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The map label for this gene is pbpF [H]

Identifier: 218232390

GI number: 218232390

Start: 1076052

End: 1078169

Strand: Direct

Name: pbpF [H]

Synonym: BCB4264_A1102

Alternate gene names: 218232390

Gene position: 1076052-1078169 (Clockwise)

Preceding gene: 218230824

Following gene: 218233350

Centisome position: 19.86

GC content: 39.0

Gene sequence:

>2118_bases
ATGAAGAAAGTAAAGTGGACTTTATTAGGCGGCGTCGCAACGTTCGTAGTAGCGATTGTAATCTATAAACTTATCGTGTT
AGCTGGTGGCTATATGATGGATGAAAAGCAGCTCGTTTTCCACTCTTCATCACGTATCGTTGACCAGAAAGGGAAAGAAA
TTACGAAATTATACGTAGAAAATAGAGAGCTCGTACCGATCGAGCAAATTCCGAAGTATGTGCAGCAGGCGTTTGTTGCG
GTGGAAGATTCTCGTTTTTATGAGCATCAAGGGATTGATTATCCGTCTATATTCCGTGCTCTTTATAAAGATACGTTAGC
TAGAGAGAAGGTAGAGGGCGGTAGTACCATTACGCAGCAACTCGCTAAAAACGTCTTTTTAACTCGTGAAAAAACATTTA
CGCGCAAATTGAAGGAAGTCGCAATTTCTCTTCAATTAGAGCAAAAATATACGAAGCAACAAATTCTTGAAATGTATATG
AATCATATTTATTTTGGTCATGGGGCTTACGGTATTCAAGCGGCAGCAAAGTTGTATTTTAATAAAAATGTTGAGGATTT
AACAGTAGAGGAAGGGGCAATGCTTGCAGGCCTTCCGAAATCACCAAACGGATATTCACCGTACTATTCTGCAGAGAAGA
GTAAGGAACGCCGTGATCTAGTGTTGTCACTTATGCATAAACAAGGCTATTTGACTGCAGAAGAAAGCGTTCGTTATCAA
GGGAAGACAATTGCTCTATATAAAAATTTAGATGAGAATGAGCTTGCATATATGCCGTATATTGATATGGTCATAGATGA
AGCCGCTCGTTTATACGGGTTGTCTCATCAAGAAGTACTTCGCGGAGGATATACGTTTGTCGTACCTATGGATGAAAAGA
TTCAAAAGGTAGCATATAACCAGTTTCAAGATGCAAGGAATTTCCCTGGGAAAGAAGAGGGGGCGCAAGGTGCTTTCTTA
TTAATGGATAATCGTACGGGAGGGATTAAAGCGGCGATTGGGGGAAGAAAGTACGTCCCGAGAGGATTTAATCGTGTTTT
TGCCAAAAGGCAGCCTGGATCTGTTTTAAAACCACTTATCGTTTATGCACCAGCACTAGAAACGAAAAAGTATAATCCGT
ATTCTTTATTAACGAACGAAAGAAATTCTTTTGAAGGGTATGAACCTCGAAATTATAATCATGAGTATTCAAAAGAAATG
ACGATGTATGATGCGATTTTAGAGTCGGCAAATGTACCGGCAGTTTCTTTATTAAATGAGTTAGGGGTAGAAGAAGGAAA
GCAATATTTAGAAAAAGGAAATGTTCATATTGCAGATGCTGGTTTAAGTACAGCGCTTGGCGGATTGAAAAATGGCGTTT
CAACATTTGATCTTGTAAAAATGTATCGTTCATTTTTAGCAAATGGAAATATTATCGAGCCGCACGTTATTGATAAAGTA
TTAAATAGACACGGTGCAGTCATTGGGGAATCTCCAAAAGTAGAGACAAAAATCTTCTCGAAGCAGACAGCATGGTATAT
GACGAAAATGTTAGAAGGAGTTGTGAAGGAAGGAACAGCGAGAGTTGGTGTATATAACGGTGCGTTAGCTGGAAAAACGG
GTACAACTTCATTGCCAAATGACGATAAGGGAGCAAGAGATATGTGGTTCGTTGGTTATACACCGAACTTAGTAGGCGCT
GTTTGGATTGGTTATGACCGTACGGATAAGGAGCATCAGTTGCAAGGAGAAAGTGCATCTGCGACAAAATTGTTTAAGAA
AATTTTAACGAAGTCTAATGTAGAACATAAAGAGAAGTTTATGAAGCCTGAAGGTGTGGAAACAATCAGCGCTCCGATTC
GGCTGCGCAAAATTGAAGATGTAAAAATGAAATTATCATTTAGCCCTTTCGGTTTATTTAAAGCAAAATTAAGCTGGACG
CCACTTCCAGATAACAGAATTATGTACCGAATCTATAGAGTGGAGAACGGAATTCATACGCATGTTAGCACTGTAAATGG
TGCTGGGGAATATGAGGAAAAGTTCGTTAACATATTTTCAAAACCGAGCTTTTACGTTGTACCATATAATACACAAACGA
ATCGTGAAGGAGAAAAGTCAAAAGTAGCTAAACCATAG

Upstream 100 bases:

>100_bases
TATTATATCAACTCTCTTAAAAAACTTCGAAGATTATATACATAAAGATACTTCTTTTTCAAATAATAAATGTACCATCA
TTTGGAAAGAAGGACGATGT

Downstream 100 bases:

>100_bases
TTTTTCTCTGTGTTATAATAAGAATGTTGTGAAAATAGTAAGCGTTTTTAAGAATGTTTGTGTCAATTTAATGAACAACG
TACATAATATTATGCATTTT

Product: penicillin-binding protein 1

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MKKVKWTLLGGVATFVVAIVIYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVENRELVPIEQIPKYVQQAFVA
VEDSRFYEHQGIDYPSIFRALYKDTLAREKVEGGSTITQQLAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYM
NHIYFGHGAYGIQAAAKLYFNKNVEDLTVEEGAMLAGLPKSPNGYSPYYSAEKSKERRDLVLSLMHKQGYLTAEESVRYQ
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFL
LMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYAPALETKKYNPYSLLTNERNSFEGYEPRNYNHEYSKEM
TMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRSFLANGNIIEPHVIDKV
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTARVGVYNGALAGKTGTTSLPNDDKGARDMWFVGYTPNLVGA
VWIGYDRTDKEHQLQGESASATKLFKKILTKSNVEHKEKFMKPEGVETISAPIRLRKIEDVKMKLSFSPFGLFKAKLSWT
PLPDNRIMYRIYRVENGIHTHVSTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP

Sequences:

>Translated_705_residues
MKKVKWTLLGGVATFVVAIVIYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVENRELVPIEQIPKYVQQAFVA
VEDSRFYEHQGIDYPSIFRALYKDTLAREKVEGGSTITQQLAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYM
NHIYFGHGAYGIQAAAKLYFNKNVEDLTVEEGAMLAGLPKSPNGYSPYYSAEKSKERRDLVLSLMHKQGYLTAEESVRYQ
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFL
LMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYAPALETKKYNPYSLLTNERNSFEGYEPRNYNHEYSKEM
TMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRSFLANGNIIEPHVIDKV
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTARVGVYNGALAGKTGTTSLPNDDKGARDMWFVGYTPNLVGA
VWIGYDRTDKEHQLQGESASATKLFKKILTKSNVEHKEKFMKPEGVETISAPIRLRKIEDVKMKLSFSPFGLFKAKLSWT
PLPDNRIMYRIYRVENGIHTHVSTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP
>Mature_705_residues
MKKVKWTLLGGVATFVVAIVIYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVENRELVPIEQIPKYVQQAFVA
VEDSRFYEHQGIDYPSIFRALYKDTLAREKVEGGSTITQQLAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYM
NHIYFGHGAYGIQAAAKLYFNKNVEDLTVEEGAMLAGLPKSPNGYSPYYSAEKSKERRDLVLSLMHKQGYLTAEESVRYQ
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFL
LMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYAPALETKKYNPYSLLTNERNSFEGYEPRNYNHEYSKEM
TMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRSFLANGNIIEPHVIDKV
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTARVGVYNGALAGKTGTTSLPNDDKGARDMWFVGYTPNLVGA
VWIGYDRTDKEHQLQGESASATKLFKKILTKSNVEHKEKFMKPEGVETISAPIRLRKIEDVKMKLSFSPFGLFKAKLSWT
PLPDNRIMYRIYRVENGIHTHVSTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=249, Percent_Identity=43.3734939759036, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1786343, Length=566, Percent_Identity=28.6219081272085, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1788867, Length=563, Percent_Identity=26.1101243339254, Blast_Score=150, Evalue=4e-37,
Organism=Escherichia coli, GI1789601, Length=222, Percent_Identity=32.4324324324324, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 79691; Mature: 79691

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKWTLLGGVATFVVAIVIYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVE
CCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEECCCHHHHHCCCHHHHHHCC
NRELVPIEQIPKYVQQAFVAVEDSRFYEHQGIDYPSIFRALYKDTLAREKVEGGSTITQQ
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYMNHIYFGHGAYGIQAAAKLYF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
NKNVEDLTVEEGAMLAGLPKSPNGYSPYYSAEKSKERRDLVLSLMHKQGYLTAEESVRYQ
CCCCCEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEHHHHHHCC
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYN
CCEEEEEECCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHCCCEEEEECHHHHHHHHHH
QFQDARNFPGKEEGAQGAFLLMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLI
HHHHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHH
VYAPALETKKYNPYSLLTNERNSFEGYEPRNYNHEYSKEMTMYDAILESANVPAVSLLNE
HCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRSFLANGNIIEPHVIDKV
HCHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECHHHHHHH
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTARVGVYNGALAGKTGTTSLPN
HHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCC
DDKGARDMWFVGYTPNLVGAVWIGYDRTDKEHQLQGESASATKLFKKILTKSNVEHKEKF
CCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHH
MKPEGVETISAPIRLRKIEDVKMKLSFSPFGLFKAKLSWTPLPDNRIMYRIYRVENGIHT
CCCCCCCHHCCCEEEEEEECEEEEEECCCCCEEEEEEECCCCCCCCEEEEEEEECCCCEE
HVSTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP
EEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKVKWTLLGGVATFVVAIVIYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVE
CCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEECCCHHHHHCCCHHHHHHCC
NRELVPIEQIPKYVQQAFVAVEDSRFYEHQGIDYPSIFRALYKDTLAREKVEGGSTITQQ
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYMNHIYFGHGAYGIQAAAKLYF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
NKNVEDLTVEEGAMLAGLPKSPNGYSPYYSAEKSKERRDLVLSLMHKQGYLTAEESVRYQ
CCCCCEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEHHHHHHCC
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYN
CCEEEEEECCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHCCCEEEEECHHHHHHHHHH
QFQDARNFPGKEEGAQGAFLLMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLI
HHHHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHH
VYAPALETKKYNPYSLLTNERNSFEGYEPRNYNHEYSKEMTMYDAILESANVPAVSLLNE
HCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRSFLANGNIIEPHVIDKV
HCHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEECHHHHHHH
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTARVGVYNGALAGKTGTTSLPN
HHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCC
DDKGARDMWFVGYTPNLVGAVWIGYDRTDKEHQLQGESASATKLFKKILTKSNVEHKEKF
CCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHH
MKPEGVETISAPIRLRKIEDVKMKLSFSPFGLFKAKLSWTPLPDNRIMYRIYRVENGIHT
CCCCCCCHHCCCEEEEEEECEEEEEECCCCCEEEEEEECCCCCCCCEEEEEEEECCCCEE
HVSTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP
EEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]