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The map label for this gene is nfdA [H]

Identifier: 218232658

GI number: 218232658

Start: 3372976

End: 3373746

Strand: Reverse

Name: nfdA [H]

Synonym: BCB4264_A3492

Alternate gene names: 218232658

Gene position: 3373746-3372976 (Counterclockwise)

Preceding gene: 218234360

Following gene: 218233262

Centisome position: 62.26

GC content: 36.84

Gene sequence:

>771_bases
ATGAGAAGAATAATCATGATTATTTGTCTGATTCTAAGTATGGTATGGATTTCCGCATGTACTTCTGGAGTAGGAGAAGG
TGAGAGTGGTAGCGCTGTTCTACCTAATCAGCTACCTAAAAATGTAGTGTTTAAAAATGGTACAATCTATACTTCAAATG
AAAAGCAAGACATCGTAGAGGCTGTAGCCGTTAAAGAGGGGAAAATTATATTTGTTGGTTCAAACAAGGATGTAGAGGAA
TTTATAAATGAAGATACGAAAGTGTTGGATTTACAAGGACGTTTTATCCTTCCTGGTTTTGTGGACAATCATAATCATAT
ATTTGAAGCAAAATCACCAGCAGCAGGAAATTGTAGTGTTTCACCAGATGCTTCTCTGCAAAAACAACGCTCGTATTTAA
AGGCTTGTAAAAAAGGTGTGAAAGATGGCGGATGGATAAGTGGAACGGGGTTCAGTTTGGAAGCGTTGTTAAAAGATATG
GACGAAAAATCAGAGCAACAAACCCCATTACAAGTTCTTGATGAGTTATTTCCTAATAATCCAGTATTAATTATGGAAAG
AACCTCCCATTCTGTATGGGTTAATTCAAAGGCACTCGAGTTAGCTGGAATAAATAAAGATACAAAAGATCCACAAGGTG
GAAGGATTATTCGGGATTCCGAGACAGGAGAACCGTTTGGTGTTCTTTATGATACAGCAGGAGATATCGTGATGGAAAAA
GCATGGAATTCTCAACCAAATCTGTTTGAAAAAATTATGAAGGTTTATTAG

Upstream 100 bases:

>100_bases
AGTTACGAAAATAGAATGAGAATAAGGATTGACATTAACATTATGTCAATCCTTATTCTATGTGTGAAAGCAATTTATCA
TAATAAAAAGGAGAGAGAAT

Downstream 100 bases:

>100_bases
ACGGTTTAGATGAGGTTGCAAAGAATGGTATTACTACAGTTGGTGATGGACGTCTTTATTGGAAGCGAGGCTGGTTAGAC
GTTTGGAATAAAGTAAATGA

Product: urease domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRRIIMIICLILSMVWISACTSGVGEGESGSAVLPNQLPKNVVFKNGTIYTSNEKQDIVEAVAVKEGKIIFVGSNKDVEE
FINEDTKVLDLQGRFILPGFVDNHNHIFEAKSPAAGNCSVSPDASLQKQRSYLKACKKGVKDGGWISGTGFSLEALLKDM
DEKSEQQTPLQVLDELFPNNPVLIMERTSHSVWVNSKALELAGINKDTKDPQGGRIIRDSETGEPFGVLYDTAGDIVMEK
AWNSQPNLFEKIMKVY

Sequences:

>Translated_256_residues
MRRIIMIICLILSMVWISACTSGVGEGESGSAVLPNQLPKNVVFKNGTIYTSNEKQDIVEAVAVKEGKIIFVGSNKDVEE
FINEDTKVLDLQGRFILPGFVDNHNHIFEAKSPAAGNCSVSPDASLQKQRSYLKACKKGVKDGGWISGTGFSLEALLKDM
DEKSEQQTPLQVLDELFPNNPVLIMERTSHSVWVNSKALELAGINKDTKDPQGGRIIRDSETGEPFGVLYDTAGDIVMEK
AWNSQPNLFEKIMKVY
>Mature_256_residues
MRRIIMIICLILSMVWISACTSGVGEGESGSAVLPNQLPKNVVFKNGTIYTSNEKQDIVEAVAVKEGKIIFVGSNKDVEE
FINEDTKVLDLQGRFILPGFVDNHNHIFEAKSPAAGNCSVSPDASLQKQRSYLKACKKGVKDGGWISGTGFSLEALLKDM
DEKSEQQTPLQVLDELFPNNPVLIMERTSHSVWVNSKALELAGINKDTKDPQGGRIIRDSETGEPFGVLYDTAGDIVMEK
AWNSQPNLFEKIMKVY

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 28210; Mature: 28210

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00135 TRYPSIN_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIIMIICLILSMVWISACTSGVGEGESGSAVLPNQLPKNVVFKNGTIYTSNEKQDIVE
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCEEEECCEEEECCCHHHHHH
AVAVKEGKIIFVGSNKDVEEFINEDTKVLDLQGRFILPGFVDNHNHIFEAKSPAAGNCSV
HHHHCCCCEEEEECCCCHHHHHCCCCEEEEECCCEEECCEECCCCEEEECCCCCCCCCCC
SPDASLQKQRSYLKACKKGVKDGGWISGTGFSLEALLKDMDEKSEQQTPLQVLDELFPNN
CCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCC
PVLIMERTSHSVWVNSKALELAGINKDTKDPQGGRIIRDSETGEPFGVLYDTAGDIVMEK
CEEEEEECCCEEEECCCEEEEECCCCCCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHH
AWNSQPNLFEKIMKVY
HCCCCCHHHHHHHHHC
>Mature Secondary Structure
MRRIIMIICLILSMVWISACTSGVGEGESGSAVLPNQLPKNVVFKNGTIYTSNEKQDIVE
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCEEEECCEEEECCCHHHHHH
AVAVKEGKIIFVGSNKDVEEFINEDTKVLDLQGRFILPGFVDNHNHIFEAKSPAAGNCSV
HHHHCCCCEEEEECCCCHHHHHCCCCEEEEECCCEEECCEECCCCEEEECCCCCCCCCCC
SPDASLQKQRSYLKACKKGVKDGGWISGTGFSLEALLKDMDEKSEQQTPLQVLDELFPNN
CCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCC
PVLIMERTSHSVWVNSKALELAGINKDTKDPQGGRIIRDSETGEPFGVLYDTAGDIVMEK
CEEEEEECCCEEEECCCEEEEECCCCCCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHH
AWNSQPNLFEKIMKVY
HCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA