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The map label for this gene is sleB

Identifier: 218234005

GI number: 218234005

Start: 2665098

End: 2665877

Strand: Reverse

Name: sleB

Synonym: BCB4264_A2760

Alternate gene names: 218234005

Gene position: 2665877-2665098 (Counterclockwise)

Preceding gene: 218233532

Following gene: 218230925

Centisome position: 49.19

GC content: 38.59

Gene sequence:

>780_bases
ATGCGCCAAAAAGCTATTTTTAAAATAGCAGTTTTACTTGCGTTCATAGGACTGTCTTTAATGGTCAGTAGTATACAACT
AAAGAATGTAGAAGCTTTTTCTAATCAAGTTATTCAAAGAGGAGCATCTGGCGAAGATGTCATTGAACTGCAATCTCGTT
TGAAATATAACGGATTTTATACGGGAAAAGTGGATGGTGTTTTCGGATGGGGTACATACTGGGCACTTCGGAATTTTCAA
GAGAAATTCGGATTACCCGTTGATGGTTTAGCTGGAGCTAAAACGAAGCAAATGCTCGTGAAGGCAACGAAGTATGACAA
GTCAACTGCTAATAAAGGGACGACAACGAATAAAGGAAATAGCGGCGGAACTGCACAAGAGAATAAACCACCTCAAAATA
AAGGGACAAATGTTCCGAACGGTTATTCTCAAAATGATATTCAGCTTATGGCAAACGCAGTATACGGAGAATCACGTGGT
GAACCGTATTTAGGACAAGTTGCAGTAGCTGCCGTTATTTTGAATCGTGTTACAAGTGCGTCATTCCCAAATACTGTTTC
AGGTGTAATCTTTGAGCCTAGAGCATTTACCGCGGTAGCCGATGGACAAATATATTTAACGCCAAATGAAACAGCAAAAA
AAGCAGTTTTAGATGCGATTAACGGATGGGATCCAACAGGAAATGCTTTATATTATTTCAATCCAGATACTGCGACTAGT
AAATGGATTTGGACTCGTCCACAAATTAAAAAAATCGGTAAACATATTTTCTGTAAATAG

Upstream 100 bases:

>100_bases
CAACGATATTAGTTTGGATTTTTTTGTCTTCTTTACATAAAAGCGAGGCTTTTACAAAACATAACCAAGAAATCATTTCC
GTAATTAAGGAGGGAAAATT

Downstream 100 bases:

>100_bases
AGCGGAGGTGGGACAATGTTACGAGGTATTATCATTGTATTATTAACAGTCGGTGTAGTAGGAACAGGCTATTGGGGCTA
TAAAGAGCACCAAGAGAAAA

Product: spore cortex-lytic enzyme prepeptide

Products: NA

Alternate protein names: SCLE; Germination-specific amidase

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRQKAIFKIAVLLAFIGLSLMVSSIQLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFYTGKVDGVFGWGTYWALRNFQ
EKFGLPVDGLAGAKTKQMLVKATKYDKSTANKGTTTNKGNSGGTAQENKPPQNKGTNVPNGYSQNDIQLMANAVYGESRG
EPYLGQVAVAAVILNRVTSASFPNTVSGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATS
KWIWTRPQIKKIGKHIFCK

Sequences:

>Translated_259_residues
MRQKAIFKIAVLLAFIGLSLMVSSIQLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFYTGKVDGVFGWGTYWALRNFQ
EKFGLPVDGLAGAKTKQMLVKATKYDKSTANKGTTTNKGNSGGTAQENKPPQNKGTNVPNGYSQNDIQLMANAVYGESRG
EPYLGQVAVAAVILNRVTSASFPNTVSGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATS
KWIWTRPQIKKIGKHIFCK
>Mature_259_residues
MRQKAIFKIAVLLAFIGLSLMVSSIQLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFYTGKVDGVFGWGTYWALRNFQ
EKFGLPVDGLAGAKTKQMLVKATKYDKSTANKGTTTNKGNSGGTAQENKPPQNKGTNVPNGYSQNDIQLMANAVYGESRG
EPYLGQVAVAAVILNRVTSASFPNTVSGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATS
KWIWTRPQIKKIGKHIFCK

Specific function: Probable N-acetylmuramyl-L-alanine amidase. Required for spore cortex hydrolysis during germination. May form a complex with some hydrophobic spore component, leading to a stabilization of the enzyme in a spore-bound form

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SLEB_BACCE (P0A3V1)

Other databases:

- EMBL:   D63645
- ProteinModelPortal:   P0A3V1
- SMR:   P0A3V1
- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224
- Gene3D:   G3DSA:1.10.101.10
- TIGRFAMs:   TIGR02869

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1; SSF47090 PGBD_like

EC number: 3.5.1.28

Molecular weight: Translated: 28257; Mature: 28257

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQKAIFKIAVLLAFIGLSLMVSSIQLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEE
TGKVDGVFGWGTYWALRNFQEKFGLPVDGLAGAKTKQMLVKATKYDKSTANKGTTTNKGN
ECCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
SGGTAQENKPPQNKGTNVPNGYSQNDIQLMANAVYGESRGEPYLGQVAVAAVILNRVTSA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
SFPNTVSGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATS
CCCCCCCCEEECCCEEEEEECCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
KWIWTRPQIKKIGKHIFCK
CEEECCHHHHHHHHHHCCC
>Mature Secondary Structure
MRQKAIFKIAVLLAFIGLSLMVSSIQLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEE
TGKVDGVFGWGTYWALRNFQEKFGLPVDGLAGAKTKQMLVKATKYDKSTANKGTTTNKGN
ECCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
SGGTAQENKPPQNKGTNVPNGYSQNDIQLMANAVYGESRGEPYLGQVAVAAVILNRVTSA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
SFPNTVSGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATS
CCCCCCCCEEECCCEEEEEECCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
KWIWTRPQIKKIGKHIFCK
CEEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8752358; 8081503; 10197998