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The map label for this gene is tilS [H]

Identifier: 218235253

GI number: 218235253

Start: 67432

End: 68862

Strand: Direct

Name: tilS [H]

Synonym: BCB4264_A0070

Alternate gene names: 218235253

Gene position: 67432-68862 (Clockwise)

Preceding gene: 218231970

Following gene: 218236151

Centisome position: 1.24

GC content: 35.01

Gene sequence:

>1431_bases
TTGAAAGATACATTTGTTGAAAAAGTAGATGACTTTGTAAAGCAGCATGATGTATTAAAGAATGATTCAACAATTGTTGT
GGGAGTTTCTGGTGGTCCTGACTCGTTAGCGCTTTTATATTATTTATTAGAAAAAAGAGCGGAAAAACAGTTGGAAATTG
TAGTTGCTCATGTGGATCATATGTTTAGAGGTGATGAATCTTATGAGGATTTACAGTTTGTGCAGGGCCTTTGTGAAGAA
CTGGGAATTATTTGTGAAACGATAAGGATTAATGTATCACAATATCAACAGCAATACGGAATGAATGCACAGGTTGCTGC
GAGAGAATGCAGATATGCATTTTTGGAAAGAATAATGAAGAAATATGATGCGAGATATGTTGCTCTTGGACATCATGGAG
ATGATCAAGTCGAGACAATTTTAATGCGTCTTGTAAGAGGGAGTACTCCGAAAGGATATGCAGGAATTGCAGTGAAGCGT
CCTTTTCATAATGGATATTTAATTAGGCCGTTACTTGGCGTAACGAAGGAAGAAATTGTTGATTACTGTAATAAGCTAAA
TTTAATGCCACGTATAGATCCGAGTAATAAAAAGGAAGTATATACAAGGAATCGATTACGTAAATATGTCCTTCCTCATT
TGAAAGAAGAAAATCCACAAATGCATGAGAAATTCCAAAAATTTAGCGTGCAGATGCAAGAGGATGAGGCTTATTTGCAG
GAATTAGCTTTTGAGAAAATGAATAAAGTAATTACAAAAAAAAGTGATAAACAAATTAGCTTATCAATTCCTGCCTTTGA
ATCCATGTCTATGCCTTTACAAAGAAGAGGGATTCAACTAATATTAAACTATCTTTATGAATATAAGATTCCATCTTCTC
TTTCTTCTATACATATTGACAAGTTGATTGAGTTTTTTAAGCGGACACAACCTTCAGGTTCACTTGATTTTCCGGGTGAT
TTGAAAATTGTTCGTTCATACGAGGAGTGTAGCTTTAGATTTAAACAAGAAATTGTCTCTCCTTTTTCACAAGATTTATT
AGTACCCGGGACCATTACGCTATTGAACGGGGATGAATTTGTAACAGAGGTGAGCGAAGATATACCAAGTAACATGAATG
AAACAGTATTTGTTGCTAAGTATAATGATATATCATATCCACTTCGTATTCGTTCTAGAGAAAATGGAGATCGCATGTCA
ATACAAGGTATGAATGGTACGAAAAAGATAAAAGCTATTTTTATCGAAGCGAAAGTACCGAAAGAAAAAAGAGAAGAATG
GCCGGTCGTTTGTGATGCAAGTGGGACTATTATTTGGGTACCCTTGTTGAAACGATCTGCATTTGCAATTTCGAAAGAGA
TGGCAAAGAAGGATAAATATATGATTATTCACTACAAAAGCAAGGAGTCTTCCGGGAGGATAATGAAATGA

Upstream 100 bases:

>100_bases
TTTGTATATCTTTTTTTTGCACAATGTTTGTAAAAGTTGTACCATTGTAGACAAGGTGACTAAGGTTGCTGTTCTTGTTT
AATGAAAGGTGATTGCGAAG

Downstream 100 bases:

>100_bases
TGAATCAAGATATCGAAAAAGTATTAATTTCTGAAGAGCAAATACAAGAAAAGGTGCGCGAACTAGGTGCAATTATTGCA
GAGGATTATAAAAACACAGT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKDTFVEKVDDFVKQHDVLKNDSTIVVGVSGGPDSLALLYYLLEKRAEKQLEIVVAHVDHMFRGDESYEDLQFVQGLCEE
LGIICETIRINVSQYQQQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVDYCNKLNLMPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQMHEKFQKFSVQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKLIEFFKRTQPSGSLDFPGD
LKIVRSYEECSFRFKQEIVSPFSQDLLVPGTITLLNGDEFVTEVSEDIPSNMNETVFVAKYNDISYPLRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPKEKREEWPVVCDASGTIIWVPLLKRSAFAISKEMAKKDKYMIIHYKSKESSGRIMK

Sequences:

>Translated_476_residues
MKDTFVEKVDDFVKQHDVLKNDSTIVVGVSGGPDSLALLYYLLEKRAEKQLEIVVAHVDHMFRGDESYEDLQFVQGLCEE
LGIICETIRINVSQYQQQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVDYCNKLNLMPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQMHEKFQKFSVQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKLIEFFKRTQPSGSLDFPGD
LKIVRSYEECSFRFKQEIVSPFSQDLLVPGTITLLNGDEFVTEVSEDIPSNMNETVFVAKYNDISYPLRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPKEKREEWPVVCDASGTIIWVPLLKRSAFAISKEMAKKDKYMIIHYKSKESSGRIMK
>Mature_476_residues
MKDTFVEKVDDFVKQHDVLKNDSTIVVGVSGGPDSLALLYYLLEKRAEKQLEIVVAHVDHMFRGDESYEDLQFVQGLCEE
LGIICETIRINVSQYQQQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVDYCNKLNLMPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQMHEKFQKFSVQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKLIEFFKRTQPSGSLDFPGD
LKIVRSYEECSFRFKQEIVSPFSQDLLVPGTITLLNGDEFVTEVSEDIPSNMNETVFVAKYNDISYPLRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPKEKREEWPVVCDASGTIIWVPLLKRSAFAISKEMAKKDKYMIIHYKSKESSGRIMK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=220, Percent_Identity=30, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 55025; Mature: 55025

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTFVEKVDDFVKQHDVLKNDSTIVVGVSGGPDSLALLYYLLEKRAEKQLEIVVAHVDH
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFRGDESYEDLQFVQGLCEELGIICETIRINVSQYQQQYGMNAQVAARECRYAFLERIMK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKRPFHNGYLIRPLLGVTKEEIV
HHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHCCCHHHHH
DYCNKLNLMPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQMHEKFQKFSVQMQEDEAYLQ
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCEEHHHHHHHH
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHID
HHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KLIEFFKRTQPSGSLDFPGDLKIVRSYEECSFRFKQEIVSPFSQDLLVPGTITLLNGDEF
HHHHHHHHCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCHHHCCCCCCCEEEEECCHHH
VTEVSEDIPSNMNETVFVAKYNDISYPLRIRSRENGDRMSIQGMNGTKKIKAIFIEAKVP
HHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCC
KEKREEWPVVCDASGTIIWVPLLKRSAFAISKEMAKKDKYMIIHYKSKESSGRIMK
HHHHCCCCEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKDTFVEKVDDFVKQHDVLKNDSTIVVGVSGGPDSLALLYYLLEKRAEKQLEIVVAHVDH
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFRGDESYEDLQFVQGLCEELGIICETIRINVSQYQQQYGMNAQVAARECRYAFLERIMK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKRPFHNGYLIRPLLGVTKEEIV
HHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHCCCHHHHH
DYCNKLNLMPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQMHEKFQKFSVQMQEDEAYLQ
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCEEHHHHHHHH
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHID
HHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KLIEFFKRTQPSGSLDFPGDLKIVRSYEECSFRFKQEIVSPFSQDLLVPGTITLLNGDEF
HHHHHHHHCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCHHHCCCCCCCEEEEECCHHH
VTEVSEDIPSNMNETVFVAKYNDISYPLRIRSRENGDRMSIQGMNGTKKIKAIFIEAKVP
HHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCC
KEKREEWPVVCDASGTIIWVPLLKRSAFAISKEMAKKDKYMIIHYKSKESSGRIMK
HHHHCCCCEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]