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The map label for this gene is ydgK [H]

Identifier: 218531660

GI number: 218531660

Start: 3935349

End: 3936734

Strand: Reverse

Name: ydgK [H]

Synonym: Mchl_3729

Alternate gene names: 218531660

Gene position: 3936734-3935349 (Counterclockwise)

Preceding gene: 218531661

Following gene: 218531658

Centisome position: 68.13

GC content: 70.2

Gene sequence:

>1386_bases
ATGCTGCCCCTGAGAGCATCCGCGACCGTGATATCCTCCCCTCGGCCCACCCTCGGAAGCCCGGCCTCCGAGGCCTCGCC
GGGCGGTTCGCCGGGAGCGGCCCAGAGCGTGCCCGCGGGCCCGCCGGCGAGCCCGTCCCCTTCGGGGCCATCCCCCTCGA
GTCCATCCCCTTGGGTCGGGCCGGGCTTTGCCGAGTTCGTCGGCATCGTCGCGCTGATGATGGCGGTCACCGCCATGTCG
ATCGACAACCTGCTGCCGGCCTTCCCGCTGATCCAGGAGCGGTTCGGGGTTGCCGACCCCAACAGTCTGCAGCTCCTCGT
CTACGTCTACATGATCGGCTTCGGGGTGGCGCAGATCGTCTACGGGCCGGTCTCGGACGCCCTCGGGCGGCGGCCGACGC
TGCTGATCAGCCTCGGCATCTACACCGCGGGCTGCATCCTCTCGATGCTGGCGCCGAGCTACGAGTGGCTGCTCGCCTCC
CGCGTCATCCAGGGCATGGGCGCGGCGGGCGGCCGGGTGCTCGCCACCGCGATCGTGCGCGACCGCTTCTCGGGGCGCGA
GATGGCGAGCGTGATGTCGCTCATCATGATGGTGTTCCTGATCGTGCCGATGCTCGGGCCGGCGATCGGCGCGACCATGC
TGCTGCTCGGCTCGTGGATCTGGGTGTTCGTCTCGATGCTGGCGCTGGGCGGCATCCTCACCCTGTGGTTCGCCCTGCGC
ATGCCCGAAACGCTGCATCCCGAGTACCGCCGCCCGCTCTCGCTGCGGGCCACGGGGCAGGCGCTCGCCACGGTGCTGCG
CAACCGCGCGGCGATCGGCTACGCCACGGCGCTCGGCCTGCTGACGGGCTGCATCATGGGCTATGTCGGCTCGGCCCAGC
AGGTGTTCGACACCGGGCTCTATCATCTCGGCGGCCTGTTCCCCGTCGCCTTCGGCGTGGTGGCCGGTGCCATGGGCGCG
GCGACGCTGATCAATTCCCGCGTCGTCCGCTGGCTCGGGATGCGGACCCTGTCGCATGCGGGCGTCACCGCCTTCACGGC
GGTCGGCCTCGTGCAGGTCGGCATTGGCCTGCACTATCAGGGGCATCCGCCGCTGGTGCTGTTCCTCTCGGTGCTGGCGC
TCAACCAGTTCCTGATTAGCTTCGCCCTGCCGAACTTCAACGCGCTGGCGCTGCAGCCGCTTGGGGCGATCGCCGGCACG
GCCTCGTCGTTCCTCGGCTTCTACACCACGATCCTGGGCGCCTTCTGCGGCTTCGTCATCGGGCATGCCTTCGACGGCAC
GGTGATGCCGGTGGCCTATGGCTACGCCCTGCTCGGTGCGCTGGCCCTGCTCACCGTGGCCTGGACCGAGCGCGGGCGCC
TTTATCGGGCCGGCGTCTCCGGCTGA

Upstream 100 bases:

>100_bases
GCAGACAACCCTCCTCCCCGTACGGGGGAGGGATAGCGCCGGCTTCCCCCGCGTTACGACACGCCAGCCCCTTGTCCCGC
CGCGCCCGGTCCCAAGCCGG

Downstream 100 bases:

>100_bases
GATCGGCGGCCCGGAAAGCGTCCGGGCCGCCGGGGTTTGTCAGGCGTTGGCGCCGCCATCGACCGTGAGGGTGGTCCCCG
TGATCTGCCGCGCCGCCGGG

Product: major facilitator superfamily MFS_1

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLPLRASATVISSPRPTLGSPASEASPGGSPGAAQSVPAGPPASPSPSGPSPSSPSPWVGPGFAEFVGIVALMMAVTAMS
IDNLLPAFPLIQERFGVADPNSLQLLVYVYMIGFGVAQIVYGPVSDALGRRPTLLISLGIYTAGCILSMLAPSYEWLLAS
RVIQGMGAAGGRVLATAIVRDRFSGREMASVMSLIMMVFLIVPMLGPAIGATMLLLGSWIWVFVSMLALGGILTLWFALR
MPETLHPEYRRPLSLRATGQALATVLRNRAAIGYATALGLLTGCIMGYVGSAQQVFDTGLYHLGGLFPVAFGVVAGAMGA
ATLINSRVVRWLGMRTLSHAGVTAFTAVGLVQVGIGLHYQGHPPLVLFLSVLALNQFLISFALPNFNALALQPLGAIAGT
ASSFLGFYTTILGAFCGFVIGHAFDGTVMPVAYGYALLGALALLTVAWTERGRLYRAGVSG

Sequences:

>Translated_461_residues
MLPLRASATVISSPRPTLGSPASEASPGGSPGAAQSVPAGPPASPSPSGPSPSSPSPWVGPGFAEFVGIVALMMAVTAMS
IDNLLPAFPLIQERFGVADPNSLQLLVYVYMIGFGVAQIVYGPVSDALGRRPTLLISLGIYTAGCILSMLAPSYEWLLAS
RVIQGMGAAGGRVLATAIVRDRFSGREMASVMSLIMMVFLIVPMLGPAIGATMLLLGSWIWVFVSMLALGGILTLWFALR
MPETLHPEYRRPLSLRATGQALATVLRNRAAIGYATALGLLTGCIMGYVGSAQQVFDTGLYHLGGLFPVAFGVVAGAMGA
ATLINSRVVRWLGMRTLSHAGVTAFTAVGLVQVGIGLHYQGHPPLVLFLSVLALNQFLISFALPNFNALALQPLGAIAGT
ASSFLGFYTTILGAFCGFVIGHAFDGTVMPVAYGYALLGALALLTVAWTERGRLYRAGVSG
>Mature_461_residues
MLPLRASATVISSPRPTLGSPASEASPGGSPGAAQSVPAGPPASPSPSGPSPSSPSPWVGPGFAEFVGIVALMMAVTAMS
IDNLLPAFPLIQERFGVADPNSLQLLVYVYMIGFGVAQIVYGPVSDALGRRPTLLISLGIYTAGCILSMLAPSYEWLLAS
RVIQGMGAAGGRVLATAIVRDRFSGREMASVMSLIMMVFLIVPMLGPAIGATMLLLGSWIWVFVSMLALGGILTLWFALR
MPETLHPEYRRPLSLRATGQALATVLRNRAAIGYATALGLLTGCIMGYVGSAQQVFDTGLYHLGGLFPVAFGVVAGAMGA
ATLINSRVVRWLGMRTLSHAGVTAFTAVGLVQVGIGLHYQGHPPLVLFLSVLALNQFLISFALPNFNALALQPLGAIAGT
ASSFLGFYTTILGAFCGFVIGHAFDGTVMPVAYGYALLGALALLTVAWTERGRLYRAGVSG

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=392, Percent_Identity=26.0204081632653, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI48994889, Length=160, Percent_Identity=31.25, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787065, Length=226, Percent_Identity=28.7610619469027, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790146, Length=167, Percent_Identity=30.5389221556886, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082312, Length=160, Percent_Identity=30.625, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790794, Length=191, Percent_Identity=28.2722513089005, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47944; Mature: 47944

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLRASATVISSPRPTLGSPASEASPGGSPGAAQSVPAGPPASPSPSGPSPSSPSPWVG
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PGFAEFVGIVALMMAVTAMSIDNLLPAFPLIQERFGVADPNSLQLLVYVYMIGFGVAQIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
YGPVSDALGRRPTLLISLGIYTAGCILSMLAPSYEWLLASRVIQGMGAAGGRVLATAIVR
HCCHHHHHCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
DRFSGREMASVMSLIMMVFLIVPMLGPAIGATMLLLGSWIWVFVSMLALGGILTLWFALR
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MPETLHPEYRRPLSLRATGQALATVLRNRAAIGYATALGLLTGCIMGYVGSAQQVFDTGL
CCHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YHLGGLFPVAFGVVAGAMGAATLINSRVVRWLGMRTLSHAGVTAFTAVGLVQVGIGLHYQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEC
GHPPLVLFLSVLALNQFLISFALPNFNALALQPLGAIAGTASSFLGFYTTILGAFCGFVI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHAFDGTVMPVAYGYALLGALALLTVAWTERGRLYRAGVSG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
>Mature Secondary Structure
MLPLRASATVISSPRPTLGSPASEASPGGSPGAAQSVPAGPPASPSPSGPSPSSPSPWVG
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PGFAEFVGIVALMMAVTAMSIDNLLPAFPLIQERFGVADPNSLQLLVYVYMIGFGVAQIV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
YGPVSDALGRRPTLLISLGIYTAGCILSMLAPSYEWLLASRVIQGMGAAGGRVLATAIVR
HCCHHHHHCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
DRFSGREMASVMSLIMMVFLIVPMLGPAIGATMLLLGSWIWVFVSMLALGGILTLWFALR
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MPETLHPEYRRPLSLRATGQALATVLRNRAAIGYATALGLLTGCIMGYVGSAQQVFDTGL
CCHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YHLGGLFPVAFGVVAGAMGAATLINSRVVRWLGMRTLSHAGVTAFTAVGLVQVGIGLHYQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEC
GHPPLVLFLSVLALNQFLISFALPNFNALALQPLGAIAGTASSFLGFYTTILGAFCGFVI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHAFDGTVMPVAYGYALLGALALLTVAWTERGRLYRAGVSG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]