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The map label for this gene is rsh [H]

Identifier: 218531452

GI number: 218531452

Start: 3704805

End: 3707036

Strand: Reverse

Name: rsh [H]

Synonym: Mchl_3520

Alternate gene names: 218531452

Gene position: 3707036-3704805 (Counterclockwise)

Preceding gene: 218531453

Following gene: 218531451

Centisome position: 64.16

GC content: 67.88

Gene sequence:

>2232_bases
ATGATGCGCCAGTACGAACTCGTCGAGCGGGTCAAGCGCTACAATCCCGCTGCGAACGAGGCGCTTCTCAACCGCGCCTA
CGTCTATGCCATGCGGGCGCACGGCACGCAGAAGCGCGCCTCCGGCGATCCGTTCTTCGCCCATCCGCTCGAAGTCGCCG
CGATCCTCACCGACCTGCGCCTCGACGACGCGACTATCGTCGCGGCTGTCCTGCACGACACCGTCGAGGACACCGCCGCT
ACGCTCGACGAGATCCGCGAGATCTTCGGGCCTGAGATCGGCGCGCTGGTGGACGGGCTGACCAAGCTCAAGCGCCTCGA
CCTCGTCTCGAAGCGGGCGGTGCAGGGCGAGAATTTCCGTAAGCTGCTCCTGGCGGTGGCCGAGGACGTGCGGGTGCTTC
TGGTGAAGCTCGCCGACCGCCTGCACAACATGCGCACGCTCCACTTCATGCGCGACGACAAGCGGGCACGGATTGCCGAG
GAGACCCTCGACATCTACGCGCCGCTCGCCGGCCGTATGGGCATGCAGGAACTGCGCGACGAGCTGGAAGACCTCTCGTT
CCGCACGCTAAAGCCTGAGGTCTACGCCACCATCACCAAGCGGCTCGAAGACCTCTCGGCGAAATCTGGCAGCGTGATCG
AGAGCATCGAGCACGACCTCACCGCCCGGCTCGCCGCGCGCGGCATCGCCGCTCACGTGAAAGGGCGGCTGAAGAAGCCG
TTCTCGATCTGGTCGAAGATGGAGCGCAAGTCGGTCGCCTTCGAGCAGCTCTCCGATATCGTCGGCTTCCGCGTGATCGT
CGGCACCCTGGCCGAGTGCTACGCGGCGCTCGGTGTGGTGCATACGAGTTGGCCGATGGTGCCGGGCCGCTACAAGGACT
ACGTCTCGACACCGAAGCAGAACGATTATCGCTCGATCCACACCACGGTGATCGGGCCGAAGCGCCAGCGCGTCGAGCTG
CAGATCCGCACCGCCGAGATGGACGAGATCGCCGAGTACGGCATCGCGGCCCACGCCCATTACAAAGAGGCCGGCAAAGA
GGGTGTGGAGGGCGAGGTCCACCCCCGGCTCGCCACCGAGAGCGGCGCCTACCAATGGCTGCGCCGCACCATCGAGCTTC
TGGCCGAGGGCGATTCGCCGGAAGAGTTTCTGGAGCACACTAAGCTGGAGCTGTTCCAGGATCAGGTGTTCTGCTTCACG
CCCAAGGGCCGCCTGATCGCCCTGCCGCGCGGCGCCACGCCGATCGACTTCGCCTACGCGGTGCATACCGATGTCGGCAA
CACGGCGGTGGGCGCCAAGATCAACGGCCGGCTCGCGCCGCTGCTGCACGAACTCGCCAACGGCGACGAGGTCGAGATCG
CCCGCTCCGACGGCGCCTCGCCGCCGGCCGCGTGGGAATCGCTCGTCGTCACCGGCAAGGCGCGGTCGGCGATCCGGCGG
GCGACCCGCGCCGCCGTGCGGCGCCAATATGCCGGTCTCGGCCGCCAGATCCTCGACCGGGCCTTCGAGCGGGCCGGCAA
GAGCTTCTCCGAGGAGAAGCTGCGCGGCGCGCTGCCCCGGCTCGCCCGCGCGAGCACCGAAGACGTGTTCGCCGCCGTCG
GACGCGGCGAGATGTTCTCCGGCGACGTGGTGAAGGCTGTCTATCCCGACTTCAAGGAGGAGCGGCGCGCGGGGGCGAGC
GGCGGTCCGGCGGCCAATGGCGCGGCGGGGCGCCTCACCCGCTCCAAGGATCAGACCATGCGGCTGACCTTCTCGGGCGA
GGCCGAGGCGGCCTCGGCCTTGGCCAGCATCCCGATTCGCGGACTGGCCGGCGACCTGCCGGTGAGCTTTGCCCCGAACG
GCGGCGCCCTGCCGGGGGATCGCATCGTCGGCATCCTCACGCCCGGCGTCGGCGTGACGATCTATCCGATCCAGTCCGCC
GCGCTCGCCGCCTTCGACAACGAGCCCGAGCGCTGGCTCGACGTGCGCTGGGATGTGGAGGGCTCGCAGGAGCGCTTCCC
CGCCAAGCTCGCGCTCGAATCGATCAACGAGCCCGGCACGCTGGCGCAGATCGCCCAGGTGATCGCCGAGCATGACGGCA
ACATCGACAATGTTTCGATGAAGCGTCGCACGCAGGACTTCACCGATATCTCGATTGATCTCTCGGTACCGGATCTGAAG
CATCTCACCGCCATCGTCGCGGACCTGCGCGGCAAGCGCTCGGTGAGCCGTGTCGAGCGAGTGAACGGCTGA

Upstream 100 bases:

>100_bases
GAAACGCCCGACCGCCGATCCTCGCCCCTCTGAGGCACGGCGGCGCGGCGGGCGTCTTGTGAGCGACACCGAGCAGATCG
AGAGCAGGATCCTTCGGCGG

Downstream 100 bases:

>100_bases
CGGTCGATCGCTGTCGGCGTCGCGTACCGGAACCGATCCCGGGACACGTCATTCCGTCATCGGATCGGCCTATGGACGGC
TGGGCCTGCGACGCGCTTTT

Product: (p)ppGpp synthetase I, SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase [H]

Number of amino acids: Translated: 743; Mature: 743

Protein sequence:

>743_residues
MMRQYELVERVKRYNPAANEALLNRAYVYAMRAHGTQKRASGDPFFAHPLEVAAILTDLRLDDATIVAAVLHDTVEDTAA
TLDEIREIFGPEIGALVDGLTKLKRLDLVSKRAVQGENFRKLLLAVAEDVRVLLVKLADRLHNMRTLHFMRDDKRARIAE
ETLDIYAPLAGRMGMQELRDELEDLSFRTLKPEVYATITKRLEDLSAKSGSVIESIEHDLTARLAARGIAAHVKGRLKKP
FSIWSKMERKSVAFEQLSDIVGFRVIVGTLAECYAALGVVHTSWPMVPGRYKDYVSTPKQNDYRSIHTTVIGPKRQRVEL
QIRTAEMDEIAEYGIAAHAHYKEAGKEGVEGEVHPRLATESGAYQWLRRTIELLAEGDSPEEFLEHTKLELFQDQVFCFT
PKGRLIALPRGATPIDFAYAVHTDVGNTAVGAKINGRLAPLLHELANGDEVEIARSDGASPPAAWESLVVTGKARSAIRR
ATRAAVRRQYAGLGRQILDRAFERAGKSFSEEKLRGALPRLARASTEDVFAAVGRGEMFSGDVVKAVYPDFKEERRAGAS
GGPAANGAAGRLTRSKDQTMRLTFSGEAEAASALASIPIRGLAGDLPVSFAPNGGALPGDRIVGILTPGVGVTIYPIQSA
ALAAFDNEPERWLDVRWDVEGSQERFPAKLALESINEPGTLAQIAQVIAEHDGNIDNVSMKRRTQDFTDISIDLSVPDLK
HLTAIVADLRGKRSVSRVERVNG

Sequences:

>Translated_743_residues
MMRQYELVERVKRYNPAANEALLNRAYVYAMRAHGTQKRASGDPFFAHPLEVAAILTDLRLDDATIVAAVLHDTVEDTAA
TLDEIREIFGPEIGALVDGLTKLKRLDLVSKRAVQGENFRKLLLAVAEDVRVLLVKLADRLHNMRTLHFMRDDKRARIAE
ETLDIYAPLAGRMGMQELRDELEDLSFRTLKPEVYATITKRLEDLSAKSGSVIESIEHDLTARLAARGIAAHVKGRLKKP
FSIWSKMERKSVAFEQLSDIVGFRVIVGTLAECYAALGVVHTSWPMVPGRYKDYVSTPKQNDYRSIHTTVIGPKRQRVEL
QIRTAEMDEIAEYGIAAHAHYKEAGKEGVEGEVHPRLATESGAYQWLRRTIELLAEGDSPEEFLEHTKLELFQDQVFCFT
PKGRLIALPRGATPIDFAYAVHTDVGNTAVGAKINGRLAPLLHELANGDEVEIARSDGASPPAAWESLVVTGKARSAIRR
ATRAAVRRQYAGLGRQILDRAFERAGKSFSEEKLRGALPRLARASTEDVFAAVGRGEMFSGDVVKAVYPDFKEERRAGAS
GGPAANGAAGRLTRSKDQTMRLTFSGEAEAASALASIPIRGLAGDLPVSFAPNGGALPGDRIVGILTPGVGVTIYPIQSA
ALAAFDNEPERWLDVRWDVEGSQERFPAKLALESINEPGTLAQIAQVIAEHDGNIDNVSMKRRTQDFTDISIDLSVPDLK
HLTAIVADLRGKRSVSRVERVNG
>Mature_743_residues
MMRQYELVERVKRYNPAANEALLNRAYVYAMRAHGTQKRASGDPFFAHPLEVAAILTDLRLDDATIVAAVLHDTVEDTAA
TLDEIREIFGPEIGALVDGLTKLKRLDLVSKRAVQGENFRKLLLAVAEDVRVLLVKLADRLHNMRTLHFMRDDKRARIAE
ETLDIYAPLAGRMGMQELRDELEDLSFRTLKPEVYATITKRLEDLSAKSGSVIESIEHDLTARLAARGIAAHVKGRLKKP
FSIWSKMERKSVAFEQLSDIVGFRVIVGTLAECYAALGVVHTSWPMVPGRYKDYVSTPKQNDYRSIHTTVIGPKRQRVEL
QIRTAEMDEIAEYGIAAHAHYKEAGKEGVEGEVHPRLATESGAYQWLRRTIELLAEGDSPEEFLEHTKLELFQDQVFCFT
PKGRLIALPRGATPIDFAYAVHTDVGNTAVGAKINGRLAPLLHELANGDEVEIARSDGASPPAAWESLVVTGKARSAIRR
ATRAAVRRQYAGLGRQILDRAFERAGKSFSEEKLRGALPRLARASTEDVFAAVGRGEMFSGDVVKAVYPDFKEERRAGAS
GGPAANGAAGRLTRSKDQTMRLTFSGEAEAASALASIPIRGLAGDLPVSFAPNGGALPGDRIVGILTPGVGVTIYPIQSA
ALAAFDNEPERWLDVRWDVEGSQERFPAKLALESINEPGTLAQIAQVIAEHDGNIDNVSMKRRTQDFTDISIDLSVPDLK
HLTAIVADLRGKRSVSRVERVNG

Specific function: Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in ada

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=132, Percent_Identity=39.3939393939394, Blast_Score=70, Evalue=8e-12,
Organism=Escherichia coli, GI1790082, Length=736, Percent_Identity=37.2282608695652, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1789147, Length=738, Percent_Identity=32.3848238482385, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25143118, Length=133, Percent_Identity=38.3458646616541, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24650996, Length=124, Percent_Identity=34.6774193548387, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 81655; Mature: 81655

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRQYELVERVKRYNPAANEALLNRAYVYAMRAHGTQKRASGDPFFAHPLEVAAILTDLR
CCCHHHHHHHHHHCCCCHHHHHHHHHHHEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHCC
LDDATIVAAVLHDTVEDTAATLDEIREIFGPEIGALVDGLTKLKRLDLVSKRAVQGENFR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KLLLAVAEDVRVLLVKLADRLHNMRTLHFMRDDKRARIAEETLDIYAPLAGRMGMQELRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
ELEDLSFRTLKPEVYATITKRLEDLSAKSGSVIESIEHDLTARLAARGIAAHVKGRLKKP
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FSIWSKMERKSVAFEQLSDIVGFRVIVGTLAECYAALGVVHTSWPMVPGRYKDYVSTPKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCC
NDYRSIHTTVIGPKRQRVELQIRTAEMDEIAEYGIAAHAHYKEAGKEGVEGEVHPRLATE
CCCCEEEHEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SGAYQWLRRTIELLAEGDSPEEFLEHTKLELFQDQVFCFTPKGRLIALPRGATPIDFAYA
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCEEEEE
VHTDVGNTAVGAKINGRLAPLLHELANGDEVEIARSDGASPPAAWESLVVTGKARSAIRR
EECCCCCCEECCEECCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHEEECHHHHHHHH
ATRAAVRRQYAGLGRQILDRAFERAGKSFSEEKLRGALPRLARASTEDVFAAVGRGEMFS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCC
GDVVKAVYPDFKEERRAGASGGPAANGAAGRLTRSKDQTMRLTFSGEAEAASALASIPIR
CCCHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCC
GLAGDLPVSFAPNGGALPGDRIVGILTPGVGVTIYPIQSAALAAFDNEPERWLDVRWDVE
CCCCCCCEEECCCCCCCCCCCEEEEECCCCCEEEEEECCHHHHHCCCCCCCEEEEEECCC
GSQERFPAKLALESINEPGTLAQIAQVIAEHDGNIDNVSMKRRTQDFTDISIDLSVPDLK
CCHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCHH
HLTAIVADLRGKRSVSRVERVNG
HHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MMRQYELVERVKRYNPAANEALLNRAYVYAMRAHGTQKRASGDPFFAHPLEVAAILTDLR
CCCHHHHHHHHHHCCCCHHHHHHHHHHHEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHCC
LDDATIVAAVLHDTVEDTAATLDEIREIFGPEIGALVDGLTKLKRLDLVSKRAVQGENFR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KLLLAVAEDVRVLLVKLADRLHNMRTLHFMRDDKRARIAEETLDIYAPLAGRMGMQELRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
ELEDLSFRTLKPEVYATITKRLEDLSAKSGSVIESIEHDLTARLAARGIAAHVKGRLKKP
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FSIWSKMERKSVAFEQLSDIVGFRVIVGTLAECYAALGVVHTSWPMVPGRYKDYVSTPKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCC
NDYRSIHTTVIGPKRQRVELQIRTAEMDEIAEYGIAAHAHYKEAGKEGVEGEVHPRLATE
CCCCEEEHEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SGAYQWLRRTIELLAEGDSPEEFLEHTKLELFQDQVFCFTPKGRLIALPRGATPIDFAYA
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCEEEEE
VHTDVGNTAVGAKINGRLAPLLHELANGDEVEIARSDGASPPAAWESLVVTGKARSAIRR
EECCCCCCEECCEECCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHEEECHHHHHHHH
ATRAAVRRQYAGLGRQILDRAFERAGKSFSEEKLRGALPRLARASTEDVFAAVGRGEMFS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCC
GDVVKAVYPDFKEERRAGASGGPAANGAAGRLTRSKDQTMRLTFSGEAEAASALASIPIR
CCCHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCC
GLAGDLPVSFAPNGGALPGDRIVGILTPGVGVTIYPIQSAALAAFDNEPERWLDVRWDVE
CCCCCCCEEECCCCCCCCCCCEEEEECCCCCEEEEEECCHHHHHCCCCCCCEEEEEECCC
GSQERFPAKLALESINEPGTLAQIAQVIAEHDGNIDNVSMKRRTQDFTDISIDLSVPDLK
CCHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCHH
HLTAIVADLRGKRSVSRVERVNG
HHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA