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The map label for this gene is yjeA [H]

Identifier: 218531179

GI number: 218531179

Start: 3411221

End: 3412279

Strand: Reverse

Name: yjeA [H]

Synonym: Mchl_3229

Alternate gene names: 218531179

Gene position: 3412279-3411221 (Counterclockwise)

Preceding gene: 218531181

Following gene: 218531178

Centisome position: 59.06

GC content: 70.35

Gene sequence:

>1059_bases
GTGAGTCACGAGTCTTCGCCCTGGTGGCATCCCGGCCTCCATGCGGATCGCCGGCCGCTGCTGCTCACCCGCAACCGCAT
CCTCGCGGGTTTTCGCGCGTGGTTTGCCGAGCGCGATTTCGTTGAGGTCGAAGCCGCCGCGCTGCAAGTCTCCCCCGGCA
ACGAGGCGCATCTCTCGGCCTTCGCCACGGAGGCGATTCTGCCCGATGGTGCGCGCGAGCCGCTCTACCTCCACACCTCG
CCGGAATTCGCCTGCAAGAAGCTGCTGGCGGCGGGCGAGCCGCGCCTGTTCAGCCTCGCTCACGTCTTCCGCAACCGCGA
GCGCGGCGCGCTGCACCATCCCGAATTCACGATGCTCGAATGGTACCGGGCGGGCGAGAGCTACGACGCGCTGATGCAGG
ACGGCGCCGCGCTACTCGCGCTCGCCGCCGAGACGGCGGGCGTGCGACGCCTGACCTTTCGCGGGCGCGAGGCCGATCCG
TTCGTACCCTTCGAGCGGCTGACCCTGGCGGAGGCGTTCCAGCGCTATGCAGCCATCGATCTGCTCGCCACGGTTTCGCC
CGATGGCGGCACCGACCGGGATACACTGGCCGACGCGGTGATGACCCGCGGCCTGCGAGTCGCCCCGGACGACACCTGGG
CCGACCTGTTCAGCCGCGTCCTCGTCGGGCTGATCGAGCCGCATCTCGGCCAGGGGCGCCCGACCATCCTGTGCGAGTAT
CCAGTGAGCGAGGCGGCGCTCGCCCGGCCGAGCCCGCGTGACCCGCGCGTCGCCGAACGCTTCGAGCTCTATGCCTGCGG
CGTCGAACTCGCCAACGCCTTCGGCGAACTCACCGATCCGGCCGAGCAGCGCCGTCGCTTCGAGGCGGAGATGGCGGAGA
AGGCCCGCGTCTACGGCGAGCGCTACCCGATCGACGAGGAGTTCCTGGACGCGCTGGCGATCATGCCCGAGGCGAGCGGC
ATCGCGCTCGGCTTCGACCGGCTGGTGATGCTCGCCACCGGCGCGCCCCGCATCGACGATGTGATCTGGACACCGGTCCC
CCGGAACCGGTCGCGGTGA

Upstream 100 bases:

>100_bases
GAACGGCGGCTCTTCACCGGCGTCTCGGCCGGATGAGCGGAATCATCCGCGAGGCCCTACCCTATCCGCGGCTCGGTTGC
CAGCCGGAGGCTGTTTTCGC

Downstream 100 bases:

>100_bases
GCATCGCCCTCAAGAGTGCCACCGCGCTGGCCCGCGCCGGGCTGGTGGACGCAGCTGCCCTCCCCGCCCTGGAGCGGGTC
GCGGCGCGCTACGCCGTCTC

Product: tRNA synthetase class II (D K and N)

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSHESSPWWHPGLHADRRPLLLTRNRILAGFRAWFAERDFVEVEAAALQVSPGNEAHLSAFATEAILPDGAREPLYLHTS
PEFACKKLLAAGEPRLFSLAHVFRNRERGALHHPEFTMLEWYRAGESYDALMQDGAALLALAAETAGVRRLTFRGREADP
FVPFERLTLAEAFQRYAAIDLLATVSPDGGTDRDTLADAVMTRGLRVAPDDTWADLFSRVLVGLIEPHLGQGRPTILCEY
PVSEAALARPSPRDPRVAERFELYACGVELANAFGELTDPAEQRRRFEAEMAEKARVYGERYPIDEEFLDALAIMPEASG
IALGFDRLVMLATGAPRIDDVIWTPVPRNRSR

Sequences:

>Translated_352_residues
MSHESSPWWHPGLHADRRPLLLTRNRILAGFRAWFAERDFVEVEAAALQVSPGNEAHLSAFATEAILPDGAREPLYLHTS
PEFACKKLLAAGEPRLFSLAHVFRNRERGALHHPEFTMLEWYRAGESYDALMQDGAALLALAAETAGVRRLTFRGREADP
FVPFERLTLAEAFQRYAAIDLLATVSPDGGTDRDTLADAVMTRGLRVAPDDTWADLFSRVLVGLIEPHLGQGRPTILCEY
PVSEAALARPSPRDPRVAERFELYACGVELANAFGELTDPAEQRRRFEAEMAEKARVYGERYPIDEEFLDALAIMPEASG
IALGFDRLVMLATGAPRIDDVIWTPVPRNRSR
>Mature_351_residues
SHESSPWWHPGLHADRRPLLLTRNRILAGFRAWFAERDFVEVEAAALQVSPGNEAHLSAFATEAILPDGAREPLYLHTSP
EFACKKLLAAGEPRLFSLAHVFRNRERGALHHPEFTMLEWYRAGESYDALMQDGAALLALAAETAGVRRLTFRGREADPF
VPFERLTLAEAFQRYAAIDLLATVSPDGGTDRDTLADAVMTRGLRVAPDDTWADLFSRVLVGLIEPHLGQGRPTILCEYP
VSEAALARPSPRDPRVAERFELYACGVELANAFGELTDPAEQRRRFEAEMAEKARVYGERYPIDEEFLDALAIMPEASGI
ALGFDRLVMLATGAPRIDDVIWTPVPRNRSR

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=337, Percent_Identity=27.893175074184, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI5031815, Length=337, Percent_Identity=27.893175074184, Blast_Score=132, Evalue=4e-31,
Organism=Escherichia coli, GI87082379, Length=355, Percent_Identity=38.3098591549296, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1790571, Length=333, Percent_Identity=33.033033033033, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1789256, Length=333, Percent_Identity=32.7327327327327, Blast_Score=156, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71994340, Length=342, Percent_Identity=29.2397660818713, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17535927, Length=342, Percent_Identity=29.2397660818713, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17535925, Length=342, Percent_Identity=29.2397660818713, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320242, Length=344, Percent_Identity=27.3255813953488, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640851, Length=342, Percent_Identity=26.0233918128655, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24640849, Length=342, Percent_Identity=26.0233918128655, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 39247; Mature: 39116

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHESSPWWHPGLHADRRPLLLTRNRILAGFRAWFAERDFVEVEAAALQVSPGNEAHLSA
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHH
FATEAILPDGAREPLYLHTSPEFACKKLLAAGEPRLFSLAHVFRNRERGALHHPEFTMLE
HHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
WYRAGESYDALMQDGAALLALAAETAGVRRLTFRGREADPFVPFERLTLAEAFQRYAAID
HHHCCCHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
LLATVSPDGGTDRDTLADAVMTRGLRVAPDDTWADLFSRVLVGLIEPHLGQGRPTILCEY
HHEEECCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
PVSEAALARPSPRDPRVAERFELYACGVELANAFGELTDPAEQRRRFEAEMAEKARVYGE
CCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
RYPIDEEFLDALAIMPEASGIALGFDRLVMLATGAPRIDDVIWTPVPRNRSR
CCCCCHHHHHHHHHCCCCCCCEECHHHHHEEECCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
SHESSPWWHPGLHADRRPLLLTRNRILAGFRAWFAERDFVEVEAAALQVSPGNEAHLSA
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHH
FATEAILPDGAREPLYLHTSPEFACKKLLAAGEPRLFSLAHVFRNRERGALHHPEFTMLE
HHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
WYRAGESYDALMQDGAALLALAAETAGVRRLTFRGREADPFVPFERLTLAEAFQRYAAID
HHHCCCHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
LLATVSPDGGTDRDTLADAVMTRGLRVAPDDTWADLFSRVLVGLIEPHLGQGRPTILCEY
HHEEECCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
PVSEAALARPSPRDPRVAERFELYACGVELANAFGELTDPAEQRRRFEAEMAEKARVYGE
CCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
RYPIDEEFLDALAIMPEASGIALGFDRLVMLATGAPRIDDVIWTPVPRNRSR
CCCCCHHHHHHHHHCCCCCCCEECHHHHHEEECCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]