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The map label for this gene is nylB [H]

Identifier: 218235436

GI number: 218235436

Start: 2929050

End: 2929991

Strand: Reverse

Name: nylB [H]

Synonym: BCB4264_A3052

Alternate gene names: 218235436

Gene position: 2929991-2929050 (Counterclockwise)

Preceding gene: 218234759

Following gene: 218235772

Centisome position: 54.07

GC content: 36.52

Gene sequence:

>942_bases
ATGAAAACTGCTGAAAAGTTAGATCAAATTATACAAGAAGAATATAAAAATATGAATGGCATGTTAGTAGTGCAGAAAGG
TAATGTTGTTTTTGAAAATTATTATAATGGTTACAGTCCAGATAATGCGTTTCATGTAGCGTCAGTTACAAAAACGATAA
TCTCTGCGTTAATTGGGATATGTATAGATAAGGGTTATATAAAAAGTGTTGATCAAAAAGTAATAGAATTTTTCCCGGAA
TATAAATGTAAGGAATCTGAAGTAACCGTACGCCATCTTCTAACAATGACAGCGCCATATCCTTTTGTAGACTGGCAGGA
ACCGTTAGAAGAACTATGTACACAAGAGGATTGGGTACAGCATACACTTAATAGAATGGGGAACGGTGGAGAAATTGGAT
CTTTTAAATACTCATCTGCAGGAGCGCATGTACTATCAGCAATTATTACGAATGCAACAGGGAAAAGTGCACGTGAATTT
GCGAATGAGTACTTATTTCAACCACTCGGCATGAGCGAAATTCCAAACTACAATATGAAAGCATTTGGATTCGATGACTT
ATTCGGAAAAGATGTGAAAGGATGGGTTCACGATCCAAATGGTATTTCGACGGGCGGCTGGGGAGTAACGTTAACGGTTA
GGGATATGGCTAAGTTTGGACAGATGTATTTGAATGAAGGTATTCATAATGGAAAACAAGTTCTATCAAAATCATGGATA
AAAGAATCAATAGCGATGAATTCAAATCAATATGGTTACTTATGGTGGTTACGAGAAGAAGAGGGTGTCTATTCGTATTG
TGCAATGGGTGATGGTGGGAATATGATTTGTTGTATTCCGAAGCAGGATTTGGTTGTAGTAATTGCTTCTGAAGCTAAGC
CGAATGCACGGGATAGATGGGAATTAATTAAAGGGTGCATACTTCCTTATTTAAAAGATTAA

Upstream 100 bases:

>100_bases
ATATAGAAATGATCCAAAGCAGCACCCGAAAAATAAACATATAGTTGATATATATGTACCTATCGAACCTTTTTAATTAT
AGGTTAAAGGGGAAGGAATA

Downstream 100 bases:

>100_bases
TATGGTTATTTTTATAAAAATAAGAATGAAAGGTTTAGTTAAACTTGTTTTGTTCTCCATGATAGAAAAAAGTGTTGTGT
TAACAAAGTTATGTAACTAA

Product: beta-lactamase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKTAEKLDQIIQEEYKNMNGMLVVQKGNVVFENYYNGYSPDNAFHVASVTKTIISALIGICIDKGYIKSVDQKVIEFFPE
YKCKESEVTVRHLLTMTAPYPFVDWQEPLEELCTQEDWVQHTLNRMGNGGEIGSFKYSSAGAHVLSAIITNATGKSAREF
ANEYLFQPLGMSEIPNYNMKAFGFDDLFGKDVKGWVHDPNGISTGGWGVTLTVRDMAKFGQMYLNEGIHNGKQVLSKSWI
KESIAMNSNQYGYLWWLREEEGVYSYCAMGDGGNMICCIPKQDLVVVIASEAKPNARDRWELIKGCILPYLKD

Sequences:

>Translated_313_residues
MKTAEKLDQIIQEEYKNMNGMLVVQKGNVVFENYYNGYSPDNAFHVASVTKTIISALIGICIDKGYIKSVDQKVIEFFPE
YKCKESEVTVRHLLTMTAPYPFVDWQEPLEELCTQEDWVQHTLNRMGNGGEIGSFKYSSAGAHVLSAIITNATGKSAREF
ANEYLFQPLGMSEIPNYNMKAFGFDDLFGKDVKGWVHDPNGISTGGWGVTLTVRDMAKFGQMYLNEGIHNGKQVLSKSWI
KESIAMNSNQYGYLWWLREEEGVYSYCAMGDGGNMICCIPKQDLVVVIASEAKPNARDRWELIKGCILPYLKD
>Mature_313_residues
MKTAEKLDQIIQEEYKNMNGMLVVQKGNVVFENYYNGYSPDNAFHVASVTKTIISALIGICIDKGYIKSVDQKVIEFFPE
YKCKESEVTVRHLLTMTAPYPFVDWQEPLEELCTQEDWVQHTLNRMGNGGEIGSFKYSSAGAHVLSAIITNATGKSAREF
ANEYLFQPLGMSEIPNYNMKAFGFDDLFGKDVKGWVHDPNGISTGGWGVTLTVRDMAKFGQMYLNEGIHNGKQVLSKSWI
KESIAMNSNQYGYLWWLREEEGVYSYCAMGDGGNMICCIPKQDLVVVIASEAKPNARDRWELIKGCILPYLKD

Specific function: This Protein Is A Serine Beta-Lactamase With A Substrate Specificity For Cephalosporins. [C]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 35321; Mature: 35321

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAEKLDQIIQEEYKNMNGMLVVQKGNVVFENYYNGYSPDNAFHVASVTKTIISALIGI
CCHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
CIDKGYIKSVDQKVIEFFPEYKCKESEVTVRHLLTMTAPYPFVDWQEPLEELCTQEDWVQ
HHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHH
HTLNRMGNGGEIGSFKYSSAGAHVLSAIITNATGKSAREFANEYLFQPLGMSEIPNYNMK
HHHHHCCCCCCCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCE
AFGFDDLFGKDVKGWVHDPNGISTGGWGVTLTVRDMAKFGQMYLNEGIHNGKQVLSKSWI
EECCHHHHCCCCCCCEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KESIAMNSNQYGYLWWLREEEGVYSYCAMGDGGNMICCIPKQDLVVVIASEAKPNARDRW
HHHHHCCCCCEEEEEEEECCCCCEEEEEECCCCCEEEEECCCCEEEEEECCCCCCCHHHH
ELIKGCILPYLKD
HHHHHHHHHHCCC
>Mature Secondary Structure
MKTAEKLDQIIQEEYKNMNGMLVVQKGNVVFENYYNGYSPDNAFHVASVTKTIISALIGI
CCHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
CIDKGYIKSVDQKVIEFFPEYKCKESEVTVRHLLTMTAPYPFVDWQEPLEELCTQEDWVQ
HHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHH
HTLNRMGNGGEIGSFKYSSAGAHVLSAIITNATGKSAREFANEYLFQPLGMSEIPNYNMK
HHHHHCCCCCCCCCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCE
AFGFDDLFGKDVKGWVHDPNGISTGGWGVTLTVRDMAKFGQMYLNEGIHNGKQVLSKSWI
EECCHHHHCCCCCCCEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KESIAMNSNQYGYLWWLREEEGVYSYCAMGDGGNMICCIPKQDLVVVIASEAKPNARDRW
HHHHHCCCCCEEEEEEEECCCCCEEEEEECCCCCEEEEECCCCEEEEEECCCCCCCHHHH
ELIKGCILPYLKD
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 2512123 [H]