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The map label for this gene is dacB [H]

Identifier: 218232835

GI number: 218232835

Start: 1459333

End: 1460457

Strand: Direct

Name: dacB [H]

Synonym: BCB4264_A1524

Alternate gene names: 218232835

Gene position: 1459333-1460457 (Clockwise)

Preceding gene: 218232247

Following gene: 218232825

Centisome position: 26.93

GC content: 36.62

Gene sequence:

>1125_bases
ATGAGACGAATTTGTGTAATCATTACGCTTCTTATGATGTATGCAAGCGTTATGCCGATTCCTACATATGCAAAGATGAA
CAGCAATGTTAGTGCTCGAAATGCTGTATTAATGGAGCAACAGTCTGGTCGTGTATTATACGGAAAGGCAGAACATGAGC
CTCAAAAAATTGCTAGTATAACAAAAATTATGACCGCCTTATTAGCTGCTGAATCAGGGAAGATGAAAGAAATGGTATCA
GTTAGTAATGAAGCGGTGAGGGTGGAAGGATCGGCAATCTATTTAAAGCCTGGACAAAAAGTGAAGTTAGAAGATTTAGT
ATACGGACTCATGCTTAGATCTGGTAATGACGCAGCGCAAGTAATTGCTGAAAGTGTGGGTGGGAGTATAGATGGATTCG
TATATTTAATGAATCAAAAAGCGAAAGAAATTGGAATGAAAGATACGCACTTCTCAAACCCTCATGGCTTAGATGGAGAT
GGATCACATTATTCGTCGGCTTACGATATGGCATTATTAACGAAATATGCAATGGGAAACGAGACATTTAAGAAAATCTT
TGGAACCAAAACATATAAATCAGATTCTTGGGATTACCCGTGGAAAAATAAACATAAGCTGGTAACGTCTTATTATGAAT
TTGCAACAGGGGGAAAAACAGGCTTTACGAAGAAAGCTGGGCGAACGCTTGTTACAACGGCATCAAAAGGTGGACTAGAT
TTAATTGTCGTAACTTTGAGTGCCTCTAGTGATTGGGATGATCATATGAATTTGTTTGATAAAGGTTTTGACCGTTTCAA
ACAAACGAAAGTTTTAGGACAAGGAGCAATTGCTGAAATAAATGAAAAGAAGTATGCCAACCATGTTTATACGAAAAATA
GTTTTTCGGTACCGTTAACTGAAGAAGAAAAAAAGAACGTAGTATTAAAAGTTGAACTCGATAAAAGTACAAAACTTACG
GATGGAGTAAAGGTTGGGAAGACAGACATTTATGTTGGTAATGAAAAAGTTGGAGAACGGAATTTATTTTATAGTAAACG
AAAGTTAGTAGCTACAACGGGACTTTACTGGAATAATGTGAAAGAAATTTTTTCTTACATGATAGGTGTTGGGAACGATG
GTTAA

Upstream 100 bases:

>100_bases
TGCGTCTTTTTTAATGGAAGTAGAAGGAAGATTTCGTTCATATTTTAAAAGGCCAAGCATACACTTGTACAAAAAGTGCC
AAAAGGACGTGGGGGGAAAT

Downstream 100 bases:

>100_bases
TCTCGTATGGGTAGCGATGGCTGTCATTGGGATTGTATACGCCATGATAAATGGAACGATGGAAGAAGTGAATAAAGCTG
TATTTGACGGAGCAAAAGAT

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRRICVIITLLMMYASVMPIPTYAKMNSNVSARNAVLMEQQSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKEMVS
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAESVGGSIDGFVYLMNQKAKEIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTKYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKGGLD
LIVVTLSASSDWDDHMNLFDKGFDRFKQTKVLGQGAIAEINEKKYANHVYTKNSFSVPLTEEEKKNVVLKVELDKSTKLT
DGVKVGKTDIYVGNEKVGERNLFYSKRKLVATTGLYWNNVKEIFSYMIGVGNDG

Sequences:

>Translated_374_residues
MRRICVIITLLMMYASVMPIPTYAKMNSNVSARNAVLMEQQSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKEMVS
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAESVGGSIDGFVYLMNQKAKEIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTKYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKGGLD
LIVVTLSASSDWDDHMNLFDKGFDRFKQTKVLGQGAIAEINEKKYANHVYTKNSFSVPLTEEEKKNVVLKVELDKSTKLT
DGVKVGKTDIYVGNEKVGERNLFYSKRKLVATTGLYWNNVKEIFSYMIGVGNDG
>Mature_374_residues
MRRICVIITLLMMYASVMPIPTYAKMNSNVSARNAVLMEQQSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKEMVS
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAESVGGSIDGFVYLMNQKAKEIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTKYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKGGLD
LIVVTLSASSDWDDHMNLFDKGFDRFKQTKVLGQGAIAEINEKKYANHVYTKNSFSVPLTEEEKKNVVLKVELDKSTKLT
DGVKVGKTDIYVGNEKVGERNLFYSKRKLVATTGLYWNNVKEIFSYMIGVGNDG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=296, Percent_Identity=34.7972972972973, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87082022, Length=379, Percent_Identity=28.7598944591029, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786851, Length=262, Percent_Identity=33.587786259542, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082054, Length=214, Percent_Identity=27.1028037383178, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41468; Mature: 41468

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRICVIITLLMMYASVMPIPTYAKMNSNVSARNAVLMEQQSGRVLYGKAEHEPQKIASI
CCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHEEEEECCCCCEEEECCCCCHHHHHHH
TKIMTALLAAESGKMKEMVSVSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQ
HHHHHHHHHHCCCCHHHHHHCCCCEEEEECCEEEECCCCEEEHHHHHHHHHHCCCCHHHH
VIAESVGGSIDGFVYLMNQKAKEIGMKDTHFSNPHGLDGDGSHYSSAYDMALLTKYAMGN
HHHHHHCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKGGLD
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCEE
LIVVTLSASSDWDDHMNLFDKGFDRFKQTKVLGQGAIAEINEKKYANHVYTKNSFSVPLT
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCCC
EEEKKNVVLKVELDKSTKLTDGVKVGKTDIYVGNEKVGERNLFYSKRKLVATTGLYWNNV
CCCCCCEEEEEEECCCCCCCCCEEECCEEEEECCCCCCCCCHHHHCCCEEEECCCHHHHH
KEIFSYMIGVGNDG
HHHHHHHHCCCCCC
>Mature Secondary Structure
MRRICVIITLLMMYASVMPIPTYAKMNSNVSARNAVLMEQQSGRVLYGKAEHEPQKIASI
CCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHEEEEECCCCCEEEECCCCCHHHHHHH
TKIMTALLAAESGKMKEMVSVSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQ
HHHHHHHHHHCCCCHHHHHHCCCCEEEEECCEEEECCCCEEEHHHHHHHHHHCCCCHHHH
VIAESVGGSIDGFVYLMNQKAKEIGMKDTHFSNPHGLDGDGSHYSSAYDMALLTKYAMGN
HHHHHHCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKGGLD
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCEE
LIVVTLSASSDWDDHMNLFDKGFDRFKQTKVLGQGAIAEINEKKYANHVYTKNSFSVPLT
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCCC
EEEKKNVVLKVELDKSTKLTDGVKVGKTDIYVGNEKVGERNLFYSKRKLVATTGLYWNNV
CCCCCCEEEEEEECCCCCCCCCEEECCEEEEECCCCCCCCCHHHHCCCEEEECCCHHHHH
KEIFSYMIGVGNDG
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]