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The map label for this gene is cysW [H]

Identifier: 218235428

GI number: 218235428

Start: 1103825

End: 1104682

Strand: Direct

Name: cysW [H]

Synonym: BCB4264_A1127

Alternate gene names: 218235428

Gene position: 1103825-1104682 (Clockwise)

Preceding gene: 218232070

Following gene: 218231760

Centisome position: 20.37

GC content: 39.63

Gene sequence:

>858_bases
ATGGAACCAACAGTATTAGAAAAGAAAATAGTAAAAAGCGTATCAGCTAAAAAAGAATCTAAGCTTGTACCAGGCGTATT
AATTACGATTACAGTTTTATTTTTATCTCTCTTTCTATTACTGCCACTCATAACTATTTTTTTAAAAGCCTTTGAAAGAG
GGATGGATGTATACTTTGCCGCTATCACTGATCAAGAAGCATTTTCAGCAATTAGGTTAACGCTTCTCGTTGCGTTAATC
GTTGTACCGCTGAATACGGTATTCGGAATCGCAGCGGCATGGCTTATTACAAAGTTTCAGTTTAAAGGAAAACAAGTATT
ACTCTCTCTTATTGAACTGCCGTTTGCTGTGTCACCAGTTATCGCAGGATTAGTATTCGTTTTACTTTTCACACCGAGGG
GTGCTCTTGGCGAATGGTTACTTGAACATGGAGTGAAACTCATTTTCTCTGTACCAGGCATTATTATCGCAACAATCTTT
GTAACCTTCCCGTTCGTTGCACGTGAATTAATTCCGATTATGCAGGCGCAAGGGAAAAGTGAAGAAGAGGCAGCTTTATC
ACTTGGCGCAAGTGGCTGGAAAATGTTTTGGCGCGTTACACTTCCAAATATCAAATGGGGTCTTTTGTACGGCATGATTC
TATGTAATGCCCGTGCTATCGGTGAGTTTGGAGCCGTTTCTGTCGTATCAGGTCACGTGCGTGGCATTACGAACACGATG
CCGCTCCATATTGAAATTTTGTACAATGAATATCAATTTTCAGCAGCATTTGCAGTAGCGACGTTAATGTCATTAATTGC
AGTGTTTACACTCGTTATAAAAAACTGGATTGAATGGCGAATGGAAAAACGACAATAA

Upstream 100 bases:

>100_bases
CCGTGATGCTTATTATTTCTCTCCTTTTCCTTCTTCTTATTAATATGATTCAAAGCTGGAGTAGAAGGCATGAACTAAAA
AGTGAATAGGGGGAGAGAAA

Downstream 100 bases:

>100_bases
GAGGGGGATTACGGTGAGTATTCAAATTCAAGGTGTATCAAAGCAATATGGTACATTTCAAGCACTAACAGACATTCATT
TGGATATTCCAAAAGGCGAA

Product: sulfate ABC transporter, permease protein CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MEPTVLEKKIVKSVSAKKESKLVPGVLITITVLFLSLFLLLPLITIFLKAFERGMDVYFAAITDQEAFSAIRLTLLVALI
VVPLNTVFGIAAAWLITKFQFKGKQVLLSLIELPFAVSPVIAGLVFVLLFTPRGALGEWLLEHGVKLIFSVPGIIIATIF
VTFPFVARELIPIMQAQGKSEEEAALSLGASGWKMFWRVTLPNIKWGLLYGMILCNARAIGEFGAVSVVSGHVRGITNTM
PLHIEILYNEYQFSAAFAVATLMSLIAVFTLVIKNWIEWRMEKRQ

Sequences:

>Translated_285_residues
MEPTVLEKKIVKSVSAKKESKLVPGVLITITVLFLSLFLLLPLITIFLKAFERGMDVYFAAITDQEAFSAIRLTLLVALI
VVPLNTVFGIAAAWLITKFQFKGKQVLLSLIELPFAVSPVIAGLVFVLLFTPRGALGEWLLEHGVKLIFSVPGIIIATIF
VTFPFVARELIPIMQAQGKSEEEAALSLGASGWKMFWRVTLPNIKWGLLYGMILCNARAIGEFGAVSVVSGHVRGITNTM
PLHIEILYNEYQFSAAFAVATLMSLIAVFTLVIKNWIEWRMEKRQ
>Mature_285_residues
MEPTVLEKKIVKSVSAKKESKLVPGVLITITVLFLSLFLLLPLITIFLKAFERGMDVYFAAITDQEAFSAIRLTLLVALI
VVPLNTVFGIAAAWLITKFQFKGKQVLLSLIELPFAVSPVIAGLVFVLLFTPRGALGEWLLEHGVKLIFSVPGIIIATIF
VTFPFVARELIPIMQAQGKSEEEAALSLGASGWKMFWRVTLPNIKWGLLYGMILCNARAIGEFGAVSVVSGHVRGITNTM
PLHIEILYNEYQFSAAFAVATLMSLIAVFTLVIKNWIEWRMEKRQ

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=259, Percent_Identity=53.2818532818533, Blast_Score=290, Evalue=6e-80,
Organism=Escherichia coli, GI1788764, Length=227, Percent_Identity=34.8017621145374, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1786980, Length=218, Percent_Identity=31.651376146789, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787369, Length=220, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31598; Mature: 31598

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPTVLEKKIVKSVSAKKESKLVPGVLITITVLFLSLFLLLPLITIFLKAFERGMDVYFA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
AITDQEAFSAIRLTLLVALIVVPLNTVFGIAAAWLITKFQFKGKQVLLSLIELPFAVSPV
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHH
IAGLVFVLLFTPRGALGEWLLEHGVKLIFSVPGIIIATIFVTFPFVARELIPIMQAQGKS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEEAALSLGASGWKMFWRVTLPNIKWGLLYGMILCNARAIGEFGAVSVVSGHVRGITNTM
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
PLHIEILYNEYQFSAAFAVATLMSLIAVFTLVIKNWIEWRMEKRQ
CEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEPTVLEKKIVKSVSAKKESKLVPGVLITITVLFLSLFLLLPLITIFLKAFERGMDVYFA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
AITDQEAFSAIRLTLLVALIVVPLNTVFGIAAAWLITKFQFKGKQVLLSLIELPFAVSPV
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHH
IAGLVFVLLFTPRGALGEWLLEHGVKLIFSVPGIIIATIFVTFPFVARELIPIMQAQGKS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEEAALSLGASGWKMFWRVTLPNIKWGLLYGMILCNARAIGEFGAVSVVSGHVRGITNTM
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
PLHIEILYNEYQFSAAFAVATLMSLIAVFTLVIKNWIEWRMEKRQ
CEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]