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The map label for this gene is 218235772

Identifier: 218235772

GI number: 218235772

Start: 2926810

End: 2927847

Strand: Reverse

Name: 218235772

Synonym: BCB4264_A3050

Alternate gene names: NA

Gene position: 2927847-2926810 (Counterclockwise)

Preceding gene: 218235436

Following gene: 218234223

Centisome position: 54.03

GC content: 38.73

Gene sequence:

>1038_bases
ATGAAAATGTTAATACTAGGTGGTACACGATTTTTGGGAAGAGCTGTGGTAGAAGAGGCTTTGAAAAGAGGGCATGAAGT
TACATTATTTAACCGCGGAACAAACAAAGAAGTTTTTCCTGAAGTGGAGCAGCTGATCGGTGACAGAAATGGCGATGTAT
CAAGTTTAGAAAATCGAAAATGGGATGTTGTTGTGGATACATGTGGGTTTTCTCCGCATCACATTAGAAATGTTGGAGAA
GTACTTAAAGATAATATTGAACACTATATATTCATCTCAAGCCTTTCCGTATATAAAGATTGGATTCCGCATGACATAAA
AGAAGACTATATATTACAACCTGAACCGACGGATGATCAAATAAAGGCTGTAGAGAATGGTGAAGTTTCACCTTATGAGT
ATTATGGTGCACTGAAAGTATTATGTGAAAAAGAAGCAGAGAAGTATTGGCCGGGGCGTGTATTACACGTAAGAGCAGGA
CTTCTTTCAGGAATGTTTGATTATACAGATCGACTTCCATACTGGATCGGGCGTGTAGCAAAGGGTGGGAAAGTATTAGT
ACCAGGAAGAAAAATTCGTCCCGTGCAATTAGTTGATATAAAAGATGTCGCGAGATGGGGGCTAAATATGGCAGAACATA
ATAAAGCAGGAACATTTAATATAACAGGTCCGAATGATGAATTGACGATGGAAGAGCTATTAAATACGTGTAAAAAGGTT
ACGAATAGCGATGCTGAATTTGTTTGGGTAGACGAATCATTTATGAGTGAACATAATGTGCAGCCTTGGACAGAAATGCC
TTTGTGGATTCCAGAAACTTTTCCATTAGATGGTGAGACGAAGCCGTGGAGAGGCGGATTTTCTATTTGTATCGAAAGTG
CGGTTAATGAAGGGCTTACTTTTAGAAGAATAGAAGAGACAGTTACAGATGTGTATGAGTGGATGGAGAGTACGGAAGAA
TGGGAATTAAAAGCAGGTATTTCAGGGGGAAGAGAAAAGGTGTTATTGGATAAGTGGTATGAAATAAAGAGAGTTTAA

Upstream 100 bases:

>100_bases
TATTGAACTTGTAGAAAAAATTTAATTATCCCCGTAACTTTTCACAACAAAAAATCGTTAAACAAAAGAATAGATTTTTT
ATCACGAATGGGGGAGAACC

Downstream 100 bases:

>100_bases
ATAAGAATCTTGTGAAAAAAGGACTCTGTTTATTGGTTAAACAGAGTCCTAATATGAATCTACCACTCAATCGGTTCTTC
CCCGTACATCTCCCATAGCT

Product: isoflavone reductase

Products: 2'-hydroxyformononetin; NADPH; H+

Alternate protein names: Reductase; Isoflavone Reductase; NAD Dependent Epimerase/Dehydratase Family Protein; Nucleoside-Diphosphate-Sugar Epimerase; NAD-Dependent Epimerase/Dehydratase Family Protein; NAD Dependent Epimerase/Dehydratase; Nucleotide Sugar Epimerase; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase Family; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase; Oxidoreductase; DTDP-Glucose 4 6-Dehydratase; NmrA Family Protein; Sugar Dehydratase; Dtdpglucose 4 6-Dehydratase-Like Protein; NAD-Dependent Protein; NAD Dependent Epimerase; NAD Dependent Epimerase/Dehydratase Family

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKMLILGGTRFLGRAVVEEALKRGHEVTLFNRGTNKEVFPEVEQLIGDRNGDVSSLENRKWDVVVDTCGFSPHHIRNVGE
VLKDNIEHYIFISSLSVYKDWIPHDIKEDYILQPEPTDDQIKAVENGEVSPYEYYGALKVLCEKEAEKYWPGRVLHVRAG
LLSGMFDYTDRLPYWIGRVAKGGKVLVPGRKIRPVQLVDIKDVARWGLNMAEHNKAGTFNITGPNDELTMEELLNTCKKV
TNSDAEFVWVDESFMSEHNVQPWTEMPLWIPETFPLDGETKPWRGGFSICIESAVNEGLTFRRIEETVTDVYEWMESTEE
WELKAGISGGREKVLLDKWYEIKRV

Sequences:

>Translated_345_residues
MKMLILGGTRFLGRAVVEEALKRGHEVTLFNRGTNKEVFPEVEQLIGDRNGDVSSLENRKWDVVVDTCGFSPHHIRNVGE
VLKDNIEHYIFISSLSVYKDWIPHDIKEDYILQPEPTDDQIKAVENGEVSPYEYYGALKVLCEKEAEKYWPGRVLHVRAG
LLSGMFDYTDRLPYWIGRVAKGGKVLVPGRKIRPVQLVDIKDVARWGLNMAEHNKAGTFNITGPNDELTMEELLNTCKKV
TNSDAEFVWVDESFMSEHNVQPWTEMPLWIPETFPLDGETKPWRGGFSICIESAVNEGLTFRRIEETVTDVYEWMESTEE
WELKAGISGGREKVLLDKWYEIKRV
>Mature_345_residues
MKMLILGGTRFLGRAVVEEALKRGHEVTLFNRGTNKEVFPEVEQLIGDRNGDVSSLENRKWDVVVDTCGFSPHHIRNVGE
VLKDNIEHYIFISSLSVYKDWIPHDIKEDYILQPEPTDDQIKAVENGEVSPYEYYGALKVLCEKEAEKYWPGRVLHVRAG
LLSGMFDYTDRLPYWIGRVAKGGKVLVPGRKIRPVQLVDIKDVARWGLNMAEHNKAGTFNITGPNDELTMEELLNTCKKV
TNSDAEFVWVDESFMSEHNVQPWTEMPLWIPETFPLDGETKPWRGGFSICIESAVNEGLTFRRIEETVTDVYEWMESTEE
WELKAGISGGREKVLLDKWYEIKRV

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.3.1.45

Molecular weight: Translated: 39654; Mature: 39654

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMLILGGTRFLGRAVVEEALKRGHEVTLFNRGTNKEVFPEVEQLIGDRNGDVSSLENRK
CEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHCCCC
WDVVVDTCGFSPHHIRNVGEVLKDNIEHYIFISSLSVYKDWIPHDIKEDYILQPEPTDDQ
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHCCCCCCCCEEECCCCCCHH
IKAVENGEVSPYEYYGALKVLCEKEAEKYWPGRVLHVRAGLLSGMFDYTDRLPYWIGRVA
HEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHC
KGGKVLVPGRKIRPVQLVDIKDVARWGLNMAEHNKAGTFNITGPNDELTMEELLNTCKKV
CCCEEEECCCCCCCEEEECHHHHHHHCCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHH
TNSDAEFVWVDESFMSEHNVQPWTEMPLWIPETFPLDGETKPWRGGFSICIESAVNEGLT
CCCCCCEEEECHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
FRRIEETVTDVYEWMESTEEWELKAGISGGREKVLLDKWYEIKRV
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHHCC
>Mature Secondary Structure
MKMLILGGTRFLGRAVVEEALKRGHEVTLFNRGTNKEVFPEVEQLIGDRNGDVSSLENRK
CEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHCCCC
WDVVVDTCGFSPHHIRNVGEVLKDNIEHYIFISSLSVYKDWIPHDIKEDYILQPEPTDDQ
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHCCCCCCCCEEECCCCCCHH
IKAVENGEVSPYEYYGALKVLCEKEAEKYWPGRVLHVRAGLLSGMFDYTDRLPYWIGRVA
HEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHC
KGGKVLVPGRKIRPVQLVDIKDVARWGLNMAEHNKAGTFNITGPNDELTMEELLNTCKKV
CCCEEEECCCCCCCEEEECHHHHHHHCCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHH
TNSDAEFVWVDESFMSEHNVQPWTEMPLWIPETFPLDGETKPWRGGFSICIESAVNEGLT
CCCCCCEEEECHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
FRRIEETVTDVYEWMESTEEWELKAGISGGREKVLLDKWYEIKRV
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vestitone; NADP+

Specific reaction: vestitone + NADP+ = 2'-hydroxyformononetin + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA