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The map label for this gene is metG [H]

Identifier: 218532446

GI number: 218532446

Start: 4826381

End: 4827949

Strand: Reverse

Name: metG [H]

Synonym: Mchl_4558

Alternate gene names: 218532446

Gene position: 4827949-4826381 (Counterclockwise)

Preceding gene: 218532447

Following gene: 218532445

Centisome position: 83.56

GC content: 67.75

Gene sequence:

>1569_bases
GTGAGCGCGAGCGAGAAGGCGAACGAGCCCTTCCTCATCACCACGGCGATTTCTTACCCGAACGGCGCGCCGCATATCGG
CCACGCCTACGAGGTGATCGCCACCGACGCCATCGCCCGCTTCCACCGCCTCGACGGGCGCGACGTGCTGTTCACCACCG
GCACCGATGAGCACGGCCTCAAGATCCAGCAGACGGCGTCCCGCGCCGGCGTCACGCCGCGCGCCTTCGTCGATGACATG
GCCGGGCGCTTCAAGGCCGTGGCCGACCGGCTCGACTGCAGCTACGACCGCTTCATCCGCACTACGGAAGCCGACCACTA
CGCCGCGGCCCAGGAGCTGTGGCGGCGGATGGAGGCCAACGGCGACATCTACCTCGCCAAATATGCCGGCTGGTACTCGG
TGCGCGACGAGGCCTATTACGACGAGGCCGAGACCACGCTCGGCCCCGACGGCAACCGCCGCTCGATCAAGACGGACACG
CCCGTCGAGTGGATGGAGGAGGAAAACTACCTCTTCCGTCTCTCGAAGTACCAGGAACCCCTGCTCAAGCTCTACGCGGA
GCAGCCGGACTTCCTCGGCCCCGAGACGCGGATGAACGAGGTCGCGAGCTTCGTGCGCTCCGGCCTCAAGGATCTCTCGA
TCAGCCGCACCACCTTCGACTGGGGCGTGCCGGTGCCGGACCGGCCGGGCCACGTCATGTATGTCTGGGTCGATGCCCTG
ACCAATTACCTGACGGTGACGGGCTTTCCCGATGCGCAGGCGCCGAACGCCCGGTTCTGGCCGGCGAGCCTGCACATCAT
CGGCAAGGACATCGTCCGCTTCCACGCGGTCTACTGGCCGGCCTTCCTGATGTCGGCCGGGCTGCCGCTGCCCAAGCGCG
TGTTCGGCCACGGCTTCTTTCTCAGCCGCGGCGAGAAGATGTCGAAGTCGCTCGGCAACGTGGTCGATCCGCTCGATCTC
GTCGGCACCTATGGCGTCGATCCGGTGCGCTACTTCCTCCTGCGCGAGGCGCCATTCGGCAGCGACGGCAATTACGACCA
CGAGGCGATCATCAACCGCATCAACGCGGACCTCGCCAACGACCTCGGCAACCTCGCCCAGCGCTCGCTCTCGATGATCA
CGAAGAACTGTGAGGGCGCGGTGCCGGAGCCGGGTGCCTTCACCGAGGCCGACGAACGCCTGCTCGCCGCCGCCGCCGCC
CTGCCGGAGAAGGCACGGGGGCTGATGCAGAATCTCGCCCTGCACGCGATCCTGGCCGAGATCTGGGCCGTGGTCGGCGA
GGCCAATCGCTACTTCGCCTCCGAAGAGCCTTGGAAACTGCGCAAGTCCGATCCGGCGCGCATGAACACGGTGCTCTACG
TCACGGTCGAGACCCTGCGCCGGGTCGGCCTGATGGTGCAGCCCTTCGTGCCGACGGCGGGCGCGGCCTTGCTCGACCTG
CTCGCCGTGCCGGTGAATGCACGCAGCTTCGCTTTTACCGGCGCCGAGCACAGCCTGCAGGGCGGCACGGCGCTGCCCGC
CCCGGCCCCGATCTTCCCCCGCTTCGAGAAGCCGGAGGCGACGGCCTGA

Upstream 100 bases:

>100_bases
GCGCGCCGGCCTCCGGCGCCCGGCAGGCCGGTTGCGTCGCGGGACGTTGCGCGCCACAAGGCATGACAGTCCGACCTTCG
CATCCGGGACATCTTCTGAC

Downstream 100 bases:

>100_bases
CGCCGCCTCCCCGCATCGCTCCTTCCCCAAAGAATCCGCCAAAGAGACCATGCTGGTCGACAGCCACTGCCACCTCGATT
TCCCGGACTTCGCGCAGGAC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSASEKANEPFLITTAISYPNGAPHIGHAYEVIATDAIARFHRLDGRDVLFTTGTDEHGLKIQQTASRAGVTPRAFVDDM
AGRFKAVADRLDCSYDRFIRTTEADHYAAAQELWRRMEANGDIYLAKYAGWYSVRDEAYYDEAETTLGPDGNRRSIKTDT
PVEWMEEENYLFRLSKYQEPLLKLYAEQPDFLGPETRMNEVASFVRSGLKDLSISRTTFDWGVPVPDRPGHVMYVWVDAL
TNYLTVTGFPDAQAPNARFWPASLHIIGKDIVRFHAVYWPAFLMSAGLPLPKRVFGHGFFLSRGEKMSKSLGNVVDPLDL
VGTYGVDPVRYFLLREAPFGSDGNYDHEAIINRINADLANDLGNLAQRSLSMITKNCEGAVPEPGAFTEADERLLAAAAA
LPEKARGLMQNLALHAILAEIWAVVGEANRYFASEEPWKLRKSDPARMNTVLYVTVETLRRVGLMVQPFVPTAGAALLDL
LAVPVNARSFAFTGAEHSLQGGTALPAPAPIFPRFEKPEATA

Sequences:

>Translated_522_residues
MSASEKANEPFLITTAISYPNGAPHIGHAYEVIATDAIARFHRLDGRDVLFTTGTDEHGLKIQQTASRAGVTPRAFVDDM
AGRFKAVADRLDCSYDRFIRTTEADHYAAAQELWRRMEANGDIYLAKYAGWYSVRDEAYYDEAETTLGPDGNRRSIKTDT
PVEWMEEENYLFRLSKYQEPLLKLYAEQPDFLGPETRMNEVASFVRSGLKDLSISRTTFDWGVPVPDRPGHVMYVWVDAL
TNYLTVTGFPDAQAPNARFWPASLHIIGKDIVRFHAVYWPAFLMSAGLPLPKRVFGHGFFLSRGEKMSKSLGNVVDPLDL
VGTYGVDPVRYFLLREAPFGSDGNYDHEAIINRINADLANDLGNLAQRSLSMITKNCEGAVPEPGAFTEADERLLAAAAA
LPEKARGLMQNLALHAILAEIWAVVGEANRYFASEEPWKLRKSDPARMNTVLYVTVETLRRVGLMVQPFVPTAGAALLDL
LAVPVNARSFAFTGAEHSLQGGTALPAPAPIFPRFEKPEATA
>Mature_521_residues
SASEKANEPFLITTAISYPNGAPHIGHAYEVIATDAIARFHRLDGRDVLFTTGTDEHGLKIQQTASRAGVTPRAFVDDMA
GRFKAVADRLDCSYDRFIRTTEADHYAAAQELWRRMEANGDIYLAKYAGWYSVRDEAYYDEAETTLGPDGNRRSIKTDTP
VEWMEEENYLFRLSKYQEPLLKLYAEQPDFLGPETRMNEVASFVRSGLKDLSISRTTFDWGVPVPDRPGHVMYVWVDALT
NYLTVTGFPDAQAPNARFWPASLHIIGKDIVRFHAVYWPAFLMSAGLPLPKRVFGHGFFLSRGEKMSKSLGNVVDPLDLV
GTYGVDPVRYFLLREAPFGSDGNYDHEAIINRINADLANDLGNLAQRSLSMITKNCEGAVPEPGAFTEADERLLAAAAAL
PEKARGLMQNLALHAILAEIWAVVGEANRYFASEEPWKLRKSDPARMNTVLYVTVETLRRVGLMVQPFVPTAGAALLDLL
AVPVNARSFAFTGAEHSLQGGTALPAPAPIFPRFEKPEATA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=538, Percent_Identity=38.2899628252788, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI14043022, Length=556, Percent_Identity=26.0791366906475, Blast_Score=155, Evalue=7e-38,
Organism=Escherichia coli, GI1788432, Length=499, Percent_Identity=25.8517034068136, Blast_Score=173, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI212645096, Length=492, Percent_Identity=38.2113821138211, Blast_Score=349, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI71992781, Length=386, Percent_Identity=37.3056994818653, Blast_Score=264, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17540794, Length=556, Percent_Identity=24.4604316546763, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321610, Length=526, Percent_Identity=33.8403041825095, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6321704, Length=536, Percent_Identity=24.2537313432836, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21358483, Length=504, Percent_Identity=38.0952380952381, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24655311, Length=563, Percent_Identity=25.9325044404973, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI21355409, Length=179, Percent_Identity=26.2569832402235, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57883; Mature: 57752

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASEKANEPFLITTAISYPNGAPHIGHAYEVIATDAIARFHRLDGRDVLFTTGTDEHGL
CCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCE
KIQQTASRAGVTPRAFVDDMAGRFKAVADRLDCSYDRFIRTTEADHYAAAQELWRRMEAN
EEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCC
GDIYLAKYAGWYSVRDEAYYDEAETTLGPDGNRRSIKTDTPVEWMEEENYLFRLSKYQEP
CCEEEEEECCEEEECCHHHHCCHHCCCCCCCCCCCCCCCCCHHHHCCCCCEEEHHHHHHH
LLKLYAEQPDFLGPETRMNEVASFVRSGLKDLSISRTTFDWGVPVPDRPGHVMYVWVDAL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEHHHH
TNYLTVTGFPDAQAPNARFWPASLHIIGKDIVRFHAVYWPAFLMSAGLPLPKRVFGHGFF
HHHEEEECCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHH
LSRGEKMSKSLGNVVDPLDLVGTYGVDPVRYFLLREAPFGSDGNYDHEAIINRINADLAN
HHCCHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
DLGNLAQRSLSMITKNCEGAVPEPGAFTEADERLLAAAAALPEKARGLMQNLALHAILAE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IWAVVGEANRYFASEEPWKLRKSDPARMNTVLYVTVETLRRVGLMVQPFVPTAGAALLDL
HHHHHHCHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCEEECCCCCHHHHHHHH
LAVPVNARSFAFTGAEHSLQGGTALPAPAPIFPRFEKPEATA
HHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SASEKANEPFLITTAISYPNGAPHIGHAYEVIATDAIARFHRLDGRDVLFTTGTDEHGL
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCE
KIQQTASRAGVTPRAFVDDMAGRFKAVADRLDCSYDRFIRTTEADHYAAAQELWRRMEAN
EEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCC
GDIYLAKYAGWYSVRDEAYYDEAETTLGPDGNRRSIKTDTPVEWMEEENYLFRLSKYQEP
CCEEEEEECCEEEECCHHHHCCHHCCCCCCCCCCCCCCCCCHHHHCCCCCEEEHHHHHHH
LLKLYAEQPDFLGPETRMNEVASFVRSGLKDLSISRTTFDWGVPVPDRPGHVMYVWVDAL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEHHHH
TNYLTVTGFPDAQAPNARFWPASLHIIGKDIVRFHAVYWPAFLMSAGLPLPKRVFGHGFF
HHHEEEECCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHH
LSRGEKMSKSLGNVVDPLDLVGTYGVDPVRYFLLREAPFGSDGNYDHEAIINRINADLAN
HHCCHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
DLGNLAQRSLSMITKNCEGAVPEPGAFTEADERLLAAAAALPEKARGLMQNLALHAILAE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IWAVVGEANRYFASEEPWKLRKSDPARMNTVLYVTVETLRRVGLMVQPFVPTAGAALLDL
HHHHHHCHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCEEECCCCCHHHHHHHH
LAVPVNARSFAFTGAEHSLQGGTALPAPAPIFPRFEKPEATA
HHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]