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The map label for this gene is dppA [H]

Identifier: 218531963

GI number: 218531963

Start: 4267947

End: 4269518

Strand: Reverse

Name: dppA [H]

Synonym: Mchl_4051

Alternate gene names: 218531963

Gene position: 4269518-4267947 (Counterclockwise)

Preceding gene: 218531964

Following gene: 218531962

Centisome position: 73.89

GC content: 65.97

Gene sequence:

>1572_bases
ATGGCCGGTACAATGGACCGCAGACGGTTTCTTCAGGCGAGCGCCGCCGCTGTGGGTTTTGCCCAGATCAATCCCGACTT
CCTGGTCTCCTCGGCCTTCGCCCAATCGGGCAAGCCGCTGGTCTTCCTCTCGGCCGAGAACATCACCGGCAACTGGGACC
CCACCGCCCACACCACGCTCTCGCAGAAGAACATCGAGGGCTTCGTGATGGGCTTCCTGACCCGCACGCCGATGACCCTC
GATGACCCCGGCAAGGTCGTCTACGAACTCGCTACCGACATCCGGTTGCTCGATCCGTACCGCCTGCAGATCAAGCTGCG
CAAGGACGTGCAGTTTCACGACGGCAAGCCGTTCGGGCCCGAGGACGTCAAGGCGACCTTCGAGTACGGCGCGGGCAAGG
ACCGGCCGGCGCAGTGGTATCCCGGCCCGACCGAGACGCTGACGATCACCACGCCCGACGACGAGACCGTGATCGTCGAC
ACCTCGAAGGGCGGCTACCCCGCCCACCTCTTCATCTTCCTCGCCTCGTTCCTCCCGATCCTCTCGGCCAAGGACGTGGC
CGAGGGCCCGGGCGGCGCCCTCACCCGGCGCCTGAACGGCACCGGCCCGTTCCGCTTCGTCGAGCAGCGCGGCAACGACA
CCGTGCTCAAGGCCCATGACGGCTATTTCCGCGGCAAACCCGGCATTCCCGGCATCAACTTCACCTTCACCGGCGACTCG
ACCACGCGGATGCTGTCGCTGATGAACGGCCAAGCCTCGATCGTCGAGCGGCTCGAACCCGAGCAGGTCGAGACAGTCAA
GAACAATCCGAAGATCGCGATCAACGAGGTCGTCTCGGTCGAGAACAAGTATCTCTGGTTCCGCTGCTCCAAGCCGCCCT
TCAACGACGTGCGGGTGCGCATGGCCGCCTGCCACTCGATCGACCGGGCGATGCTCTTGGAGATCCTCGGCGCGGCGGGC
CACGCCTCGGCCAACTTCATCTCGCCGGTGAAGTTCGGCTACGTCGACCTGAAGAACTACCCGGCCTACGACCCGGCCAA
GGCCCAGGCGCTGCTGGCCGAGGCGGGCTTCCCCAAGGGCAAGGGGCTGCCGCCGCTCGAATACATCACCTCGGTCGGGT
TCTACCCGAAGACCAAGGAGTACGGCGAGGTCATCACCGCGATGCTCAACGAGCAGGGCTTTCCGGTGAGCCTCACGGTG
CTGGAGCCGGCGGCCTGGAACGAGCGGCTCTACCATCGCCCCGGCGGCGGACCCGGCCACATGGTCGATTGCGGCTGGTC
CACCGCCTCGCCCGAGCCGGATCTGGTGCTGCGCACCCACTTCCACTCCTCCTCGCACCGCATCACCGGCATCGAGGATG
CGGAGATTGACGCGAGCCTCGACAAGGAGCGCGCGGCGCCGACGCTGGAGGAGCGCAAGGCCATCCTCCAGAACGAGACG
ATGCCGCTGCTGGCCGCCAAGATGCCGGCGCTGTCGCTGTTCACCTCGGTGATGATCCACGCGATGCAGCAGGAGCTGAA
GGGCCTCTACATCTATCCGGACGGGTCGATCGACGCCTCCAAAACCGCCTGA

Upstream 100 bases:

>100_bases
CCAAGCTGACCGGTCTGTGTGATTTGTGGCTTCTGGCACGGGTCCTGCAATGTGGTTTTTGACCACTTTGATGGATGCGT
GGGAACTCGGGAGCCTCAGC

Downstream 100 bases:

>100_bases
CTGAACTGTCCGCGCCCGGCGCGTGACCCGGCCCGCCTCCGCGGGCGGCCGGGCCCCGCCCCGCCGATTCCGTCACCGGA
CCCGATGCCCCGATGTTCGT

Product: extracellular solute-binding protein family 5

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MAGTMDRRRFLQASAAAVGFAQINPDFLVSSAFAQSGKPLVFLSAENITGNWDPTAHTTLSQKNIEGFVMGFLTRTPMTL
DDPGKVVYELATDIRLLDPYRLQIKLRKDVQFHDGKPFGPEDVKATFEYGAGKDRPAQWYPGPTETLTITTPDDETVIVD
TSKGGYPAHLFIFLASFLPILSAKDVAEGPGGALTRRLNGTGPFRFVEQRGNDTVLKAHDGYFRGKPGIPGINFTFTGDS
TTRMLSLMNGQASIVERLEPEQVETVKNNPKIAINEVVSVENKYLWFRCSKPPFNDVRVRMAACHSIDRAMLLEILGAAG
HASANFISPVKFGYVDLKNYPAYDPAKAQALLAEAGFPKGKGLPPLEYITSVGFYPKTKEYGEVITAMLNEQGFPVSLTV
LEPAAWNERLYHRPGGGPGHMVDCGWSTASPEPDLVLRTHFHSSSHRITGIEDAEIDASLDKERAAPTLEERKAILQNET
MPLLAAKMPALSLFTSVMIHAMQQELKGLYIYPDGSIDASKTA

Sequences:

>Translated_523_residues
MAGTMDRRRFLQASAAAVGFAQINPDFLVSSAFAQSGKPLVFLSAENITGNWDPTAHTTLSQKNIEGFVMGFLTRTPMTL
DDPGKVVYELATDIRLLDPYRLQIKLRKDVQFHDGKPFGPEDVKATFEYGAGKDRPAQWYPGPTETLTITTPDDETVIVD
TSKGGYPAHLFIFLASFLPILSAKDVAEGPGGALTRRLNGTGPFRFVEQRGNDTVLKAHDGYFRGKPGIPGINFTFTGDS
TTRMLSLMNGQASIVERLEPEQVETVKNNPKIAINEVVSVENKYLWFRCSKPPFNDVRVRMAACHSIDRAMLLEILGAAG
HASANFISPVKFGYVDLKNYPAYDPAKAQALLAEAGFPKGKGLPPLEYITSVGFYPKTKEYGEVITAMLNEQGFPVSLTV
LEPAAWNERLYHRPGGGPGHMVDCGWSTASPEPDLVLRTHFHSSSHRITGIEDAEIDASLDKERAAPTLEERKAILQNET
MPLLAAKMPALSLFTSVMIHAMQQELKGLYIYPDGSIDASKTA
>Mature_522_residues
AGTMDRRRFLQASAAAVGFAQINPDFLVSSAFAQSGKPLVFLSAENITGNWDPTAHTTLSQKNIEGFVMGFLTRTPMTLD
DPGKVVYELATDIRLLDPYRLQIKLRKDVQFHDGKPFGPEDVKATFEYGAGKDRPAQWYPGPTETLTITTPDDETVIVDT
SKGGYPAHLFIFLASFLPILSAKDVAEGPGGALTRRLNGTGPFRFVEQRGNDTVLKAHDGYFRGKPGIPGINFTFTGDST
TRMLSLMNGQASIVERLEPEQVETVKNNPKIAINEVVSVENKYLWFRCSKPPFNDVRVRMAACHSIDRAMLLEILGAAGH
ASANFISPVKFGYVDLKNYPAYDPAKAQALLAEAGFPKGKGLPPLEYITSVGFYPKTKEYGEVITAMLNEQGFPVSLTVL
EPAAWNERLYHRPGGGPGHMVDCGWSTASPEPDLVLRTHFHSSSHRITGIEDAEIDASLDKERAAPTLEERKAILQNETM
PLLAAKMPALSLFTSVMIHAMQQELKGLYIYPDGSIDASKTA

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=436, Percent_Identity=26.1467889908257, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1787551, Length=461, Percent_Identity=24.0780911062907, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787052, Length=501, Percent_Identity=24.750499001996, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787762, Length=429, Percent_Identity=26.1072261072261, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1789887, Length=336, Percent_Identity=24.4047619047619, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57330; Mature: 57199

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTMDRRRFLQASAAAVGFAQINPDFLVSSAFAQSGKPLVFLSAENITGNWDPTAHTTL
CCCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEE
SQKNIEGFVMGFLTRTPMTLDDPGKVVYELATDIRLLDPYRLQIKLRKDVQFHDGKPFGP
CCCCCCHHEEHHHHCCCCCCCCCCHHHHHHHCCEEEECCEEEEEEEECCCEECCCCCCCH
EDVKATFEYGAGKDRPAQWYPGPTETLTITTPDDETVIVDTSKGGYPAHLFIFLASFLPI
HHHHHHEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHH
LSAKDVAEGPGGALTRRLNGTGPFRFVEQRGNDTVLKAHDGYFRGKPGIPGINFTFTGDS
HCCCCCCCCCCCCEEEECCCCCCEEEHHHCCCCCEEEECCCEECCCCCCCCCEEEECCCC
TTRMLSLMNGQASIVERLEPEQVETVKNNPKIAINEVVSVENKYLWFRCSKPPFNDVRVR
HHHHHHHHCCHHHHHHHCCCHHHHHHCCCCCEEEEHEEECCCEEEEEEECCCCCHHHHHH
MAACHSIDRAMLLEILGAAGHASANFISPVKFGYVDLKNYPAYDPAKAQALLAEAGFPKG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECEEEECCCCCCCCHHHHHHHHHCCCCCC
KGLPPLEYITSVGFYPKTKEYGEVITAMLNEQGFPVSLTVLEPAAWNERLYHRPGGGPGH
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEECCCCCCCC
MVDCGWSTASPEPDLVLRTHFHSSSHRITGIEDAEIDASLDKERAAPTLEERKAILQNET
EEECCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHCCC
MPLLAAKMPALSLFTSVMIHAMQQELKGLYIYPDGSIDASKTA
CCHHHHHCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
AGTMDRRRFLQASAAAVGFAQINPDFLVSSAFAQSGKPLVFLSAENITGNWDPTAHTTL
CCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEE
SQKNIEGFVMGFLTRTPMTLDDPGKVVYELATDIRLLDPYRLQIKLRKDVQFHDGKPFGP
CCCCCCHHEEHHHHCCCCCCCCCCHHHHHHHCCEEEECCEEEEEEEECCCEECCCCCCCH
EDVKATFEYGAGKDRPAQWYPGPTETLTITTPDDETVIVDTSKGGYPAHLFIFLASFLPI
HHHHHHEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHH
LSAKDVAEGPGGALTRRLNGTGPFRFVEQRGNDTVLKAHDGYFRGKPGIPGINFTFTGDS
HCCCCCCCCCCCCEEEECCCCCCEEEHHHCCCCCEEEECCCEECCCCCCCCCEEEECCCC
TTRMLSLMNGQASIVERLEPEQVETVKNNPKIAINEVVSVENKYLWFRCSKPPFNDVRVR
HHHHHHHHCCHHHHHHHCCCHHHHHHCCCCCEEEEHEEECCCEEEEEEECCCCCHHHHHH
MAACHSIDRAMLLEILGAAGHASANFISPVKFGYVDLKNYPAYDPAKAQALLAEAGFPKG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECEEEECCCCCCCCHHHHHHHHHCCCCCC
KGLPPLEYITSVGFYPKTKEYGEVITAMLNEQGFPVSLTVLEPAAWNERLYHRPGGGPGH
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEECCCCCCCC
MVDCGWSTASPEPDLVLRTHFHSSSHRITGIEDAEIDASLDKERAAPTLEERKAILQNET
EEECCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHCCC
MPLLAAKMPALSLFTSVMIHAMQQELKGLYIYPDGSIDASKTA
CCHHHHHCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]