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The map label for this gene is tsf [H]

Identifier: 218236064

GI number: 218236064

Start: 3845803

End: 3846690

Strand: Reverse

Name: tsf [H]

Synonym: BCB4264_A3924

Alternate gene names: 218236064

Gene position: 3846690-3845803 (Counterclockwise)

Preceding gene: 218235460

Following gene: 218233853

Centisome position: 70.98

GC content: 40.09

Gene sequence:

>888_bases
ATGGCAATCACTGCTCAAATGGTAAAAGAATTACGTGAAAAAACTGGCGCAGGTATGATGGACTGCAAAAAAGCTTTAAC
AGAAACTAACGGCGACATGGAGAAGGCAATTGACTTCTTACGTGAAAAAGGTATTGCGAAAGCTGCTAAAAAAGCAGACC
GCATTGCTGCTGAAGGTTTAACTTTCATCGAAACAAACGGTAACGACGCTTTAATCTTAGAATTAAACTCTGAAACTGAT
TTCGTTGCGAAAAACGAAGGTTTCCAAACACTAATTAAAGAATTAGCTGCTCACTTATTAACTAACAAACCAGCTAACGT
TGAAGAAGCTATGGCTCAAACAATGGAAAACGGTAAAAAAGTTGAAGAGCACATCAACGAAGCAATCGCTAAAATTGGTG
AAAAACTTACACTTCGTCGTTTCGAAATCGTATCAAAAACTGATGCAGATGCATTCGGCGCTTACCTACACATGGGTGGA
CGCATTGGTGTATTAACAGTTCTTGAAGGTTCTACTGATGAAGCTGCTGCTAAAGATGTTGCAATGCACATCGCTGCAGT
TAACCCTAAATACATCGACCGCGATGCTGTAACAGCTGAAGAAGTTGAGCATGAGCGTCAAGTATTAACACAACAAGCAT
TAAACGAAGGCAAGCCTGAGAAAATCGTTGCGAAAATGGTTGAAGGCCGTCTAGGCAAATTCTTCGAAGAGATTTGCTTA
CTTGACCAAGCATTCGTTAAAAACCCTGATATGAAAGTTCGTCAATTCGTTGAGTCTAAAGGCGGAACATTAAAAGGATT
CGTTCGCTACGCTGTTGGTGAAGGTATCGAAAAACGCGAAGACAACTTTGCTGAAGAAGTAATGAACCAAGTAAAAGGTA
GTAACTAA

Upstream 100 bases:

>100_bases
AGTTGGAAAGGTGATAAGGGGTTCGGCCTTTTATCACCTTTTTTAAGGTATAAACACCTTTATAAAAGGTATAAATACAT
ATTTTAGGAGGATGAAAAGT

Downstream 100 bases:

>100_bases
TACAGATTAGGGAACACATTGTGTTCCCTTTTTTAAAATAAAGATGTAAGCAATTGGAGGTTCATTATGAGTAAACCGAA
ATATAATCGTGTCGTTTTAA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAITAQMVKELREKTGAGMMDCKKALTETNGDMEKAIDFLREKGIAKAAKKADRIAAEGLTFIETNGNDALILELNSETD
FVAKNEGFQTLIKELAAHLLTNKPANVEEAMAQTMENGKKVEEHINEAIAKIGEKLTLRRFEIVSKTDADAFGAYLHMGG
RIGVLTVLEGSTDEAAAKDVAMHIAAVNPKYIDRDAVTAEEVEHERQVLTQQALNEGKPEKIVAKMVEGRLGKFFEEICL
LDQAFVKNPDMKVRQFVESKGGTLKGFVRYAVGEGIEKREDNFAEEVMNQVKGSN

Sequences:

>Translated_295_residues
MAITAQMVKELREKTGAGMMDCKKALTETNGDMEKAIDFLREKGIAKAAKKADRIAAEGLTFIETNGNDALILELNSETD
FVAKNEGFQTLIKELAAHLLTNKPANVEEAMAQTMENGKKVEEHINEAIAKIGEKLTLRRFEIVSKTDADAFGAYLHMGG
RIGVLTVLEGSTDEAAAKDVAMHIAAVNPKYIDRDAVTAEEVEHERQVLTQQALNEGKPEKIVAKMVEGRLGKFFEEICL
LDQAFVKNPDMKVRQFVESKGGTLKGFVRYAVGEGIEKREDNFAEEVMNQVKGSN
>Mature_294_residues
AITAQMVKELREKTGAGMMDCKKALTETNGDMEKAIDFLREKGIAKAAKKADRIAAEGLTFIETNGNDALILELNSETDF
VAKNEGFQTLIKELAAHLLTNKPANVEEAMAQTMENGKKVEEHINEAIAKIGEKLTLRRFEIVSKTDADAFGAYLHMGGR
IGVLTVLEGSTDEAAAKDVAMHIAAVNPKYIDRDAVTAEEVEHERQVLTQQALNEGKPEKIVAKMVEGRLGKFFEEICLL
DQAFVKNPDMKVRQFVESKGGTLKGFVRYAVGEGIEKREDNFAEEVMNQVKGSN

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=303, Percent_Identity=24.4224422442244, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI291084500, Length=324, Percent_Identity=24.0740740740741, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI291084498, Length=124, Percent_Identity=33.8709677419355, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI291084502, Length=99, Percent_Identity=38.3838383838384, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1786366, Length=292, Percent_Identity=46.9178082191781, Blast_Score=234, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI17561440, Length=300, Percent_Identity=26.6666666666667, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921466, Length=146, Percent_Identity=35.6164383561644, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 32480; Mature: 32348

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITAQMVKELREKTGAGMMDCKKALTETNGDMEKAIDFLREKGIAKAAKKADRIAAEGL
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TFIETNGNDALILELNSETDFVAKNEGFQTLIKELAAHLLTNKPANVEEAMAQTMENGKK
EEEEECCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHH
VEEHINEAIAKIGEKLTLRRFEIVSKTDADAFGAYLHMGGRIGVLTVLEGSTDEAAAKDV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHH
AMHIAAVNPKYIDRDAVTAEEVEHERQVLTQQALNEGKPEKIVAKMVEGRLGKFFEEICL
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LDQAFVKNPDMKVRQFVESKGGTLKGFVRYAVGEGIEKREDNFAEEVMNQVKGSN
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AITAQMVKELREKTGAGMMDCKKALTETNGDMEKAIDFLREKGIAKAAKKADRIAAEGL
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TFIETNGNDALILELNSETDFVAKNEGFQTLIKELAAHLLTNKPANVEEAMAQTMENGKK
EEEEECCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHH
VEEHINEAIAKIGEKLTLRRFEIVSKTDADAFGAYLHMGGRIGVLTVLEGSTDEAAAKDV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHH
AMHIAAVNPKYIDRDAVTAEEVEHERQVLTQQALNEGKPEKIVAKMVEGRLGKFFEEICL
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LDQAFVKNPDMKVRQFVESKGGTLKGFVRYAVGEGIEKREDNFAEEVMNQVKGSN
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA