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The map label for this gene is ilvE1 [H]

Identifier: 218235632

GI number: 218235632

Start: 1390257

End: 1391153

Strand: Direct

Name: ilvE1 [H]

Synonym: BCB4264_A1449

Alternate gene names: 218235632

Gene position: 1390257-1391153 (Clockwise)

Preceding gene: 218235470

Following gene: 218232047

Centisome position: 25.66

GC content: 35.79

Gene sequence:

>897_bases
GTGAATGAGCAATGGATTTTCTTAAATGGAGAATTTGTTCCAAAAGATGAGGCAAAAGTTTCAGTATATGATCATGGCTA
TTTATATGGCGATGGAGTGTTTGAAGGAATTAGAGTTTATAGCGGTAATGTTTTCCGTTTGAGAGAGCATCTTGTCCGGT
TGTATGAATCAGCAAAATCAATCCTTTTAGAAATTCCATATTCATTAGATGAAGTAACAAATATTGTTGTTGAGACGATT
CGACAAAATAAATTATCTAATGGATACATTCGCCTTGTTGTATCAAGAGGAGCAGGAAATCTTGGATTGGATCCCGATTC
ATGTAAGAAGCCGAATGTAGTAGTAATTGCAGAACAATTATCTTTATTCCCACAAGAATATTATGAGAAAGGGATTCCAG
TTGTAACAGTTGCAACGCGCCGAAATCGTCCCGATGTTTTATCCCCTCAAGTAAAATCTTTAAATTACTTAAACAATATT
TTAGTTCGTATTGAAGCGAAATTAGCTGGAGTACAAGAAGCGCTTATGTTAAATGATCAAGGATATGTAGCTGAAGGATC
AGGAGATAACGTGTTTATTGTTAAAGGAAATAAATTAATTACACCACCAAGTTCTGCTGGAGCGTTAGAAGGTATTACAC
GAAACGCCATTTTAGAAATCGGCGAAAAACTTGGGTATGACGTAAGAGAAGAGTTATTTACAAGACATGATGTATATGTA
GCTGATGAAGTGTTTTTAACAGGAACAGCTGCTGAAGTGATTGCTGTTACGACAGTAGATGGTAGAACGATTGGCTTAGG
GCAAACAGGTCCACATACGAACCGTTTATTAGAAGAGTTTCGTAAGTTAGTTGTAGAAGATGGAGAGAAAATTTACGAAG
AAAATAAAGTTGGATAA

Upstream 100 bases:

>100_bases
TTAAAAATTATAAATTTTCTAAAAATTCACTTGATTTATTTAGAAAGGAGAGTAAACTAGGAAACAATAAAAAATTCATA
GAACATAGGAGGCGCTTCTA

Downstream 100 bases:

>100_bases
CAACTTTTTTAACATAAGAAAGCGTTGATAGGGAGGAGTAGTTGATTGTGAAGCCTCACAGAGAGTCGGTGGTTGCTGAA
AACCGATGGTTCACGTCGAC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MNEQWIFLNGEFVPKDEAKVSVYDHGYLYGDGVFEGIRVYSGNVFRLREHLVRLYESAKSILLEIPYSLDEVTNIVVETI
RQNKLSNGYIRLVVSRGAGNLGLDPDSCKKPNVVVIAEQLSLFPQEYYEKGIPVVTVATRRNRPDVLSPQVKSLNYLNNI
LVRIEAKLAGVQEALMLNDQGYVAEGSGDNVFIVKGNKLITPPSSAGALEGITRNAILEIGEKLGYDVREELFTRHDVYV
ADEVFLTGTAAEVIAVTTVDGRTIGLGQTGPHTNRLLEEFRKLVVEDGEKIYEENKVG

Sequences:

>Translated_298_residues
MNEQWIFLNGEFVPKDEAKVSVYDHGYLYGDGVFEGIRVYSGNVFRLREHLVRLYESAKSILLEIPYSLDEVTNIVVETI
RQNKLSNGYIRLVVSRGAGNLGLDPDSCKKPNVVVIAEQLSLFPQEYYEKGIPVVTVATRRNRPDVLSPQVKSLNYLNNI
LVRIEAKLAGVQEALMLNDQGYVAEGSGDNVFIVKGNKLITPPSSAGALEGITRNAILEIGEKLGYDVREELFTRHDVYV
ADEVFLTGTAAEVIAVTTVDGRTIGLGQTGPHTNRLLEEFRKLVVEDGEKIYEENKVG
>Mature_298_residues
MNEQWIFLNGEFVPKDEAKVSVYDHGYLYGDGVFEGIRVYSGNVFRLREHLVRLYESAKSILLEIPYSLDEVTNIVVETI
RQNKLSNGYIRLVVSRGAGNLGLDPDSCKKPNVVVIAEQLSLFPQEYYEKGIPVVTVATRRNRPDVLSPQVKSLNYLNNI
LVRIEAKLAGVQEALMLNDQGYVAEGSGDNVFIVKGNKLITPPSSAGALEGITRNAILEIGEKLGYDVREELFTRHDVYV
ADEVFLTGTAAEVIAVTTVDGRTIGLGQTGPHTNRLLEEFRKLVVEDGEKIYEENKVG

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=299, Percent_Identity=38.7959866220736, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1787338, Length=249, Percent_Identity=25.3012048192771, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33119; Mature: 33119

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQWIFLNGEFVPKDEAKVSVYDHGYLYGDGVFEGIRVYSGNVFRLREHLVRLYESAKS
CCCCEEEECCEECCCCCCEEEEEECCEEEECCHHCCEEEECCCHHHHHHHHHHHHHHHHH
ILLEIPYSLDEVTNIVVETIRQNKLSNGYIRLVVSRGAGNLGLDPDSCKKPNVVVIAEQL
EEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHH
SLFPQEYYEKGIPVVTVATRRNRPDVLSPQVKSLNYLNNILVRIEAKLAGVQEALMLNDQ
HCCHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEHHHHCHHHHEEECCC
GYVAEGSGDNVFIVKGNKLITPPSSAGALEGITRNAILEIGEKLGYDVREELFTRHDVYV
CEEEECCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEE
ADEVFLTGTAAEVIAVTTVDGRTIGLGQTGPHTNRLLEEFRKLVVEDGEKIYEENKVG
ECEEEEECCCEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure
MNEQWIFLNGEFVPKDEAKVSVYDHGYLYGDGVFEGIRVYSGNVFRLREHLVRLYESAKS
CCCCEEEECCEECCCCCCEEEEEECCEEEECCHHCCEEEECCCHHHHHHHHHHHHHHHHH
ILLEIPYSLDEVTNIVVETIRQNKLSNGYIRLVVSRGAGNLGLDPDSCKKPNVVVIAEQL
EEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHH
SLFPQEYYEKGIPVVTVATRRNRPDVLSPQVKSLNYLNNILVRIEAKLAGVQEALMLNDQ
HCCHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEHHHHCHHHHEEECCC
GYVAEGSGDNVFIVKGNKLITPPSSAGALEGITRNAILEIGEKLGYDVREELFTRHDVYV
CEEEECCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEE
ADEVFLTGTAAEVIAVTTVDGRTIGLGQTGPHTNRLLEEFRKLVVEDGEKIYEENKVG
ECEEEEECCCEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]