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The map label for this gene is dppE [H]

Identifier: 218231950

GI number: 218231950

Start: 3611946

End: 3613661

Strand: Reverse

Name: dppE [H]

Synonym: BCB4264_A3695

Alternate gene names: 218231950

Gene position: 3613661-3611946 (Counterclockwise)

Preceding gene: 218233091

Following gene: 218232568

Centisome position: 66.68

GC content: 35.26

Gene sequence:

>1716_bases
ATGAAGAGAAAAAAGATGAAAAAGTTAACGGCAGTTGTAGTACCAGTTTTAGCGATGAGTGTTGCATTGACAGCATGTTC
TGGGTCAGGTGGGGAAAAGAAAACGACTACTACATCAAATAGTGGGGAAGAAAAAAAATCTGATATTAAATATGCAGCGA
AACAAGTATTAAATCGTACGGAAAACCAAGAAATTCCGACGATGGATACTTCTAAATCTACTGATACATTAGGTGCGCAA
ATTTTAGGGAACACGATGGAAGGCCTATATCGCCTTGATAAAGATAATAAGCCAATTCCTGCTGCGGCAGAGTCTAGTAC
GAAAAGTGAAGATGGTAAAAAGTATACGTTTAAGCTGCGAAAAGATGCAAAATGGTCAAATGGTGATCCTGTAACAGCGA
AAGATTTCGTTTTCGCATGGCAGCGCTTACTTGATAAGAATACTGCGGCAGAATATGCATTTATTGCTTACTATATTAAA
AATGCGGAAGCGATTAATAAAGGAGAAAAACCTGCAACTGAATTAGGAGCGAAGGCGGTAGATGATTACACGCTGGAAGT
AGAATTAGAAAAACCGGTACCGTATTTCTTAAATTTATTAGCATTCCCATCATACTATCCATTAAATGAAAAATTCGTAA
AAGAAAAAGGTGATAAGTACGGTTTAGAAGCAGATACAACTGTGTATAACGGTCCATTCGTTATGTCTTCATGGAAACAT
GAACAAGGTTGGCAGCTGAAGAAAAATGATAAGTACTGGGATAAAAAGACTGTGAAATTAGAAGAAATTAACTATAGTGT
AGTAAAAGAGGTTGCAACGAAAGTAAACTTATATGATACAGGATCAATTGATTTTACATTACTATCAGGAGAGTTTGTAG
ATAAATATAAATCGAACAAAGAAGAATATGGTGAGTATGCGGAATCAAGTACATTCTTCTTACGTTTAAATCAAAAACGT
AACGGACAAGACACGCCTTTAAAGAGCAAAAAACTTCGTGAAGCAATTGCATTATCAGTTGATAAAAAAGGATTGGCAAA
TGTAATTTTAAATAATGGATCAAAAGCAACAGATCAATTGGTTCCAAAAGGACTTGCGACAGGGCCAGATGGTAAAGATT
ATCAAGATACATTTAAAAATGGTCTGAAATATAATCCGAAAAAAGGAGCAGCAGCGTGGGAAGAAGCGAAAAAAGAACTT
GGAAAAGATCAAGTTACAATTGAATTGCTAAGTTATGATGATGGAACTGCGAAAAAGATTGCTGACTATGTAAAAGATCA
AATTGAGAAAAATTTAAAAGGTGTAACGATTAATACAAAAATTCAGCCGTTCAAACAAAAATTAAAATTAGAAACTGCGC
AAGATTATGAAATTTCTTATGCAGGTTGGAGTCCAGACTATGCGGATCCAATGACATTTATTGATATGTTTGAATCGAAG
AGCCCGTACAACCAAATGAGTTATTCAAATCCAAAATACGATGAAATGGTAGGAAAAGCAGGTAATGAATTAATGAGTGA
TGCGAAAAAACGCTGGGAAACGTTAGGGAAAGCTGAAAAGTTATTCCTTGAAGAAGATGCAGGGTTAGTTCCTTTATATC
AAACAGGAAGATCGTACGTAATGAAACCGAATGTAAAAGGAATTGTGAAACATAACATCAGTCCAGAATATAGCTTTAAG
TGGGCGTATGTAACTGAGGATGGCGGAAAAAAATAA

Upstream 100 bases:

>100_bases
AGAGTAGAATAACATTAGTTGTAATTTTCTGAAAAACGAATCTTTAGTAAGGGGTCTCTCAAGGGTTGGTAATGTACATA
TAATTAGGGAGGGTACAAAA

Downstream 100 bases:

>100_bases
AGTGAAAGCATTTCAAAAGTGGCTGAGCTGCTTTTGAAATGCTTTTTAGTATGCATAAGGGAATAAATCTCGTTAGCCTA
TTGAGAATCGGGTAGGTAAT

Product: oligopeptide-binding protein OppA

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MKRKKMKKLTAVVVPVLAMSVALTACSGSGGEKKTTTTSNSGEEKKSDIKYAAKQVLNRTENQEIPTMDTSKSTDTLGAQ
ILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVFAWQRLLDKNTAAEYAFIAYYIK
NAEAINKGEKPATELGAKAVDDYTLEVELEKPVPYFLNLLAFPSYYPLNEKFVKEKGDKYGLEADTTVYNGPFVMSSWKH
EQGWQLKKNDKYWDKKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYAESSTFFLRLNQKR
NGQDTPLKSKKLREAIALSVDKKGLANVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYNPKKGAAAWEEAKKEL
GKDQVTIELLSYDDGTAKKIADYVKDQIEKNLKGVTINTKIQPFKQKLKLETAQDYEISYAGWSPDYADPMTFIDMFESK
SPYNQMSYSNPKYDEMVGKAGNELMSDAKKRWETLGKAEKLFLEEDAGLVPLYQTGRSYVMKPNVKGIVKHNISPEYSFK
WAYVTEDGGKK

Sequences:

>Translated_571_residues
MKRKKMKKLTAVVVPVLAMSVALTACSGSGGEKKTTTTSNSGEEKKSDIKYAAKQVLNRTENQEIPTMDTSKSTDTLGAQ
ILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVFAWQRLLDKNTAAEYAFIAYYIK
NAEAINKGEKPATELGAKAVDDYTLEVELEKPVPYFLNLLAFPSYYPLNEKFVKEKGDKYGLEADTTVYNGPFVMSSWKH
EQGWQLKKNDKYWDKKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYAESSTFFLRLNQKR
NGQDTPLKSKKLREAIALSVDKKGLANVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYNPKKGAAAWEEAKKEL
GKDQVTIELLSYDDGTAKKIADYVKDQIEKNLKGVTINTKIQPFKQKLKLETAQDYEISYAGWSPDYADPMTFIDMFESK
SPYNQMSYSNPKYDEMVGKAGNELMSDAKKRWETLGKAEKLFLEEDAGLVPLYQTGRSYVMKPNVKGIVKHNISPEYSFK
WAYVTEDGGKK
>Mature_571_residues
MKRKKMKKLTAVVVPVLAMSVALTACSGSGGEKKTTTTSNSGEEKKSDIKYAAKQVLNRTENQEIPTMDTSKSTDTLGAQ
ILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVFAWQRLLDKNTAAEYAFIAYYIK
NAEAINKGEKPATELGAKAVDDYTLEVELEKPVPYFLNLLAFPSYYPLNEKFVKEKGDKYGLEADTTVYNGPFVMSSWKH
EQGWQLKKNDKYWDKKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYAESSTFFLRLNQKR
NGQDTPLKSKKLREAIALSVDKKGLANVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYNPKKGAAAWEEAKKEL
GKDQVTIELLSYDDGTAKKIADYVKDQIEKNLKGVTINTKIQPFKQKLKLETAQDYEISYAGWSPDYADPMTFIDMFESK
SPYNQMSYSNPKYDEMVGKAGNELMSDAKKRWETLGKAEKLFLEEDAGLVPLYQTGRSYVMKPNVKGIVKHNISPEYSFK
WAYVTEDGGKK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=511, Percent_Identity=30.7240704500978, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789397, Length=509, Percent_Identity=30.0589390962672, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI87081878, Length=535, Percent_Identity=26.9158878504673, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1787762, Length=461, Percent_Identity=27.765726681128, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787052, Length=507, Percent_Identity=23.2741617357002, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789887, Length=339, Percent_Identity=25.9587020648968, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789966, Length=475, Percent_Identity=23.5789473684211, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64247; Mature: 64247

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKKMKKLTAVVVPVLAMSVALTACSGSGGEKKTTTTSNSGEEKKSDIKYAAKQVLNRT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
ENQEIPTMDTSKSTDTLGAQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEE
KDAKWSNGDPVTAKDFVFAWQRLLDKNTAAEYAFIAYYIKNAEAINKGEKPATELGAKAV
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHEEEHHEECCHHHHCCCCCCHHHHCCHHC
DDYTLEVELEKPVPYFLNLLAFPSYYPLNEKFVKEKGDKYGLEADTTVYNGPFVMSSWKH
CCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCEEECCCCC
EQGWQLKKNDKYWDKKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNK
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCEEECCCEEEEEECCHHHHHHHCCH
EEYGEYAESSTFFLRLNQKRNGQDTPLKSKKLREAIALSVDKKGLANVILNNGSKATDQL
HHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHEECCCCHHHHHH
VPKGLATGPDGKDYQDTFKNGLKYNPKKGAAAWEEAKKELGKDQVTIELLSYDDGTAKKI
CCCCCCCCCCCCCHHHHHHCCCEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHH
ADYVKDQIEKNLKGVTINTKIQPFKQKLKLETAQDYEISYAGWSPDYADPMTFIDMFESK
HHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCC
SPYNQMSYSNPKYDEMVGKAGNELMSDAKKRWETLGKAEKLFLEEDAGLVPLYQTGRSYV
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEECCCCEEEHHHCCCCEE
MKPNVKGIVKHNISPEYSFKWAYVTEDGGKK
ECCCCCCCEECCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MKRKKMKKLTAVVVPVLAMSVALTACSGSGGEKKTTTTSNSGEEKKSDIKYAAKQVLNRT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
ENQEIPTMDTSKSTDTLGAQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEE
KDAKWSNGDPVTAKDFVFAWQRLLDKNTAAEYAFIAYYIKNAEAINKGEKPATELGAKAV
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHEEEHHEECCHHHHCCCCCCHHHHCCHHC
DDYTLEVELEKPVPYFLNLLAFPSYYPLNEKFVKEKGDKYGLEADTTVYNGPFVMSSWKH
CCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCEEECCCCC
EQGWQLKKNDKYWDKKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNK
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCEEECCCEEEEEECCHHHHHHHCCH
EEYGEYAESSTFFLRLNQKRNGQDTPLKSKKLREAIALSVDKKGLANVILNNGSKATDQL
HHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHEECCCCHHHHHH
VPKGLATGPDGKDYQDTFKNGLKYNPKKGAAAWEEAKKELGKDQVTIELLSYDDGTAKKI
CCCCCCCCCCCCCHHHHHHCCCEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHH
ADYVKDQIEKNLKGVTINTKIQPFKQKLKLETAQDYEISYAGWSPDYADPMTFIDMFESK
HHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCC
SPYNQMSYSNPKYDEMVGKAGNELMSDAKKRWETLGKAEKLFLEEDAGLVPLYQTGRSYV
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEECCCCEEEHHHCCCCEE
MKPNVKGIVKHNISPEYSFKWAYVTEDGGKK
ECCCCCCCEECCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]