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The map label for this gene is aacA-aphD [H]

Identifier: 218234229

GI number: 218234229

Start: 2839673

End: 2840209

Strand: Reverse

Name: aacA-aphD [H]

Synonym: BCB4264_A2964

Alternate gene names: 218234229

Gene position: 2840209-2839673 (Counterclockwise)

Preceding gene: 218231123

Following gene: 218235321

Centisome position: 52.41

GC content: 35.2

Gene sequence:

>537_bases
ATGCTTTTTCAAAAAGAAGGTTTATTGATTAGATACGTAATGGAAGATGATGCATCTATCATTTCTAAATGGTTAACGGA
CCCAGCAGTCCTGCGGTATTACGAAGGACGAGATAATCCGCAATCTGTAGAAAAGGTGCTTGATCATTTTATACATAGTC
CAAACAGTAATGAAAAAAGATGTTTAATAGAATTTGATACCATTCCGATTGGTTACATACAAATGTACCCAGTGGATTCA
GAGTGGAAAGCGTTATATGGCTATAAAGAATCACAAAATGTATGGGGTATGGACCAATTTATCGGTGAACCAGCCTATTG
GGGAAAGGGAATTGGAACAAAACTCGTTCAGGCAGCAATTACATATATTATGGGTGAAATGGGAGCAGAAGCAATTGCAA
TGGATCCGAAAGTAAATAATGAACGAGCAATAAAGTGTTATGAAAAAAGTGGATTTAAAAAAGTAAAGATTTTAAAGGAA
CATGAGTTGCATGAGGGGAAATTAGAAGATTGTTGGATGATGGAATATAAACAATAA

Upstream 100 bases:

>100_bases
TACAGCATTTTTTGCGATTACATTACTACCATATGCATTAGTAGGTGCAATGAAAGATGTAACAGCAAGAAAAAATATAA
AGAAATAATAGAGGTATAAT

Downstream 100 bases:

>100_bases
TAATGGCTTATACAAATAAAGGGGGATGTGTGAAATGAAAAAAGCATTAATCGTAATTGATGTGCAAGCGGGTATGTATA
CAGCTGGAATGCCAGTACAT

Product: acetyltransferase, GNAT family

Products: NA

Alternate protein names: 6'-aminoglycoside N-acetyltransferase; AAC(6'); 2''-aminoglycoside phosphotransferase; APH(2'') [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MLFQKEGLLIRYVMEDDASIISKWLTDPAVLRYYEGRDNPQSVEKVLDHFIHSPNSNEKRCLIEFDTIPIGYIQMYPVDS
EWKALYGYKESQNVWGMDQFIGEPAYWGKGIGTKLVQAAITYIMGEMGAEAIAMDPKVNNERAIKCYEKSGFKKVKILKE
HELHEGKLEDCWMMEYKQ

Sequences:

>Translated_178_residues
MLFQKEGLLIRYVMEDDASIISKWLTDPAVLRYYEGRDNPQSVEKVLDHFIHSPNSNEKRCLIEFDTIPIGYIQMYPVDS
EWKALYGYKESQNVWGMDQFIGEPAYWGKGIGTKLVQAAITYIMGEMGAEAIAMDPKVNNERAIKCYEKSGFKKVKILKE
HELHEGKLEDCWMMEYKQ
>Mature_178_residues
MLFQKEGLLIRYVMEDDASIISKWLTDPAVLRYYEGRDNPQSVEKVLDHFIHSPNSNEKRCLIEFDTIPIGYIQMYPVDS
EWKALYGYKESQNVWGMDQFIGEPAYWGKGIGTKLVQAAITYIMGEMGAEAIAMDPKVNNERAIKCYEKSGFKKVKILKE
HELHEGKLEDCWMMEYKQ

Specific function: Resistance to gentamicin, tobramycin, and kanamycin. Tobramycin and kanamycin resistance is due to the ACC activity, specified by N-terminal region, and the gentamicin resistance is due to the APH activity encoded by the C-terminal region of the protein [

COG id: COG1670

COG function: function code J; Acetyltransferases, including N-acetylases of ribosomal proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF01636 APH [H]

EC number: NA

Molecular weight: Translated: 20716; Mature: 20716

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFQKEGLLIRYVMEDDASIISKWLTDPAVLRYYEGRDNPQSVEKVLDHFIHSPNSNEKR
CCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHEECCCCCHHHHHHHHHHHHCCCCCCCCE
CLIEFDTIPIGYIQMYPVDSEWKALYGYKESQNVWGMDQFIGEPAYWGKGIGTKLVQAAI
EEEEECCCCCCEEEEECCCCCHHHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHH
TYIMGEMGAEAIAMDPKVNNERAIKCYEKSGFKKVKILKEHELHEGKLEDCWMMEYKQ
HHHHHHCCCCEEEECCCCCCCCEEEEHHHCCCEEEEHHHHHCCCCCCCCCEEECCCCC
>Mature Secondary Structure
MLFQKEGLLIRYVMEDDASIISKWLTDPAVLRYYEGRDNPQSVEKVLDHFIHSPNSNEKR
CCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHEECCCCCHHHHHHHHHHHHCCCCCCCCE
CLIEFDTIPIGYIQMYPVDSEWKALYGYKESQNVWGMDQFIGEPAYWGKGIGTKLVQAAI
EEEEECCCCCCEEEEECCCCCHHHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHH
TYIMGEMGAEAIAMDPKVNNERAIKCYEKSGFKKVKILKEHELHEGKLEDCWMMEYKQ
HHHHHHCCCCEEEECCCCCCCCEEEEHHHCCCEEEEHHHHHCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3015884; 12663927 [H]