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The map label for this gene is nfdA [H]

Identifier: 218233262

GI number: 218233262

Start: 3372005

End: 3372805

Strand: Reverse

Name: nfdA [H]

Synonym: BCB4264_A3491

Alternate gene names: 218233262

Gene position: 3372805-3372005 (Counterclockwise)

Preceding gene: 218232658

Following gene: 218233965

Centisome position: 62.24

GC content: 35.96

Gene sequence:

>801_bases
ATGGAGTATTTCAAAAAAATTAAGCGTGATGATGTGTCATCCCGTATGCTTATTAATCAAGTGAAAATGTATAGTGATGG
AATTAGTATTAATAGCACTGCTAAAACACTAGAAAAATATAAATTTGAATGGTTTACAGGAACTCCGTATGGGTTGAATT
ATATTCCAGAAGAAAAAATGAAATGGTGGTTAACCGAATTAAATAAAATTGGTTACGGTGCACTTATTCATACAATTGGA
GATGCTGGGGTAAGAGAGAGTTTAAATGCGATTGAAGCAGCGAAAAAAGAAGGTGTAAACCAGCCTTATACACTTACGCA
TGTGGAAATGGTAGAAGATAGTGATATAGATCGTTTTGCTAAGTTAGGTATTAGCACTGATTTTCAAGTAGGTCATGATT
TTGCGGAAGATCCAAAACAAAGCTGGGCAAGTACGTATTTTACAAATCAGCAAATGAAACGAATTATGCCGTTAGGGCGA
ATTTGGAAAACCGGTGCGAATGTATCACTTAGTAGTGATTGGGATGTAAACGAACTGAATCCACTTGTGACGATTTCAAA
TGCATTAAGCATTGATGAAAAGGGCTTGTCTGATGTATACGCAGCTATTGATGCCTATACAATTAAGCCAGCTAAAGCGC
TAGGTCTTGATAACATTACAGGTTCGATTGAAGTAGGCAAATCAGCTGATATGGTATTACTAAATGAAGATATTACTAAA
ATGAGTGCCGATAAGATTCCGAATGCGAAAGTTTTAGTGACGGTTTTACAAGGAGAAGTAGTGCATCACAAGGAGAAATA
A

Upstream 100 bases:

>100_bases
CTGGTTAGACGTTTGGAATAAAGTAAATGACGAAAAAAAGCTAACGTCACGCGTAGTACTTCGACCATGGGTATATCCAA
ATTTAAACGAAGCAGAACAA

Downstream 100 bases:

>100_bases
AGAGTCACCTTATAAAGTTGCTACAAGTAAATGAATATATTCCTGTTGACCTTAACACGATGTCAAAGTATAGACTGGAC
ATACATTCATATAAGGGGTG

Product: urease domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MEYFKKIKRDDVSSRMLINQVKMYSDGISINSTAKTLEKYKFEWFTGTPYGLNYIPEEKMKWWLTELNKIGYGALIHTIG
DAGVRESLNAIEAAKKEGVNQPYTLTHVEMVEDSDIDRFAKLGISTDFQVGHDFAEDPKQSWASTYFTNQQMKRIMPLGR
IWKTGANVSLSSDWDVNELNPLVTISNALSIDEKGLSDVYAAIDAYTIKPAKALGLDNITGSIEVGKSADMVLLNEDITK
MSADKIPNAKVLVTVLQGEVVHHKEK

Sequences:

>Translated_266_residues
MEYFKKIKRDDVSSRMLINQVKMYSDGISINSTAKTLEKYKFEWFTGTPYGLNYIPEEKMKWWLTELNKIGYGALIHTIG
DAGVRESLNAIEAAKKEGVNQPYTLTHVEMVEDSDIDRFAKLGISTDFQVGHDFAEDPKQSWASTYFTNQQMKRIMPLGR
IWKTGANVSLSSDWDVNELNPLVTISNALSIDEKGLSDVYAAIDAYTIKPAKALGLDNITGSIEVGKSADMVLLNEDITK
MSADKIPNAKVLVTVLQGEVVHHKEK
>Mature_266_residues
MEYFKKIKRDDVSSRMLINQVKMYSDGISINSTAKTLEKYKFEWFTGTPYGLNYIPEEKMKWWLTELNKIGYGALIHTIG
DAGVRESLNAIEAAKKEGVNQPYTLTHVEMVEDSDIDRFAKLGISTDFQVGHDFAEDPKQSWASTYFTNQQMKRIMPLGR
IWKTGANVSLSSDWDVNELNPLVTISNALSIDEKGLSDVYAAIDAYTIKPAKALGLDNITGSIEVGKSADMVLLNEDITK
MSADKIPNAKVLVTVLQGEVVHHKEK

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 29818; Mature: 29818

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYFKKIKRDDVSSRMLINQVKMYSDGISINSTAKTLEKYKFEWFTGTPYGLNYIPEEKM
CCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCHHHH
KWWLTELNKIGYGALIHTIGDAGVRESLNAIEAAKKEGVNQPYTLTHVEMVEDSDIDRFA
HHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHH
KLGISTDFQVGHDFAEDPKQSWASTYFTNQQMKRIMPLGRIWKTGANVSLSSDWDVNELN
HHCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
PLVTISNALSIDEKGLSDVYAAIDAYTIKPAKALGLDNITGSIEVGKSADMVLLNEDITK
CEEEECCCCCCCCCCHHHHHHHHHHEEECCHHHHCCCCCCCCEEECCCCCEEEECCCHHH
MSADKIPNAKVLVTVLQGEVVHHKEK
HHHHCCCCCEEEEEEECCCHHHCCCC
>Mature Secondary Structure
MEYFKKIKRDDVSSRMLINQVKMYSDGISINSTAKTLEKYKFEWFTGTPYGLNYIPEEKM
CCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCHHHH
KWWLTELNKIGYGALIHTIGDAGVRESLNAIEAAKKEGVNQPYTLTHVEMVEDSDIDRFA
HHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHH
KLGISTDFQVGHDFAEDPKQSWASTYFTNQQMKRIMPLGRIWKTGANVSLSSDWDVNELN
HHCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
PLVTISNALSIDEKGLSDVYAAIDAYTIKPAKALGLDNITGSIEVGKSADMVLLNEDITK
CEEEECCCCCCCCCCHHHHHHHHHHEEECCHHHHCCCCCCCCEEECCCCCEEEECCCHHH
MSADKIPNAKVLVTVLQGEVVHHKEK
HHHHCCCCCEEEEEEECCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA