDPAKVVRTALQDAASVAGLLVTTEAMVADAPKKDSGAPAMPGGGG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MGGMDF
CCCCCC
>Mature Secondary Structure 
AAKDVRFSADARDKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEI
CCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCCHHHHHH
ELADRFENMGAQMVREVASKTNDIAGDGTTTATVLAQAIVREGAKYVAAGINPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHCC
DLATAAAVKDITARAKKVASSEEVAQVGTISANGDKEIGEMIAHAMQKVGNEGVITVEEA
CHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELDVVEGMQFDRGYLSPYFVTNAEKMVAELEDPYILIHEKKLSSLQPMLPVLEAV
CCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHH
VQTGKPLVIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAILTKGQT
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCC
ISEDLGIKLENVALPMLGRAKRVRIEKETTTIIDGLGEKADIEARVGQIKAQIEETTSDY
CHHHHCEEEEEECCCCCCCCHHEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALNATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LAKKAVAELKSDIPDVQAGIKIVLKALEAPIRQIASNAGVEGSIVVGKITDNGGETFGFN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCC
AQTEEYVDMIQAGIVDPAKVVRTALQDAASVAGLLVTTEAMVADAPKKDSGAPAMPGGGG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MGGMDF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA