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The map label for this gene is rnjB [H]

Identifier: 218234241

GI number: 218234241

Start: 3813985

End: 3815655

Strand: Reverse

Name: rnjB [H]

Synonym: BCB4264_A3896

Alternate gene names: 218234241

Gene position: 3815655-3813985 (Counterclockwise)

Preceding gene: 218231209

Following gene: 218234620

Centisome position: 70.41

GC content: 36.68

Gene sequence:

>1671_bases
ATGAAGAGAAAAGAGAATGAGTCTGTTAAAGTATTTGCTCTTGGTGGAGTAGGTGAAATCGGGAAAAACATGTACTGTGT
TGAAATTGATTCTGAAATCTTTATCGTAGATGCAGGGTTAATGTTCCCGGGAGATGAAATGTTTGGAATTGATATCGTTA
TTCCTGATATTACATATTTAGTGGAAAATCAAGAGCGAGTAAAAGGTTTATTTATTACTCACGGTCATGAAGATCATATT
GGTGGAATTGTGTATGTGCTTCGTAAATTATCTATTCCAGTCTATGCAACGAAATTAACAGTAGGACTTATACAAGAAAA
ACTTGGTGAAGCGGGCATGTTAGGCCGTGTAGACCTAAAAACAATCGATTCAAATTCAACAGTAGAATTTAACACTACAA
CGGTGTCATTCTTCGGAACGACACATAGTATTCCGGATTCTGTTGGCGTTTGTTTCCATACATCAAAAGGTGCAGTCGTA
TATACGGGAGACTTTAAATTCGATCAAACTCCAATTGGAAATAGTGGAGCAGACCTTGGGAAAATGGCGCAAATCGGAAA
TGAAGGCGTACTTTGCTTATTATCTGATAGTACAAACGCAGAGCGCCCAGGCTATACAGGTTCAGAAAAAGAAGTTGGTG
TAGAAATCTCTAAAGTGTTCTACGGTGCAGAAGGTCGTATTATCGTTGCTTCATTTGCATCGAATGTACATCGTATTCAA
CAAGTATTTGATGCAGCTGCTGAAACAGGGCGAAAAGTAGCGGTAGTAGGACGTAGTATGGTGAAAGTAGTAGACATTGC
AAGACGTCTTGGTTATTTAAATGTTCCAGAAGGTATGGTTATTTCATTACAAGAAGTAGACAACTTCCCAGAGAAAAAGG
TAGCTATTTTAACGACAGGTAGTCAAGGTGAACCGATGGCTGCTCTATCTAGAATGGCAAAACAAGCACATAAACAAATT
TCAATTCGTAAAGGTGATACTGTTATCATTGCTGCTTCGCCAATTCCGGGTAATGAAATATCTGTATCAAAAATTATTGA
TTTGTTATTTAGAGCTGGAGCTGAAGTTGTTTATTACGGCGAACGAAAAGTTCACGTGTCAGGTCACGGTAGCCAAGAAG
AATTAAAATTAATGTTAAATTTAATGAAACCAAAGTACTTTGTACCCGTACATGGTGAGTTCCGTATGCAAAAAGCACAT
GCATATTTAGCTGAAGATGTAGGTATTACAAGAGAAAATATCTTTATCGTAGAAAAAGGCGATGTAATTGCTTTTGGTGA
CGATGAAGCAAACTTAGTCGGTAAAGTACAAGCTGGTAATGTTTTAATTGATGGATTAGGTGTAGGTGACGTTGGTAATA
TCGTTCTTCGAGATAGAAAGATGTTATCTCAAGATGGAATACTAGTAGTTGTTGTAACGCTTGGTAAAGATGAAAAGAAA
ATTATCTCTGGCCCAGAAATCATTTCACGTGGTTTCGTATATGTTCGTGAATCAGAAGCGTTAATTGAACGATCTACAGA
GATTGTACGTATGATTGTTGAGCAGTCAATTAAAGAATATTCAATTGAATGGTCAATGTTAAAACAAAATATTCGTGAAT
TATTAGGACAGTTCTTGTACGAAGAGACAAAGAGAAAACCTATGATTTTACCGATTATTATGGAAGTATAA

Upstream 100 bases:

>100_bases
GTCTTGGACGGTGAAAATATGGTGGTTGTAGAGTTTCCCCTGGTGTCTCTGCAATTTTAGTGAAATCAGTGTTTGCAAGA
TTTTTAAACCAGGCAACAAC

Downstream 100 bases:

>100_bases
AAAGGTCACCCTTATGGGTGATCTTTTTTCTTTTTAACCCCATCGAATGAAATTTATAACTTAAGAAATAATAGTTATAT
ACGAGTGAAAGGGGATGCGA

Product: metallo-beta-lactamase family protein

Products: NA

Alternate protein names: RNase J2 [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MKRKENESVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIVIPDITYLVENQERVKGLFITHGHEDHI
GGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLKTIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAVV
YTGDFKFDQTPIGNSGADLGKMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQ
QVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLNVPEGMVISLQEVDNFPEKKVAILTTGSQGEPMAALSRMAKQAHKQI
SIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYGERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAH
AYLAEDVGITRENIFIVEKGDVIAFGDDEANLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKK
IISGPEIISRGFVYVRESEALIERSTEIVRMIVEQSIKEYSIEWSMLKQNIRELLGQFLYEETKRKPMILPIIMEV

Sequences:

>Translated_556_residues
MKRKENESVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIVIPDITYLVENQERVKGLFITHGHEDHI
GGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLKTIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAVV
YTGDFKFDQTPIGNSGADLGKMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQ
QVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLNVPEGMVISLQEVDNFPEKKVAILTTGSQGEPMAALSRMAKQAHKQI
SIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYGERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAH
AYLAEDVGITRENIFIVEKGDVIAFGDDEANLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKK
IISGPEIISRGFVYVRESEALIERSTEIVRMIVEQSIKEYSIEWSMLKQNIRELLGQFLYEETKRKPMILPIIMEV
>Mature_556_residues
MKRKENESVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIVIPDITYLVENQERVKGLFITHGHEDHI
GGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLKTIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAVV
YTGDFKFDQTPIGNSGADLGKMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQ
QVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLNVPEGMVISLQEVDNFPEKKVAILTTGSQGEPMAALSRMAKQAHKQI
SIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYGERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAH
AYLAEDVGITRENIFIVEKGDVIAFGDDEANLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKK
IISGPEIISRGFVYVRESEALIERSTEIVRMIVEQSIKEYSIEWSMLKQNIRELLGQFLYEETKRKPMILPIIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Appears to have a limited effect on 16S rRNA maturation, despite its similarity to rnjA [H]

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 60963; Mature: 60963

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKENESVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIVIPDITYL
CCCCCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEEECEECCCCCEEEEEEEECCEEEE
VENQERVKGLFITHGHEDHIGGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLK
ECCCHHEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCEEEEE
TIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAVVYTGDFKFDQTPIGNSGADLG
EECCCCEEEEEEEEEEEECCCCCCCCCCEEEEECCCCCEEEECCCEECCCCCCCCCCCHH
KMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQ
HHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHH
QVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLNVPEGMVISLQEVDNFPEKKVAILTTG
HHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHCCCCCCEEEEEEEC
SQGEPMAALSRMAKQAHKQISIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYG
CCCCHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCEEHHHHHHHHHHCCCCEEEEC
ERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAHAYLAEDVGITRENIFIVEKG
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCEEEHHHHHHHHHHCCCCCCCEEEEECC
DVIAFGDDEANLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKK
CEEEECCCCCCEEEEEEECCEEEEECCCCCCCCEEEECHHHHCCCCEEEEEEEECCCCHH
IISGPEIISRGFVYVRESEALIERSTEIVRMIVEQSIKEYSIEWSMLKQNIRELLGQFLY
HCCCCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EETKRKPMILPIIMEV
HHHCCCCEEEEEEEEC
>Mature Secondary Structure
MKRKENESVKVFALGGVGEIGKNMYCVEIDSEIFIVDAGLMFPGDEMFGIDIVIPDITYL
CCCCCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEEECEECCCCCEEEEEEEECCEEEE
VENQERVKGLFITHGHEDHIGGIVYVLRKLSIPVYATKLTVGLIQEKLGEAGMLGRVDLK
ECCCHHEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCEEEEE
TIDSNSTVEFNTTTVSFFGTTHSIPDSVGVCFHTSKGAVVYTGDFKFDQTPIGNSGADLG
EECCCCEEEEEEEEEEEECCCCCCCCCCEEEEECCCCCEEEECCCEECCCCCCCCCCCHH
KMAQIGNEGVLCLLSDSTNAERPGYTGSEKEVGVEISKVFYGAEGRIIVASFASNVHRIQ
HHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHH
QVFDAAAETGRKVAVVGRSMVKVVDIARRLGYLNVPEGMVISLQEVDNFPEKKVAILTTG
HHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHCCCCCCEEEEEEEC
SQGEPMAALSRMAKQAHKQISIRKGDTVIIAASPIPGNEISVSKIIDLLFRAGAEVVYYG
CCCCHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCEEHHHHHHHHHHCCCCEEEEC
ERKVHVSGHGSQEELKLMLNLMKPKYFVPVHGEFRMQKAHAYLAEDVGITRENIFIVEKG
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCEEEHHHHHHHHHHCCCCCCCEEEEECC
DVIAFGDDEANLVGKVQAGNVLIDGLGVGDVGNIVLRDRKMLSQDGILVVVVTLGKDEKK
CEEEECCCCCCEEEEEEECCEEEEECCCCCCCCEEEECHHHHCCCCEEEEEEEECCCCHH
IISGPEIISRGFVYVRESEALIERSTEIVRMIVEQSIKEYSIEWSMLKQNIRELLGQFLY
HCCCCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EETKRKPMILPIIMEV
HHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]