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The map label for this gene is mepA [H]

Identifier: 218531539

GI number: 218531539

Start: 3800350

End: 3801279

Strand: Reverse

Name: mepA [H]

Synonym: Mchl_3607

Alternate gene names: 218531539

Gene position: 3801279-3800350 (Counterclockwise)

Preceding gene: 218531541

Following gene: 218531536

Centisome position: 65.79

GC content: 73.12

Gene sequence:

>930_bases
ATGCCGCTGACCCGCCTGACACTCCCTGCCCTCGCGCTTGCCCTTCCGGCGCTGCTGAGCCCGGCTGCGGCGCAGGACCG
CGGCAGCGTCAACCCGAAGCCGCTGCCGCCGCTCGCCAACCCGAACGATCCCTCGACGCCGGCCAAGGCCCTGTTCGGCC
GCGTCACCGGGCCGGCCAGCGGGCCGGCGCGGCCGTTCGGCTCCTACGCCAAGGGCTGCTTCTCCGGCGGCGAGGCGCTG
CCCCTCGACGGCGCGACCTGGCAGGTGATGCGGCCCTCCCGCAATCGGATGTGGGGCACGCCCCATCTCGTTGATTTCAT
CGAGCGGCTCGCCGCCCGCGCGCCGCAGGCCGGCTGGCCCGGCCTTCTCGTCGGCGACATGTCGCAGCCCCGCGGCGGGC
CGATGCTCACCGGCCACGCCTCGCATCAGCTCGGCCTCGACGTGGACGTCTGGCTCACGCCGATGCCGGACCACCGCATG
ACCCGGGCCGAGCGCGAGGAGACCTCGGCCACCGACATGGTGCGCTCGGACCGGCTCGACATCGATCCCGACGTCTGGAC
GCGCCAGCATACCGCGCTGATCCGCATGACGGCCAAGCAGCCCGAGGTGGCGCGGATCTTCGTGAACGCGGCGATCAAGA
AGGCCCTATGCCGCGAGGCGGGCGGGGATCGCAGCTGGCTCACCAAGGTGCGCCCGATGTACGGGCACAACTACCACTAC
CATATCCGCCTCGCCTGCCCGGCCGGCGACGGTGCCTGCGACGACCAGGCGCCCCCGCCCTCCGGCGACGGCTGCGGCTC
CGAACTCGATTACTGGTTCAGCGCGGCGGTGCTGAACCCGCCCAAGCCCAAGACCCCGCCGAAGCCCCGCCCTCCGATGA
CACTCGCCGGCCTGCCCGACGCCTGCCGTGCGGTGCTGCACGCGCGGTAG

Upstream 100 bases:

>100_bases
GCTTGCCATAAAAAACGCCCATGCGGCCGGGCACGTTCCGGCCGGTGCGGCACCCACCCTTGCCCCATCCTGCCCCGCCA
TCCCGGACGAGCCACCGCGC

Downstream 100 bases:

>100_bases
AGGTCGCCTACTTCTCGTGGTGTCCGGCCGAGAGCCGGTCGGTCAGCGCCATCAGCAGGCCGGACACCACGAACACCATG
TGGATGCCGACCAGCCAGGA

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MPLTRLTLPALALALPALLSPAAAQDRGSVNPKPLPPLANPNDPSTPAKALFGRVTGPASGPARPFGSYAKGCFSGGEAL
PLDGATWQVMRPSRNRMWGTPHLVDFIERLAARAPQAGWPGLLVGDMSQPRGGPMLTGHASHQLGLDVDVWLTPMPDHRM
TRAEREETSATDMVRSDRLDIDPDVWTRQHTALIRMTAKQPEVARIFVNAAIKKALCREAGGDRSWLTKVRPMYGHNYHY
HIRLACPAGDGACDDQAPPPSGDGCGSELDYWFSAAVLNPPKPKTPPKPRPPMTLAGLPDACRAVLHAR

Sequences:

>Translated_309_residues
MPLTRLTLPALALALPALLSPAAAQDRGSVNPKPLPPLANPNDPSTPAKALFGRVTGPASGPARPFGSYAKGCFSGGEAL
PLDGATWQVMRPSRNRMWGTPHLVDFIERLAARAPQAGWPGLLVGDMSQPRGGPMLTGHASHQLGLDVDVWLTPMPDHRM
TRAEREETSATDMVRSDRLDIDPDVWTRQHTALIRMTAKQPEVARIFVNAAIKKALCREAGGDRSWLTKVRPMYGHNYHY
HIRLACPAGDGACDDQAPPPSGDGCGSELDYWFSAAVLNPPKPKTPPKPRPPMTLAGLPDACRAVLHAR
>Mature_308_residues
PLTRLTLPALALALPALLSPAAAQDRGSVNPKPLPPLANPNDPSTPAKALFGRVTGPASGPARPFGSYAKGCFSGGEALP
LDGATWQVMRPSRNRMWGTPHLVDFIERLAARAPQAGWPGLLVGDMSQPRGGPMLTGHASHQLGLDVDVWLTPMPDHRMT
RAEREETSATDMVRSDRLDIDPDVWTRQHTALIRMTAKQPEVARIFVNAAIKKALCREAGGDRSWLTKVRPMYGHNYHYH
IRLACPAGDGACDDQAPPPSGDGCGSELDYWFSAAVLNPPKPKTPPKPRPPMTLAGLPDACRAVLHAR

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=255, Percent_Identity=44.7058823529412, Blast_Score=214, Evalue=8e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 33305; Mature: 33173

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTRLTLPALALALPALLSPAAAQDRGSVNPKPLPPLANPNDPSTPAKALFGRVTGPAS
CCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCC
GPARPFGSYAKGCFSGGEALPLDGATWQVMRPSRNRMWGTPHLVDFIERLAARAPQAGWP
CCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
GLLVGDMSQPRGGPMLTGHASHQLGLDVDVWLTPMPDHRMTRAEREETSATDMVRSDRLD
EEEEECCCCCCCCCEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
IDPDVWTRQHTALIRMTAKQPEVARIFVNAAIKKALCREAGGDRSWLTKVRPMYGHNYHY
CCCCHHCCHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEE
HIRLACPAGDGACDDQAPPPSGDGCGSELDYWFSAAVLNPPKPKTPPKPRPPMTLAGLPD
EEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCCCCCEEECCCCH
ACRAVLHAR
HHHHHHHCC
>Mature Secondary Structure 
PLTRLTLPALALALPALLSPAAAQDRGSVNPKPLPPLANPNDPSTPAKALFGRVTGPAS
CCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCC
GPARPFGSYAKGCFSGGEALPLDGATWQVMRPSRNRMWGTPHLVDFIERLAARAPQAGWP
CCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
GLLVGDMSQPRGGPMLTGHASHQLGLDVDVWLTPMPDHRMTRAEREETSATDMVRSDRLD
EEEEECCCCCCCCCEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
IDPDVWTRQHTALIRMTAKQPEVARIFVNAAIKKALCREAGGDRSWLTKVRPMYGHNYHY
CCCCHHCCHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEE
HIRLACPAGDGACDDQAPPPSGDGCGSELDYWFSAAVLNPPKPKTPPKPRPPMTLAGLPD
EEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCCCCCEEECCCCH
ACRAVLHAR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]