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The map label for this gene is xsc [H]

Identifier: 218532119

GI number: 218532119

Start: 4441041

End: 4442816

Strand: Reverse

Name: xsc [H]

Synonym: Mchl_4214

Alternate gene names: 218532119

Gene position: 4442816-4441041 (Counterclockwise)

Preceding gene: 218532122

Following gene: 218532114

Centisome position: 76.89

GC content: 67.12

Gene sequence:

>1776_bases
ATGAGGATGACGACCGAGGAGGCCTTCATCAAAGTCCTTCAGATGCACGGCATCGAGCACGCCTTCGGCATCATCGGCTC
GGCGATGATGCCGGTCTCCGACCTGTTTCCCAAGGCCGGCATCACCTTCTGGGACTGCGCGCACGAGAGCAACGCGGCGC
TGATCGCCGACGGCTATTCGCGGGTGACCGGCAAGATGGCGATGGCGGTGGCCCAGAACGGGCCGGGCGTGACCGGCTTC
GTCACGGCGATCAAGACCGCCTACTGGAACCACACGCCGATGCTGCTGGTGACGCCGCAGGCGGCCAACAAGACGATCGG
CCAGGGCGGCTTCCAAGAGGTCGAGCAGATGGCCCTGTTCGAGGACATGGTCTGCTATCAGGAGGAGGTGCGCGACCCGA
GCCGCGTCGCCGAGGTGCTCAACCGGGTGATCGAGAAGGCGTGGCGGGGCTGCGCACCGGCGCAGATCAACGTCCCGCGC
GATTTCTGGACGCATCAAGTCGACACTGCGCTCCCGCAGATCGTCCGGTTGGAGCGGCCGACAGGCGGGCGCCAAGCCAT
CGCCGAGGCCGCGACGCTGCTGTCGAAGGCGCAATTCCCGGTCATCCTGAACGGCGCGGGCGTCGTGATCGGCGATGCGA
TCCAGGAATCCGTCGCGCTGGCTGAGCGGCTCGATGCGCCGGTGGCCTGCGGCTACCAGCACAACGATTCCTTCCCCGGC
TCGCATCCGTTGGCCGTCGGCCCGCTCGGCTACAACGGCTCGAAGGCGGCGATGGAGCTGATCGCCAAGGCCGACGTGGT
GCTGGCGCTCGGCACTCGCCTCAACCCGTTCTCGACCCTGCCGGGCTACGGCATCGATTACTGGCCGAAAGACGCCAAGA
TCATTCAGGTCGACATCAACCCCGACCGGATCGGCCTGACAAAGCCGATCTCCGTGGGCATCTGCGGCGACGCCAAGCAC
GTCGCGCAGGAATTGCTGCAACAGCTGACGCCGGATGCCGGCAATGCCGGCCGCGAGGAGCGCCAGGCGCTCATCCACCG
CACCAAATCCGCCTGGGCCCAGGCGCTGACCTCGCTCGACCACGAGGACGACGACGAGGGCACGCGCTGGAACGTCGAGG
CGCGGGAGCGCGAAAGCGAGAAGATGTCGCCGCGCCAAGCGTGGCGAGCGATCCAGGCGGGCCTGCCCGCCGACGCGATC
CTCTCGACCGACATCGGCAACAACTGCGCCATCGGCAACGCCTACCCGACCTTCGACCAGGGCCGGCGATACCTCGCGCC
GGGCATGTTTGGCCCCTGCGGCTACGGCTTCCCGGCGATCATCGGCGCCAAGATCGGCTGCCCGGACGTGCCGGTCTTCG
GTTTCGCGGGCGACGGCGCCTTCGGCATCTCGATGAACGAGATGACCTCGATCGGCCGCGACGAGTGGCCGGCGATCACC
ATGGTGATCTTCCGCAATTTCCAGTGGGGCGCCGAGAAGCGCAACTCAATCCTCTGGTACGACAACAACTTCGTCGGCAC
CGAGCTCGACAGCAAGCTGAGCTACGCCAAGATCGCTGAAGGGTGCGGCTTCCGCGGCGTGCGTGTCGAGACCCAGCGGG
CGTTGACGGAAGCGATCCAGCAGGCGGGCGAGGCGCAAGGCGAGGGCGTCACGACCTTCATCGAGGTGATGCTCAACCAG
GAACTCGGCGAGCCCTTCCGTCGCGACGCGATGAAGAAGCCGGTCGTCGTCGCCGGCATCGACCGCGCCGACATGCGGCC
GCAGCGCGTGGCCTGA

Upstream 100 bases:

>100_bases
TAGATTTCGCCGAAGCGAGGATGCGGCGGACGTGGGTCCGCCCGAGCCGACGAGCGGCCGATCGCAACGAGCCAGCACAA
CCAGGGTGGGAGAGCGCGCC

Downstream 100 bases:

>100_bases
TAGACCGCGTCACCGAGGAGTCCGGGGTTCCAAGGGCGAGCCCTTAGCGGGGTCCGGGCGGAGCCCGGGAAAACCTGGGG
CCGATGACCTTGGCCCCTTG

Product: sulfoacetaldehyde acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MRMTTEEAFIKVLQMHGIEHAFGIIGSAMMPVSDLFPKAGITFWDCAHESNAALIADGYSRVTGKMAMAVAQNGPGVTGF
VTAIKTAYWNHTPMLLVTPQAANKTIGQGGFQEVEQMALFEDMVCYQEEVRDPSRVAEVLNRVIEKAWRGCAPAQINVPR
DFWTHQVDTALPQIVRLERPTGGRQAIAEAATLLSKAQFPVILNGAGVVIGDAIQESVALAERLDAPVACGYQHNDSFPG
SHPLAVGPLGYNGSKAAMELIAKADVVLALGTRLNPFSTLPGYGIDYWPKDAKIIQVDINPDRIGLTKPISVGICGDAKH
VAQELLQQLTPDAGNAGREERQALIHRTKSAWAQALTSLDHEDDDEGTRWNVEARERESEKMSPRQAWRAIQAGLPADAI
LSTDIGNNCAIGNAYPTFDQGRRYLAPGMFGPCGYGFPAIIGAKIGCPDVPVFGFAGDGAFGISMNEMTSIGRDEWPAIT
MVIFRNFQWGAEKRNSILWYDNNFVGTELDSKLSYAKIAEGCGFRGVRVETQRALTEAIQQAGEAQGEGVTTFIEVMLNQ
ELGEPFRRDAMKKPVVVAGIDRADMRPQRVA

Sequences:

>Translated_591_residues
MRMTTEEAFIKVLQMHGIEHAFGIIGSAMMPVSDLFPKAGITFWDCAHESNAALIADGYSRVTGKMAMAVAQNGPGVTGF
VTAIKTAYWNHTPMLLVTPQAANKTIGQGGFQEVEQMALFEDMVCYQEEVRDPSRVAEVLNRVIEKAWRGCAPAQINVPR
DFWTHQVDTALPQIVRLERPTGGRQAIAEAATLLSKAQFPVILNGAGVVIGDAIQESVALAERLDAPVACGYQHNDSFPG
SHPLAVGPLGYNGSKAAMELIAKADVVLALGTRLNPFSTLPGYGIDYWPKDAKIIQVDINPDRIGLTKPISVGICGDAKH
VAQELLQQLTPDAGNAGREERQALIHRTKSAWAQALTSLDHEDDDEGTRWNVEARERESEKMSPRQAWRAIQAGLPADAI
LSTDIGNNCAIGNAYPTFDQGRRYLAPGMFGPCGYGFPAIIGAKIGCPDVPVFGFAGDGAFGISMNEMTSIGRDEWPAIT
MVIFRNFQWGAEKRNSILWYDNNFVGTELDSKLSYAKIAEGCGFRGVRVETQRALTEAIQQAGEAQGEGVTTFIEVMLNQ
ELGEPFRRDAMKKPVVVAGIDRADMRPQRVA
>Mature_591_residues
MRMTTEEAFIKVLQMHGIEHAFGIIGSAMMPVSDLFPKAGITFWDCAHESNAALIADGYSRVTGKMAMAVAQNGPGVTGF
VTAIKTAYWNHTPMLLVTPQAANKTIGQGGFQEVEQMALFEDMVCYQEEVRDPSRVAEVLNRVIEKAWRGCAPAQINVPR
DFWTHQVDTALPQIVRLERPTGGRQAIAEAATLLSKAQFPVILNGAGVVIGDAIQESVALAERLDAPVACGYQHNDSFPG
SHPLAVGPLGYNGSKAAMELIAKADVVLALGTRLNPFSTLPGYGIDYWPKDAKIIQVDINPDRIGLTKPISVGICGDAKH
VAQELLQQLTPDAGNAGREERQALIHRTKSAWAQALTSLDHEDDDEGTRWNVEARERESEKMSPRQAWRAIQAGLPADAI
LSTDIGNNCAIGNAYPTFDQGRRYLAPGMFGPCGYGFPAIIGAKIGCPDVPVFGFAGDGAFGISMNEMTSIGRDEWPAIT
MVIFRNFQWGAEKRNSILWYDNNFVGTELDSKLSYAKIAEGCGFRGVRVETQRALTEAIQQAGEAQGEGVTTFIEVMLNQ
ELGEPFRRDAMKKPVVVAGIDRADMRPQRVA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=607, Percent_Identity=23.3937397034596, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI93004078, Length=489, Percent_Identity=24.7443762781186, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=24.8239436619718, Blast_Score=202, Evalue=7e-53,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=27.4864376130199, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1787096, Length=578, Percent_Identity=25.9515570934256, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1786717, Length=575, Percent_Identity=24, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1788716, Length=498, Percent_Identity=26.1044176706827, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17542570, Length=580, Percent_Identity=24.8275862068966, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=493, Percent_Identity=24.5436105476673, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531301, Length=493, Percent_Identity=24.1379310344828, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323755, Length=580, Percent_Identity=23.2758620689655, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320816, Length=579, Percent_Identity=23.4887737478411, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6320123, Length=504, Percent_Identity=22.4206349206349, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922626, Length=500, Percent_Identity=25, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017820
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.3.3.15 [H]

Molecular weight: Translated: 64221; Mature: 64221

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMTTEEAFIKVLQMHGIEHAFGIIGSAMMPVSDLFPKAGITFWDCAHESNAALIADGYS
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHCCCCCCEEEECCCCCCCEEEECCHH
RVTGKMAMAVAQNGPGVTGFVTAIKTAYWNHTPMLLVTPQAANKTIGQGGFQEVEQMALF
HHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
EDMVCYQEEVRDPSRVAEVLNRVIEKAWRGCAPAQINVPRDFWTHQVDTALPQIVRLERP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHEECCC
TGGRQAIAEAATLLSKAQFPVILNGAGVVIGDAIQESVALAERLDAPVACGYQHNDSFPG
CCHHHHHHHHHHHHHHCCCCEEECCCCEEEHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
SHPLAVGPLGYNGSKAAMELIAKADVVLALGTRLNPFSTLPGYGIDYWPKDAKIIQVDIN
CCCEEECCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECC
PDRIGLTKPISVGICGDAKHVAQELLQQLTPDAGNAGREERQALIHRTKSAWAQALTSLD
CCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
HEDDDEGTRWNVEARERESEKMSPRQAWRAIQAGLPADAILSTDIGNNCAIGNAYPTFDQ
CCCCCCCCEECCHHHHHHHHCCCHHHHHHHHHCCCCHHHHEECCCCCCCCCCCCCCCHHH
GRRYLAPGMFGPCGYGFPAIIGAKIGCPDVPVFGFAGDGAFGISMNEMTSIGRDEWPAIT
CCEEECCCCCCCCCCCCHHHHCCCCCCCCCCEEEECCCCEEECCHHHHHHCCCCCCCEEE
MVIFRNFQWGAEKRNSILWYDNNFVGTELDSKLSYAKIAEGCGFRGVRVETQRALTEAIQ
EEEHHCCCCCCCCCCCEEEECCCEECCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
QAGEAQGEGVTTFIEVMLNQELGEPFRRDAMKKPVVVAGIDRADMRPQRVA
HHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCHHCCCCCCCC
>Mature Secondary Structure
MRMTTEEAFIKVLQMHGIEHAFGIIGSAMMPVSDLFPKAGITFWDCAHESNAALIADGYS
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHCCCCCCEEEECCCCCCCEEEECCHH
RVTGKMAMAVAQNGPGVTGFVTAIKTAYWNHTPMLLVTPQAANKTIGQGGFQEVEQMALF
HHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
EDMVCYQEEVRDPSRVAEVLNRVIEKAWRGCAPAQINVPRDFWTHQVDTALPQIVRLERP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHEECCC
TGGRQAIAEAATLLSKAQFPVILNGAGVVIGDAIQESVALAERLDAPVACGYQHNDSFPG
CCHHHHHHHHHHHHHHCCCCEEECCCCEEEHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
SHPLAVGPLGYNGSKAAMELIAKADVVLALGTRLNPFSTLPGYGIDYWPKDAKIIQVDIN
CCCEEECCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECC
PDRIGLTKPISVGICGDAKHVAQELLQQLTPDAGNAGREERQALIHRTKSAWAQALTSLD
CCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
HEDDDEGTRWNVEARERESEKMSPRQAWRAIQAGLPADAILSTDIGNNCAIGNAYPTFDQ
CCCCCCCCEECCHHHHHHHHCCCHHHHHHHHHCCCCHHHHEECCCCCCCCCCCCCCCHHH
GRRYLAPGMFGPCGYGFPAIIGAKIGCPDVPVFGFAGDGAFGISMNEMTSIGRDEWPAIT
CCEEECCCCCCCCCCCCHHHHCCCCCCCCCCEEEECCCCEEECCHHHHHHCCCCCCCEEE
MVIFRNFQWGAEKRNSILWYDNNFVGTELDSKLSYAKIAEGCGFRGVRVETQRALTEAIQ
EEEHHCCCCCCCCCCCEEEECCCEECCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
QAGEAQGEGVTTFIEVMLNQELGEPFRRDAMKKPVVVAGIDRADMRPQRVA
HHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]