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The map label for this gene is thiF [H]

Identifier: 218232275

GI number: 218232275

Start: 767385

End: 768404

Strand: Direct

Name: thiF [H]

Synonym: BCB4264_A0790

Alternate gene names: 218232275

Gene position: 767385-768404 (Clockwise)

Preceding gene: 218233508

Following gene: 218232781

Centisome position: 14.16

GC content: 35.88

Gene sequence:

>1020_bases
TTGAATAATCGATATTCTCGCCAAGAACTATTTTCTCCAATTGGAGAAGAAGGACAGCAAAAGATAAGAGAAAAGCATGT
GCTTATTATCGGTGCAGGTGCATTAGGTAGTGCAAATGCAGAGATGTTTGTAAGAGCAGGTGTTGGCAAGATAACAATTG
TTGACCGTGATTATGTAGATTGGAGTAATTTACAAAGACAACAATTGTATGCAGAGAGCGACGTAAAGAATAATCTTCCA
AAAGCTATAGCGGCTAAAAAACGTTTAGAAGAGATTAATAGTGATGTAACAATAGAAGCTCTCGTTCAAGATGTAACAGC
TGAAGAGCTAGAAGAACTTGTTATAAATGTTGATGTAATTATTGATGCGACTGATAATTTCGAAACGCGCTTTATTGTAA
ATGATATATCACAAAAATATTCTATTCCATGGATTTACGGTGCATGTGTAGGTAGTTACGGTCTTTCTTACACAATCCTC
CCTAGTAAAACACCATGTTTATCATGTTTATTACAGTCGATTCCGCTTGGCGGGGCAACATGTGATACAGCGGGTATTAT
ATCACCTGCTGTATCTCTTGTCGTTTCTCATCAAGTAACAGAAGCTCTTAAACTGTTAGTAGAAGATTACGAATCACTTC
GAGATGGGCTGGTATCGTTTGATATGTGGAAGAATGAGTATTCATGTATGAATGTACAAAAACTTCGCCAGCATAATTGT
CCTTCATGCGGAGAGAATGCATTATATCCGTATTTAAATAAAGAAAACACATCAAAAACAGCAGTTTTATGCGGGCGAAA
TACAGTTCAAATTAGACCACCTCATAAAGATGAAATGAATTTTGAACAATATAAAGAGTTGTTGGAAGGTCGTGTAAATG
ATTTAAATATAAATCCATATTTACTATCATTTTCTGTTGAAGACAAGAGGTTAGTAGCCTTTAAAGATGGCCGTGTGCTC
GTACATGGAACGAAAGATATAAGCGAAGCAAAAACGATTTATCATCGCTATTTTGGATAG

Upstream 100 bases:

>100_bases
AAATTAAGTATTGAAGCAGGCCGTTTAGGATTTGAAGCAGGTCGCATTGCACGTAAGCGTTGTGCAACTGCAAGTAGTCC
TTTAGAAGGAATGAGCGTAG

Downstream 100 bases:

>100_bases
AAAAGGATGAGTGGGATGAAAGTGAATAAAGCTTTAACAATTGCAGGGTCTGATAGCGGCGGCGGTGCTGGAATTCAGGC
AGATTTAAAAACATTCCAAG

Product: thiamine/molybdopterin biosynthesis ThiF/MoeB-like protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNNRYSRQELFSPIGEEGQQKIREKHVLIIGAGALGSANAEMFVRAGVGKITIVDRDYVDWSNLQRQQLYAESDVKNNLP
KAIAAKKRLEEINSDVTIEALVQDVTAEELEELVINVDVIIDATDNFETRFIVNDISQKYSIPWIYGACVGSYGLSYTIL
PSKTPCLSCLLQSIPLGGATCDTAGIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDMWKNEYSCMNVQKLRQHNC
PSCGENALYPYLNKENTSKTAVLCGRNTVQIRPPHKDEMNFEQYKELLEGRVNDLNINPYLLSFSVEDKRLVAFKDGRVL
VHGTKDISEAKTIYHRYFG

Sequences:

>Translated_339_residues
MNNRYSRQELFSPIGEEGQQKIREKHVLIIGAGALGSANAEMFVRAGVGKITIVDRDYVDWSNLQRQQLYAESDVKNNLP
KAIAAKKRLEEINSDVTIEALVQDVTAEELEELVINVDVIIDATDNFETRFIVNDISQKYSIPWIYGACVGSYGLSYTIL
PSKTPCLSCLLQSIPLGGATCDTAGIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDMWKNEYSCMNVQKLRQHNC
PSCGENALYPYLNKENTSKTAVLCGRNTVQIRPPHKDEMNFEQYKELLEGRVNDLNINPYLLSFSVEDKRLVAFKDGRVL
VHGTKDISEAKTIYHRYFG
>Mature_339_residues
MNNRYSRQELFSPIGEEGQQKIREKHVLIIGAGALGSANAEMFVRAGVGKITIVDRDYVDWSNLQRQQLYAESDVKNNLP
KAIAAKKRLEEINSDVTIEALVQDVTAEELEELVINVDVIIDATDNFETRFIVNDISQKYSIPWIYGACVGSYGLSYTIL
PSKTPCLSCLLQSIPLGGATCDTAGIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDMWKNEYSCMNVQKLRQHNC
PSCGENALYPYLNKENTSKTAVLCGRNTVQIRPPHKDEMNFEQYKELLEGRVNDLNINPYLLSFSVEDKRLVAFKDGRVL
VHGTKDISEAKTIYHRYFG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=289, Percent_Identity=28.0276816608997, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI38045948, Length=172, Percent_Identity=30.2325581395349, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI150417996, Length=161, Percent_Identity=31.055900621118, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=35.7429718875502, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1787048, Length=243, Percent_Identity=30.0411522633745, Blast_Score=121, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17540406, Length=246, Percent_Identity=32.520325203252, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17557754, Length=146, Percent_Identity=30.8219178082192, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=249, Percent_Identity=28.9156626506024, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320598, Length=188, Percent_Identity=32.9787234042553, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24582879, Length=247, Percent_Identity=28.7449392712551, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 38081; Mature: 38081

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRYSRQELFSPIGEEGQQKIREKHVLIIGAGALGSANAEMFVRAGVGKITIVDRDYVD
CCCCCCHHHHHHHCCHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCC
WSNLQRQQLYAESDVKNNLPKAIAAKKRLEEINSDVTIEALVQDVTAEELEELVINVDVI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEE
IDATDNFETRFIVNDISQKYSIPWIYGACVGSYGLSYTILPSKTPCLSCLLQSIPLGGAT
EECCCCCCEEEEEEHHHHHCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCC
CDTAGIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDMWKNEYSCMNVQKLRQHNC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCHHCCHHHHHHCCC
PSCGENALYPYLNKENTSKTAVLCGRNTVQIRPPHKDEMNFEQYKELLEGRVNDLNINPY
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEE
LLSFSVEDKRLVAFKDGRVLVHGTKDISEAKTIYHRYFG
EEEEECCCCEEEEEECCEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNNRYSRQELFSPIGEEGQQKIREKHVLIIGAGALGSANAEMFVRAGVGKITIVDRDYVD
CCCCCCHHHHHHHCCHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCC
WSNLQRQQLYAESDVKNNLPKAIAAKKRLEEINSDVTIEALVQDVTAEELEELVINVDVI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEE
IDATDNFETRFIVNDISQKYSIPWIYGACVGSYGLSYTILPSKTPCLSCLLQSIPLGGAT
EECCCCCCEEEEEEHHHHHCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCC
CDTAGIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDMWKNEYSCMNVQKLRQHNC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCHHCCHHHHHHCCC
PSCGENALYPYLNKENTSKTAVLCGRNTVQIRPPHKDEMNFEQYKELLEGRVNDLNINPY
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEE
LLSFSVEDKRLVAFKDGRVLVHGTKDISEAKTIYHRYFG
EEEEECCCCEEEEEECCEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]