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The map label for this gene is flbD [H]

Identifier: 218532344

GI number: 218532344

Start: 4715869

End: 4717242

Strand: Reverse

Name: flbD [H]

Synonym: Mchl_4456

Alternate gene names: 218532344

Gene position: 4717242-4715869 (Counterclockwise)

Preceding gene: 218532345

Following gene: 218532340

Centisome position: 81.64

GC content: 71.47

Gene sequence:

>1374_bases
ATGCGGCTGCTCATCGTCGGACGGCTCTCGAGCGAACTCGTCACCGCCTCGAAGATCGCCATGAACCGGGGCGCCTCGGT
CACCCACGCCGACGGGATCGACCAGGGGCTCGCGACCCTGCGCGCCAAGGGCGGCGACCTCGTCATGGTCGATGTCGGGC
TCGACATCCGCCGGCTGGTGCAGGCCCTGTCCGACGAGCGCATCCGCACGCCGGTGGTGGCCTGCGGCATCGCCTCCGAC
GCCCGCGCCGCGGTGGCGGCGATCCAGGCGGGCGCCAAGGAGTACATCCCCCTCCCCCCCGACCCCGAACTGATCGCGGC
AGTCCTGGAGGCCGTGGCGGCGGACGCCCGCAGCTTCATCTGGCGCGATCCGTCCATGGAGCGCGTGGTGCGGCTCGCCG
AGCAGGTCGCCCGCTCGGAAGCCTCCGTGCTCATCACCGGCGAGAGCGGCACCGGCAAGGAGGTGCTGGCCCGCCACGTC
CACGCCAAGTCGAACCGGGCGGGCCGCCCGTTCGTCTCGGTCAACTGCGCGGCGATCCCCGAAGCCCTGCTCGAATCCGA
GCTGTTCGGCCACGAGAAGGGCGCCTTCACCGGCGCGGTCGCCCGGCGCATCGGCCGGTTCGAGGAGGCCAATGGCGGCA
CGCTGCTCCTCGACGAGATCTCCGAGATGGACGTGCGGCTGCAATCCAAGCTGCTGCGCGCGCTGCAGGAGCGGGTGATC
GACCGGGTTGGCGGAGCCGCCCCCGTCAAGGTGGATATCCGCGTGCTCGCCACCTCCAACCGTAACCTCGCCGACGAGGT
GAAGAAGGGCACGTTCCGCGAGGATCTGTACTACCGCCTCAACGTCGTGCATCTGCGCCTGCCTGCCTTGCGCGAGCGGC
TGGCCGACATCCTCGAACTCACCGCCCACTTCGCCCGCAAATACGCGGAGCTGAACGGGCTGCCGCTGCGTCCGCTGGCC
CGCGAGGCGCAGGCTCTGGTCGCCCGCAACCCCTGGCCCGGCAACGTGCGCGAGTTGGAGAACACGATCCACCGCGCGGT
GCTCCTGGCGCAGGGGGCCGAGATCGGTCCGGAGGCGATCCTGAGCCCGGAGGGCGAATCACTCGCGGCACCGACGGCGG
CCTCCGGCCCGGTCGAGCGGGCGGCGAGCGCAGCGGAGGCGGCGACCCGCGGGCTCGTCGGACGCACCGTGGCGGATGTC
GAGCGCGACCTGATCCTCGACACCCTCGACCATTGCCTCGGCAATCGCACCCACGCGGCGCGCATCCTCGGCATCTCGAT
CCGGACGCTGCGCAACAAGCTCAACGAGTATGTCGAGGCGGGCGTGAAGGTGGCCGAGCCCGGCGGCGTCCGGGCCATGG
CGGCCTATGGCTGA

Upstream 100 bases:

>100_bases
CGGCCACCGCCCCGCCTTCGCGGGGGCCGGCCTGCGATGAGAGATCATAGCGGGCCGGGACGGCCATCCGAACCGGATGA
ATTTTCGGAGACACCACGCC

Downstream 100 bases:

>100_bases
GCTCGGCGGCGTCGAAAGACGTCGGGCCGATTCTACGGAGTCAGCACGGTCGGAGCGGTGACGGCAGGCAGCACCGCCGC
ACCACGCATCCGCGCGACCA

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRLLIVGRLSSELVTASKIAMNRGASVTHADGIDQGLATLRAKGGDLVMVDVGLDIRRLVQALSDERIRTPVVACGIASD
ARAAVAAIQAGAKEYIPLPPDPELIAAVLEAVAADARSFIWRDPSMERVVRLAEQVARSEASVLITGESGTGKEVLARHV
HAKSNRAGRPFVSVNCAAIPEALLESELFGHEKGAFTGAVARRIGRFEEANGGTLLLDEISEMDVRLQSKLLRALQERVI
DRVGGAAPVKVDIRVLATSNRNLADEVKKGTFREDLYYRLNVVHLRLPALRERLADILELTAHFARKYAELNGLPLRPLA
REAQALVARNPWPGNVRELENTIHRAVLLAQGAEIGPEAILSPEGESLAAPTAASGPVERAASAAEAATRGLVGRTVADV
ERDLILDTLDHCLGNRTHAARILGISIRTLRNKLNEYVEAGVKVAEPGGVRAMAAYG

Sequences:

>Translated_457_residues
MRLLIVGRLSSELVTASKIAMNRGASVTHADGIDQGLATLRAKGGDLVMVDVGLDIRRLVQALSDERIRTPVVACGIASD
ARAAVAAIQAGAKEYIPLPPDPELIAAVLEAVAADARSFIWRDPSMERVVRLAEQVARSEASVLITGESGTGKEVLARHV
HAKSNRAGRPFVSVNCAAIPEALLESELFGHEKGAFTGAVARRIGRFEEANGGTLLLDEISEMDVRLQSKLLRALQERVI
DRVGGAAPVKVDIRVLATSNRNLADEVKKGTFREDLYYRLNVVHLRLPALRERLADILELTAHFARKYAELNGLPLRPLA
REAQALVARNPWPGNVRELENTIHRAVLLAQGAEIGPEAILSPEGESLAAPTAASGPVERAASAAEAATRGLVGRTVADV
ERDLILDTLDHCLGNRTHAARILGISIRTLRNKLNEYVEAGVKVAEPGGVRAMAAYG
>Mature_457_residues
MRLLIVGRLSSELVTASKIAMNRGASVTHADGIDQGLATLRAKGGDLVMVDVGLDIRRLVQALSDERIRTPVVACGIASD
ARAAVAAIQAGAKEYIPLPPDPELIAAVLEAVAADARSFIWRDPSMERVVRLAEQVARSEASVLITGESGTGKEVLARHV
HAKSNRAGRPFVSVNCAAIPEALLESELFGHEKGAFTGAVARRIGRFEEANGGTLLLDEISEMDVRLQSKLLRALQERVI
DRVGGAAPVKVDIRVLATSNRNLADEVKKGTFREDLYYRLNVVHLRLPALRERLADILELTAHFARKYAELNGLPLRPLA
REAQALVARNPWPGNVRELENTIHRAVLLAQGAEIGPEAILSPEGESLAAPTAASGPVERAASAAEAATRGLVGRTVADV
ERDLILDTLDHCLGNRTHAARILGISIRTLRNKLNEYVEAGVKVAEPGGVRAMAAYG

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=414, Percent_Identity=43.719806763285, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1790437, Length=433, Percent_Identity=41.108545034642, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1790299, Length=439, Percent_Identity=39.8633257403189, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1788905, Length=430, Percent_Identity=37.6744186046512, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI87082117, Length=237, Percent_Identity=51.4767932489451, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1787583, Length=314, Percent_Identity=42.6751592356688, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1789087, Length=269, Percent_Identity=46.4684014869888, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082152, Length=294, Percent_Identity=45.578231292517, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=40.3785488958991, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1786524, Length=329, Percent_Identity=41.0334346504559, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87081872, Length=340, Percent_Identity=35.8823529411765, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=38.5245901639344, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87081858, Length=324, Percent_Identity=29.9382716049383, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48971; Mature: 48971

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLIVGRLSSELVTASKIAMNRGASVTHADGIDQGLATLRAKGGDLVMVDVGLDIRRLV
CEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHH
QALSDERIRTPVVACGIASDARAAVAAIQAGAKEYIPLPPDPELIAAVLEAVAADARSFI
HHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
WRDPSMERVVRLAEQVARSEASVLITGESGTGKEVLARHVHAKSNRAGRPFVSVNCAAIP
CCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHH
EALLESELFGHEKGAFTGAVARRIGRFEEANGGTLLLDEISEMDVRLQSKLLRALQERVI
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
DRVGGAAPVKVDIRVLATSNRNLADEVKKGTFREDLYYRLNVVHLRLPALRERLADILEL
HHHCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHEEEEEEEECHHHHHHHHHHHHH
TAHFARKYAELNGLPLRPLAREAQALVARNPWPGNVRELENTIHRAVLLAQGAEIGPEAI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHC
LSPEGESLAAPTAASGPVERAASAAEAATRGLVGRTVADVERDLILDTLDHCLGNRTHAA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RILGISIRTLRNKLNEYVEAGVKVAEPGGVRAMAAYG
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCC
>Mature Secondary Structure
MRLLIVGRLSSELVTASKIAMNRGASVTHADGIDQGLATLRAKGGDLVMVDVGLDIRRLV
CEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHH
QALSDERIRTPVVACGIASDARAAVAAIQAGAKEYIPLPPDPELIAAVLEAVAADARSFI
HHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
WRDPSMERVVRLAEQVARSEASVLITGESGTGKEVLARHVHAKSNRAGRPFVSVNCAAIP
CCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHH
EALLESELFGHEKGAFTGAVARRIGRFEEANGGTLLLDEISEMDVRLQSKLLRALQERVI
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
DRVGGAAPVKVDIRVLATSNRNLADEVKKGTFREDLYYRLNVVHLRLPALRERLADILEL
HHHCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHEEEEEEEECHHHHHHHHHHHHH
TAHFARKYAELNGLPLRPLAREAQALVARNPWPGNVRELENTIHRAVLLAQGAEIGPEAI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHC
LSPEGESLAAPTAASGPVERAASAAEAATRGLVGRTVADVERDLILDTLDHCLGNRTHAA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RILGISIRTLRNKLNEYVEAGVKVAEPGGVRAMAAYG
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]