| Definition | Bacillus cereus B4264, complete genome. |
|---|---|
| Accession | NC_011725 |
| Length | 5,419,036 |
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The map label for this gene is pulA [H]
Identifier: 218234254
GI number: 218234254
Start: 2644081
End: 2646639
Strand: Reverse
Name: pulA [H]
Synonym: BCB4264_A2741
Alternate gene names: 218234254
Gene position: 2646639-2644081 (Counterclockwise)
Preceding gene: 218234167
Following gene: 218232695
Centisome position: 48.84
GC content: 33.76
Gene sequence:
>2559_bases GTGCAAATTATAAAAAGATTAATTAACAAAACAGTTTTATTACTTACTTTAATCGTTATGTTATCATCTGTTTTCTCCTT TCAGAGTGTGAAGGCAGTTTCAAATTCGAAAACAACTGAAATTATCATTCATTACAAAGAAAAGCTTGGAAATACAAAAG ACTGGAATCTTTGGTTATGGGGAGAAAATGCAAATGGTACATCTTATGAATTTACTGGTGAAGATGAATTTGGAAAATAC GCTAAGATAAAAATAGACGGTGACTATAATCGTGTAGGATTTATAGTTCGAACAAATGAATGGGAAAAAGATGGTGGGGA TCGTTGGATTGAAAATATAAAGGACGGTCGTGCTGAAGTATGGATTCTTTCGGGGGATGAAAAAGTATATAATTCTAAGC CATCATCGGATCTCTCAATTCAAAAGGCAACTATTGATAGTTTCCATGAAATTACTGTAACGACTAATGTCCCGTTTCAT ATTAAGGAAAAGAAAATTGAAATTGAAGGCATTAAAATTAAGAATATTACTCCTTATGATATAAACAGTGGAGATATTAC TAATAAGGTCAAAATAATTACGGAACAGAAAATTGATTTAAAACAAACATACAAAGTTAAAATAGAAAACTTAGCTGATA CGAATACTGAAATTGGTAAAGTCATTCGTAGCGAAGAATTTGATAATTTATTTTATTATGGTGGTAACGATTTAGGAAAT ACATATACTCCGCAACATACAAAGTTTCGTTTATGGGCTCCTACTGCAAGCGAAGCGAAGTTAGTTACATATAAAAACTG GAACGATAAAATAGGTGCTGAAATAAACATGCAACAAGGTGAAAAGGGAACTTGGACAGCAGAACTAAAGGGGAACCAAA AAGGGCTCTTTTATACGTATAAGGTCAAAATTAGTGATAAGTGGACTGAAGCGGTTGATCCATATGTGCGTGCTGCTTCT GTAAATGGGGATAAAGGTGCAGTCGTTAATTTAGAAGAAACAAATCCGAAAAAATGGAAGGCAAACAAAAAGCCAAAATT AAAAAATCCGGAAGATGCTATTATTTATGAGTTACATGTACGCGACTTTTCCATTCAACCTGAAAGTGGTATTAAACAGA AAGGGAAGTATTTAGGTGTAACAGAAAAAGGAACAAAAGGGCCAGAAGGTGTAAAAACAGGACTTGATCATATAAAGGAT CTTGGTGTTACTCACGTTCAGTTTTTACCGATATTTGATTATGCTTCTGTAAATGAAGAGACTTTAAATGAACCGCAATA TAACTGGGGATATGATCCTAAAAATTTCAATGTTCCAGAAGGCTCTTATTCTACAAATCCTTATGAGCCGACTGTGCGAA TAACTGAATTAAAGCAAATGGTTCAAACACTACATGATAATAATCTTCGAGTGGTGATGGATGTAGTATATAACCATATG TATAACGCTGCTGAATCTAATTTCCATAAGTTAGTTCCAGGTTATTATTATCGTTACAATGAAGATGGAACATTTGCAAA TGGAACTGGAGTAGGAAATGATACTGCTTCAGAAAGAAAAATGATGAGGAAATTTATGATAGATTCCGTCACATATTGGG CAAAAGAATACAATTTAGATGGATTCCGTTTTGATTTAATGGGTATTCATGATTATGAAACGATGAATGAAATACGTAAA GCTGTTAATCAAATTGACCCTTCTATTATACTACACGGAGAAGGATGGGATTTAAATACACCTCTTGCAGCTGAGCTAAA AGCAAATCAAAAAAATGCAGAGAAAATGAAAAGGATTGCTCATTTTAACGATAATATACGTGATGGGTTAAAAGGTAGTG TATTTGAGGAAAAAGAAAATGGTTTTATAAATGGAAAGGAAAACATGGAAGATCGTATTAAAAAAGGTATTACGGCAGGT ATTGACTACGATACAAATTCGTCTACCTATCAAGATCCAGAGCAGGTGCTAACTTATGTGGAAGCACATGACAATCATAC ATTATGGGATAAACTTGAGTTAACTAATCCAGGTGACAGCGATGAAGTGCGAACACAAATGCACAAATTGTCTTCTTCGA TTTTATTAACATCACAAGGAATTCCATTTTTACATGCAGGGCAAGAGTTTATGCGGACGAAATACGGTGATCATAATAGT TACAAATCTCCAGACTCAATTAACCAGATGGATTGGCTGCGCCGTGCAACGTTTAATAATGAAGTAGATTATATGAAGGG TTTAATAGAATTACGCAAAAAGTACTCAGCTTTTCGAATGACATCTGCAGAGCAAATAAAAACACACGTATCATTTATTG ATGCTCCAAAAAATACTGTAGCTTATATAATAGAGGGGAATAAACATGAATACTTTACATTGGCTCACAATGCAAATAGA GAAGCTGTTGAAATAACACTTCCATCAAAAGGGCCTTGGAAAGTACTAGTAGATGGCAAGCAGGCGGGAAGTAAACCACT TTATGTTGTCCATGACAATAAAATTAAAGTTCCTGCATTAAGTTCCATTGTTTTAAAATCAGAAAAACCAATTAAATAA
Upstream 100 bases:
>100_bases ACGGAGGTGAAGTAAAAGAGATTTCTAGATTAATTTAAGCAGTGTTATGTAGTGAAAGCATAATAAATCGTTAGTAATAT TTGAAGAAAGGGTGTATGTG
Downstream 100 bases:
>100_bases AAGCAAAGAGTGTGGAAATAAATACTGCATTGAGCAAAGTTAATAAGGATTACAAATGAAAAAAGCGAAGTAAAAACAAC CTTTACTTCGCTTTTAAAAT
Product: putative pullulanase
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 852; Mature: 852
Protein sequence:
>852_residues MQIIKRLINKTVLLLTLIVMLSSVFSFQSVKAVSNSKTTEIIIHYKEKLGNTKDWNLWLWGENANGTSYEFTGEDEFGKY AKIKIDGDYNRVGFIVRTNEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFH IKEKKIEIEGIKIKNITPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDNLFYYGGNDLGN TYTPQHTKFRLWAPTASEAKLVTYKNWNDKIGAEINMQQGEKGTWTAELKGNQKGLFYTYKVKISDKWTEAVDPYVRAAS VNGDKGAVVNLEETNPKKWKANKKPKLKNPEDAIIYELHVRDFSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHIKD LGVTHVQFLPIFDYASVNEETLNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMVQTLHDNNLRVVMDVVYNHM YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRK AVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKRIAHFNDNIRDGLKGSVFEEKENGFINGKENMEDRIKKGITAG IDYDTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPGDSDEVRTQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYSAFRMTSAEQIKTHVSFIDAPKNTVAYIIEGNKHEYFTLAHNANR EAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSIVLKSEKPIK
Sequences:
>Translated_852_residues MQIIKRLINKTVLLLTLIVMLSSVFSFQSVKAVSNSKTTEIIIHYKEKLGNTKDWNLWLWGENANGTSYEFTGEDEFGKY AKIKIDGDYNRVGFIVRTNEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFH IKEKKIEIEGIKIKNITPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDNLFYYGGNDLGN TYTPQHTKFRLWAPTASEAKLVTYKNWNDKIGAEINMQQGEKGTWTAELKGNQKGLFYTYKVKISDKWTEAVDPYVRAAS VNGDKGAVVNLEETNPKKWKANKKPKLKNPEDAIIYELHVRDFSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHIKD LGVTHVQFLPIFDYASVNEETLNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMVQTLHDNNLRVVMDVVYNHM YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRK AVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKRIAHFNDNIRDGLKGSVFEEKENGFINGKENMEDRIKKGITAG IDYDTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPGDSDEVRTQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYSAFRMTSAEQIKTHVSFIDAPKNTVAYIIEGNKHEYFTLAHNANR EAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSIVLKSEKPIK >Mature_852_residues MQIIKRLINKTVLLLTLIVMLSSVFSFQSVKAVSNSKTTEIIIHYKEKLGNTKDWNLWLWGENANGTSYEFTGEDEFGKY AKIKIDGDYNRVGFIVRTNEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFH IKEKKIEIEGIKIKNITPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDNLFYYGGNDLGN TYTPQHTKFRLWAPTASEAKLVTYKNWNDKIGAEINMQQGEKGTWTAELKGNQKGLFYTYKVKISDKWTEAVDPYVRAAS VNGDKGAVVNLEETNPKKWKANKKPKLKNPEDAIIYELHVRDFSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHIKD LGVTHVQFLPIFDYASVNEETLNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMVQTLHDNNLRVVMDVVYNHM YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRK AVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKRIAHFNDNIRDGLKGSVFEEKENGFINGKENMEDRIKKGITAG IDYDTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPGDSDEVRTQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYSAFRMTSAEQIKTHVSFIDAPKNTVAYIIEGNKHEYFTLAHNANR EAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSIVLKSEKPIK
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=669, Percent_Identity=20.9267563527653, Blast_Score=110, Evalue=4e-25,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR014756 - InterPro: IPR005323 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 97381; Mature: 97381
Theoretical pI: Translated: 7.04; Mature: 7.04
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 2.1 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQIIKRLINKTVLLLTLIVMLSSVFSFQSVKAVSNSKTTEIIIHYKEKLGNTKDWNLWLW CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCEEEEE GENANGTSYEFTGEDEFGKYAKIKIDGDYNRVGFIVRTNEWEKDGGDRWIENIKDGRAEV ECCCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEE WILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFHIKEKKIEIEGIKIKNITPYD EEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCEEEECCEEEEEEEEECCCCCCC INSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDNLFYYGGNDLGN CCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCC TYTPQHTKFRLWAPTASEAKLVTYKNWNDKIGAEINMQQGEKGTWTAELKGNQKGLFYTY CCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCCEEEEECCCCCCEEEEE KVKISDKWTEAVDPYVRAASVNGDKGAVVNLEETNPKKWKANKKPKLKNPEDAIIYELHV EEEECCCHHHHHCHHHEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEE RDFSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHIKDLGVTHVQFLPIFDYASVNEE EEEECCCCCCHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH TLNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMVQTLHDNNLRVVMDVVYNHM HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHH YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLD HHHHHCCHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC GFRFDLMGIHDYETMNEIRKAVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKRIA CEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEECCCCHHHHHHHHHH HFNDNIRDGLKGSVFEEKENGFINGKENMEDRIKKGITAGIDYDTNSSTYQDPEQVLTYV HCCCHHHCCCCCCCEECCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHH EAHDNHTLWDKLELTNPGDSDEVRTQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEECCHHHHHHHCCCCCC YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYSAFRMTSAEQIKTHVSFIDAPKNTV CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCEE AYIIEGNKHEYFTLAHNANREAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPAL EEEEECCCCEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEECCEECCCCH SSIVLKSEKPIK HHEEECCCCCCC >Mature Secondary Structure MQIIKRLINKTVLLLTLIVMLSSVFSFQSVKAVSNSKTTEIIIHYKEKLGNTKDWNLWLW CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCEEEEE GENANGTSYEFTGEDEFGKYAKIKIDGDYNRVGFIVRTNEWEKDGGDRWIENIKDGRAEV ECCCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEE WILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFHIKEKKIEIEGIKIKNITPYD EEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCEEEECCEEEEEEEEECCCCCCC INSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDNLFYYGGNDLGN CCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCC TYTPQHTKFRLWAPTASEAKLVTYKNWNDKIGAEINMQQGEKGTWTAELKGNQKGLFYTY CCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCCEEEEECCCCCCEEEEE KVKISDKWTEAVDPYVRAASVNGDKGAVVNLEETNPKKWKANKKPKLKNPEDAIIYELHV EEEECCCHHHHHCHHHEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEE RDFSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHIKDLGVTHVQFLPIFDYASVNEE EEEECCCCCCHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH TLNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMVQTLHDNNLRVVMDVVYNHM HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHH YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLD HHHHHCCHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC GFRFDLMGIHDYETMNEIRKAVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKRIA CEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEECCCCHHHHHHHHHH HFNDNIRDGLKGSVFEEKENGFINGKENMEDRIKKGITAGIDYDTNSSTYQDPEQVLTYV HCCCHHHCCCCCCCEECCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHH EAHDNHTLWDKLELTNPGDSDEVRTQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEECCHHHHHHHCCCCCC YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYSAFRMTSAEQIKTHVSFIDAPKNTV CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCEE AYIIEGNKHEYFTLAHNANREAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPAL EEEEECCCCEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEECCEECCCCH SSIVLKSEKPIK HHEEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9453151; 10360571 [H]