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The map label for this gene is yhfT [H]

Identifier: 218232742

GI number: 218232742

Start: 3652230

End: 3653693

Strand: Reverse

Name: yhfT [H]

Synonym: BCB4264_A3736

Alternate gene names: 218232742

Gene position: 3653693-3652230 (Counterclockwise)

Preceding gene: 218232129

Following gene: 218236003

Centisome position: 67.42

GC content: 33.88

Gene sequence:

>1464_bases
ATGGGGATTACAAAAGAATATAAAAAGCATGCCTCTTTACAACCAAATAAAATAGCGATAAAGGAAAATGATAGAGTTTT
AACATATAAAGAGTGGTTTGAATCAGTTTGTAAAGTAGCAAGCTGGTTAAATGAAAAAAAATCGAAGAATAAAACGATAG
CAATCCTATTAGAAAATCGTATAGAGTTTTTACAACTATTTTCTGGTGCCGCTATGGCCGGGTGGGTTTGTGTTCCGTTA
GATATAAAGTGGAAACAAGATGAGCTCAGAGAAAGAATTGAAATTAGTAATCCGGATATGATTGTGACAGAACGATATAA
GGTGAATGATCTATTGGATGAAGAAGGAAGAGTAATTGAAATTGATGAGTGGAAAAGAATGATAGAGAAATATCTTCCTA
CATATTCCACTGTAGAAAGTGTACAACATGCTCCTTTTTATATGGGATTTACATCAGGATCAACAGGAACAGCAAAAGCA
TTTTTACGTGCACAGCAATCGTGGGTTCATAGTTTTGATTGTAATGTACATGACTTTCATATGAAAAGGGAGGATTCAAT
TTTAATAGCTGGGACGCTCGTTCATTCTCTTTTCTTATACGGGGCAATAAGTGCATTATATGTAGGACAAACAGTGCACA
TTATGAGAAAGTTCATTCCGAATCAAGTATTAGATAAGTTAGAAACTGAAAATATATCGGTTATGTATACAGTTCCGACA
ATGCTTGAATCTTTATATAAAGAAAATAGATTAATAGAAAATAAAATGAAAATTATTTCATCAGGAGCGAAATGGGAAGT
TGAAGCGAAAGAAAAAATAAAGAATATATTTCCTTATGCGAAAAGATATGAATTTTATGGTGCATCAGAGTTAAGTTTTG
TAACAGCACTAGTCGATGCAGAAAGCGAAAGAAGGCCAAATTCAGTAGGAAAACCTTGTCATAATGTGCAAGTTCGAATA
TGTAATGAAGTAGGCGAAGAAGTACAAAAAGGTGAAATAGGAACTGTTTATGTGAAAAGTGATCAGTTTTTTATGGGATA
CATAATAGATGGGGTTTTAACACGGGAGTTGAATGCAGAAGGCTGGATGACAGTGCGAGATATAGGCTATGAAGATGAAG
AAGGCTTCATATATATTGTTGGTAGAGAGAAGAATATGATTTTATTTGGAGGAATCAATATTTATCCAGAAGAAATAGAA
AGTGTATTACATGAACACCCAGCTGTTGATGAAATAGTTGTAATTGGTGTGAAAGATAGTTACTGGGGTGAAAAGCCGGT
CGCGATCGTAAAAGGAAGTGCTACAAAACAACAATTAAAGAGTTTTTGCTTACAACGATTATCCTCGTTTAAAATACCGA
AGGAATGGCATTTTGTAGATGGAATACCTTATACAAATAGTGGGAAAATCGCTCGTATGGAAGCAAAAAGTATGATTGAA
AATCGGGAGAAAATATATGAATAG

Upstream 100 bases:

>100_bases
TTTAAAGAATGGAAAATATACAAATGCGGGATGATAAAGGGAAGAAACACGTTCATTGCGTGTTTCTTTTTATTATTTTT
GTAAAGAAAGGACGTCTCAT

Downstream 100 bases:

>100_bases
AGCAGTTATTGTAGAGGCGAAAAGAACACCTATTGGTAAGAAGAATGGGATGTTAAAAGAGTATGAAGTTCAGCAATTAA
CAACGCCGCTTCTTACGTTT

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVCKVASWLNEKKSKNKTIAILLENRIEFLQLFSGAAMAGWVCVPL
DIKWKQDELRERIEISNPDMIVTERYKVNDLLDEEGRVIEIDEWKRMIEKYLPTYSTVESVQHAPFYMGFTSGSTGTAKA
FLRAQQSWVHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLETENISVMYTVPT
MLESLYKENRLIENKMKIISSGAKWEVEAKEKIKNIFPYAKRYEFYGASELSFVTALVDAESERRPNSVGKPCHNVQVRI
CNEVGEEVQKGEIGTVYVKSDQFFMGYIIDGVLTRELNAEGWMTVRDIGYEDEEGFIYIVGREKNMILFGGINIYPEEIE
SVLHEHPAVDEIVVIGVKDSYWGEKPVAIVKGSATKQQLKSFCLQRLSSFKIPKEWHFVDGIPYTNSGKIARMEAKSMIE
NREKIYE

Sequences:

>Translated_487_residues
MGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVCKVASWLNEKKSKNKTIAILLENRIEFLQLFSGAAMAGWVCVPL
DIKWKQDELRERIEISNPDMIVTERYKVNDLLDEEGRVIEIDEWKRMIEKYLPTYSTVESVQHAPFYMGFTSGSTGTAKA
FLRAQQSWVHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLETENISVMYTVPT
MLESLYKENRLIENKMKIISSGAKWEVEAKEKIKNIFPYAKRYEFYGASELSFVTALVDAESERRPNSVGKPCHNVQVRI
CNEVGEEVQKGEIGTVYVKSDQFFMGYIIDGVLTRELNAEGWMTVRDIGYEDEEGFIYIVGREKNMILFGGINIYPEEIE
SVLHEHPAVDEIVVIGVKDSYWGEKPVAIVKGSATKQQLKSFCLQRLSSFKIPKEWHFVDGIPYTNSGKIARMEAKSMIE
NREKIYE
>Mature_486_residues
GITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVCKVASWLNEKKSKNKTIAILLENRIEFLQLFSGAAMAGWVCVPLD
IKWKQDELRERIEISNPDMIVTERYKVNDLLDEEGRVIEIDEWKRMIEKYLPTYSTVESVQHAPFYMGFTSGSTGTAKAF
LRAQQSWVHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLETENISVMYTVPTM
LESLYKENRLIENKMKIISSGAKWEVEAKEKIKNIFPYAKRYEFYGASELSFVTALVDAESERRPNSVGKPCHNVQVRIC
NEVGEEVQKGEIGTVYVKSDQFFMGYIIDGVLTRELNAEGWMTVRDIGYEDEEGFIYIVGREKNMILFGGINIYPEEIES
VLHEHPAVDEIVVIGVKDSYWGEKPVAIVKGSATKQQLKSFCLQRLSSFKIPKEWHFVDGIPYTNSGKIARMEAKSMIEN
REKIYE

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=358, Percent_Identity=28.7709497206704, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI187761345, Length=381, Percent_Identity=28.6089238845144, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI187761343, Length=381, Percent_Identity=28.6089238845144, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI157311624, Length=481, Percent_Identity=23.9085239085239, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI157311622, Length=481, Percent_Identity=23.9085239085239, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI58082049, Length=464, Percent_Identity=24.1379310344828, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI42544132, Length=283, Percent_Identity=27.5618374558304, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI122937307, Length=363, Percent_Identity=26.9972451790634, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI115511026, Length=472, Percent_Identity=23.9406779661017, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI38505220, Length=463, Percent_Identity=23.110151187905, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI13375727, Length=545, Percent_Identity=21.8348623853211, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788107, Length=529, Percent_Identity=25.1417769376182, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI145693145, Length=511, Percent_Identity=23.2876712328767, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786810, Length=516, Percent_Identity=23.062015503876, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI221142682, Length=468, Percent_Identity=23.7179487179487, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788595, Length=338, Percent_Identity=27.2189349112426, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1790505, Length=423, Percent_Identity=22.2222222222222, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI32563687, Length=486, Percent_Identity=25.3086419753086, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=529, Percent_Identity=24.1965973534972, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=300, Percent_Identity=31, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=507, Percent_Identity=23.4714003944773, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=359, Percent_Identity=27.0194986072423, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=351, Percent_Identity=27.6353276353276, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=351, Percent_Identity=27.6353276353276, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=351, Percent_Identity=27.6353276353276, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=494, Percent_Identity=23.6842105263158, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=363, Percent_Identity=27.8236914600551, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=349, Percent_Identity=26.3610315186246, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=495, Percent_Identity=23.6363636363636, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71996755, Length=485, Percent_Identity=23.7113402061856, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25141359, Length=489, Percent_Identity=22.4948875255624, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25141361, Length=272, Percent_Identity=22.4264705882353, Blast_Score=65, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6319699, Length=526, Percent_Identity=24.7148288973384, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=546, Percent_Identity=24.7252747252747, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24581924, Length=402, Percent_Identity=27.363184079602, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI21355181, Length=486, Percent_Identity=23.8683127572016, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859661, Length=498, Percent_Identity=24.4979919678715, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21356947, Length=346, Percent_Identity=25.1445086705202, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=23.0769230769231, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356441, Length=463, Percent_Identity=23.3261339092873, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648255, Length=518, Percent_Identity=22.972972972973, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648260, Length=340, Percent_Identity=25.8823529411765, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21358303, Length=250, Percent_Identity=28, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24653035, Length=341, Percent_Identity=24.0469208211144, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281365686, Length=181, Percent_Identity=28.7292817679558, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI161076582, Length=348, Percent_Identity=24.7126436781609, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922652, Length=476, Percent_Identity=20.7983193277311, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648257, Length=504, Percent_Identity=21.031746031746, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130357, Length=206, Percent_Identity=26.6990291262136, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24656500, Length=344, Percent_Identity=24.7093023255814, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24667955, Length=384, Percent_Identity=25.5208333333333, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24667959, Length=384, Percent_Identity=25.5208333333333, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI62472339, Length=384, Percent_Identity=25.5208333333333, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 56092; Mature: 55961

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVCKVASWLNEKKSKNKTIAILLENR
CCCCHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
IEFLQLFSGAAMAGWVCVPLDIKWKQDELRERIEISNPDMIVTERYKVNDLLDEEGRVIE
HHHHHHHCCCHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEEHHHHHCCCCCEEE
IDEWKRMIEKYLPTYSTVESVQHAPFYMGFTSGSTGTAKAFLRAQQSWVHSFDCNVHDFH
HHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
MKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLETENISVMYTVPT
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
MLESLYKENRLIENKMKIISSGAKWEVEAKEKIKNIFPYAKRYEFYGASELSFVTALVDA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCHHHHEECCCCCHHHHHHHHHCC
ESERRPNSVGKPCHNVQVRICNEVGEEVQKGEIGTVYVKSDQFFMGYIIDGVLTRELNAE
CCCCCCCCCCCCCHHHHEEHHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHCCCCC
GWMTVRDIGYEDEEGFIYIVGREKNMILFGGINIYPEEIESVLHEHPAVDEIVVIGVKDS
CCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCC
YWGEKPVAIVKGSATKQQLKSFCLQRLSSFKIPKEWHFVDGIPYTNSGKIARMEAKSMIE
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEHHHHHHHH
NREKIYE
HHHHHCC
>Mature Secondary Structure 
GITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVCKVASWLNEKKSKNKTIAILLENR
CCCHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
IEFLQLFSGAAMAGWVCVPLDIKWKQDELRERIEISNPDMIVTERYKVNDLLDEEGRVIE
HHHHHHHCCCHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEEHHHHHCCCCCEEE
IDEWKRMIEKYLPTYSTVESVQHAPFYMGFTSGSTGTAKAFLRAQQSWVHSFDCNVHDFH
HHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
MKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLETENISVMYTVPT
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
MLESLYKENRLIENKMKIISSGAKWEVEAKEKIKNIFPYAKRYEFYGASELSFVTALVDA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCHHHHEECCCCCHHHHHHHHHCC
ESERRPNSVGKPCHNVQVRICNEVGEEVQKGEIGTVYVKSDQFFMGYIIDGVLTRELNAE
CCCCCCCCCCCCCHHHHEEHHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHCCCCC
GWMTVRDIGYEDEEGFIYIVGREKNMILFGGINIYPEEIESVLHEHPAVDEIVVIGVKDS
CCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCC
YWGEKPVAIVKGSATKQQLKSFCLQRLSSFKIPKEWHFVDGIPYTNSGKIARMEAKSMIE
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEHHHHHHHH
NREKIYE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]