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The map label for this gene is lexA [H]

Identifier: 218533024

GI number: 218533024

Start: 5514244

End: 5514966

Strand: Reverse

Name: lexA [H]

Synonym: Mchl_5148

Alternate gene names: 218533024

Gene position: 5514966-5514244 (Counterclockwise)

Preceding gene: 218533025

Following gene: 218533022

Centisome position: 95.45

GC content: 65.98

Gene sequence:

>723_bases
ATGCTGACCCGCAAGCAACTCGAACTGCTCCAGTTCATCCAGCAGAGGATGCAGGAGAGCGGTGTGCCGCCGTCCTTCGA
CGAGATGAAGGACGCGCTCGACCTGAAATCCAAATCCGGCATCCACCGCCTGATCATGGCGCTGGAAGAGCGCGGGTTTC
TTCGCCGTCTGCCGAACCGGGCGCGGGCGATCGAAATTCTGCGCATGCCCGACATGCCGGCGGCCAAGCCCGCTTCGTCC
GAGCCGCGCCGCTTCACGCCGAGCGTGGTCGAGGGGGGCCTTTCGGCCAAGCCCGCCGCGCCGAAGCCTCCCATGCTTCA
GGCCCATGATGGCAAGGGCCAATCGGTGATGGTGCCGGTGATGGGCCGGATCGCTGCCGGTGTGCCGATCTCGGCGATCG
AGAGCCAGAGCCACTCGATTTCGATGTCCCCCGACTTCCTGTCGGGCGGAGAGCATTACGCGCTGGAAGTGCGCGGCGAT
TCGATGATCGAGGCCGGCATTCTCGATGGCGACCTCGTGGTGATCCACAAACAGGAGACCGCCAACAATGGCGACATCAT
CGTCGCGCTGATCGACGACGAGGAGGCGACCCTCAAGCGCCTTCGCCGCCGCGGCTCGTCGATCGCGCTGGAAGCCGCCA
ACCCGGCCTACGAGACCCGCGTGCTCGGGCCCGACCGGGTGCGCATCCAGGGCAAGCTCGTCAGCCTCGTGCGCCGTTAC
TGA

Upstream 100 bases:

>100_bases
AGGGATGCTTGCGGAACAGGAGAGGAACGTCTAGGCTGTTCGTGCTTTGTTTTCAACGATTCTGCGGCGCCCCCGGCCCG
CGGTCGACAGCAGGTGCCGC

Downstream 100 bases:

>100_bases
GGATTTTCGGAGGGCGGCGGGTCTGATGGATCCGCCGCACTTGCATCGTTGGGAGATTGGGGGCCCACCTCGGTTGCCGG
CGTCGCCACGCTGGCAGGCT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MLTRKQLELLQFIQQRMQESGVPPSFDEMKDALDLKSKSGIHRLIMALEERGFLRRLPNRARAIEILRMPDMPAAKPASS
EPRRFTPSVVEGGLSAKPAAPKPPMLQAHDGKGQSVMVPVMGRIAAGVPISAIESQSHSISMSPDFLSGGEHYALEVRGD
SMIEAGILDGDLVVIHKQETANNGDIIVALIDDEEATLKRLRRRGSSIALEAANPAYETRVLGPDRVRIQGKLVSLVRRY

Sequences:

>Translated_240_residues
MLTRKQLELLQFIQQRMQESGVPPSFDEMKDALDLKSKSGIHRLIMALEERGFLRRLPNRARAIEILRMPDMPAAKPASS
EPRRFTPSVVEGGLSAKPAAPKPPMLQAHDGKGQSVMVPVMGRIAAGVPISAIESQSHSISMSPDFLSGGEHYALEVRGD
SMIEAGILDGDLVVIHKQETANNGDIIVALIDDEEATLKRLRRRGSSIALEAANPAYETRVLGPDRVRIQGKLVSLVRRY
>Mature_240_residues
MLTRKQLELLQFIQQRMQESGVPPSFDEMKDALDLKSKSGIHRLIMALEERGFLRRLPNRARAIEILRMPDMPAAKPASS
EPRRFTPSVVEGGLSAKPAAPKPPMLQAHDGKGQSVMVPVMGRIAAGVPISAIESQSHSISMSPDFLSGGEHYALEVRGD
SMIEAGILDGDLVVIHKQETANNGDIIVALIDDEEATLKRLRRRGSSIALEAANPAYETRVLGPDRVRIQGKLVSLVRRY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=239, Percent_Identity=25.9414225941423, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 26334; Mature: 26334

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQLELLQFIQQRMQESGVPPSFDEMKDALDLKSKSGIHRLIMALEERGFLRRLPNR
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCH
ARAIEILRMPDMPAAKPASSEPRRFTPSVVEGGLSAKPAAPKPPMLQAHDGKGQSVMVPV
HHEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEEEH
MGRIAAGVPISAIESQSHSISMSPDFLSGGEHYALEVRGDSMIEAGILDGDLVVIHKQET
HHHHHCCCCHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCEEEEECCCC
ANNGDIIVALIDDEEATLKRLRRRGSSIALEAANPAYETRVLGPDRVRIQGKLVSLVRRY
CCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCCCHHEEECCCCCEEEHHHHHHHHHCC
>Mature Secondary Structure
MLTRKQLELLQFIQQRMQESGVPPSFDEMKDALDLKSKSGIHRLIMALEERGFLRRLPNR
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCH
ARAIEILRMPDMPAAKPASSEPRRFTPSVVEGGLSAKPAAPKPPMLQAHDGKGQSVMVPV
HHEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEEEH
MGRIAAGVPISAIESQSHSISMSPDFLSGGEHYALEVRGDSMIEAGILDGDLVVIHKQET
HHHHHCCCCHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCEEEEECCCC
ANNGDIIVALIDDEEATLKRLRRRGSSIALEAANPAYETRVLGPDRVRIQGKLVSLVRRY
CCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCCCHHEEECCCCCEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA