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The map label for this gene is ybdK [C]

Identifier: 218532303

GI number: 218532303

Start: 4671861

End: 4672985

Strand: Reverse

Name: ybdK [C]

Synonym: Mchl_4415

Alternate gene names: 218532303

Gene position: 4672985-4671861 (Counterclockwise)

Preceding gene: 218532309

Following gene: 218532298

Centisome position: 80.88

GC content: 72.18

Gene sequence:

>1125_bases
ATGAGCGCCCACCCCTACCGGTTCGGGATCGAGGAAGAGTATTTCCTCGCCGATGCCGAGACCCGCGGCACGCCGCGCCG
CTCGGTCAAGCCGTTCCATCAGGCGGCCGGCGAGCGGCTGCCCGAGATCGGGCGCGAGCTGCTGCAATGCCAGGTCGAGG
TCTGCACCCCGCCCTCCACGGCGTTCCCGGCCGCCCACGCCGCGCTGCGGGAGCAGCGGCAGGCCTTGGCGGAGCTGGGC
CGCGAGCACGGGCTCCTCGTCTTCGCTGCCGGCACCCACCCCATCGCCGACTGGGCGCGGCAATTGCCGACCAAGGGCGA
CCGCTACCGCGGCATCCTGCGCGATGTCGGCCTCGCCGGGCGGCGCAGCCTGATCTGCGGCATGCATGTCCATGTCGAGG
TCGCCGACCCCGACCGGCGCGTCGCGCTGATGGACCGGCTGCTGCCATTCCAGCCGCTGCTGTTGGCCCTCTCCGTCTCC
TCGCCGTTCTGGCAGGGCAAGCCGACGGGGCTGGCCGCCTACCGCCTCAGCGCCTTCGGCGAGCTGCCGCGCACCGGCCT
GCCCGACCTGATGGGCGACGCGGCAGCCTACGAGCGCTACGTGCGCATCATGACCAATGCCGGCTCGATCCAGGATGCGA
GCTTCCTGTGGTGGTCCCTGCGCCCCTCGATCAAGTTCCCGACGCTCGAACTGCGCATCGCCGATTCCTGCACCCGGCTC
GCCGACGCCATCGTGGTCGCGGCCCTGTTCCGCTGCCTCGTGCGGCTGATCGAGCGCCGGCCCGACATCAATGCGGGGCT
CACCGGCGTCTCGCGGGCGATCGCGGCGGAGAACCTGTGGCGGGCCCAGCGCAGCGGGATCGAGGCCGAGCTGATCGACG
AGGCGTCCGAGCAGGCCTTCCCCTTCGACGCTGCCCTCGACACGCTGCTCTCGCTGATCGCGGAGGATGCCGAGGCGTTG
GGCTGCACCGAAGAGGTGGCCGATGCCCGCCGCATACTGCGCGAGGGCACCAGCGCCGACCGGCAGATCGCCGCGTTCGA
GGGCACCCGCGGCGGCGACACCAGCAACCGCCAGGCCCTCGACGCGGTGGTGGACTGGCTCGCCGAGACCACGCGGGACG
GGTGA

Upstream 100 bases:

>100_bases
CGGCTTGCTCCCGGCCAAGGTCCTTTCGGTGCGCCTCTCTGGCTGGAGCGGCCTCCCCTCCGGTGAGAGCGCGTCAGCCG
GTCGTCGGAGTCAGCACGGC

Downstream 100 bases:

>100_bases
CGGCTAACCGAACAGCTCCGCGAGGCTCACGAGGCTGAGGCTCGCGATCAGGAGGCCCACCACGACCATCAGCACCCGCG
CCGGGATCACCCGCACCAGC

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSAHPYRFGIEEEYFLADAETRGTPRRSVKPFHQAAGERLPEIGRELLQCQVEVCTPPSTAFPAAHAALREQRQALAELG
REHGLLVFAAGTHPIADWARQLPTKGDRYRGILRDVGLAGRRSLICGMHVHVEVADPDRRVALMDRLLPFQPLLLALSVS
SPFWQGKPTGLAAYRLSAFGELPRTGLPDLMGDAAAYERYVRIMTNAGSIQDASFLWWSLRPSIKFPTLELRIADSCTRL
ADAIVVAALFRCLVRLIERRPDINAGLTGVSRAIAAENLWRAQRSGIEAELIDEASEQAFPFDAALDTLLSLIAEDAEAL
GCTEEVADARRILREGTSADRQIAAFEGTRGGDTSNRQALDAVVDWLAETTRDG

Sequences:

>Translated_374_residues
MSAHPYRFGIEEEYFLADAETRGTPRRSVKPFHQAAGERLPEIGRELLQCQVEVCTPPSTAFPAAHAALREQRQALAELG
REHGLLVFAAGTHPIADWARQLPTKGDRYRGILRDVGLAGRRSLICGMHVHVEVADPDRRVALMDRLLPFQPLLLALSVS
SPFWQGKPTGLAAYRLSAFGELPRTGLPDLMGDAAAYERYVRIMTNAGSIQDASFLWWSLRPSIKFPTLELRIADSCTRL
ADAIVVAALFRCLVRLIERRPDINAGLTGVSRAIAAENLWRAQRSGIEAELIDEASEQAFPFDAALDTLLSLIAEDAEAL
GCTEEVADARRILREGTSADRQIAAFEGTRGGDTSNRQALDAVVDWLAETTRDG
>Mature_373_residues
SAHPYRFGIEEEYFLADAETRGTPRRSVKPFHQAAGERLPEIGRELLQCQVEVCTPPSTAFPAAHAALREQRQALAELGR
EHGLLVFAAGTHPIADWARQLPTKGDRYRGILRDVGLAGRRSLICGMHVHVEVADPDRRVALMDRLLPFQPLLLALSVSS
PFWQGKPTGLAAYRLSAFGELPRTGLPDLMGDAAAYERYVRIMTNAGSIQDASFLWWSLRPSIKFPTLELRIADSCTRLA
DAIVVAALFRCLVRLIERRPDINAGLTGVSRAIAAENLWRAQRSGIEAELIDEASEQAFPFDAALDTLLSLIAEDAEALG
CTEEVADARRILREGTSADRQIAAFEGTRGGDTSNRQALDAVVDWLAETTRDG

Specific function: ATP-dependent carboxylate-amine ligase [H]

COG id: COG2170

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carboxylate-amine ligase family [H]

Homologues:

Organism=Escherichia coli, GI1786795, Length=303, Percent_Identity=26.4026402640264, Blast_Score=111, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011793
- InterPro:   IPR006336 [H]

Pfam domain/function: PF04107 GCS2 [H]

EC number: NA

Molecular weight: Translated: 41095; Mature: 40964

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHPYRFGIEEEYFLADAETRGTPRRSVKPFHQAAGERLPEIGRELLQCQVEVCTPPST
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AFPAAHAALREQRQALAELGREHGLLVFAAGTHPIADWARQLPTKGDRYRGILRDVGLAG
CCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHCCCCC
RRSLICGMHVHVEVADPDRRVALMDRLLPFQPLLLALSVSSPFWQGKPTGLAAYRLSAFG
CCCEEEEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ELPRTGLPDLMGDAAAYERYVRIMTNAGSIQDASFLWWSLRPSIKFPTLELRIADSCTRL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEEHHHHHHH
ADAIVVAALFRCLVRLIERRPDINAGLTGVSRAIAAENLWRAQRSGIEAELIDEASEQAF
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
PFDAALDTLLSLIAEDAEALGCTEEVADARRILREGTSADRQIAAFEGTRGGDTSNRQAL
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHH
DAVVDWLAETTRDG
HHHHHHHHHHCCCC
>Mature Secondary Structure 
SAHPYRFGIEEEYFLADAETRGTPRRSVKPFHQAAGERLPEIGRELLQCQVEVCTPPST
CCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AFPAAHAALREQRQALAELGREHGLLVFAAGTHPIADWARQLPTKGDRYRGILRDVGLAG
CCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHCCCCC
RRSLICGMHVHVEVADPDRRVALMDRLLPFQPLLLALSVSSPFWQGKPTGLAAYRLSAFG
CCCEEEEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ELPRTGLPDLMGDAAAYERYVRIMTNAGSIQDASFLWWSLRPSIKFPTLELRIADSCTRL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEEHHHHHHH
ADAIVVAALFRCLVRLIERRPDINAGLTGVSRAIAAENLWRAQRSGIEAELIDEASEQAF
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
PFDAALDTLLSLIAEDAEALGCTEEVADARRILREGTSADRQIAAFEGTRGGDTSNRQAL
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHH
DAVVDWLAETTRDG
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA