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The map label for this gene is tyrS [H]

Identifier: 218531163

GI number: 218531163

Start: 3396072

End: 3397343

Strand: Reverse

Name: tyrS [H]

Synonym: Mchl_3213

Alternate gene names: 218531163

Gene position: 3397343-3396072 (Counterclockwise)

Preceding gene: 218531168

Following gene: 218531160

Centisome position: 58.8

GC content: 66.19

Gene sequence:

>1272_bases
ATGACCGCCGAGAGCTTCGCGCCGAAATCCGACTTCATCCGGGTGCTGCAGGAGCGCGGCTACATCCATCAGGCCTCCGA
CCTTGCCGGCATCGACGCCCTGGCGGCGGAAGGGGGGCTGACCACCTATACCGGCTACGATTGCACGGCGGCCTCGCTCC
ATGTCGGCCACCTGCTGTCGATCATGATGCTGCATTGGCTGCAGAAGACCGGCGGCAAGCCGGTCGTGCTGATGGGCGGG
GGCACCACCCGCGTCGGCGATCCTTCGGGGCGCGACGAGACGCGCAAGATCCTGACGCTGGAGCAGATCGAGGCCAACAA
GGAAGGCATCAAGCAGACGTTTTCGCGCTTCCTCTCCTTCGGTGAGGGCGGAAACGGCGCGGTCATGGTCGACAACGCCG
AGTGGCTGACGAAGCTCAACTATATCGAGATGCTGCGCGACATCGGCCGGCATTTCTCGGTCAACCGCATGATGTCGATG
GACAGCGTGCGGATGCGGCTGGAGCGCGACCAGGAGCTGTCGTTCCTCGAATTCAACTACATGATCCTGCAGTCCTACGA
CTTCGTGGAGCTGAACCGCCGCTTCGGCACCCGCCTGCAGATGGGCGGGTCCGACCAGTGGGGCAACATCGTCAACGGCA
TCGATCTCGGCCGCCGCATGGGCACGCCGCAGCTCTACGCCCTGACCTGCCCGCTCCTGACCACGTCGTCGGGCGCCAAG
ATGGGCAAGACTGCCGCCGGCGCCGTGTGGCTCGATGCCGGGATGCTCTCGCCTTACGATTACTGGCAATTCTGGCGGAA
CACCGAGGATGCCGATGTCGGCCGCTTCCTTAAGCTGTTCACGCTGTTGCCGCTACCCGAGATCGAACGCCTCGCCGCGC
TTCAGGGCGCCGAGATCAACGAGGCCAAGACGGTCCTCGCCACGGAAGCGACGGCGCTGATGCATGGCCCGGACGCCGCC
GCCGCCGCCGCCGAAACCGCCCGGAAAACCTTCGTCGAGGGCACGCTGGCGCAGTCCCTGCCGACGATTCCCGTGCCGCC
CGCGGTGCTTGAGGCGGGCCTCGGCGTGCTCACCGCCTTCGGGCCGGACTACGCCAAGCTCGTCCCTTCGACCAGCGAGG
CGCGCCGCCAGATCAAGGGCGGCGGCCTGCGGGTCAACGACGTGCAGGTGAGCGACGAGAAGGCGGTGCTTCGCAAAGAA
GACCTGACGGCCGAGGGCGTGATCAAGCTCTCGTTCGGGCGCAAGAAGCACGTGCTGCTGCGGCCGGAATAG

Upstream 100 bases:

>100_bases
CGGCCTGCCCTGCTAAGAGCGCGCGCGAGCGCTTCCCCTCGCGGGATAGCATTGCGGCCGCGCCCCTGTCGCGCCGCCAA
ACCATCCGAGACCGATCACC

Downstream 100 bases:

>100_bases
CTGTTCCGCCCAGCCGGTAACGGGGGCTCCGCACCGCGGGCCCCTGATCGATCCGCGTTCCGCCCTCAGGCGGCCTGAAG
CCCGCGCACTCGGGCGAAGC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTAESFAPKSDFIRVLQERGYIHQASDLAGIDALAAEGGLTTYTGYDCTAASLHVGHLLSIMMLHWLQKTGGKPVVLMGG
GTTRVGDPSGRDETRKILTLEQIEANKEGIKQTFSRFLSFGEGGNGAVMVDNAEWLTKLNYIEMLRDIGRHFSVNRMMSM
DSVRMRLERDQELSFLEFNYMILQSYDFVELNRRFGTRLQMGGSDQWGNIVNGIDLGRRMGTPQLYALTCPLLTTSSGAK
MGKTAAGAVWLDAGMLSPYDYWQFWRNTEDADVGRFLKLFTLLPLPEIERLAALQGAEINEAKTVLATEATALMHGPDAA
AAAAETARKTFVEGTLAQSLPTIPVPPAVLEAGLGVLTAFGPDYAKLVPSTSEARRQIKGGGLRVNDVQVSDEKAVLRKE
DLTAEGVIKLSFGRKKHVLLRPE

Sequences:

>Translated_423_residues
MTAESFAPKSDFIRVLQERGYIHQASDLAGIDALAAEGGLTTYTGYDCTAASLHVGHLLSIMMLHWLQKTGGKPVVLMGG
GTTRVGDPSGRDETRKILTLEQIEANKEGIKQTFSRFLSFGEGGNGAVMVDNAEWLTKLNYIEMLRDIGRHFSVNRMMSM
DSVRMRLERDQELSFLEFNYMILQSYDFVELNRRFGTRLQMGGSDQWGNIVNGIDLGRRMGTPQLYALTCPLLTTSSGAK
MGKTAAGAVWLDAGMLSPYDYWQFWRNTEDADVGRFLKLFTLLPLPEIERLAALQGAEINEAKTVLATEATALMHGPDAA
AAAAETARKTFVEGTLAQSLPTIPVPPAVLEAGLGVLTAFGPDYAKLVPSTSEARRQIKGGGLRVNDVQVSDEKAVLRKE
DLTAEGVIKLSFGRKKHVLLRPE
>Mature_422_residues
TAESFAPKSDFIRVLQERGYIHQASDLAGIDALAAEGGLTTYTGYDCTAASLHVGHLLSIMMLHWLQKTGGKPVVLMGGG
TTRVGDPSGRDETRKILTLEQIEANKEGIKQTFSRFLSFGEGGNGAVMVDNAEWLTKLNYIEMLRDIGRHFSVNRMMSMD
SVRMRLERDQELSFLEFNYMILQSYDFVELNRRFGTRLQMGGSDQWGNIVNGIDLGRRMGTPQLYALTCPLLTTSSGAKM
GKTAAGAVWLDAGMLSPYDYWQFWRNTEDADVGRFLKLFTLLPLPEIERLAALQGAEINEAKTVLATEATALMHGPDAAA
AAAETARKTFVEGTLAQSLPTIPVPPAVLEAGLGVLTAFGPDYAKLVPSTSEARRQIKGGGLRVNDVQVSDEKAVLRKED
LTAEGVIKLSFGRKKHVLLRPE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=401, Percent_Identity=33.6658354114713, Blast_Score=223, Evalue=4e-58,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=40.3301886792453, Blast_Score=313, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI25145802, Length=295, Percent_Identity=32.2033898305085, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25145805, Length=208, Percent_Identity=32.2115384615385, Blast_Score=121, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6325160, Length=399, Percent_Identity=33.5839598997494, Blast_Score=207, Evalue=4e-54,
Organism=Drosophila melanogaster, GI20130383, Length=434, Percent_Identity=35.0230414746544, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46352; Mature: 46221

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAESFAPKSDFIRVLQERGYIHQASDLAGIDALAAEGGLTTYTGYDCTAASLHVGHLLS
CCCCCCCCHHHHHHHHHHCCCEECCCCCCCHHHHHCCCCCEEECCCCCCHHHHHHHHHHH
IMMLHWLQKTGGKPVVLMGGGTTRVGDPSGRDETRKILTLEQIEANKEGIKQTFSRFLSF
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHEEHHHHHCCHHHHHHHHHHHHHC
GEGGNGAVMVDNAEWLTKLNYIEMLRDIGRHFSVNRMMSMDSVRMRLERDQELSFLEFNY
CCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEE
MILQSYDFVELNRRFGTRLQMGGSDQWGNIVNGIDLGRRMGTPQLYALTCPLLTTSSGAK
EEEECCCHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCEECCCCCC
MGKTAAGAVWLDAGMLSPYDYWQFWRNTEDADVGRFLKLFTLLPLPEIERLAALQGAEIN
CCCCCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
EAKTVLATEATALMHGPDAAAAAAETARKTFVEGTLAQSLPTIPVPPAVLEAGLGVLTAF
HHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHEECC
GPDYAKLVPSTSEARRQIKGGGLRVNDVQVSDEKAVLRKEDLTAEGVIKLSFGRKKHVLL
CCCHHHHCCCHHHHHHHHCCCCEEEEEEEECCHHHHHHHCCCCCCCEEEEECCCCCCEEE
RPE
CCC
>Mature Secondary Structure 
TAESFAPKSDFIRVLQERGYIHQASDLAGIDALAAEGGLTTYTGYDCTAASLHVGHLLS
CCCCCCCHHHHHHHHHHCCCEECCCCCCCHHHHHCCCCCEEECCCCCCHHHHHHHHHHH
IMMLHWLQKTGGKPVVLMGGGTTRVGDPSGRDETRKILTLEQIEANKEGIKQTFSRFLSF
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHEEHHHHHCCHHHHHHHHHHHHHC
GEGGNGAVMVDNAEWLTKLNYIEMLRDIGRHFSVNRMMSMDSVRMRLERDQELSFLEFNY
CCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEE
MILQSYDFVELNRRFGTRLQMGGSDQWGNIVNGIDLGRRMGTPQLYALTCPLLTTSSGAK
EEEECCCHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCEECCCCCC
MGKTAAGAVWLDAGMLSPYDYWQFWRNTEDADVGRFLKLFTLLPLPEIERLAALQGAEIN
CCCCCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
EAKTVLATEATALMHGPDAAAAAAETARKTFVEGTLAQSLPTIPVPPAVLEAGLGVLTAF
HHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHEECC
GPDYAKLVPSTSEARRQIKGGGLRVNDVQVSDEKAVLRKEDLTAEGVIKLSFGRKKHVLL
CCCHHHHCCCHHHHHHHHCCCCEEEEEEEECCHHHHHHHCCCCCCCEEEEECCCCCCEEE
RPE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA