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The map label for this gene is yhfS [H]

Identifier: 218236003

GI number: 218236003

Start: 3651146

End: 3652237

Strand: Reverse

Name: yhfS [H]

Synonym: BCB4264_A3735

Alternate gene names: 218236003

Gene position: 3652237-3651146 (Counterclockwise)

Preceding gene: 218232742

Following gene: 218231231

Centisome position: 67.4

GC content: 40.57

Gene sequence:

>1092_bases
ATGAATAGAGCAGTTATTGTAGAGGCGAAAAGAACACCTATTGGTAAGAAGAATGGGATGTTAAAAGAGTATGAAGTTCA
GCAATTAACAACGCCGCTTCTTACGTTTTTAAGTAAAGGGATTGAGAGGGAAATAGATGATGTCATATTAGGTAATGTTG
TTGGGCCAGGAGGGAATGTTGCGAGACTATCTGCTTTAGAGGCGGGACTTGGCTATCATATTCCTGGTGTAACGATTGAT
CGGCAATGTGGTGCTGGTTTAGAAGCAATTCGAACTGCGTGTCATCTCATTCAAGGCGGGGCAGGTAAGTGTTATATCAC
CGGGGGAGTAGAGAGTACAAGTACGTCACCTTTTCAAAAAAGAGCACGATTTTCACCTGAAACAATTGGTGATCCTGACA
TGGGAGTAGCGGCTGAATATGTTGCGGAGCGTTATAATATAACGAGAGAAATGCAAGACGAGTATGCTTGTCTTAGTTAT
AAGCGGACACTGCAAGCGTTAGAAAAAGGATATATACATGATGAAATATTTTCTTTTAATGGATTATTAGATGAATCTAT
AAAGCCAGAAATGAATTATGAACGAATCATTAAAAGAACAAAGCCCGCATTTTTACAAAATGGAACAGTGACGGCAGGTA
ATTCATGTGGTGTAAATGATGGGGCATGTGCTGTTCTTGTAATGGAAGAGGGACAAGCCCGAAAGTTAGGGTATAAACCT
GTCCTTCGTTTCGTTCGTAGTGCTGTAGTGGGAGTGGATCCTAATCTTCCAGGGACTGGTCCGATATTTGCGGTGAACAA
ATTATTAAACGAAATGAATGTAAAAGTAGAGGACATCGATTATTTTGAAATAAATGAAGCATTCGCCTCAAAAGTTGTTG
CTTGCGCAAAAGAGTTACAAATTCCGTACGAAAAAGTAAATGTAAATGGTGGAGCAATTGCGCTCGGTCATCCGTACGGC
GCATCTGGTGCTATGCTTGTAACACGTTTGTTTTATCAGGCACAAAGAGAGTCTATGAAATATGGGATTGCTACATTAGG
AATTGGAGGCGGGATAGGGATTGCGCTATTATTTGAGAAAGTAGAAGACTAG

Upstream 100 bases:

>100_bases
CCGAAGGAATGGCATTTTGTAGATGGAATACCTTATACAAATAGTGGGAAAATCGCTCGTATGGAAGCAAAAAGTATGAT
TGAAAATCGGGAGAAAATAT

Downstream 100 bases:

>100_bases
CCAGGTAACTAGTTTTCTACTTTTTGAGGAACATAAACATTACTACCAGGCTTCGCTTTCATTTCGTTCCAAGCAGCAAT
CGCATCAGCTTTTGCTTTCC

Product: acetyl-CoA acetyltransferase

Products: CoA; acetoacetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MNRAVIVEAKRTPIGKKNGMLKEYEVQQLTTPLLTFLSKGIEREIDDVILGNVVGPGGNVARLSALEAGLGYHIPGVTID
RQCGAGLEAIRTACHLIQGGAGKCYITGGVESTSTSPFQKRARFSPETIGDPDMGVAAEYVAERYNITREMQDEYACLSY
KRTLQALEKGYIHDEIFSFNGLLDESIKPEMNYERIIKRTKPAFLQNGTVTAGNSCGVNDGACAVLVMEEGQARKLGYKP
VLRFVRSAVVGVDPNLPGTGPIFAVNKLLNEMNVKVEDIDYFEINEAFASKVVACAKELQIPYEKVNVNGGAIALGHPYG
ASGAMLVTRLFYQAQRESMKYGIATLGIGGGIGIALLFEKVED

Sequences:

>Translated_363_residues
MNRAVIVEAKRTPIGKKNGMLKEYEVQQLTTPLLTFLSKGIEREIDDVILGNVVGPGGNVARLSALEAGLGYHIPGVTID
RQCGAGLEAIRTACHLIQGGAGKCYITGGVESTSTSPFQKRARFSPETIGDPDMGVAAEYVAERYNITREMQDEYACLSY
KRTLQALEKGYIHDEIFSFNGLLDESIKPEMNYERIIKRTKPAFLQNGTVTAGNSCGVNDGACAVLVMEEGQARKLGYKP
VLRFVRSAVVGVDPNLPGTGPIFAVNKLLNEMNVKVEDIDYFEINEAFASKVVACAKELQIPYEKVNVNGGAIALGHPYG
ASGAMLVTRLFYQAQRESMKYGIATLGIGGGIGIALLFEKVED
>Mature_363_residues
MNRAVIVEAKRTPIGKKNGMLKEYEVQQLTTPLLTFLSKGIEREIDDVILGNVVGPGGNVARLSALEAGLGYHIPGVTID
RQCGAGLEAIRTACHLIQGGAGKCYITGGVESTSTSPFQKRARFSPETIGDPDMGVAAEYVAERYNITREMQDEYACLSY
KRTLQALEKGYIHDEIFSFNGLLDESIKPEMNYERIIKRTKPAFLQNGTVTAGNSCGVNDGACAVLVMEEGQARKLGYKP
VLRFVRSAVVGVDPNLPGTGPIFAVNKLLNEMNVKVEDIDYFEINEAFASKVVACAKELQIPYEKVNVNGGAIALGHPYG
ASGAMLVTRLFYQAQRESMKYGIATLGIGGGIGIALLFEKVED

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=389, Percent_Identity=36.5038560411311, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI167614485, Length=387, Percent_Identity=34.1085271317829, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI4501853, Length=387, Percent_Identity=35.6589147286822, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI4557237, Length=391, Percent_Identity=33.5038363171356, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI4504327, Length=418, Percent_Identity=30.1435406698565, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI194353979, Length=362, Percent_Identity=28.4530386740331, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI48994986, Length=388, Percent_Identity=37.3711340206186, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788554, Length=397, Percent_Identity=39.5465994962217, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=38.0597014925373, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082165, Length=397, Percent_Identity=37.27959697733, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=26.8292682926829, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI133906874, Length=392, Percent_Identity=36.9897959183673, Blast_Score=223, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=32.824427480916, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25147385, Length=388, Percent_Identity=30.9278350515464, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=29.0094339622642, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17535917, Length=388, Percent_Identity=27.5773195876289, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322031, Length=389, Percent_Identity=34.7043701799486, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6325229, Length=392, Percent_Identity=32.1428571428571, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24655093, Length=394, Percent_Identity=38.8324873096447, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI17648125, Length=390, Percent_Identity=37.6923076923077, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=32.6478149100257, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI17137578, Length=420, Percent_Identity=29.7619047619048, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.9

Molecular weight: Translated: 39297; Mature: 39297

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00737 THIOLASE_2 ; PS00139 THIOL_PROTEASE_CYS ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRAVIVEAKRTPIGKKNGMLKEYEVQQLTTPLLTFLSKGIEREIDDVILGNVVGPGGNV
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
ARLSALEAGLGYHIPGVTIDRQCGAGLEAIRTACHLIQGGAGKCYITGGVESTSTSPFQK
HHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
RARFSPETIGDPDMGVAAEYVAERYNITREMQDEYACLSYKRTLQALEKGYIHDEIFSFN
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
GLLDESIKPEMNYERIIKRTKPAFLQNGTVTAGNSCGVNDGACAVLVMEEGQARKLGYKP
CCCCCCCCCCCCHHHHHHHCCCHHCCCCCEECCCCCCCCCCCEEEEEECCCCCCCCCHHH
VLRFVRSAVVGVDPNLPGTGPIFAVNKLLNEMNVKVEDIDYFEINEAFASKVVACAKELQ
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHC
IPYEKVNVNGGAIALGHPYGASGAMLVTRLFYQAQRESMKYGIATLGIGGGIGIALLFEK
CCHHHEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHH
VED
HCC
>Mature Secondary Structure
MNRAVIVEAKRTPIGKKNGMLKEYEVQQLTTPLLTFLSKGIEREIDDVILGNVVGPGGNV
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
ARLSALEAGLGYHIPGVTIDRQCGAGLEAIRTACHLIQGGAGKCYITGGVESTSTSPFQK
HHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
RARFSPETIGDPDMGVAAEYVAERYNITREMQDEYACLSYKRTLQALEKGYIHDEIFSFN
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
GLLDESIKPEMNYERIIKRTKPAFLQNGTVTAGNSCGVNDGACAVLVMEEGQARKLGYKP
CCCCCCCCCCCCHHHHHHHCCCHHCCCCCEECCCCCCCCCCCEEEEEECCCCCCCCCHHH
VLRFVRSAVVGVDPNLPGTGPIFAVNKLLNEMNVKVEDIDYFEINEAFASKVVACAKELQ
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHC
IPYEKVNVNGGAIALGHPYGASGAMLVTRLFYQAQRESMKYGIATLGIGGGIGIALLFEK
CCHHHEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHH
VED
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA

Specific reaction: 2 acetyl-CoA = CoA + acetoacetyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]