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The map label for this gene is ilvB [H]

Identifier: 218232047

GI number: 218232047

Start: 1391469

End: 1393169

Strand: Direct

Name: ilvB [H]

Synonym: BCB4264_A1450

Alternate gene names: 218232047

Gene position: 1391469-1393169 (Clockwise)

Preceding gene: 218235632

Following gene: 218234465

Centisome position: 25.68

GC content: 39.45

Gene sequence:

>1701_bases
ATGTCTTCAAAAACGGAAGAGAAACTGGAAACTGGTGCACAGCTATTGTTAGAAGCGCTAGAAAAGGAAGGCGTAGAAGT
GATTTTCGGTTATCCGGGTGGTGCCGTTTTACCTCTCTATGATGCATTGTATGATTGTGAAATTCCGCATATTTTAACAA
GGCATGAGCAAGGGGCAATTCACGCTGCTGAAGGGTATGCCAGAATTACAGGGAATCCAGGGGTTGTAATTGCAACGAGT
GGACCAGGTGCTACAAATGTAATTACGGGTCTAGCTGATGCGATGATTGATTCATTGCCACTTGTAGTATTTACAGGCCA
AGTAGCAACAACGTTAATTGGGAGCGATGCTTTTCAAGAGGCAGATATTATGGGACTTACGATGCCAGTGACAAAACATA
ACTATCAAGTGCGAAAGGCCTCAGATTTACCTCGAATTATTAAAGAAGCATTTCATATTGCTAAAACAGGAAGACCAGGA
CCAGTCGTTATTGACCTTCCGAAAGATATGGTAGTCGAGAAAGGTGAGCGATGTAGCAATGTACAAATGGATTTACCAGG
ATATAACCCTAATTATGAACCGAATCTACTTCAAATAAACAAATTACTCAAAGTAATTGAAACCGCAAAAAAACCGTTAA
TTTTAGCTGGAGCGGGTATATTGCATGCGAAAGCTTCGAAAGAATTGACAAGTTTTGCTCGTAAATATGAAATCCCTGTT
GTGCATACATTACTTGGACTTGGTGGTTTTCCACCAGATGATGAACTATTTCTAGGGATGGGGGGAATGCACGGTTCTTA
TACAGCCAATATGGCATTATATGAATGCGACTTACTCATTAATATAGGCGCGAGATTTGATGATCGTCTTACTGGAAATT
TAGCTTTTTTTGCTAAAGGGGCGACTGTTGCACATATCGATATTGATCCAGCAGAAATCGGGAAAAATGTGCCAACAGAG
ATTCCTATTGTTGCAAGTGCTAAGCAGGCGTTAGAAGTATTGCTTCAATCAGAAGGAAAGAAAGAAAATCATCATGAATG
GCTCTTTTTATTAAAGAATAGAAAAGAAAAATATCCATTCTCTTATAAAAGAAATTCTGAAAGTATTAAACCTCAATATG
CGATTGATATGCTATATGAAATTACGAAGGGAGAAGCGATTGTAACAACAGACGTTGGGCAACACCAAATGTGGGCAGCG
CAATATTATCCACTGAAAAATCCAGATAAATGGGTAACTTCTGGTGGATTAGGAACGATGGGGTTCGGATTTCCAGCAGC
AATCGGTGCGCAAATTGCAAAGCCTGAAGAATTAGTTATTGCAATTGTTGGTGATGCTGGGTTTCAAATGACTCTGCAAG
AATTAAGTGTATTAAAAGAACACGCTTTACCCGTTAAAGTATTTATTTTAAATAATGAAGCTTTAGGAATGGTAAGACAA
TGGCAAGATGAATTTTACAATCAAAGATATTCACATTCTTTATTACCATGCCAACCAGATTTCGTCGCTCTTGCAAATGC
ATATGGCATAAAAGGTGTTCGTATTGACGATCCACTTCTTGCAAAGAAGCAAATCCAGCATGCGATCGAATTACAAGAGC
CAGTCGTAATTGATTGTCGTGTACTTCAATCAGAGAAGGTTATGCCGATGGTTGCGCCAGGAAAAGGTGTTCACCAAATG
GAGGGAGTGGAAAAAGGGTGA

Upstream 100 bases:

>100_bases
TGGTACCGCGAAATCTTTCGTCCCTTCAGCACATAGCTGGAGTGTGGACGTAGGATTTTTTTATTGTATAAAAGGTTGAA
AAAGGGGGCTTATAAGAACA

Downstream 100 bases:

>100_bases
AGAGAATTGTTACAGCGACAGTTCGAAATCAAAGTGGTGTGTTAAACCGAATTACAGGGGTTATGACACGAAGACATTTT
AATATTGAAAGTATTTCAGT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MSSKTEEKLETGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYARITGNPGVVIATS
GPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIAKTGRPG
PVVIDLPKDMVVEKGERCSNVQMDLPGYNPNYEPNLLQINKLLKVIETAKKPLILAGAGILHAKASKELTSFARKYEIPV
VHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAFFAKGATVAHIDIDPAEIGKNVPTE
IPIVASAKQALEVLLQSEGKKENHHEWLFLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAA
QYYPLKNPDKWVTSGGLGTMGFGFPAAIGAQIAKPEELVIAIVGDAGFQMTLQELSVLKEHALPVKVFILNNEALGMVRQ
WQDEFYNQRYSHSLLPCQPDFVALANAYGIKGVRIDDPLLAKKQIQHAIELQEPVVIDCRVLQSEKVMPMVAPGKGVHQM
EGVEKG

Sequences:

>Translated_566_residues
MSSKTEEKLETGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYARITGNPGVVIATS
GPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIAKTGRPG
PVVIDLPKDMVVEKGERCSNVQMDLPGYNPNYEPNLLQINKLLKVIETAKKPLILAGAGILHAKASKELTSFARKYEIPV
VHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAFFAKGATVAHIDIDPAEIGKNVPTE
IPIVASAKQALEVLLQSEGKKENHHEWLFLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAA
QYYPLKNPDKWVTSGGLGTMGFGFPAAIGAQIAKPEELVIAIVGDAGFQMTLQELSVLKEHALPVKVFILNNEALGMVRQ
WQDEFYNQRYSHSLLPCQPDFVALANAYGIKGVRIDDPLLAKKQIQHAIELQEPVVIDCRVLQSEKVMPMVAPGKGVHQM
EGVEKG
>Mature_565_residues
SSKTEEKLETGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYARITGNPGVVIATSG
PGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIAKTGRPGP
VVIDLPKDMVVEKGERCSNVQMDLPGYNPNYEPNLLQINKLLKVIETAKKPLILAGAGILHAKASKELTSFARKYEIPVV
HTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAFFAKGATVAHIDIDPAEIGKNVPTEI
PIVASAKQALEVLLQSEGKKENHHEWLFLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQ
YYPLKNPDKWVTSGGLGTMGFGFPAAIGAQIAKPEELVIAIVGDAGFQMTLQELSVLKEHALPVKVFILNNEALGMVRQW
QDEFYNQRYSHSLLPCQPDFVALANAYGIKGVRIDDPLLAKKQIQHAIELQEPVVIDCRVLQSEKVMPMVAPGKGVHQME
GVEKG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=577, Percent_Identity=24.263431542461, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI21361361, Length=513, Percent_Identity=24.3664717348928, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=46.6192170818505, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=40.8199643493761, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1786717, Length=517, Percent_Identity=35.2030947775629, Blast_Score=313, Evalue=3e-86,
Organism=Escherichia coli, GI1787096, Length=545, Percent_Identity=27.7064220183486, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1788716, Length=483, Percent_Identity=24.223602484472, Blast_Score=138, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=485, Percent_Identity=29.2783505154639, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=485, Percent_Identity=29.2783505154639, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=564, Percent_Identity=24.645390070922, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=573, Percent_Identity=45.3752181500873, Blast_Score=482, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6320816, Length=484, Percent_Identity=23.5537190082645, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=488, Percent_Identity=23.5655737704918, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922626, Length=483, Percent_Identity=26.7080745341615, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61884; Mature: 61753

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKTEEKLETGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAI
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHCCCCCCE
HAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQE
EECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCHHCC
ADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIAKTGRPGPVVIDLPKDMVVEKGERCSN
CCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHCCCCCCC
VQMDLPGYNPNYEPNLLQINKLLKVIETAKKPLILAGAGILHAKASKELTSFARKYEIPV
CEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCCCHH
VHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAFFAKG
HHHHHHCCCCCCCCCEEEEECCCCCCEEECEEEEEEEEEEEECCCCCCCCCCCEEEEECC
ATVAHIDIDPAEIGKNVPTEIPIVASAKQALEVLLQSEGKKENHHEWLFLLKNRKEKYPF
CEEEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
SYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQYYPLKNPDKWVTSGGLGTM
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCCEEECCCCCCC
GFGFPAAIGAQIAKPEELVIAIVGDAGFQMTLQELSVLKEHALPVKVFILNNEALGMVRQ
CCCCHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHH
WQDEFYNQRYSHSLLPCQPDFVALANAYGIKGVRIDDPLLAKKQIQHAIELQEPVVIDCR
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEE
VLQSEKVMPMVAPGKGVHQMEGVEKG
EECCCCCCEEECCCCCCHHHCCCCCC
>Mature Secondary Structure 
SSKTEEKLETGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAI
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHCCCCCCE
HAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQE
EECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCHHCC
ADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIAKTGRPGPVVIDLPKDMVVEKGERCSN
CCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHCCCCCCC
VQMDLPGYNPNYEPNLLQINKLLKVIETAKKPLILAGAGILHAKASKELTSFARKYEIPV
CEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCCCHH
VHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAFFAKG
HHHHHHCCCCCCCCCEEEEECCCCCCEEECEEEEEEEEEEEECCCCCCCCCCCEEEEECC
ATVAHIDIDPAEIGKNVPTEIPIVASAKQALEVLLQSEGKKENHHEWLFLLKNRKEKYPF
CEEEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
SYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQYYPLKNPDKWVTSGGLGTM
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCCEEECCCCCCC
GFGFPAAIGAQIAKPEELVIAIVGDAGFQMTLQELSVLKEHALPVKVFILNNEALGMVRQ
CCCCHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHH
WQDEFYNQRYSHSLLPCQPDFVALANAYGIKGVRIDDPLLAKKQIQHAIELQEPVVIDCR
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEE
VLQSEKVMPMVAPGKGVHQMEGVEKG
EECCCCCCEEECCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]