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The map label for this gene is purF [H]

Identifier: 218532188

GI number: 218532188

Start: 4540720

End: 4542195

Strand: Reverse

Name: purF [H]

Synonym: Mchl_4300

Alternate gene names: 218532188

Gene position: 4542195-4540720 (Counterclockwise)

Preceding gene: 218532189

Following gene: 218532187

Centisome position: 78.61

GC content: 68.09

Gene sequence:

>1476_bases
ATGTCTGTTGTCAGCGCGAGCGCCGATGTCCGCAACCTGGACATCGACGGGGATACGCTTCGCGAGGAATGCGGCGTCTT
CGGCATCTACGGGCACGACGACGCCTCGGCCATCACGGCGCTCGGCCTGCACGCCTTGCAGCATCGCGGCCAGGAGGCGG
CCGGCATCGTCTCCTACGACGACGGCGTGTTCCACTCGGAGCGCCGCCAGGGCCTCGTCGGGGATTCCTTCTCCGACCGC
GCCACGATCGAGCGGCTGGCCGGCCGCTCGGCCATCGGCCACGTGCGCTACTCGACCACCGGCGGCACGATTCTTCGCAA
CGTCCAGCCGCTCTTCGCCGAACTCGCCGGCGGCGGGCTGGCGGTGGCGCATAACGGCAACCTCACCAACGCGCTGTCGA
TCCGCCGCGACCTCGTGCGTGACGGCGCCATCACGCAATCGACCTCGGACACCGAGGTGATCCTCCATCTGGCCGCCCGC
TCGCGCAAACCGCGCATCGTCGAGCGCTTCATCGACGCGCTGCAGCAGATCCAGGGCGCCTACGCCATCGTCGCGCTGAC
CAACAAGAAGCTCATCGGCGCCCGCGACCCGCTCGGCATCCGCCCGCTGGTGCTCGGCGAACTCGACGGCCGCTACATCC
TTGCCTCCGAATCCTGCGCGCTCGACATCATCGGCGCCCGCTTCATCCGCGATGTCGAGAACGGCGAAGTGGTGGTGATC
TCGGAAGAGGGCGTCGAGTCGATCCGCTTTGCGGAGAAGCAGCCGATGCGCCCCTGCATCTTCGAGTACATCTACTTCGC
CCGCCCCGACTCGGTGGTGAACGGCAAGAGCGTCTACGGCGTGCGCAAGGCCATCGGCCAGGAACTCGCCCGCGAAGCGG
CGCCGGAGGCCGACGTGGTGATCCCGGTGCCGGATTCCGGCGTGCCGGCGGCGCTTGGCTTCGCGCAGGAAGCAGGTCTG
CCCTTCGAGATGGGCATCATCCGCAACCACTATGTCGGGCGCACCTTCATCCAGCCGACCCAGACCGTGCGCGAACTCGG
GGTGCGGATGAAGCACTCGGCCAACCGCGCCGCGGTCGAGGGCAAGCGCATCGTGCTCGTGGACGACAGCCTCGTGCGCG
GCACCACCTCGGTGAAGATCGTGCGGATGATGCGCGATGCCGGCGCCCGCGAGGTGCATTTCCGCATCGCCTCGCCGCCG
ATCACGTATCCTGACTTCTACGGCATCGACACGCCCGAGCGCGAGAAGCTCTTGGCCGCCACTCACGACCTCGAGGGGAT
GCGCCAATATATCGGCGCCGATTCCCTGGCCTTCCTGTCGATCCCCGGCCTCTACCGGGCGATGGGCGAGGAGGCGCGCA
ACGCCGACTGCCCGCAATACACCGACCATTGCTTCACCGGCGACTACCCGACCGGCCTGACCGATCTGGCGATCGCCGGG
CCCAAGCGCTTCGCCATGCTGGCCGAGGCGGACTGA

Upstream 100 bases:

>100_bases
TCGCGGCCGAGGTCGCGGGCGGCACGCGTCTCCGGCCGTTTCCCGCCGTGCCGGATGCGAGGCCCGCGAGACCCGCGCGC
CTCCAGGATTCGAGATCGAC

Downstream 100 bases:

>100_bases
AGTCGATGGCCGACAAAATCCTCGACGGTCGGGTGGCCGTCGTCACCGGCGCCTCCCGCGGCATCGGCCGCGCCGCCGCC
CTGGCCCTCGCGGAAGCCGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSVVSASADVRNLDIDGDTLREECGVFGIYGHDDASAITALGLHALQHRGQEAAGIVSYDDGVFHSERRQGLVGDSFSDR
ATIERLAGRSAIGHVRYSTTGGTILRNVQPLFAELAGGGLAVAHNGNLTNALSIRRDLVRDGAITQSTSDTEVILHLAAR
SRKPRIVERFIDALQQIQGAYAIVALTNKKLIGARDPLGIRPLVLGELDGRYILASESCALDIIGARFIRDVENGEVVVI
SEEGVESIRFAEKQPMRPCIFEYIYFARPDSVVNGKSVYGVRKAIGQELAREAAPEADVVIPVPDSGVPAALGFAQEAGL
PFEMGIIRNHYVGRTFIQPTQTVRELGVRMKHSANRAAVEGKRIVLVDDSLVRGTTSVKIVRMMRDAGAREVHFRIASPP
ITYPDFYGIDTPEREKLLAATHDLEGMRQYIGADSLAFLSIPGLYRAMGEEARNADCPQYTDHCFTGDYPTGLTDLAIAG
PKRFAMLAEAD

Sequences:

>Translated_491_residues
MSVVSASADVRNLDIDGDTLREECGVFGIYGHDDASAITALGLHALQHRGQEAAGIVSYDDGVFHSERRQGLVGDSFSDR
ATIERLAGRSAIGHVRYSTTGGTILRNVQPLFAELAGGGLAVAHNGNLTNALSIRRDLVRDGAITQSTSDTEVILHLAAR
SRKPRIVERFIDALQQIQGAYAIVALTNKKLIGARDPLGIRPLVLGELDGRYILASESCALDIIGARFIRDVENGEVVVI
SEEGVESIRFAEKQPMRPCIFEYIYFARPDSVVNGKSVYGVRKAIGQELAREAAPEADVVIPVPDSGVPAALGFAQEAGL
PFEMGIIRNHYVGRTFIQPTQTVRELGVRMKHSANRAAVEGKRIVLVDDSLVRGTTSVKIVRMMRDAGAREVHFRIASPP
ITYPDFYGIDTPEREKLLAATHDLEGMRQYIGADSLAFLSIPGLYRAMGEEARNADCPQYTDHCFTGDYPTGLTDLAIAG
PKRFAMLAEAD
>Mature_490_residues
SVVSASADVRNLDIDGDTLREECGVFGIYGHDDASAITALGLHALQHRGQEAAGIVSYDDGVFHSERRQGLVGDSFSDRA
TIERLAGRSAIGHVRYSTTGGTILRNVQPLFAELAGGGLAVAHNGNLTNALSIRRDLVRDGAITQSTSDTEVILHLAARS
RKPRIVERFIDALQQIQGAYAIVALTNKKLIGARDPLGIRPLVLGELDGRYILASESCALDIIGARFIRDVENGEVVVIS
EEGVESIRFAEKQPMRPCIFEYIYFARPDSVVNGKSVYGVRKAIGQELAREAAPEADVVIPVPDSGVPAALGFAQEAGLP
FEMGIIRNHYVGRTFIQPTQTVRELGVRMKHSANRAAVEGKRIVLVDDSLVRGTTSVKIVRMMRDAGAREVHFRIASPPI
TYPDFYGIDTPEREKLLAATHDLEGMRQYIGADSLAFLSIPGLYRAMGEEARNADCPQYTDHCFTGDYPTGLTDLAIAGP
KRFAMLAEAD

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=509, Percent_Identity=39.2927308447937, Blast_Score=352, Evalue=5e-97,
Organism=Escherichia coli, GI1788651, Length=475, Percent_Identity=41.2631578947368, Blast_Score=324, Evalue=9e-90,
Organism=Escherichia coli, GI1790167, Length=228, Percent_Identity=31.5789473684211, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=37.6299376299376, Blast_Score=308, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6323958, Length=481, Percent_Identity=36.7983367983368, Blast_Score=259, Evalue=6e-70,
Organism=Drosophila melanogaster, GI24659598, Length=495, Percent_Identity=41.2121212121212, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI28573187, Length=495, Percent_Identity=41.010101010101, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24659604, Length=467, Percent_Identity=41.7558886509636, Blast_Score=348, Evalue=7e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53272; Mature: 53141

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVSASADVRNLDIDGDTLREECGVFGIYGHDDASAITALGLHALQHRGQEAAGIVSYD
CCEEECCCCCEEECCCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEC
DGVFHSERRQGLVGDSFSDRATIERLAGRSAIGHVRYSTTGGTILRNVQPLFAELAGGGL
CCCCHHHHHCCCCCCCCCHHHHHHHHHCCCHHCEEEEECCCCHHHHHHHHHHHHHCCCCE
AVAHNGNLTNALSIRRDLVRDGAITQSTSDTEVILHLAARSRKPRIVERFIDALQQIQGA
EEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHEEHHCCCCCHHHHHHHHHHHHHCCC
YAIVALTNKKLIGARDPLGIRPLVLGELDGRYILASESCALDIIGARFIRDVENGEVVVI
EEEEEEECCCEECCCCCCCCCCEEEECCCCCEEEECCCCEEHHHHHHHHHHCCCCCEEEE
SEEGVESIRFAEKQPMRPCIFEYIYFARPDSVVNGKSVYGVRKAIGQELAREAAPEADVV
ECCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
IPVPDSGVPAALGFAQEAGLPFEMGIIRNHYVGRTFIQPTQTVRELGVRMKHSANRAAVE
EECCCCCCCHHHHHHHHCCCCEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEC
GKRIVLVDDSLVRGTTSVKIVRMMRDAGAREVHFRIASPPITYPDFYGIDTPEREKLLAA
CCEEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
THDLEGMRQYIGADSLAFLSIPGLYRAMGEEARNADCPQYTDHCFTGDYPTGLTDLAIAG
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHEECC
PKRFAMLAEAD
CCCEEEEECCC
>Mature Secondary Structure 
SVVSASADVRNLDIDGDTLREECGVFGIYGHDDASAITALGLHALQHRGQEAAGIVSYD
CEEECCCCCEEECCCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEC
DGVFHSERRQGLVGDSFSDRATIERLAGRSAIGHVRYSTTGGTILRNVQPLFAELAGGGL
CCCCHHHHHCCCCCCCCCHHHHHHHHHCCCHHCEEEEECCCCHHHHHHHHHHHHHCCCCE
AVAHNGNLTNALSIRRDLVRDGAITQSTSDTEVILHLAARSRKPRIVERFIDALQQIQGA
EEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHEEHHCCCCCHHHHHHHHHHHHHCCC
YAIVALTNKKLIGARDPLGIRPLVLGELDGRYILASESCALDIIGARFIRDVENGEVVVI
EEEEEEECCCEECCCCCCCCCCEEEECCCCCEEEECCCCEEHHHHHHHHHHCCCCCEEEE
SEEGVESIRFAEKQPMRPCIFEYIYFARPDSVVNGKSVYGVRKAIGQELAREAAPEADVV
ECCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
IPVPDSGVPAALGFAQEAGLPFEMGIIRNHYVGRTFIQPTQTVRELGVRMKHSANRAAVE
EECCCCCCCHHHHHHHHCCCCEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEC
GKRIVLVDDSLVRGTTSVKIVRMMRDAGAREVHFRIASPPITYPDFYGIDTPEREKLLAA
CCEEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
THDLEGMRQYIGADSLAFLSIPGLYRAMGEEARNADCPQYTDHCFTGDYPTGLTDLAIAG
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHEECC
PKRFAMLAEAD
CCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA