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The map label for this gene is gltX [H]

Identifier: 218234593

GI number: 218234593

Start: 102928

End: 104403

Strand: Direct

Name: gltX [H]

Synonym: BCB4264_A0108

Alternate gene names: 218234593

Gene position: 102928-104403 (Clockwise)

Preceding gene: 218233135

Following gene: 218236099

Centisome position: 1.9

GC content: 36.99

Gene sequence:

>1476_bases
ATGGAAGGTGTACCAATTATGGAAAAGCAAGTGAGAGTGCGCTACGCGCCAAGTCCAACAGGACACTTACATATCGGAAA
TGCGCGTACGGCATTATTTAATTATTTATTTGCTCGTCATTTAGATGGCAAATTTATTATTCGTATTGAAGATACTGATG
TAAAACGTAACGTGGCTGGTGGAGAAGAAAGCCAATTAAAATACTTGAAATGGCTCGGTATGGACTGGGATGAAGGTGTT
GATGTTGGTGGTGAATTTGGACCATATCGCCAAACAGAGCGTTTAGATATTTATAAAAAATTATATGAAGATTTATTAGA
GCGTGGTCTAGCTTATAAATGTTATATGACAGAAGAAGAGCTAGAAGCAGAGCGTGAAGGACAAATCGCTCGCGGTGAAA
CACCTCGTTACGCAGGTAACCACCGTGAATTAACTGAAGAACAAGTGAAAGAATTTGAAGCTGAGGGACGTATTCCAAGT
ATTCGTTTCCGTGTACCAGCTGATCGTGACTACACATTCAAAGATATTGTAAAAGATGAAGTTGCGTTCCATTCAAATGA
TTTCGGTGATTTTGTTATTGTGAAAAAGGATGGAATTCCAACTTATAACTTTGCAGTAGCAGTAGATGATCATCTAATGG
AAATTACACATGTACTTCGTGGTGATGATCATATTTCAAATACACCGAAACAAATGATGATTTATGAAGCTTTCGGCTGG
GATATTCCACAATTCGGTCATATGACTCTAATTGTTAATGAAAGTCGTAAAAAGTTAAGTAAGCGTGATGAATCTATTAT
TCAATTTATTGAGCAATATAAAGAGCTTGGGTATCTTCCAGAAGCAATCTTTAACTTTATTGCACTACTAGGTTGGTCTC
CGGTAGGAGAAGAAGAAATCTTCTCTAAAGATGAGTTTATCAAAATGTTCGATGCAGCTCGTTTATCAAAATCACCTGCA
TTATTTGATTCTCAAAAACTAAAATGGATGAACAACCAGTATATGAAAAAGCAAGATTTAGATACGGTTGTAGAGTTAAG
CTTACCGCATTTAGTGAAAGCTGGACGTGTGGGAGAAACTTTAAGCGAACAAGACCAAGCTTGGATTCGTGATGTAATTG
CTTTATACCATGAACAAATGAGTTTTGGAGCTGAAATTGTAGAGCTTTCTGAAATGTTCTTTAAAGATCATGTTGATTAT
GAAGAAGAAGGACAAGAAGTACTAAATGGTGAACAAGTACCTGAAGTACTTCGTGCATTTGCTGGTCAAATAGAAGCTCT
AGAAGCAATGGAGCCAGCAGCAATTAAGGCAGCGATTAAAGCAGTTCAAAAGGAAACAGGACATAAAGGTAAAAATTTAT
TTATGCCAATCCGTGTTGCAACTACAGGACAAACGCATGGTCCAGAGCTTCCGAATGCTATTGCACTTCTTGGAAAAGAA
AAAGTTTTAAATCGTCTTCAAAAAGTAATTGGTTAA

Upstream 100 bases:

>100_bases
AAGCGGTTGTTTTATTACAGAAAAAATAATGGTTTTTAATTCGTAAGATGGATAATCACATTTTTTTGGTGTACAATTAT
AGAATGTGTTGACATTAAGA

Downstream 100 bases:

>100_bases
CATTTTCTAGGTTTAGAAATATAATAAGTATACCTAAAACCTAATAAGGAAAAGCGACGAGAAGGAGAAGTAGAAAATCA
GGCTTTTTACAGAGAGAACC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHLDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMDWDEGV
DVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRELTEEQVKEFEAEGRIPS
IRFRVPADRDYTFKDIVKDEVAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGW
DIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEIFSKDEFIKMFDAARLSKSPA
LFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRVGETLSEQDQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDY
EEEGQEVLNGEQVPEVLRAFAGQIEALEAMEPAAIKAAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKE
KVLNRLQKVIG

Sequences:

>Translated_491_residues
MEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHLDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMDWDEGV
DVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRELTEEQVKEFEAEGRIPS
IRFRVPADRDYTFKDIVKDEVAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGW
DIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEIFSKDEFIKMFDAARLSKSPA
LFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRVGETLSEQDQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDY
EEEGQEVLNGEQVPEVLRAFAGQIEALEAMEPAAIKAAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKE
KVLNRLQKVIG
>Mature_491_residues
MEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHLDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMDWDEGV
DVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRELTEEQVKEFEAEGRIPS
IRFRVPADRDYTFKDIVKDEVAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGW
DIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEIFSKDEFIKMFDAARLSKSPA
LFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRVGETLSEQDQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDY
EEEGQEVLNGEQVPEVLRAFAGQIEALEAMEPAAIKAAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKE
KVLNRLQKVIG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=503, Percent_Identity=33.3996023856859, Blast_Score=255, Evalue=8e-68,
Organism=Homo sapiens, GI4826960, Length=324, Percent_Identity=24.3827160493827, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI62241042, Length=256, Percent_Identity=24.609375, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788743, Length=481, Percent_Identity=35.3430353430353, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI226510925, Length=255, Percent_Identity=33.7254901960784, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71993394, Length=500, Percent_Identity=32.6, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324540, Length=505, Percent_Identity=33.8613861386139, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6324742, Length=323, Percent_Identity=25.6965944272446, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=220, Percent_Identity=27.7272727272727, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=34.1463414634146, Blast_Score=278, Evalue=9e-75,
Organism=Drosophila melanogaster, GI17864488, Length=310, Percent_Identity=25.1612903225806, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56259; Mature: 56259

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHLDGKFIIRIEDTDVKRNVAG
CCCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCHHCCCC
GEESQLKYLKWLGMDWDEGVDVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEE
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHH
LEAEREGQIARGETPRYAGNHRELTEEQVKEFEAEGRIPSIRFRVPADRDYTFKDIVKDE
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHH
VAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGW
HHHCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCHHEEEEHHHCC
DIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEI
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHH
FSKDEFIKMFDAARLSKSPALFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRVGET
HCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LSEQDQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDYEEEGQEVLNGEQVPEVLRAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHH
AGQIEALEAMEPAAIKAAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKE
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHH
KVLNRLQKVIG
HHHHHHHHHHC
>Mature Secondary Structure
MEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHLDGKFIIRIEDTDVKRNVAG
CCCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCHHCCCC
GEESQLKYLKWLGMDWDEGVDVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEE
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHH
LEAEREGQIARGETPRYAGNHRELTEEQVKEFEAEGRIPSIRFRVPADRDYTFKDIVKDE
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHH
VAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGW
HHHCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCHHEEEEHHHCC
DIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEI
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHH
FSKDEFIKMFDAARLSKSPALFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRVGET
HCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LSEQDQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDYEEEGQEVLNGEQVPEVLRAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHH
AGQIEALEAMEPAAIKAAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKE
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHH
KVLNRLQKVIG
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA